Home Products Cited in Publications Worldwide Expression, purification, and characterization of anuran saxiphilins using thermofluor, fluorescence polarization, and isothermal titration calorimetry
STAR Protoc.,2024,5(1):102792.
Chen, Zhou; Zakrzewska, Sandra; Hajare, Holly S.; Du Bois, J.; Minor, Daniel L. Jr.
DOI:10.1016/j.xpro.2023.102792 PMID:38133955
Anuran saxiphilins (Sxphs) are “toxin sponge” proteins thought to prevent the lethal effects of small-molecule neurotoxins through sequestration. Here, we present a protocol for the expression, purification, and characterization of Sxphs. We describe steps for using thermofluor, fluorescence polarization, and isothermal titration calorimetry assays that probe Sxph:saxitoxin interactions using a range of sample quantities. These assays are generalizable and can be used for other paralytic shellfish poisoning toxin-binding proteins.