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With sulfuric acid; at 120 - 155℃;Industrial scale;
So the metering groove 237 kg aniline by adding the pot, to start stirrer 441 kg concentration of greater than 98% of concentrated sulfuric acid, in the case of the vicious circle, by adding 300 kg of 1-naphthylamine-8-sulfonic acid to continue heating, dehydrating atmosphere.When the affect reach 120-130 C time, dehydration-end, to the volume of the material in the measurement, if it is lower than the normal volume, the aniline is added.Continued heating to 155 ±2 C, then maintaining 10-12 hours, sampling analysis, 1-naphthylamine-8-sulfonic acid content ? 0.6% to be qualified, the qualified to the vacuum distillation pot, otherwise prolonged holding time.Follow-up steps of the embodiment with the specific embodiment 1 the same.The embodiment of the preparation obtained N-phenyl-1-naphthylamine-8-sulfonic acid, its yield is 94%, the purity is 98%.
8-anilinonaphthalene-1-sulfonic acid complex with chemokine CXCL3[ No CAS ]
Yield
Reaction Conditions
Operation in experiment
In aq. phosphate buffer; at 25℃;pH 6.0;
Fluorescence life time measurements were carried out using thetime correlated single photon counting spectrometer provided byHoriba Jobin Yvon (Fluorocube fluoresence life time system with NanoLED) for the samples ANS alone, ANS:CXCL2 and ANS:CXCL3 as describedelsewhere [25]. To monitor the life time analysis, CXCL2 andCXCL3 samples of 50 muM in 20mM Tris (pH 7), 50mM NaCl buffer anda fixed concentration of 1:5 (protein: ANS) were used to record thelifetime data.