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Chemical Structure| 816-94-4 Chemical Structure| 816-94-4
Chemical Structure| 816-94-4

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1,2-DSPC is a phospholipid containing the saturated long-chain (18:0) stearic acid inserted at the sn-1 and sn-2 positions, which is commonly used in the generation of liposomes.

Synonyms: 1,2-Distearoyl-sn-glycero-3-PC; DSPC; Coatsome MC 8080

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Product Citations

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Kumar, Naveen ; Hazell, Nicholas C ; Bei, Jiani ; Nguyen, Tina ; Hu, Haitao ;

Abstract: In vitro transcription (IVT) is a widely used technique for mRNA synthesis in both basic research and the development mRNA-based vaccines and therapies. The efficiency of IVT critically depends on the quality and integrity of the linear DNA templates. The conventional method for template DNA preparation involves plasmid propagation in bacteria followed by enzymatic linearization, which is labor-intensive and costly. Here, we describe a cell-free, PCR-based approach for generating high-quality, high-yield linear DNA templates. We extensively compared the PCR-based method with the conventional plasmid-based approach in terms of IVT efficiency, mRNA production, and the immunogenicity of the resulting mRNA-LNP (lipid nanoparticle) vaccines. Compared to the plasmid-derived DNA, the PCR-based method yielded higher amounts of both DNA templates and transcribed mRNA, while maintaining mRNA quality and integrity. Importantly, mRNA-LNP vaccines encoding the SARS-CoV-2 spike protein, generated from both methods, elicited robust and comparable immune responses in mice, with no significant differences observed between the two template methods. Our findings highlight the advantages of PCR-generated DNA templates as a rapid, efficient, and cost-effective alternative for mRNA synthesis, with broad applications in vaccine and therapeutic development.

Keywords: DNA template ; mRNA synthesis ; In vitro Transcription (IVT) ; mRNA vaccines and therapies

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Xuexiang Han ; Junchao Xu ; Ying Xu ; Mohamad-Gabriel Alameh ; Lulu Xue ; Ningqiang Gong , et al.

Abstract: The ionizable lipidoid is a key component of nanoparticles (LNPs). Degradable lipidoids containing extended alkyl branches have received tremendous attention, yet their optimization and investigation are underappreciated. Here, we devise an in situ construction method for the combinatorial synthesis of degradable branched (DB) lipidoids. We find that appending branch tails to inefficacious lipidoids via degradable linkers boosts mRNA delivery efficiency up to three orders of magnitude. Combinatorial screening and systematic investigation of two libraries of DB-lipidoids reveal important structural criteria that govern their in vivo potency. The DB-LNP demonstrates robust delivery of mRNA therapeutics and gene editors into the liver. In a diet-induced obese mouse model, we show that repeated administration of DB-LNP encapsulating mRNA encoding human fibroblast growth factor 21 alleviates obesity and fatty liver. Together, we offer a construction strategy for high-throughput and cost-efficient synthesis of DB-lipidoids. This study provides insights into branched lipidoids for efficient mRNA delivery.

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Alternative Products

Product Details of 1,2-Distearoyl-sn-glycero-3-phosphorylcholine

CAS No. :816-94-4
Formula : C44H88NO8P
M.W : 790.15
SMILES Code : O=P(OC[C@H](OC(CCCCCCCCCCCCCCCCC)=O)COC(CCCCCCCCCCCCCCCCC)=O)([O-])OCC[N+](C)(C)C
Synonyms :
1,2-Distearoyl-sn-glycero-3-PC; DSPC; Coatsome MC 8080
MDL No. :MFCD00036905
InChI Key :NRJAVPSFFCBXDT-HUESYALOSA-N
Pubchem ID :94190

Safety of 1,2-Distearoyl-sn-glycero-3-phosphorylcholine

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H317-H319
Precautionary Statements:P280-P305+P351+P338
 

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