Type	HazMat fee for 500 gram (Estimated)
Excepted Quantity	USD 0.00
Limited Quantity	USD 15-60
Inaccessible (Haz class 6.1), Domestic	USD 80+
Inaccessible (Haz class 6.1), International	USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic	USD 100+
Accessible (Haz class 3, 4, 5 or 8), International	USD 200+

CAS No. :	1113-59-3
Formula :	C₃H₃BrO₃
M.W :	166.96
SMILES Code :	O=C(O)C(CBr)=O
Synonyms :	Bromopyruvic acid; Hexokinase II Inhibitor II, 3-BP; NSC 62343
MDL No. :	MFCD00002587
InChI Key :	PRRZDZJYSJLDBS-UHFFFAOYSA-N
Pubchem ID :	70684

GHS Pictogram:
Signal Word:	Danger
Hazard Statements:	H314
Precautionary Statements:	P280-P305+P351+P338-P310
Class:	8
UN#:	3261
Packing Group:	Ⅱ

Primary microglial cells	100 µM	24 hours	Inhibited LPS-induced NO production	J Neuroinflammation. 2021 Jun 9;18(1):129.
BV-2 microglial cells	100 µM	24 hours	Inhibited LPS-induced NO production	J Neuroinflammation. 2021 Jun 9;18(1):129.
Human erythrocytes	3.3 µM – 2 mM	1 minute	To study the kinetics of 3-BP transport in human erythrocytes. Results showed that 3-BP transport was linear during the initial 3-5 minutes and pH-dependent. The Km and Vm values were 0.89 mM and 0.94 mmol/(l cells x min), respectively. Transport was competitively inhibited by pyruvate and significantly inhibited by DIDS, SITS, CHC, and various polyphenols such as luteolin and quercetin.	Cell Mol Biol Lett. 2014 Jun;19(2):201-14.
MDA-MB-231	100 µM	1 or 24 hours	3BP was not toxic to MDA-MB-231 cells (>90% metabolic activity retained).	Cancer Metab. 2021 Oct 14;9(1):37.
BT20	100 µM	1 or 24 hours	3BP significantly reduced metabolic activity in BT20 cells (to 61% after 24-hour exposure).	Cancer Metab. 2021 Oct 14;9(1):37.
Pancreatic tumor organoids	100 µM	12 hours	To evaluate the effect of 3-BP on the metabolism of pancreatic tumor organoids, results showed significant inhibition of organoid growth and metabolic activity	Cell Death Discov. 2024 Mar 1;10(1):106.
THP-1 cells	25-100 µM	12 hours	Assessed the impact of 3-BP on THP-1 cell viability, found 25 μM 3-BP has no impact, 50 μM leads to 40% viability loss	Cells. 2020 May 8;9(5):1161.
Yeast cells	1-4 mM	1-4 hours	Assessed the impact of 3-BP on wild type cell viability, found 3 h incubation with 3 mM 3-BP is not toxic for the cells	Cells. 2020 May 8;9(5):1161.
Osteoarthritis fibroblast-like synoviocytes (OA FLS)	25 µM	24 hours	To evaluate the effect of 3-bromopyruvic acid on OA FLS proliferation, migration, and cytokine secretion. Results showed that 3-bromopyruvic acid significantly inhibited OA FLS proliferation, migration, and secretion of IL-6.	Arthritis Rheumatol. 2016 Jul;68(7):1614-26.
Rheumatoid arthritis fibroblast-like synoviocytes (RA FLS)	25 µM	24 hours	To evaluate the effect of 3-bromopyruvic acid on RA FLS proliferation, migration, and cytokine secretion. Results showed that 3-bromopyruvic acid significantly inhibited RA FLS proliferation, migration, and secretion of IL-6 and MMP-3.	Arthritis Rheumatol. 2016 Jul;68(7):1614-26.
Suit-2 cells	50–75 µM (normoxic conditions), 12.5–25 µM (hypoxic conditions)	24 hours	To evaluate the cytotoxicity of β-CD-3-BrPA and free 3-BrPA under normoxic and hypoxic conditions. Results showed that both drugs were more effective under hypoxic conditions in Suit-2 cells, though the difference was less pronounced compared to MiaPaCa-2 cells.	Clin Cancer Res. 2014 Dec 15;20(24):6406-17.
MiaPaCa-2 cells	50–75 µM (normoxic conditions), 12.5–25 µM (hypoxic conditions)	24 hours	To evaluate the cytotoxicity of β-CD-3-BrPA and free 3-BrPA under normoxic and hypoxic conditions. Results showed that both drugs were more effective under hypoxic conditions in MiaPaCa-2 cells.	Clin Cancer Res. 2014 Dec 15;20(24):6406-17.
SiMCT1-BT20	200 µM	24 hours	SiMCT1-BT20 cells showed resistance to 3BP (72% viability vs 12% in wild type).	Cancer Metab. 2021 Oct 14;9(1):37.
BT549	100 µM	24 hours	3BP rendered BT549 cells completely metabolically inactive.	Cancer Metab. 2021 Oct 14;9(1):37.
SKOV3 ovarian cancer cells	40.5 µM (IC50)	24 hours	Evaluate the cytotoxicity of 3-BP on SKOV3 cells, results showed IC50 of 40.5 μM	Int J Mol Sci. 2021 Jan 12;22(2):709.
PEO1 ovarian cancer cells	18.7 µM (IC50)	24 hours	Evaluate the cytotoxicity of 3-BP on PEO1 cells, results showed IC50 of 18.7 μM	Int J Mol Sci. 2021 Jan 12;22(2):709.
KBM7 cells	50 µM	3 days	To determine the effect of MCT1 loss on 3-BrPA sensitivity, results showed MCT1-null cells were completely resistant to 3-BrPA.	Nat Genet. 2013 Jan;45(1):104-8.
SKOV-3 cells	10 µM–100 µM	3, 6, 24 hours	Evaluate the cytotoxicity and HK2 inhibition effect of 3-BPA on SKOV-3 cells. Results showed that liposomal formulations exhibited stronger inhibitory effects and cytotoxicity at 3, 6, and 24 hours post-administration.	Int J Nanomedicine. 2015 Jul 8;10:4405-23.
Breast cancer stem cells	5–40 μg/ml	4 hours	Evaluate the metabolic inhibition and photothermal therapy effect of 3BP on breast cancer stem cells, showing significant reduction in cell viability	Adv Healthc Mater. 2022 Apr;11(8):e2102272.
F98 rodent glioma cells	0.0001 mM–0.3 mM	48 and 72 hours	3-BrPA inhibited the growth and proliferation of F98 cells with IC50 values of 15.8–25.5 μM (48 hours) and 22.3–25.0 μM (72 hours).	Neuro Oncol. 2015 Jan;17(1):70-80.
9L rodent gliosarcoma cells	0.0001 mM–0.3 mM	48 and 72 hours	3-BrPA inhibited the growth and proliferation of 9L cells with IC50 values of 15.8–25.5 μM (48 hours) and 22.3–25.0 μM (72 hours).	Neuro Oncol. 2015 Jan;17(1):70-80.
U87 human glioblastoma cells	0.0001 mM–0.3 mM	48 and 72 hours	3-BrPA inhibited the growth and proliferation of U87 cells with IC50 values of 15.8–25.5 μM (48 hours) and 22.3–25.0 μM (72 hours).	Neuro Oncol. 2015 Jan;17(1):70-80.
RAW264.7 macrophages	200 μg/mL	48 hours	Evaluate the effect of MPB-3BP@CM NPs on macrophage polarization, results showed that MPB-3BP@CM NPs promoted macrophage polarization towards M1 phenotype	Signal Transduct Target Ther. 2024 Jun 12;9(1):158.
Panc-2 cells	20 µM and 40 µM		Significant decrease in HK2 expression level	Adv Healthc Mater. 2023 Dec;12(31):e2301815.
HT29 cells	200 μg/mL	6 hours	Evaluate the antitumor efficacy of MPB-3BP@CM NPs on HT29 cells, results showed that MPB-3BP@CM NPs significantly inhibited tumor cell proliferation and induced apoptosis	Signal Transduct Target Ther. 2024 Jun 12;9(1):158.
HCT116 cells	200 μg/mL	6 hours	Evaluate the antitumor efficacy of MPB-3BP@CM NPs on HCT116 cells, results showed that MPB-3BP@CM NPs significantly inhibited tumor cell proliferation and induced apoptosis	Signal Transduct Target Ther. 2024 Jun 12;9(1):158.

F344 rats	9L gliosarcoma allograft model	Intracranial implantation	1%, 5%, 10%, 25%, or 50%	Single implantation	To evaluate the efficacy of 5% 3-BrPA polymer wafer in the 9L gliosarcoma model. Results showed that 5% 3-BrPA wafer significantly increased survival (median survival 18 days, P=0.0027).	Neuro Oncol. 2015 Jan;17(1):70-80.
C57BL/6 female mice	Subcutaneous pancreatic cancer model	Injection	10 mg/kg	Three times per week for 30 days	Tumor volume reduced by 60-80%, decreased HK2 expression, increased Caspase3 expression	Adv Healthc Mater. 2023 Dec;12(31):e2301815.
Balb/c nude mice	4T1 and MDA-MB-231 tumor models	Intravenous injection	10 mg/kg	Single injection followed by photothermal therapy after 24 hours	Evaluate the metabolic inhibition and photothermal therapy effect of 3BP in vivo, showing significant tumor growth inhibition and reduced expression of CSC markers	Adv Healthc Mater. 2022 Apr;11(8):e2102272.
Balb/c nude mice	4T1 tumor model	Intravenous injection	10 mg/mL	Single injection, observed for 48 hours	Evaluate the in vivo distribution and therapeutic effects of 3BP@PLGA-IR780, showing effective accumulation in tumor sites and inhibition of tumor growth.	J Nanobiotechnology. 2021 Dec 20;19(1):440
BALB/c nude mice	HCT116 subcutaneous tumor model	Intravenous injection	30.0 mg/kg	Every other day for a total of three doses	Evaluate the antitumor efficacy of MPB-3BP@CM NPs in vivo, results showed that MPB-3BP@CM NPs significantly inhibited tumor growth and prolonged survival time	Signal Transduct Target Ther. 2024 Jun 12;9(1):158.
Mice	K/BxN serum transfer arthritis model	Intraperitoneal injection	5 mg/kg	Once daily for 6 days	To evaluate the effect of 3-bromopyruvic acid on arthritis severity. Results showed that 3-bromopyruvic acid significantly reduced clinical and histopathological scores of arthritis, decreased joint inflammation and cartilage damage.	Arthritis Rheumatol. 2016 Jul;68(7):1614-26.
Male athymic nude mice	Orthotopic xenograft model of human pancreatic ductal adenocarcinoma (PDAC)	Intraperitoneal injection	5 mg/kg	Daily for 4 weeks	To evaluate the antitumor efficacy and toxicity of β-CD-3-BrPA in vivo. Results showed that β-CD-3-BrPA significantly inhibited tumor growth without toxicity, while free 3-BrPA exhibited high toxicity.	Clin Cancer Res. 2014 Dec 15;20(24):6406-17.
Nude mice	Subcutaneous xenograft model		8 mg/kg	3 weeks	To evaluate the effect of MCT1 expression on tumor sensitivity to 3-BrPA, results showed MCT1 expression enhanced tumor sensitivity to 3-BrPA.	Nat Genet. 2013 Jan;45(1):104-8.

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CAS No.: 1113-59-3

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CAS No.: 1113-59-3

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Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling

Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
H224	Extremely flammable liquid and vapour
H225	Highly flammable liquid and vapour
H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed