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Chemical Structure| 701-44-0 Chemical Structure| 701-44-0

Structure of Nudifloramide
CAS No.: 701-44-0

Chemical Structure| 701-44-0

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Nudifloramide (2PY) is one of the final degradation products of nicotinamide adenine dinucleotide (NAD) and significantly inhibits PARP-1 activity in vitro.

Synonyms: 2PY; N-methyl-2-Pyridone-5-Carboxamide

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Product Citations

Product Citations

Dutta, Tumpa ; Kapoor, Nidhi ; Mathew, Meril ; Chakraborty, Suban S. ; Ward, Nathan P. ; Prieto-Farigua, Nicolas , et al.

Abstract: Metabolic routing of nicotinamide (NAM) to NAD+ or 1-methylnicotinamide (MeNAM) has impacts on human health and aging. NAM is imported by cells or liberated from NAD+. The fate of 2H4-NAM in cultured cells, mice, and humans was determined by stable isotope tracing. 2H4-NAM is an NAD+ precursor via the salvage pathway in cultured A549 cells and human PBMCs and in A549 cell xenografts and PBMCs from 2H4-NAM-dosed mice and humans, resp. 2H4-NAM is a MeNAM precursor in A549 cell cultures and xenografts, but not isolated PBMCs. NAM released from NAD+ is a poor MeNAM precursor. Addnl. A549 cell tracer studies yielded further mechanistic insight. NAMPT activators promote NAD+ synthesis and consumption. Surprisingly, NAM liberated from NAD+ in NAMPT activator-treated A549 cells is also routed toward MeNAM production Metabolic fate mapping of the dual NAM sources across the translational spectrum (cells, mice, humans) illuminates a key regulatory node governing NAD+ and MeNAM synthesis.

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Product Details of Nudifloramide

CAS No. :701-44-0
Formula : C7H8N2O2
M.W : 152.15
SMILES Code : O=C(C(C=C1)=CN(C)C1=O)N
Synonyms :
2PY; N-methyl-2-Pyridone-5-Carboxamide
MDL No. :MFCD00457283
InChI Key :JLQSXXWTCJPCBC-UHFFFAOYSA-N
Pubchem ID :69698

Safety of Nudifloramide

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Related Pathways of Nudifloramide

epigenetics
DNA
RTK

Isoform Comparison

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

6.57mL

1.31mL

0.66mL

32.86mL

6.57mL

3.29mL

65.72mL

13.14mL

6.57mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2
The prepared working fluid is recommended to be prepared now and used up as soon as possible in a short period of time. The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1

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