Name: 1323290-64-7|Methyl 4,5-diamino-5-oxopentanoate hydrochloride|95%
Brand: Ambeed
SKU: A409909
Price: 91.0 USD
Availability: InStock
Rating: 94 (28 reviews)

1
[ 1323407-17-5 ]
[ 1323290-64-7 ]
[ 1323407-19-7 ]

Yield	Reaction Conditions	Operation in experiment
76%	With N-ethyl-N,N-diisopropylamine In acetonitrile at 60℃; for 16h; Inert atmosphere;
	With N-ethyl-N,N-diisopropylamine In acetonitrile at 20 - 40℃; for 24h;	6.1.4 6.1.4 4-Carbamoyl-butyric acid methyl ester 6.1.4 4-Carbamoyl-butyric acid methyl ester To a stirred solution of methyl 2-(bromomethyl)-3-(tert-butyldimethylsilyloxy)benzoate (137.5 g, 325 mmol) in acetonitrile (1100 mL) in a 2 L round bottom flask, was added methyl 4,5-diamino-5-oxopentanoate hydrochloride (70.4 g, 358 mmol). To the suspension was added DIPEA (119 ml, 683 mmol) through an addition funnel over 10 minutes and the suspension was stirred at room temperature for 1 hour before the mixture was heated in an oil bath at 40° C. for 23 hours. The reaction mixture was concentrated under vacuo. The residue was stirred in ether (600 mL), and a white solid precipitated out. The mixture was filtered and the solid was washed with ether (400 mL). The filtrate was washed with HCl (1N, 200 mL), NaHCO3 (sat. 200 mL) and brine (250 mL). The aqueous acid layer and basic layer were kept separately. Then the solid was further washed with ether (250 mL) and the liquid was washed with above acid solution and basic solution. The two organic layers were combined and concentrated under vacuo to give 4-[4-(tert-Butyl-dimethyl-silanyloxy)-1-oxo-1,3-dihydro-isoindol-2-yl]-4-carbamoyl-butyric acid methyl ester as a brown oil (152 g, 115% crude yield, 77% purity by H NMR). The compound was used in the next step without further purification: LCMS MH=407.
	With N-ethyl-N,N-diisopropylamine In acetonitrile at 20 - 40℃; for 24.16h;	1.4 4-Carbamoyl-butyric acid methyl ester To a stirred solution of methyl 2-(bromomethyl)-3-(tert-butyldimethylsilyloxy)benzoate (137.5 g, 325 mmol) in acetonitrile (1100 mL) in a 2 L round bottom flask, was added methyl 4,5-diamino-5-oxopentanoate hydrochloride (70.4 g, 358 mmol). To the suspension was added DIPEA (119 ml, 683 mmol) through an addition funnel over 10 minutes and the suspension was stirred at room temperature for 1 hour before the mixture was heated in an oil bath at 40° C. for 23 hours. The reaction mixture was concentrated under vacuo. The residue was stirred in ether (600 mL), and a white solid precipitated out. The mixture was filtered and the solid was washed with ether (400 mL). The filtrate was washed with HCl (1N, 200 mL), NaHCO3 (sat. 200 mL) and brine (250 mL). The aqueous acid layer and basic layer were kept separately. Then the solid was further washed with ether (250 mL) and the liquid was washed with above acid solution and basic solution. The two organic layers were combined and concentrated under vacuo to give 4-[4-(tert-Butyl-dimethyl-silanyloxy)-1-oxo-1,3-dihydro-isoindol-2-yl]-4-carbamoyl-butyric acid methyl ester as a brown oil (152 g, 115% crude yield, 77% purity by 1H NMR). The compound was used in the next step without further purification: LCMS MH=407.

	With N-ethyl-N,N-diisopropylamine In acetonitrile at 20 - 40℃; for 24h;	6.4 4-Carbamoyl-butyric acid methyl ester To a stirred cold solution of methyl 5-amino-4-(4-(tert-butyldimethylsilyloxy)-1-oxoisoindolin-2-yl)-5-oxopentanoate (152 g, 288 mmol) in DMF (500 mL) and water (55 mL), was added by K2CO3 (19.89 g, 144 mmol) by portions over 5 minutes. The resulting reaction mixture was stirred at room temperature for 40 minutes. The reaction mixture was cooled in an ice bath. To the mixture, HCl (12M, 23.99 ml, 288 mmol) was added slowly. After the addition, acetonitrile (280 mL) was added to the mixture and a solid precipitated out. The mixture was stirred at room temperature for 10 minutes and filtered. The solid was washed with acetonitrile (50 mL x 4). The filtrate was concentrated under high vacuo to give a yellow oil (168 g). The oil was dissolved in acetonitrile (600 mL) and stirred at room temperature for 10minutes. The mixture was filtered and the solid was washed with acetonitrile (25 mL x 2). The filtrate was concentrated under high vacuo to give a yellow oil (169g), which was added to a mixture of water (1200 mL) and ether (1000 mL). The mixture was stirred for 3 minutes and the layers were separated. The aqueous solution was concentrated under high vacuo and the residue was stirred in acetonitrile (160 mL) and a white solid was formed after overnight stirring. The mixture was filtered to give 4- carbamoyl-4-(4-hydroxy-1-oxo-1,3-dihydro-isoindol-2-yl)-butyric acid methyl ester as a white solid (46 g, 54% yield). The filtrate was concentrated and the residue was further crystallized in acetonitrile (60 mL) to give more 4-carbamoyl-4-(4-hydroxy-1-oxo-1,3- dihydro-isoindol-2-yl)-butyric acid methyl ester as a white solid( 11.7 g, 14% yield). The filtrate was concentrated and the residue was purified by ISCO chromatography to give more 4-carbamoyl-4-(4-hydroxy-1-oxo-1,3-dihydro-isoindol -2-yl)-butyric acid methyl ester as a white solid (13.2 g, 15% yield). The total product obtained was 70.9 g in 83% yield: LCMS MH = 293; 1H NMR (DMSO-d6) δ 1.95 - 2.34 (m, 4H, CH2, CH2), 3.51 (s, 3H, CH3), 4.32 (d, J= 17.6 Hz, 1H, CHH), 4.49 (d, J= 17.4 Hz, 1H, CHH), 4.73 (dd, J = 4.7, 10.2 Hz, 1H, CHH), 6.99 (dd, J= 0.8, 7.9 Hz, 1H, Ar), 7.10 - 7.23 (m, 2H, Ar, NHH), 7.25 - 7.38 (m, 1H, Ar), 7.58 (s, 1H, NHH), 10.04 (s, 1H, OH).
	In acetonitrile at 0 - 50℃; for 5h;
	With N-ethyl-N,N-diisopropylamine In acetonitrile at 60℃; for 16h;	1 Methyl 5-amino-4-[4-[tert-butyl(dimethyl)silyl]oxy-1-oxo-isoindolin-2-yl]-5-oxo-pentanoate To a stirred solution of methyl 4,5-diamino-5-oxo-pentanoate hydrochloride (74.5 g, 379 mmol) in acetonitrile (2.50 L) was added methyl 2-(bromomethyl)-3-[tert-butyl(dimethyl)silyl] oxy-benzoate (125 g, 348 mmol). To the suspension was added diisopropylethylamine (89.9 g, 696 mmol) through an addition funnel over 10 min and then the mixture was stirred at 60° C. for 16 h. The reaction mixture was diluted with ethyl acetate (1.0 L), washed with HCl (1N, 1.0 L), sodium bicarbonate (sat. 1.0 L) and brine (1.0 L) successively. The organic layer was concentrated to give crude methyl 5-amino-4-[4-[tert-butyl(dimethyl)silyl]oxy-1-oxo-isoindolin-2-yl]-5-oxo-pentanoate (108 g, crude) as a light yellow solid. LCMS: m/z 407.3 [M+1]+.
	With N-ethyl-N,N-diisopropylamine In acetonitrile at 60℃; for 16.166h;	1 Methyl 5-amino-4-[4-[tert-butyl(dimethyl)silyl]oxy-l-oxoisoindolin-2- yl]-5-oxo-pentanoate. To a stirred solution of methyl 4,5-diamino-5-oxo-pentanoate hydrochloride (74.5 g, 379 mmol) in acetonitrile (2.50 L) was added methyl 2- (bromomethyl)-3-[tert-butyl(dimethyl)silyl] oxy-benzoate (125 g, 348 mmol). To the suspension was added diisopropylethylamine (89.9 g, 696 mmol) through an addition funnel over 10 min and then the mixture was stirred at 60 °C for 16 h. The reaction mixture was diluted with ethyl acetate (1.0 L), washed with HC1 (IN, 1.0 L), sodium bicarbonate (sat.1.0 L) and brine (1.0 L) successively. The organic layer was concentrated to give crude methyl 5-amino-4-[4-[tert-butyl(dimethyl)silyl]oxy-l-oxo- isoindolin-2-yl]- 5-oxo-pentanoate (108 g, crude) as a light yellow solid. LCMS: m/z 407.3 [M+l]+.
	With N-ethyl-N,N-diisopropylamine In acetonitrile at 60℃; for 16h;	1 Methyl 5-amino-4-[4-[tert-butyl(dimethyl)silyl]oxy-1-oxo-isoindolin-2-yl]-5-oxo-pentanoate Methyl 5-amino-4-[4-[tert-butyl(dimethyl)silyl]oxy-1-oxo-isoindolin-2-yl]-5-oxo-pentanoate To a stirred solution of methyl 4,5-diamino-5-oxo-pentanoate hydrochloride (74.5 g, 379 mmol) in acetonitrile (2.50 L) was added methyl 2-(bromomethyl)-3-[tert-butyl(dimethyl)silyl]oxy-benzoate (125 g, 348 mmol). To the suspension was added diisopropylethylamine (89.9 g, 696 mmol) through an addition funnel over 10 min and then the mixture was stirred at 60° C. for 16 h. The reaction mixture was diluted with ethyl acetate (1.0 L), washed with HCl (1N, 1.0 L), sodium bicarbonate (sat. 1.0 L) and brine (1.0 L) successively. The organic layer was concentrated to give crude methyl 5-amino-4-[4-[tert-butyl(dimethyl)silyl]oxy-1-oxo-isoindolin-2-yl]-5-oxo-pentanoate (108 g, crude) as a light yellow solid. LCMS: m/z 407.3 [M+1]+.
	With N-ethyl-N,N-diisopropylamine In acetonitrile at 40℃; for 23h;	6.1.4 (4) 4-Carbamoyl-butyric acid methyl ester To a stirred solution of methyl 2-(bromomethyl)-3-(tert - butyldimethylsilyloxy )benzoate (137.5 g, 325 mmol) in acetonitrile (1100 mL) in a 2 L round bottom flask, was added methyl 4,5-diamino-5-oxopentanoate hydrochloride (70.4 g, 358 mmol). To the suspension was added DIPEA (119 ml, 683 mmol) through an addition funnel over 10 minutes and the suspension was stirred at room temperature for 1 hour before the mixture was heated in an oil bath at 40°C for 23 hours. The reaction mixture was concentrated under vacuo. The residue was stirred in ether (600 mL), and a white solid precipitated out. The mixture was filtered and the solid was washed with ether (400 mL). The filtrate was washed with HC1 (IN, 200 mL), NaHCOs (sat. 200 mL) and brine (250 mL). The aqueous acid layer and basic layer were kept separately. Then the solid was further washed with ether (250 mL) and the liquid was washed with above acid solution and basic solution. The two organic layers were combined and concentrated under vacuo to give 4-[4- (tert -Butyl-dimethyl-silanyloxy)-l-oxo-l,3-dihydro-isoindol-2-yl]-4-carbamoyl-butyric acid methyl ester as a brown oil (152g, 115% crude yield, 77% purity by H NMR). The compound was used in the next step without further purification: LCMS MH = 407.
	With N-ethyl-N,N-diisopropylamine In acetonitrile at 40℃; for 23h;	6.1.4 (4) 4-Carbamoyl-butyric acid methyl ester To a stirred solution of methyl 2-(bromomethyl)-3-(tert - butyldimethylsilyloxy )benzoate (137.5 g, 325 mmol) in acetonitrile (1100 mL) in a 2 L round bottom flask, was added methyl 4,5-diamino-5-oxopentanoate hydrochloride (70.4 g, 358 mmol). To the suspension was added DIPEA (119 ml, 683 mmol) through an addition funnel over 10 minutes and the suspension was stirred at room temperature for 1 hour before the mixture was heated in an oil bath at 40°C for 23 hours. The reaction mixture was concentrated under vacuo. The residue was stirred in ether (600 mL), and a white solid precipitated out. The mixture was filtered and the solid was washed with ether (400 mL). The filtrate was washed with HC1 (IN, 200 mL), NaHCOs (sat. 200 mL) and brine (250 mL). The aqueous acid layer and basic layer were kept separately. Then the solid was further washed with ether (250 mL) and the liquid was washed with above acid solution and basic solution. The two organic layers were combined and concentrated under vacuo to give 4-[4- (tert -Butyl-dimethyl-silanyloxy)-l-oxo-l,3-dihydro-isoindol-2-yl]-4-carbamoyl-butyric acid methyl ester as a brown oil (152g, 115% crude yield, 77% purity by H NMR). The compound was used in the next step without further purification: LCMS MH = 407.

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]
[2]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2014/45843, 2014, A1 Location in patent: Paragraph 0417
[3]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2014/45844, 2014, A1 Location in patent: Paragraph 0287; 0288
[4]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - WO2014/39960, 2014, A1 Location in patent: Page/Page column 62
[5]Current Patent Assignee: ARVINAS - WO2018/140809, 2018, A1 Location in patent: Paragraph 00393
[6]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1 Location in patent: Paragraph 0112; 0316
[7]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - WO2019/226761, 2019, A1 Location in patent: Paragraph 00275; 00282
[8]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/361005, 2019, A1 Location in patent: Paragraph 1068
[9]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - WO2022/72576, 2022, A1 Location in patent: Paragraph 00291
[10]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - WO2022/72576, 2022, A1 Location in patent: Paragraph 00291

2
[ 1323290-64-7 ]
[ 1323407-16-4 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 2 steps 1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C 2: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C
	Multi-step reaction with 2 steps 1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C 2: potassium carbonate; water / N,N-dimethyl-formamide / 15 h / 15 °C
	Multi-step reaction with 2 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere

Reference： [1]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1
[2]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/361005, 2019, A1
[3]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

3
[ 1323290-64-7 ]
[ 1323407-22-2 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 3 steps 1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C 2: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C 3: potassium carbonate / acetonitrile / 16 h / 60 °C
	Multi-step reaction with 3 steps 1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C 2: potassium carbonate; water / N,N-dimethyl-formamide / 15 h / 15 °C 3: potassium carbonate / acetonitrile / 16 h / 60 °C
	Multi-step reaction with 3 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere

Reference： [1]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1
[2]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/361005, 2019, A1
[3]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

4
[ 1323290-64-7 ]
[ 1323407-86-8 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 4 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -70 °C 4.2: -70 - 20 °C
	Multi-step reaction with 4 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C 2.1: potassium carbonate; water / N,N-dimethyl-formamide / 15 h / 15 °C 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -70 °C 4.2: -70 °C
	Multi-step reaction with 4 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice

Reference： [1]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1
[2]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/361005, 2019, A1
[3]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

5
[ 1323290-64-7 ]
[ 1323404-89-2 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / N,N-dimethyl-formamide / 16 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / 0 °C / Inert atmosphere 5.1: sulfuric acid / tetrahydrofuran / 2 h / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

6
[ 1323290-64-7 ]
[ 1323410-28-1 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 4 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

7
[ 1323290-64-7 ]
[ 1323405-74-8 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

8
[ 1323290-64-7 ]
[ 1323405-76-0 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

9
[ 1323290-64-7 ]
[ 1323406-46-7 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 18 h / 40 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

10
[ 1323290-64-7 ]
[ 1323406-54-7 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 18 h / 40 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

11
[ 1822563-16-5 ]
[ 1323290-64-7 ]

Yield	Reaction Conditions	Operation in experiment
95%	With hydrogenchloride In ethyl acetate at 25℃; for 12h;	1 Methyl 4,5-diamino-5-oxo-pentanoate hydrochloride A mixture of methyl 5-amino-4-(tert-butoxycarbonylamino)-5-oxo-pentanoate (180 g, 692 mmol) and HCl/ethyl acetate (300 mL, 4 M) was stirred at 25° C. for 12 h. The precipitated solid was collected by vacuum filtration and washed with ethyl acetate (500 mL) to give methyl 4,5-diamino-5-oxo-pentanoate hydrochloride (130 g, 95% yield) as a white solid. 1H NMR: 400 MHz CD3OD δ: 4.00-3.96 (m, 1H), 3.70 (s, 3H), 2.59-2.52 (m, 2H), 2.22-2.13 (m, 2H).
95%	With hydrogenchloride In ethyl acetate at 25℃; for 12h;	1 Methyl 4, 5-diamino- 5-oxo-pentanoate hydrochloride A mixture of methyl 5-amino-4-(tert-butoxycarbonylamino)-5-oxo-pentanoate (180 g, 692 mmol) and HCl/ethyl acetate (300 mL, 4 M) was stirred at 25 °C for 12 h. The precipitated solid was collected by vacuum filtration and washed with ethyl acetate (500 mL) to give methyl 4,5-diamino-5-oxo-pentanoate hydrochloride (130 g, 95 % yield) as a white solid. (0496) ^ NMR: 400 MHz CD3OD d: 4.00-3.96 (m, 1H), 3.70 (s, 3H), 2.59-2.52 (m, 2H), 2.22-2.13 (m, 2H).
95%	With hydrogenchloride In ethyl acetate at 25℃; for 12h;	1 Methyl 4,5-diamino-5-oxo-pentanoate hydrochloride Methyl 4,5-diamino-5-oxo-pentanoate hydrochloride A mixture of methyl 5-amino-4-(tert-butoxycarbonylamino)-5-oxo-pentanoate (180 g, 692 mmol) and HCl/ethyl acetate (300 mL, 4 M) was stirred at 25° C. for 12 h. The precipitated solid was collected by vacuum filtration and washed with ethyl acetate (500 mL) to give methyl 4,5-diamino-5-oxo-pentanoate hydrochloride (130 g, 95% yield) as a white solid. 1H NMR: 400 MHz CD3OD δ: 4.00-3.96 (m, 1H), 3.70 (s, 3H), 2.59-2.52 (m, 2H), 2.22-2.13 (m, 2H).

95%

With hydrogenchloride In ethyl acetate at 25℃; for 12h; Inert atmosphere;

Reference： [1]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1 Location in patent: Paragraph 0312
[2]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - WO2019/226761, 2019, A1 Location in patent: Paragraph 00275; 00278
[3]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/361005, 2019, A1 Location in patent: Paragraph 1064
[4]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

12
[ 1323290-64-7 ]
[ 2260745-31-9 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -70 °C 4.2: -70 - 20 °C 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 18 h / 40 °C
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C 2.1: potassium carbonate; water / N,N-dimethyl-formamide / 15 h / 15 °C 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -70 °C 4.2: -70 °C 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 18 h / 40 °C
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 18 h / 40 °C / Inert atmosphere

Reference： [1]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1
[2]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/361005, 2019, A1
[3]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

13
[ 14487-45-7 ]
[ 1323290-64-7 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 3 steps 1.1: triethylamine / methanol / 2 h / 25 °C 2.1: pyridine; di-<i>tert</i>-butyl dicarbonate / 1,4-dioxane / 0.5 h / 25 °C 2.2: 16 h / 25 °C 3.1: hydrogenchloride / ethyl acetate / 12 h / 25 °C
	Multi-step reaction with 3 steps 1.1: triethylamine / methanol / 2 h / 25 °C 2.1: pyridine / 1,4-dioxane / 0.5 h / 25 °C 2.2: 16 h / 25 °C 3.1: hydrogenchloride / ethyl acetate / 12 h / 25 °C
	Multi-step reaction with 3 steps 1.1: triethylamine / 2 h / 25 °C / Inert atmosphere 2.1: di-<i>tert</i>-butyl dicarbonate; pyridine / 1,4-dioxane / 0.5 h / 25 °C / Inert atmosphere 2.2: 16 h / 25 °C / Inert atmosphere 3.1: hydrogenchloride / ethyl acetate / 12 h / 25 °C / Inert atmosphere

Reference： [1]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1
[2]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/361005, 2019, A1
[3]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

14
[ 617-65-2 ]
[ 1323290-64-7 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 4 steps 1.1: chloro-trimethyl-silane / methanol / 0.5 h / 20 °C / Inert atmosphere 2.1: triethylamine / methanol / 2 h / 25 °C 3.1: pyridine; di-<i>tert</i>-butyl dicarbonate / 1,4-dioxane / 0.5 h / 25 °C 3.2: 16 h / 25 °C 4.1: hydrogenchloride / ethyl acetate / 12 h / 25 °C
	Multi-step reaction with 4 steps 1.1: chloro-trimethyl-silane / 0.5 h / 20 °C / Inert atmosphere 2.1: triethylamine / methanol / 2 h / 25 °C 3.1: pyridine / 1,4-dioxane / 0.5 h / 25 °C 3.2: 16 h / 25 °C 4.1: hydrogenchloride / ethyl acetate / 12 h / 25 °C
	Multi-step reaction with 4 steps 1.1: chloro-trimethyl-silane / 0.5 h / 20 °C / Inert atmosphere 2.1: triethylamine / 2 h / 25 °C / Inert atmosphere 3.1: di-<i>tert</i>-butyl dicarbonate; pyridine / 1,4-dioxane / 0.5 h / 25 °C / Inert atmosphere 3.2: 16 h / 25 °C / Inert atmosphere 4.1: hydrogenchloride / ethyl acetate / 12 h / 25 °C / Inert atmosphere

Reference： [1]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1
[2]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/361005, 2019, A1
[3]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

15
[ 112159-16-7 ]
methyl 4,5-diamino-5-oxo-pentanoate hydrochloride [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 2 steps 1.1: pyridine; di-<i>tert</i>-butyl dicarbonate / 1,4-dioxane / 0.5 h / 25 °C 1.2: 16 h / 25 °C 2.1: hydrogenchloride / ethyl acetate / 12 h / 25 °C
	Multi-step reaction with 2 steps 1.1: pyridine / 1,4-dioxane / 0.5 h / 25 °C 1.2: 16 h / 25 °C 2.1: hydrogenchloride / ethyl acetate / 12 h / 25 °C
	Multi-step reaction with 2 steps 1.1: di-<i>tert</i>-butyl dicarbonate; pyridine / 1,4-dioxane / 0.5 h / 25 °C / Inert atmosphere 1.2: 16 h / 25 °C / Inert atmosphere 2.1: hydrogenchloride / ethyl acetate / 12 h / 25 °C / Inert atmosphere

Reference： [1]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1
[2]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/361005, 2019, A1
[3]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

16
[ 1323290-64-7 ]
[ 2260745-37-5 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -70 °C 4.2: -70 - 20 °C 5.1: N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 4 h / 20 °C

Reference： [1]Current Patent Assignee: BRISTOL-MYERS SQUIBB CO - US2019/8852, 2019, A1

17
[ 1323290-64-7 ]
[ 2586195-65-3 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 8 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / N,N-dimethyl-formamide / 16 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / 0 °C / Inert atmosphere 5.1: sulfuric acid / tetrahydrofuran / 2 h / 20 °C / Inert atmosphere 6.1: potassium carbonate / N,N-dimethyl-formamide / 5 h / 0 - 15 °C / Inert atmosphere 7.1: N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 1 h / -5 °C / Inert atmosphere 8.1: N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 16 h / 25 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

18
[ 1323290-64-7 ]
[ 2586194-31-0 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 7 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / N,N-dimethyl-formamide / 16 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / 0 °C / Inert atmosphere 5.1: sulfuric acid / tetrahydrofuran / 2 h / 20 °C / Inert atmosphere 6.1: potassium carbonate / N,N-dimethyl-formamide / 5 h / 0 - 15 °C / Inert atmosphere 7.1: N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 1 h / -5 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

19
[ 1323290-64-7 ]
[ 2586195-75-5 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 3 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: 1-methyl-pyrrolidin-2-one; potassium carbonate / 4 h / 25 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

20
[ 1323290-64-7 ]
[ 2586194-34-3 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 4 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: 1-methyl-pyrrolidin-2-one; potassium carbonate / 4 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 1.5 h / -70 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

21
[ 1323290-64-7 ]
[ 2586196-41-8 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 6 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: 1-methyl-pyrrolidin-2-one; potassium carbonate / 4 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 1.5 h / -70 °C / Inert atmosphere 5.1: trifluoroacetic acid / dichloromethane / 12 h / 25 °C / Inert atmosphere 6.1: N-ethyl-N,N-diisopropylamine / tetrahydrofuran / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

22
[ 1323290-64-7 ]
[ 2586196-46-3 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: 1-methyl-pyrrolidin-2-one; potassium carbonate / 4 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 1.5 h / -70 °C / Inert atmosphere 5.1: trifluoroacetic acid / dichloromethane / 12 h / 25 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

23
[ 1323290-64-7 ]
[ 2586196-39-4 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 7 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: 1-methyl-pyrrolidin-2-one; potassium carbonate / 4 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 1.5 h / -70 °C / Inert atmosphere 5.1: trifluoroacetic acid / dichloromethane / 12 h / 25 °C / Inert atmosphere 6.1: N-ethyl-N,N-diisopropylamine / tetrahydrofuran / 20 °C / Inert atmosphere 7.1: N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 0.17 h / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

24
[ 1323290-64-7 ]
[ 2586195-79-9 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 7 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: 1-methyl-pyrrolidin-2-one; potassium carbonate / 4 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 1.5 h / -70 °C / Inert atmosphere 5.1: trifluoroacetic acid / dichloromethane / 12 h / 25 °C / Inert atmosphere 6.1: N-ethyl-N,N-diisopropylamine / tetrahydrofuran / 20 °C / Inert atmosphere 7.1: N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 0.17 h / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

25
[ 1323290-64-7 ]
[ 2586194-99-0 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 7 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: 1-methyl-pyrrolidin-2-one; potassium carbonate / 4 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 1.5 h / -70 °C / Inert atmosphere 5.1: trifluoroacetic acid / dichloromethane / 12 h / 25 °C / Inert atmosphere 6.1: N-ethyl-N,N-diisopropylamine / tetrahydrofuran / 20 °C / Inert atmosphere 7.1: N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 0.17 h / 60 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

26
[ 1323290-64-7 ]
[ 2586195-81-3 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 7 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: 1-methyl-pyrrolidin-2-one; potassium carbonate / 4 h / 25 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 1.5 h / -70 °C / Inert atmosphere 5.1: trifluoroacetic acid / dichloromethane / 12 h / 25 °C / Inert atmosphere 6.1: N-ethyl-N,N-diisopropylamine / tetrahydrofuran / 20 °C / Inert atmosphere 7.1: N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 0.17 h / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

27
[ 1323290-64-7 ]
[ 1323407-39-1 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 3 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: di-isopropyl azodicarboxylate / tetrahydrofuran / 0.5 h / 0 °C / Inert atmosphere 3.2: 1 h / 0 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

28
[ 1323290-64-7 ]
[ 1323411-23-9 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

29
[ 1323290-64-7 ]
[ 2586197-43-3 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

30
[ 1323290-64-7 ]
[ 2699881-47-3 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

31
[ 1323290-64-7 ]
[ 2586196-19-0 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

32
[ 1323290-64-7 ]
[ 2586197-46-6 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

33
[ 1323290-64-7 ]
[ 2722673-32-5 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 6 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 18 h / 40 °C / Inert atmosphere 6.1: hydrogenchloride / methanol / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

34
[ 1323290-64-7 ]
[ 2586196-22-5 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

35
[ 1323290-64-7 ]
[ 1323405-54-4 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: acetonitrile / 20 °C / Inert atmosphere 5.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 0.5 h / 0 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

36
[ 1323290-64-7 ]
[ 2586194-89-8 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: potassium carbonate / acetonitrile / 16 h / 60 °C / Inert atmosphere 4.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 2 h / -78 °C / Inert atmosphere; Cooling with acetone-dry ice 5.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 18 h / 40 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

37
[ 1323290-64-7 ]
[ CAS Unavailable ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 5 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: di-isopropyl azodicarboxylate / tetrahydrofuran / 0.5 h / 0 °C / Inert atmosphere 3.2: 1 h / 0 °C / Inert atmosphere 4.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere 5.1: potassium <i>tert</i>-butylate / tetrahydrofuran / 0.17 h / Inert atmosphere; Cooling with ice

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

38
[ 1323290-64-7 ]
[ 1323410-26-9 ]

Yield	Reaction Conditions	Operation in experiment
	Multi-step reaction with 4 steps 1.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 16 h / 60 °C / Inert atmosphere 2.1: potassium carbonate / N,N-dimethyl-formamide; water / 15 h / 15 °C / Inert atmosphere 2.2: 0.17 h / 20 °C / Inert atmosphere 3.1: di-isopropyl azodicarboxylate / tetrahydrofuran / 0.5 h / 0 °C / Inert atmosphere 3.2: 1 h / 0 °C / Inert atmosphere 4.1: N-ethyl-N,N-diisopropylamine / acetonitrile / 20 °C / Inert atmosphere

Reference： [1]Hansen, Joshua D.; Correa, Matthew; Nagy, Mark A.; Alexander, Matt; Plantevin, Veronique; Grant, Virginia; Whitefield, Brandon; Huang, Dehua; Kercher, Timothy; Harris, Roy; Narla, Rama Krishna; Leisten, Jim; Tang, Yang; Moghaddam, Mehran; Ebinger, Katalin; Piccotti, Joseph; Havens, Courtney G.; Cathers, Brian; Carmichael, James; Daniel, Thomas; Vessey, Rupert; Hamann, Lawrence G.; Leftheris, Katerina; Mendy, Derek; Baculi, Frans; Lebrun, Laurie A.; Khambatta, Gody; Lopez-Girona, Antonia [Journal of Medicinal Chemistry, 2020, vol. 63, # 13, p. 6648 - 6676]

Purity	Size	Price	VIP Price	USA Stock *0-1 Day	Global Stock *5-7 Days	Quantity
{[ item.p_purity ]}	{[ item.pr_size ]}	{[ getRatePrice(item.pr_usd, 1,1) ]} {[ getRatePrice(item.pr_usd,item.pr_rate,item.mem_rate) ]}	{[ getRatePrice(item.pr_usd, 1,1) ]}	{[ getRatePrice(item.pr_usd,item.pr_rate,item.mem_rate) ]} {[ getRatePrice(item.pr_usd,1,item.mem_rate) ]}	{[ item.pr_usastock ]}	Inquiry -	{[ item.pr_chinastock ]}	Inquiry -

CAS No. :	1323290-64-7	MDL No. :	MFCD29917025
Formula :	C₆H₁₃ClN₂O₃	Boiling Point :	-
Linear Structure Formula :	-	InChI Key :	CFJYPWQHBLMCCU-UHFFFAOYSA-N
M.W :	196.63 g/mol	Pubchem ID :	74765323
Synonyms :

Num. heavy atoms :	12
Num. arom. heavy atoms :	0
Fraction Csp3 :	0.67
Num. rotatable bonds :	5
Num. H-bond acceptors :	4.0
Num. H-bond donors :	2.0
Molar Refractivity :	44.82
TPSA :	95.41 Å²

GI absorption :	High
BBB permeant :	No
P-gp substrate :	No
CYP1A2 inhibitor :	No
CYP2C19 inhibitor :	No
CYP2C9 inhibitor :	No
CYP2D6 inhibitor :	No
CYP3A4 inhibitor :	No
Log Kp (skin permeation) :	-8.03 cm/s

Log Po/w (iLOGP) :	0.0
Log Po/w (XLOGP3) :	-0.75
Log Po/w (WLOGP) :	-0.45
Log Po/w (MLOGP) :	-0.64
Log Po/w (SILICOS-IT) :	-0.92
Consensus Log Po/w :	-0.55

[ CAS No. 1323290-64-7 ]

Quality Control of [ 1323290-64-7 ]

Related Doc. of [ 1323290-64-7 ]

Product Details of [ 1323290-64-7 ]

Calculated chemistry of [ 1323290-64-7 ]

Physicochemical Properties

Pharmacokinetics

Lipophilicity

Druglikeness

Water Solubility

Medicinal Chemistry

Safety of [ 1323290-64-7 ]

Application In Synthesis of [ 1323290-64-7 ]

[ 1323290-64-7 ] Synthesis Path-Downstream 1~38

Related Functional Groups of
[ 1323290-64-7 ]

Aliphatic Chain Hydrocarbons

Amides

Esters

Amines

Precautionary Statements-General

Prevention

Response

Storage

Disposal

Physical hazards

Health hazards

Environmental hazards

Inquiry

Lipinski :	0.0
Ghose :	None
Veber :	0.0
Egan :	0.0
Muegge :	1.0
Bioavailability Score :	0.55

Log S (ESOL) :	-0.26
Solubility :	109.0 mg/ml ; 0.554 mol/l
Class :	Very soluble
Log S (Ali) :	-0.78
Solubility :	32.9 mg/ml ; 0.167 mol/l
Class :	Very soluble
Log S (SILICOS-IT) :	-0.03
Solubility :	182.0 mg/ml ; 0.925 mol/l
Class :	Soluble

PAINS :	0.0 alert
Brenk :	0.0 alert
Leadlikeness :	1.0
Synthetic accessibility :	2.06

Signal Word:	Warning	Class:	N/A
Precautionary Statements:	P261-P305+P351+P338	UN#:	N/A
Hazard Statements:	H302-H315-H319-H335	Packing Group:	N/A
GHS Pictogram:

Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling