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Chemical Structure| 24880-45-3 Chemical Structure| 24880-45-3
Chemical Structure| 24880-45-3

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Docosapentaenoic acid (22n-3, DPA) is an omega-3 polyunsaturated fatty acid that plays an important role in anti-inflammatory responses, cardiovascular health, and neuroprotection. DPA is involved in regulating various signaling pathways such as NF-κB and PPAR pathways, offering potential benefits in inflammation and metabolic regulation.

Synonyms: Docosapentaenoic acid(all cis-7,10,13,16,19); C22:5(all cis-7,10,13,16,19)Fatty acid

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Lee, Hyejin ; Yoon, Hye-Ran ;

Abstract: We developed and validated a fast, sensitive, and accurate anal. method using ultra high-pressure liquid chromatog.-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) for simultaneous extraction and quantification of 5 targeted omega fatty acids on human dried serum spots. MS/MS was performed with multiple reaction monitoring (MRM) mode under neg. electrospray ionization. We targeted omega fatty acids which are likely to have a pos. effect on various inflammatory reactions such as asthma, chronic obstructive pulmonary diseases and rheumatoid arthritis. If applicable, it is necessary to determine them as asthma biomarkers for intervention and public health. The coefficient of determination (r2) of calibration curve exhibited a good linear relationship between 0.9942 and 0.9963 in a range of 0.05-5μg mL-1. The anal. method was validated with excellent sensitivity (limit of detection; 0.005-0.01μg mL-1, limit of quantification; 0.03-0.1μg mL) that allows targeted analyses of omega fatty acids. We obtained the recoveries of 92.6 to 110.4% (RSD, 0.2-9.6%) for intraday and 92.6 to 107.6% (RSD, 1.4-7.0%) for interday. Clin. correlation between the healthy subjects and asthmatic patients sera was evaluated with the ratio of omega-3/omega-6 fatty acids. The developed method combines the advantages of easy handling dried filter paper and UHPLC-MS/MS for effectiveness of extraction and analyses step. We evaluated the validation for the quantification and applicability of human serum as a possible role of asthma biomarkers.

Keywords: Omega fatty acids ; Dried human serum spot ; UHPLC-MS ; MS ; Negative ionization ; Asthma

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Hyejin Lee ; Hye-Ran Yoon ;

Abstract: Omega fatty acids play an important role as biomarkers of chronic inflammatory diseases such as asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, and inflammatory bowel disease. The purpose of our study was to develop a new analytical method for the detection of omega fatty acids from dried serum spots. We developed an ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS)-based method in the multiple reaction monitoring (MRM) mode with negative ionization. The detected MRM transitions for five omega fatty acids are as follow: α-linolenic acid (m/z=277.20→277.20), eicosapentaenoic acid (m/z=301.10→257.30), docosahexaenoic acid (m/z=327.00→283.15), arachidonic acid (m/z=303.20→259.25), and docosapentaenoic acid (m/z= 329.10→285.25). The calibration curve showed an excellent linearity within 0.1-2.5 μg/mL range (R2=0.9947~0.9994). The analytical methods howed excellent sensitivity (LOD: 0.005-0.01 μg/mL, LOQ: 0.03-0.1 μg/mL), which would allow for the targeted analyses of omega fatty acids. The recoveries of the omega fatty acid from the dried serum spots were 92.6%-110.4% (RSD: ±0.2%-9.6%) and the retention time was within 5 min. This improved method offers rapid and sensitive quantification of omega fatty acids on dried serum spots using a small volume of serum and is expected to be applied to various biological specimens.

Keywords: Omega fatty acid ; Dried serum spot ; Negative ionization ; UPLC-MS/MS ; Asthma

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Product Details of Docosapentaenoic acid 22n-3

CAS No. :24880-45-3
Formula : C22H34O2
M.W : 330.50
SMILES Code : CC/C=C\C/C=C\C/C=C\C/C=C\C/C=C\CCCCCC(O)=O
Synonyms :
Docosapentaenoic acid(all cis-7,10,13,16,19); C22:5(all cis-7,10,13,16,19)Fatty acid
MDL No. :MFCD00156103
InChI Key :YUFFSWGQGVEMMI-JLNKQSITSA-N
Pubchem ID :5497182

Safety of Docosapentaenoic acid 22n-3

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P280-P301+P312-P302+P352-P305+P351+P338
 

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