Products Cited in Publications Worldwide Quantitative Monitoring of Solvent Concentration and Moisture Content in Flow Synthesis

Quantitative Monitoring of Solvent Concentration and Moisture Content in Flow Synthesis

Hattori, Yusuke; Suzuki, Yasuyuki

Abstract

In this work, with the aim of quantitatively monitoring solvent concentration and moisture content in synthesis processes, a micro-flow cell and regression model of near-infrared (NIR) spectra were developed and utilized for monitoring peptide synthesis. The flow cell was designed to have sufficient resistance to pressure and sufficient displacement efficiency of the reaction fluid. The regression models employed the partial least squares regression method. The quantified concentration of each solvent and moisture content were equivalent to the theoretical values with low standard deviations. The standard deviation of moisture content was 10 ppm. Even a tiny amount of water can have a critical adverse impact on some chemical reactions. Therefore, strict control of moisture content is required. The proposed method for monitoring solvent concentration and moisture content can be simultaneously conducted with monitoring of other reaction factors, such as reaction products. The present results indicate the utility of this method for in-line spectroscopic monitoring of synthesis processes.

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Fmoc-Oic-OH

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