Products Cited in Publications Worldwide An endogenous aryl hydrocarbon receptor ligand induces preeclampsia-like phenotypes in rats

An endogenous aryl hydrocarbon receptor ligand induces preeclampsia-like phenotypes in rats

Zhao, Ying‐Jie; Zhou, Chi; Zhang, Si‐Yan; Mishra, Jay S; Li, Hui‐Hui; Lei, Wei; Wang, Kai; Kumar, Sathish; Zheng, Jing

DOI: PMID:

Abstract

Preeclampsia (PE) is a hypertensive disorder during human pregnancy. Aryl hydro-carbon receptor (AhR) is a ligand-activated transcription factor. Exogenous and endogenous AhR ligands can induce hypertension in male rats and mice. Herein, using rats as a model, we tested the hypothesis that over-regulation of endogenous AhR ligands during pregnancy impairs vascular functions by disrupting the transcriptome in the placenta, contributing to the development of PE. Pregnant rats were injected daily with an endogenous AhR ligand, 2-(1'H-indole-3'-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE), from gestational day (GD) 10 to 19. Maternal mean blood pressure was measured on GD16–20. Proteinuria and uteroplacental blood flow were monitored on GD20. Placentas collected on GD20 were used to determine changes in vascular density and transcriptome. Compared with the vehicle control, ITE elevated maternal mean blood pressure by 22% and 16% on GD16 and 17, respectively. ITE increased proteinuria by 50% and decreased uteroplacental blood flow by 26%. ITE reduced the placental vascular density by 18%. RNA sequencing analysis revealed that ITE induced 1316 and 2020 differentially expressed genes (DEGs) in female and male placentas, respectively. These DEGs were enriched in pathways relevant to heart diseases, vascular functions and inflammation. Bioinformatics analysis also predicted that ITE altered immune cell infiltration in placentas depending on fetal sex. These data suggest that over-regulation of endogenous AhR ligands may lead to PE with impaired vascular functions and disrupted fetal sex-specific transcriptomes and immune cell infiltration in placentas. These AhR ligand-induced DEGs and pathways may represent promising therapeutic targets for PE-induced cardiovascular dysfunctions.

Keywords

AhR ligand ; placentas ; preeclampsia ; sexual dimorphism ; trans_x005f_x0002_criptomics ; vasculature

Purchased from AmBeed

ITE

A163287448906-42-198%

ITE

19.0/5mg

Detail