[ CAS No. 69-93-2 ] {[proInfo.proName]}

Name: 69-93-2|1H-Purine-2,6,8(3H,7H,9H)-trione|98%
Brand: Ambeed
SKU: A744554
Rating: 93 (26 reviews)

,{[proInfo.pro_purity]}

Cat. No.: {[proInfo.prAm]}

2D 3D

Structure of 69-93-2 * Storage: {[proInfo.prStorage]}

Cart0 Add to My Favorites Add to My Favorites Bulk Inquiry Inquiry Add To Cart

Search after Editing

* Storage: {[proInfo.prStorage]}

For Research Only 110K+ Compounds Competitive Price 1-2 Day Shipping

Quality Control of [ 69-93-2 ]

COA NMR CNMR HPLC LC-MS

Related Doc. of [ 69-93-2 ]

SDS Specification

Alternatived Products of [ 69-93-2 ]

Product Details of [ 69-93-2 ]

CAS No. :	69-93-2	MDL No. :	MFCD00005712
Formula :	C₅H₄N₄O₃	Boiling Point :	-
Linear Structure Formula :	-	InChI Key :	LEHOTFFKMJEONL-UHFFFAOYSA-N
M.W :	168.11	Pubchem ID :	1175
Synonyms :		Chemical Name :	1H-Purine-2,6,8(3H,7H,9H)-trione

Calculated chemistry of [ 69-93-2 ]

Physicochemical Properties

Num. heavy atoms :	12
Num. arom. heavy atoms :	9
Fraction Csp3 :	0.0
Num. rotatable bonds :	0
Num. H-bond acceptors :	3.0
Num. H-bond donors :	4.0
Molar Refractivity :	40.16
TPSA :	114.37 Å²

Pharmacokinetics

GI absorption :	High
BBB permeant :	No
P-gp substrate :	No
CYP1A2 inhibitor :	No
CYP2C19 inhibitor :	No
CYP2C9 inhibitor :	No
CYP2D6 inhibitor :	No
CYP3A4 inhibitor :	No
Log Kp (skin permeation) :	-8.68 cm/s

Lipophilicity

Log Po/w (iLOGP) :	-0.2
Log Po/w (XLOGP3) :	-1.91
Log Po/w (WLOGP) :	-1.77
Log Po/w (MLOGP) :	-1.32
Log Po/w (SILICOS-IT) :	1.61
Consensus Log Po/w :	-0.72

Druglikeness

Lipinski :	0.0
Ghose :	None
Veber :	0.0
Egan :	0.0
Muegge :	1.0
Bioavailability Score :	0.55

Water Solubility

Log S (ESOL) :	-0.23
Solubility :	98.1 mg/ml ; 0.583 mol/l
Class :	Very soluble
Log S (Ali) :	0.03
Solubility :	180.0 mg/ml ; 1.07 mol/l
Class :	Highly soluble
Log S (SILICOS-IT) :	-1.88
Solubility :	2.21 mg/ml ; 0.0132 mol/l
Class :	Soluble

Medicinal Chemistry

PAINS :	0.0 alert
Brenk :	0.0 alert
Leadlikeness :	1.0
Synthetic accessibility :	1.8

Safety of [ 69-93-2 ]

Signal Word:	Warning	Class:	N/A
Precautionary Statements:	P261-P280-P305+P351+P338	UN#:	N/A
Hazard Statements:	H302-H315-H319-H332-H335	Packing Group:	N/A
GHS Pictogram:

Application In Synthesis of [ 69-93-2 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

Upstream synthesis route of [ 69-93-2 ]
Downstream synthetic route of [ 69-93-2 ]

[ 69-93-2 ] Synthesis Path-Upstream 1~2

1
[ 69-93-2 ]
[ 873-83-6 ]

Reference： [1] Nucleosides, Nucleotides and Nucleic Acids, 2008, vol. 27, # 8, p. 967 - 978

2
[ 69-93-2 ]
[ 87-39-8 ]

Reference： [1] Monatshefte fuer Chemie, 1900, vol. 21, p. 283

[ 69-93-2 ] Synthesis Path-Downstream 1~88

1
[ 186581-53-3 ]
[ 69-93-2 ]
[ 2309-49-1 ]

Reference： [1]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1921,vol. 117,p. 18

2
[ 2525-16-8 ]
[ 69-93-2 ]
[ 2309-49-1 ]

Reference： [1]Patent: DE944312,1953,

3
[ 3240-72-0 ]
[ 57-13-6 ]
[ 69-93-2 ]

Reference： [1]Journal of Biological Chemistry,1916,vol. 25,p. 618

4
[ 69-93-2 ]
[ 627-45-2 ]
[ 69-89-6 ]

Reference： [1]Patent: DE836803,1949,
DRP/DRBP Org.Chem.,

5
[ 69-93-2 ]
[ 544-17-2 ]
[ 69-89-6 ]

Reference： [1]Skandinavisches Archiv fur Physiologie,vol. 25,p. 256
Chemisches Zentralblatt,1911,vol. 82,p. 1411

6
[ 69-93-2 ]
[ 144-62-7 ]
[ 56-81-5 ]
[ 645-93-2 ]
[ 69-89-6 ]

Reference： [1]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1911,vol. 76,p. 487

7
[ 69-93-2 ]
[ 77287-34-4 ]
[ 68-94-0 ]
[ 69-89-6 ]

Reference： [1]Patent: DE804210,1950,
DRP/DRBP Org.Chem.,

8
[ 69-93-2 ]
[ 77287-34-4 ]
[ 69-89-6 ]

Reference： [1]Chemische Berichte,1950,vol. 83,p. 201,209
[2]Chemische Berichte,1950,vol. 83,p. 201,209
[3]Patent: DE804210,1950,
DRP/DRBP Org.Chem.,

9
[ 64-18-6 ]
[ 69-93-2 ]
[ 56-81-5 ]
[ 69-89-6 ]

Reference： [1]Journal fur praktische Chemie (Leipzig 1954),vol. <2>118,p. 168

10
[ 69-93-2 ]
[ 103-70-8 ]
[ 69-89-6 ]

Reference： [1]Patent: DE836803,1949,
DRP/DRBP Org.Chem.,

11
[ 69-93-2 ]
[ 74-88-4 ]
[ 2309-49-1 ]

Yield	Reaction Conditions	Operation in experiment
69.2%	at 84℃; under 3750.38 Torr; for 2.0h;Inert atmosphere; Autoclave;	step one, to 500 ml high-pressure reaction kettle adding 1.6mol iodo methane and 0.08mol urate, after the airtight to the high-pressure reaction in cauldron sufficient nitrogen gas to 0.5 MPa after emptying, continuous replacement after three nitrogen in cauldron sufficient to the high-pressure reaction to the high-pressure reaction kettle pressure is 5 MPa, condensed water is opened, adjusting the stirring rate for 180rpm, the temperature of the high-pressure reactor to 84 C, thermal insulation reaction 2h; Step two, the step of condensing water flow is increased in a high-pressure reaction kettle of the insulation after the reaction temperature, high-pressure reaction kettle and slowly opening the air outlet valve to relieve pressure, to be high-pressure reactor pressure is dropped to 0 MPa operates the cauldron the discharge, of the material after the reaction is poured into 300 ml ice water to stirring crystallization, crude product obtained after filtering; Step three, ethyl acetate and methanol to the in the second step of performing recrystallization states thickly, ethyl acetate and methanol volume ratio of 5 : 1,105 C drying 0.5 hours to obtain the tetramethyl uric acid (1, 3, 7, 9-tetramethyl-urate) 12.4g, yield 69.2%, the quality of the uric acid tetramethyl-detection HPLC purity of not less than 95%.

Reference： [1]Patent: CN104086550,2016,B .Location in patent: Paragraph 0022-0025
[2]Patent: DE93112,
Fortschr. Teerfarbenfabr. Verw. Industriezweige,vol. 4,p. 1255

12
[ 69-93-2 ]
[ 97-59-6 ]

Yield	Reaction Conditions	Operation in experiment
	With sodium hexachloroiridate; at 20℃;pH 7;aq. phosphate buffer;	In a final volume of 240 muL of 75 mM NaPi buffer, AcO-RU (0.3 mM) was incubated with Na2IrCl6 (0.6 mM) orK3Fe(CN)6 (0.6 mM) at room temperature. The reaction mixture was analyzed by reversed phaseHPLC using a Varian C18 (5 mum, 250X4.6 mm) column with 5% solvent B for the first 5 minfollowed by a gradient to 65% solvent B in 30 min (solvent A: 0.1% TFA in water, solvent B:0.1% TFA in acetonitrile). The flow rate was 1 mL/min and the detector was set at 220 nm.

Reference： [1]Tetrahedron,1986,vol. 42,p. 747 - 752
[2]Biochemische Zeitschrift,1931,vol. 236,p. 136
[3]Justus Liebigs Annalen der Chemie,1859,vol. 110,p. 94
[4]Justus Liebigs Annalen der Chemie,1838,vol. 26,p. 256,260
Gm.,vol. 2,p. 308
[5]Justus Liebigs Annalen der Chemie,1848,vol. 67,p. 219
[6]Chemische Berichte,1874,vol. 7,p. 229
[7],1907,vol. 9,p. 305
A.Pth.,1909,vol. 60,p. 199
[8]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1904,vol. 41,p. 344,347
[9]Journal of the American Chemical Society,1918,vol. 40,p. 1099
[10]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1927,vol. 162,p. 192,194,203
[11]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1927,vol. 171,p. 176
[12]Biochemische Zeitschrift,1925,vol. 159,p. 135
[13]Journal fur praktische Chemie (Leipzig 1954),1923,vol. <2>106,p. 115,136
[14]Helvetica Chimica Acta,1926,vol. 9,p. 438
[15]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1927,vol. 162,p. 192,194,203
[16]Org.Synth.13<1933>,1;Coll.Vol.II<1943>,21,
[17]Zeitschrift fur Chemie,1866,p. 746
[18]Journal fur praktische Chemie (Leipzig 1954),1923,vol. <2>106,p. 115,136
[19]Canadian Journal of Chemistry,1994,vol. 72,p. 1668 - 1674
[20]Canadian Journal of Chemistry,1994,vol. 72,p. 1668 - 1674
[21]Journal of the American Chemical Society,1997,vol. 119,p. 5435 - 5442
[22]Analytical Chemistry,1999,vol. 71,p. 1928 - 1934
[23]Tetrahedron Letters,2011,vol. 52,p. 2176 - 2180
[24]Russian Journal of Applied Chemistry,2011,vol. 84,p. 218 - 224
[25]Photochemistry and Photobiology,2013,vol. 89,p. 432 - 441
[26]Dyes and Pigments,2015,vol. 121,p. 1 - 6
[27]RSC Advances,2019,vol. 9,p. 36578 - 36585

13
[ 69-93-2 ]
[ 120-89-8 ]

Reference： [1]Justus Liebigs Annalen der Chemie,1918,vol. 416,p. 226
[2]Justus Liebigs Annalen der Chemie,1848,vol. 68,p. 24
Annales de Chimie (Cachan, France),1848,vol. <3> 24,p. 176
[3]Justus Liebigs Annalen der Chemie,1874,vol. 172,p. 79
[4]Bulletin de la Societe Chimique de France,1874,vol. <2> 22,p. 56
[5]Zeitschrift fur Chemie,1866,p. 746
[6]Justus Liebigs Annalen der Chemie,1873,vol. 166,p. 321
[7]Journal fur praktische Chemie (Leipzig 1954),1923,vol. <2>106,p. 115,136
[8]Photochemistry and Photobiology,2019,vol. 95,p. 202 - 210

14
[ 69-93-2 ]
[ 108-80-5 ]

Yield	Reaction Conditions	Operation in experiment
50%	With dihydrogen peroxide In not given oxidation of uric acid by alk. H2O2-soln.;
	With sodium hydroxide; dihydrogen peroxide at 20℃; rascher in der Waerme;
	heating;

With dihydrogen peroxide In not given oxidation of uric acid by alk. H2O2-soln.;

Reference： [1]Venable, C. S.; Moore, F. J. [Journal of the American Chemical Society, 1917, vol. 39, p. 1750 - 1755] [Gmelin Handbuch der Anorganischen Chemie, Gmelin Handbook: C: MVol.D1, 39.1.4, page 368 - 368]
[2]Kodweiss [Annalen der Physik, 1830, vol. 19, p. 12] Woehler [Annalen der Physik, 1829, vol. 15, p. 622][Annales de Chimie (Cachan, France), 1830, vol. <2>43, p. 67] Chevallier; Lassaigne [Annales de Chimie (Cachan, France), 1820, vol. <2>13, p. 155]
[3]Venable [Journal of the American Chemical Society, 1918, vol. 40, p. 1099] Walters; Wise [Journal of the American Chemical Society, 1917, vol. 39, p. 2472] Venable; Moore [Journal of the American Chemical Society, 1917, vol. 39, p. 1750] Scholtz [Chemische Berichte, 1901, vol. 34, p. 4130]
[4]Chevallier, A.; Lassaigne, J.-L. [Annales de Chimie et de Physique, 1820, vol. 13, p. 155 - 162] Woehler, F. [Annalen der Physik, 1829, vol. 15, p. 619 - 630] [Gmelin Handbuch der Anorganischen Chemie, Gmelin Handbook: C: MVol.D1, 39.1, page 366 - 366] Scheele, C. W. [in: S. F. Hermbstaedt, Saemtliche physische und chemische Werke, Bd. 2, 1793, S. 149]
[5]Hartman; Fellig [Journal of the American Chemical Society, 1955, vol. 77, p. 1051] Brandenberger, H. [Helvetica Chimica Acta, 1954, vol. 37, p. 641 - 644] [Gmelin Handbuch der Anorganischen Chemie, Gmelin Handbook: C: MVol.D1, 39.1.4, page 368 - 368]
[6]Scheele,C.W. [Saemtliche Physische und Chemische Werke, herausgegeben von S.Fr.Hermbstaedt,Bd.II<Berlin 1793>,S.149]

16
[ 69-93-2 ]
[ 645-93-2 ]
[ 69-89-6 ]

Reference： [1]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1911,vol. 76,p. 487

17
[ 68-94-0 ]
[ 69-93-2 ]

Yield	Reaction Conditions	Operation in experiment
	With ethylenediaminetetraacetic acid; (E)-2-(3-methyl-2-buten-1-yl)-3,4',5-trihydroxystilbene; sodium 3,3'-<1-<(phenylamino)carbonyl>-3,4-tetrazolium>-bis(4-methoxy-6-nitro)benzenesulfonic acid;xanthine oxidase; In water;pH 9.4;Sodium carbonate buffer;Reactivity;	Scavenging of superoxide anion radicals formed enzymatically by xanthine oxidase (X/XO) Superoxide anion radicals were generated by oxidation of hypoxanthine to uric acid by xanthine oxidase and quantitated by the concomitant reduction of XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)- 2H-tetrazolium-5-carboxanilide] (modified from Ukeda et a/., 1997 and adjusted to a 96-well microplate format). The reaction mixture (170 mul), consisting of 50 mM sodium carbonate buffer, pH 9.4, EDTA (100 muM final concentration), NBT (12.5 muM final concentration) and hypoxanthine (50 muM final concentration), was mixed with test compounds (10 mul, in 100% DMSO, 5% final DMSO concentration) or DMSO (10 mul) as a solvent control. 1 U SOD (in 10 mul 50 mM sodium carbonate buffer, pH 9.4) was used as a positive control. The reaction was started by addition of 3 mil xanthine oxidase (in 10 mul buffer), and the rate of reduction of XTT was monitored for 6 min at 480 nm in a microplate reader (Spectramax 340, Molecular Devices). Vmax values were computed, and the halfmaximal scavenging concentration SC50 was generated from the data obtained with 8 serial two- <n="15"/>fold dilutions of inhibitors in a final concentration range of 2 - 250 muM tested in duplicates. To exclude a direct inhibitory effect on xanthine oxidase, formation of uric acid was monitored directly at 290 nm under identical conditions as described above without addition of XTT. In the reaction mixture, 50 muM hypoxanthine was replaced by 100 muM xanthine.
	With ethylenediaminetetraacetic acid; (E)-5-[2-4-(hydroxyphenyl)ethenyl]-1,3-benzenediol; sodium 3,3'-<1-<(phenylamino)carbonyl>-3,4-tetrazolium>-bis(4-methoxy-6-nitro)benzenesulfonic acid;xanthine oxidase; In water;pH 9.4;Sodium carbonate buffer;Reactivity;	Scavenging of superoxide anion radicals formed enzymatically by xanthine oxidase (X/XO) Superoxide anion radicals were generated by oxidation of hypoxanthine to uric acid by xanthine oxidase and quantitated by the concomitant reduction of XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)- 2H-tetrazolium-5-carboxanilide] (modified from Ukeda et a/., 1997 and adjusted to a 96-well microplate format). The reaction mixture (170 mul), consisting of 50 mM sodium carbonate buffer, pH 9.4, EDTA (100 muM final concentration), NBT (12.5 muM final concentration) and hypoxanthine (50 muM final concentration), was mixed with test compounds (10 mul, in 100% DMSO, 5% final DMSO concentration) or DMSO (10 mul) as a solvent control. 1 U SOD (in 10 mul 50 mM sodium carbonate buffer, pH 9.4) was used as a positive control. The reaction was started by addition of 3 mil xanthine oxidase (in 10 mul buffer), and the rate of reduction of XTT was monitored for 6 min at 480 nm in a microplate reader (Spectramax 340, Molecular Devices). Vmax values were computed, and the halfmaximal scavenging concentration SC50 was generated from the data obtained with 8 serial two- <n="15"/>fold dilutions of inhibitors in a final concentration range of 2 - 250 muM tested in duplicates. To exclude a direct inhibitory effect on xanthine oxidase, formation of uric acid was monitored directly at 290 nm under identical conditions as described above without addition of XTT. In the reaction mixture, 50 muM hypoxanthine was replaced by 100 muM xanthine.
	With ethylenediaminetetraacetic acid; 2,6-diprenyl-3,5,4'-trihydroxystilbene; nitroblue tetrazolium chloride; sodium 3,3'-<1-<(phenylamino)carbonyl>-3,4-tetrazolium>-bis(4-methoxy-6-nitro)benzenesulfonic acid;xanthine oxidase; In water; dimethyl sulfoxide;pH 9.4;Sodium carbonate buffer;Reactivity;	Scavenging of superoxide anion radicals formed enzymatically by xanthine oxidase (X/XO) Superoxide anion radicals were generated by oxidation of hypoxanthine to uric acid by xanthine oxidase and quantitated by the concomitant reduction of XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)- 2H-tetrazolium-5-carboxanilide] (modified from Ukeda et a/., 1997 and adjusted to a 96-well microplate format). The reaction mixture (170 mul), consisting of 50 mM sodium carbonate buffer, pH 9.4, EDTA (100 muM final concentration), NBT (12.5 muM final concentration) and hypoxanthine (50 muM final concentration), was mixed with test compounds (10 mul, in 100% DMSO, 5% final DMSO concentration) or DMSO (10 mul) as a solvent control. 1 U SOD (in 10 mul 50 mM sodium carbonate buffer, pH 9.4) was used as a positive control. The reaction was started by addition of 3 mil xanthine oxidase (in 10 mul buffer), and the rate of reduction of XTT was monitored for 6 min at 480 nm in a microplate reader (Spectramax 340, Molecular Devices). Vmax values were computed, and the halfmaximal scavenging concentration SC50 was generated from the data obtained with 8 serial two- <n="15"/>fold dilutions of inhibitors in a final concentration range of 2 - 250 muM tested in duplicates. To exclude a direct inhibitory effect on xanthine oxidase, formation of uric acid was monitored directly at 290 nm under identical conditions as described above without addition of XTT. In the reaction mixture, 50 muM hypoxanthine was replaced by 100 muM xanthine.

	With ethylenediaminetetraacetic acid; sodium 3,3'-<1-<(phenylamino)carbonyl>-3,4-tetrazolium>-bis(4-methoxy-6-nitro)benzenesulfonic acid;superoxide dismutase; xanthine oxidase; In water;pH 9.4;Sodium carbonate buffer;Reactivity;	Scavenging of superoxide anion radicals formed enzymatically by xanthine oxidase (X/XO) Superoxide anion radicals were generated by oxidation of hypoxanthine to uric acid by xanthine oxidase and quantitated by the concomitant reduction of XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)- 2H-tetrazolium-5-carboxanilide] (modified from Ukeda et a/., 1997 and adjusted to a 96-well microplate format). The reaction mixture (170 mul), consisting of 50 mM sodium carbonate buffer, pH 9.4, EDTA (100 muM final concentration), NBT (12.5 muM final concentration) and hypoxanthine (50 muM final concentration), was mixed with test compounds (10 mul, in 100% DMSO, 5% final DMSO concentration) or DMSO (10 mul) as a solvent control. 1 U SOD (in 10 mul 50 mM sodium carbonate buffer, pH 9.4) was used as a positive control. The reaction was started by addition of 3 mil xanthine oxidase (in 10 mul buffer), and the rate of reduction of XTT was monitored for 6 min at 480 nm in a microplate reader (Spectramax 340, Molecular Devices). Vmax values were computed, and the halfmaximal scavenging concentration SC50 was generated from the data obtained with 8 serial two- <n="15"/>fold dilutions of inhibitors in a final concentration range of 2 - 250 muM tested in duplicates. To exclude a direct inhibitory effect on xanthine oxidase, formation of uric acid was monitored directly at 290 nm under identical conditions as described above without addition of XTT. In the reaction mixture, 50 muM hypoxanthine was replaced by 100 muM xanthine.
	With ethylenediaminetetraacetic acid; C24H28O3; sodium 3,3'-<1-<(phenylamino)carbonyl>-3,4-tetrazolium>-bis(4-methoxy-6-nitro)benzenesulfonic acid;xanthine oxidase; In water;pH 9.4;Sodium carbonate buffer;Reactivity;	Scavenging of superoxide anion radicals formed enzymatically by xanthine oxidase (X/XO) Superoxide anion radicals were generated by oxidation of hypoxanthine to uric acid by xanthine oxidase and quantitated by the concomitant reduction of XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)- 2H-tetrazolium-5-carboxanilide] (modified from Ukeda et a/., 1997 and adjusted to a 96-well microplate format). The reaction mixture (170 mul), consisting of 50 mM sodium carbonate buffer, pH 9.4, EDTA (100 muM final concentration), NBT (12.5 muM final concentration) and hypoxanthine (50 muM final concentration), was mixed with test compounds (10 mul, in 100% DMSO, 5% final DMSO concentration) or DMSO (10 mul) as a solvent control. 1 U SOD (in 10 mul 50 mM sodium carbonate buffer, pH 9.4) was used as a positive control. The reaction was started by addition of 3 mil xanthine oxidase (in 10 mul buffer), and the rate of reduction of XTT was monitored for 6 min at 480 nm in a microplate reader (Spectramax 340, Molecular Devices). Vmax values were computed, and the halfmaximal scavenging concentration SC50 was generated from the data obtained with 8 serial two- <n="15"/>fold dilutions of inhibitors in a final concentration range of 2 - 250 muM tested in duplicates. To exclude a direct inhibitory effect on xanthine oxidase, formation of uric acid was monitored directly at 290 nm under identical conditions as described above without addition of XTT. In the reaction mixture, 50 muM hypoxanthine was replaced by 100 muM xanthine.

Reference： [1]Journal of Organic Chemistry,1980,vol. 45,p. 3072 - 3077
[2]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1914,vol. 92,p. 439
[3]94. 111,113,117,120,
 Chemisches Zentralblatt,1922,vol. 93,p. 1360
[4]94. 111,113,117,120,
 Chemisches Zentralblatt,1922,vol. 93,p. 1360
[5]94. 111,113,117,120,
 Chemisches Zentralblatt,1922,vol. 93,p. 1360
[6]94. 111,113,117,120,
 Chemisches Zentralblatt,1922,vol. 93,p. 1360
[7]Justus Liebigs Annalen der Chemie,1930,vol. 477,p. 77
[8]Patent: WO2009/12910,2009,A1 .Location in patent: Page/Page column 13-14; 19
[9]Patent: WO2009/12910,2009,A1 .Location in patent: Page/Page column 13-14; 19
[10]Patent: WO2009/12910,2009,A1 .Location in patent: Page/Page column 13-14; 19
[11]Patent: WO2009/12910,2009,A1 .Location in patent: Page/Page column 13-14; 19
[12]Patent: WO2009/12910,2009,A1 .Location in patent: Page/Page column 13-14; 19
[13]Analytical Chemistry,2012,vol. 84,p. 10359 - 10365

18
[ 68-94-0 ]
[ 69-93-2 ]
[ 69-89-6 ]

Reference： [1]Lehrb. d. physiol. Chemie, 5. Aufl., I. Teil, S. 677,

19
[ 42965-55-9 ]
[ 57-13-6 ]
[ 69-93-2 ]

Reference： [1]Zhurnal Prikladnoi Khimii,1946,vol. 19,p. 1340
Chem.Abstr.,1948,p. 1207

20
[ 69-89-6 ]
[ 69-93-2 ]

Yield	Reaction Conditions	Operation in experiment
	With diethylenetriaminopentaacetic acid; C39H37O4S12(2-)*2Na(1+); xanthine oxidase;	The O2?- generation rates at various O2?- fluxes from the X/XO system were assessed by EPR spectroscopy using CT02-H with measurement of its signal decay. These results were compared to values measured by UV-Vis spectroscopy using cytochrome c. UV-Vis spectra were recorded at room temperature on a Varian Cary 50 Bio spectrophotometer. First, the enzymatic activity of the commercial XO was determined by monitoring the production of uric acid (12.2 mM-1 cm-1 at 290 nm) in the presence of excess X. Then, various concentrations of XO (0.1, 0.2, 0.5, 1, 2, and 5 mU/ml) were added to the solution containing X (100 muM), DTPA (0.1 mM), and CT02-H (50 muM), with the O2?- generation rate determined from the decay rate of the EPR signal of CT02-H. For the measurements of the O2?- generation rate from the reduction of ferricytochrome c, various concentrations of XO (2, 5, 10, and 20 mU/ml) were added to solutions containing X (100 muM), EDTA (100 muM), catalase (200 U/ml), and ferricytochrome c (100 muM). The O2?- generation rate was determined from the reduction of ferricytochrome c to ferrocytochrome c by O2?-. It was measured from the slope of the time-dependent production of ferrocytochrome c measured at 550 nm using an extinction coefficient of 2.1×104 M-1 cm-1 [42,43].
	With hydroxylamine; xanthine oxidase; In aq. phosphate buffer; at 37℃; for 0.5h;pH 7.5;Enzymatic reaction;	The enzyme <strong>[69-89-6]xanthin</strong>e oxidase catalyzes the oxidation of <strong>[69-89-6]xanthin</strong>e to uric acid. Inhibition of<strong>[69-89-6]xanthin</strong>e oxidase results in a decreased production of uric acid. The uric acid production was measuredaccording to the increasing absorbance at 290 nm. Test solutions were prepared by adding <strong>[69-89-6]xanthin</strong>e(final concentrations 50 M), hydroxylamine (final concen-tration 0.2 mM), EDTA (final concentration0.1 mM), and flavonol glycosides in four concentrations (2.5, 5.0, 10.0, 20.0 M). The reaction wasstarted by adding 0.2 mL of <strong>[69-89-6]xanthin</strong>e oxidase (6.25 mU/mL) in a phosphate buffer solution (pH = 7.50,200 mM). The mixture (total 1 mL) was incubated for 30 min at 37 C. Prior to the measurement of uricacid prodution, the reaction was stopped by adding 0.1 mL of HCl (0.58 M) [22]. The IC50 values werecalculated by the mean data values from three determinations.

Reference： [1]Chemical and Pharmaceutical Bulletin,1981,vol. 29,p. 430 - 432
[2]94. 111,113,117,120,
 Chemisches Zentralblatt,1922,vol. 93,p. 1360
[3]94. 111,113,117,120,
 Chemisches Zentralblatt,1922,vol. 93,p. 1360
[4]94. 111,113,117,120,
 Chemisches Zentralblatt,1922,vol. 93,p. 1360
[5]94. 111,113,117,120,
 Chemisches Zentralblatt,1922,vol. 93,p. 1360
[6]Justus Liebigs Annalen der Chemie,1930,vol. 477,p. 77
[7]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1914,vol. 92,p. 439
[8]Bioscience, Biotechnology and Biochemistry,1999,vol. 63,p. 1787 - 1790
[9]Analytical Chemistry,2006,vol. 78,p. 530 - 537
[10]Bioscience, Biotechnology and Biochemistry,2008,vol. 72,p. 2158 - 2163
[11]Journal of Natural Products,2009,vol. 72,p. 725 - 731
[12]Free Radical Biology and Medicine,2010,vol. 48,p. 493 - 498
[13]Analytical Chemistry,2012,vol. 84,p. 10359 - 10365
[14]Free Radical Biology and Medicine,2012,vol. 53,p. 2081 - 2091
[15]RSC Advances,2015,vol. 5,p. 32183 - 32190
[16]Chemistry - A European Journal,2017,vol. 23,p. 6983 - 6987
[17]Electrochimica Acta,2017,vol. 237,p. 44 - 53
[18]Molecules,2018,vol. 23
[19]RSC Advances,2018,vol. 8,p. 10023 - 10031
[20]Analytical Chemistry,2019,vol. 91,p. 12768 - 12774

21
[ 73-40-5 ]
[ 69-93-2 ]
[ 69-89-6 ]

Reference： [1]Biochemische Zeitschrift,1912,vol. 47,p. 222

22
[ 69-93-2 ]
[ 120-89-8 ]
[ 61066-33-9 ]
[ 36597-25-8 ]
[ 3237-50-1 ]
[ 57-13-6 ]
[ 97-59-6 ]

Reference： [1]Analytical chemistry,1989,vol. 61,p. 1709 - 1717

23
[ 69-93-2 ]
[ 120-89-8 ]
[ 5676-27-7 ]
[ 105245-87-2 ]
[ 97-59-6 ]

Reference： [1]Tetrahedron,1986,vol. 42,p. 747 - 752
[2]Tetrahedron,1986,vol. 42,p. 747 - 752

24
[ 69-93-2 ]
[ 61066-33-9 ]
[ 57-13-6 ]
[ 97-59-6 ]

Reference： [1]Indian Journal of Chemistry, Section A: Inorganic, Physical, Theoretical and Analytical,1989,vol. 28,p. 467 - 471
[2]Indian Journal of Chemistry, Section A: Inorganic, Physical, Theoretical and Analytical,1997,vol. 36,p. 283 - 289

25
[ 69-93-2 ]
[ 508-37-2 ]
[ 97-59-6 ]

Reference： [1]Tetrahedron,1988,vol. 44,p. 6723 - 6728

26
[ 69-93-2 ]
[ 121669-46-3 ]
[ 97-59-6 ]

Reference： [1]Tetrahedron,1988,vol. 44,p. 6723 - 6728

27
[ 69-89-6 ]
[ 61066-33-9 ]
[ 69-93-2 ]
[ 57-13-6 ]

Reference： [1]Indian Journal of Chemistry, Section A: Inorganic, Physical, Theoretical and Analytical,1989,vol. 28,p. 467 - 471

28
[ 69-93-2 ]
[ 7722-84-1 ]
furan-2,3,5(4H)-trione pyridine (1:1) [ No CAS ]
[ 97-59-6 ]

Reference： [1]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,vol. 162,p. 222

29
[ 69-93-2 ]
air oxygen [ No CAS ]
alkaline solution [ No CAS ]
[ 97-59-6 ]

Reference： [1]Journal of Biochemistry,vol. 9,p. 163
Chemisches Zentralblatt,1929,vol. 100,p. 1169

30
[ 69-93-2 ]
[ 7553-56-2 ]
KOH-solution [ No CAS ]
[ 97-59-6 ]

Reference： [1]Monatshefte fur Chemie,vol. 15,p. 528

31
[ 69-93-2 ]
lithium carbonate [ No CAS ]
lead dioxide [ No CAS ]
[ 97-59-6 ]

Reference： [1]Helvetica Chimica Acta,vol. 9,p. 429,430

32
[ 69-93-2 ]
lithium carbonate [ No CAS ]
nickel [ No CAS ]
[ 97-59-6 ]

Reference： [1]Helvetica Chimica Acta,vol. 9,p. 429,430

33
[ 69-93-2 ]
lithium carbonate [ No CAS ]
platinum [ No CAS ]
[ 97-59-6 ]

Reference： [1]Helvetica Chimica Acta,vol. 9,p. 429,430

34
[ 69-93-2 ]
[ 7732-18-5 ]
[ 127-08-2 ]
potassium persulfate [ No CAS ]
[ 97-59-6 ]

Reference： [1]Journal fur praktische Chemie (Leipzig 1954),vol. <2>106,p. 115,136,141

35
[ 69-93-2 ]
[ 7697-37-2 ]
[ 120-89-8 ]

Reference： [1]Justus Liebigs Annalen der Chemie,vol. 172,p. 74

36
[ 69-93-2 ]
[ 7732-18-5 ]
manganese dioxide [ No CAS ]
[ 120-89-8 ]

Reference： [1]Zeitschrift fur Chemie,1866,p. 746

37
[ 69-93-2 ]
potassium chlorate [ No CAS ]
[ 120-89-8 ]

Reference： [1]Justus Liebigs Annalen der Chemie,vol. 68,p. 24
Annales de Chimie (Cachan, France),vol. <3>24,p. 176

38
[ 69-93-2 ]
[ 7732-18-5 ]
potassium persulfate [ No CAS ]
[ 120-89-8 ]

Reference： [1]Journal fur praktische Chemie (Leipzig 1954),vol. <2>106,p. 115,136,141

39
[ 7647-01-0 ]
[ 69-93-2 ]
[ 7722-84-1 ]
[ 120-89-8 ]
[ 61066-33-9 ]

Reference： [1]Chemische Berichte,vol. 59,p. 721

40
[ 69-93-2 ]
[ 7664-93-9 ]
[ 7722-84-1 ]
[ 120-89-8 ]
[ 61066-33-9 ]

Reference： [1]Chemische Berichte,vol. 59,p. 721

41
[ 69-89-6 ]
dog's spleen-extract [ No CAS ]
[ 69-93-2 ]

Reference： [1]Journal of Biological Chemistry,1912,vol. 11,p. 32

42
[ 7732-18-5 ]
[ 69-89-6 ]
oxygen [ No CAS ]
bovine liverextract [ No CAS ]
[ 69-93-2 ]

Reference： [1]Hoppe-Seyler's Zeitschrift fur Physiologische Chemie,1904,vol. 43,p. 499

43
[ 807306-71-4 ]
[ 69-89-6 ]
xanthine oxide ase [ No CAS ]
[ 69-93-2 ]