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Chemical Structure| 38183-12-9 Chemical Structure| 38183-12-9

Structure of Fluorescamine
CAS No.: 38183-12-9

Chemical Structure| 38183-12-9

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Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products.

Synonyms: Ro 20-7234; Fluram

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Product Citations

Product Citations

Stiepel, Rebeca T. ; Batty, Cole J. ; MacRaild, Christopher A. ; Norton, Raymond S. ; Bachelder, Eric ; Ainslie, Kristy M.

Abstract: Malaria remains a global health threat, with significant morbidity and mortality worldwide despite current interventions. The human disease is caused by five different parasitic species, with Plasmodium falciparum being the deadliest. As a result, vaccine research against P. falciparum is a global priority. Merozoite surface protein 2 (MSP2) is a promising vaccine antigen as MSP2-specific antibodies have been shown previously to be protective against malaria infection. In this study, the formulation of an MSP2 vaccine was explored to enhance antigen uptake and achieve both an antibody and Th1 immune response by adsorbing MSP2 antigen onto a biomaterial carrier system. Specifically, MSP2 antigen was adsorbed onto acetalated dextran (Ace-DEX) microparticles (MPs). IgG and IgG2a titers elicited by the Ace-DEX MP platform were compared to titer levels elicited by MSP2 adsorbed to an FDA-approved alum adjuvant, MSP2 alone, and PBS alone. Both adsorption of MSP2 to Ace-DEX MPs and to alum elicited antibody responses in vivo, but only the formulation containing Ace-DEX MPs was able to elicit a significant Th1-biased response needed to combat the intracellular pathogen. As such, MSP2 adsorbed to Ace-DEX MPs demonstrates promise as a malaria vaccine.

Keywords: Antibody response ; Antigen adsorption ; Cross presentation ; Electrospray ; Polymeric particles ; Th1/Th2 response

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Product Details of Fluorescamine

CAS No. :38183-12-9
Formula : C17H10O4
M.W : 278.26
SMILES Code : O=C1C2(OC(C3=C2C=CC=C3)=O)OC=C1C4=CC=CC=C4
Synonyms :
Ro 20-7234; Fluram
MDL No. :MFCD01860207
InChI Key :ZFKJVJIDPQDDFY-UHFFFAOYSA-N
Pubchem ID :37927

Safety of Fluorescamine

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H315-H319-H335
Precautionary Statements:P280-P302+P352-P305+P351+P338-P261

Isoform Comparison

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.59mL

0.72mL

0.36mL

17.97mL

3.59mL

1.80mL

35.94mL

7.19mL

3.59mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2
 

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