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Type HazMat fee for 500 gram (Estimated)
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Chemical Structure| 6775-01-5 Chemical Structure| 6775-01-5

Structure of 6775-01-5

Chemical Structure| 6775-01-5

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Product Citations

Product Citations

Marion H. Emmert ; Cuixian Yang ; Eugene E. Kwan ; Rebecca Chmielowski ; Bruce Kilgore ; Zachary L. VanAernum , et al.

Abstract: This manuscript describes the detailed evaluation of more than 40 phosphine reductants via automated and nonautomated high-throughput experimentation approaches with the goal of identifying selective reductants for cleaving the disulfide bonds of capped, engineered cysteines in a proprietary monoclonal antibody (mAb). As a point of reference, this study included phosphines that have previously been documented in the literature [4-diphenylphosphino benzoic acid (DPPBA), tris(3-sulfophenyl)phosphine (TSPP), and 3-(diphenylphosphino)benzenesulfonate (diPPBS)]; however, all known reductants showed the formation of undesired side products upon reduction (detectable by IEX), especially at higher phosphine loadings. The high_x005f_x0002_throughput study also revealed several phosphines with potential for selective reduction that had not been previously studied for this type of transformation. These initial hits were further evaluated with regard to the phosphine/mAb ratio, solubility in aqueous media, and air oxidation behavior. The best phosphine identified (1-(4-(diphenylphosphanyl)benzyl)-1-methylpyrrolidin-1-ium bromide (P10)) was then employed in a sequence of high-throughput studies that established efficient one-pot reduction/conjugation reaction conditions. Overall, the work detailed herein demonstrates how high-throughput experimental design enables rapid and resource-sparing insights into mAb reduction and conjugation reactivity with phosphine-based reductants.

Keywords: high-throughput experimentation (HTE) ; monoclonal antibody (mAb) ; disulfide bond reduction ; mAb decapping ; phosphines ; one-pot reduction/conjugation

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Product Details of [ 6775-01-5 ]

CAS No. :6775-01-5
Formula : C12H21N2P
M.W : 224.28
SMILES Code : NCCCP(CCCN)C1=CC=CC=C1
MDL No. :MFCD00014834

Safety of [ 6775-01-5 ]

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H302-H314
Precautionary Statements:P264-P270-P271-P280-P303+P361+P353-P304+P340-P305+P351+P338-P310-P330-P331-P363-P403+P233-P501
Class:8
UN#:3267
Packing Group:
 

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