Marta Stolarek; Kamil Kaminski; Marta Kaczor-Kamińska; Magdalena Obłoza; Piotr Bonarek; Anna Czaja; Magdalena Datta; Wojciech Łach; Mateusz Brela; Artur Sikorski; Janusz Rak; Maria Nowakowska; Krzysztof Szczubiałka

DOI: PMID:

Abstract

A photoactive analogue of was synthesized with two arylazopyrazole ligands, able to undergo trans−cis/cis−trans photoisomerizations. The cis photoisomer showed a dark half-life of 9 days. The cytotoxicities of both photoisomers of the complex were determined in several cancer and normal cell lines and compared to that of . The trans photoisomer of the complex was much more cytotoxic than both the cis photoisomer and , and was more toxic for cancer (4T1) than for normal (NMuMG) murine breast cells. 4T1 cell death occurred through necrosis. Photoisomerization of the trans and cis photoisomers internalized by the 4T1 cells increased and decreased their viability, respectively. The cellular uptake of the trans photoisomer was stronger than that of both the cis photoisomer and . Both photoisomers interacted with DNA faster than . The trans photoisomer was bound stronger by bovine serum albumin and induced a greater decrease in cellular levels than the cis photoisomer.

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