Products Cited in Publications Worldwide Phosphodiesterase 4 Inhibitor Plays An Anti-fibrosis Effect in Ligamentum Flavum Fibroblasts

Phosphodiesterase 4 Inhibitor Plays An Anti-fibrosis Effect in Ligamentum Flavum Fibroblasts

Wu, Likang; Song, Guoqiang

DOI: PMID:

Abstract

Objective: Phosphodiesterase 4 inhibitor has anti‐inflammatory and anti‐fibrosis effects. We applied Rolipram, a phosphodiesterase 4 inhibitor,to ligamentum flavum fibroblasts to investigate its anti‐fibrotic effect and involved signaling pathways. Methods: Transforming growth factor‐β1 (TGF‐β1) was used to stimulate fibroblasts of the ligamentum flavum to make a fibrotic cell model. Fibrotic cells were treated with Rolipram and WAY‐262611. Western blotting was used to detect the expression of TGF‐ β1, collagen I (Col I) and collagen III in the cells and to examine changes in β‐catenin signaling pathways. Results: TGF‐β1 stimulates fibroblast fibrosis and increase the expression of collagen I, collagen III and endogenous TGF‐β1. Rolipram blocks this change, which is related to the suppression of activated p‐β‐catenin. Conclusion: Rolipram has a good anti‐fibrosis effectin vitro, which may be beneficialto the treatment of ligamentum flavum hypertrophy. We propose a new idea here, but a lot of research is needed for its specific application.

Keywords

Phosphodiesterase ; Ligamentum flavum ; Fibrosis ; TGF‐β1

Purchased from AmBeed

WAY-262611

A3188311123231-07-195%

WAY-262611

294.0/50mg

Detail