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Chemical Structure| 115144-35-9 Chemical Structure| 115144-35-9

Structure of D-Luciferin potassium
CAS No.: 115144-35-9

Chemical Structure| 115144-35-9

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D-Luciferin is the natural substrate of luciferase (Luc), catalyzing the characteristic yellow-green light emission of fireflies. The luciferase (Luc) gene is commonly used as a reporter gene in research and active molecule screening, ideal for detecting low levels of gene expression due to minimal background interference from chemiluminescence.

Synonyms: Firefly luciferin potassium; Beetle Luciferin potassium; D-Luciferin (potassium salt)

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Product Citations

Product Citations

Lin, Chuman ; Li, Zhiyang ; Zhu, Xiaotong ; Zhou, Wanbing ; Lu, Xiansheng ; Zheng, Jiali , et al.

Abstract: The abnormal arginine metabolism is characteristic of tumor cell metabolism in colorectal cancer (CRC). However, the mechanisms underlying arginine metabolic reprogramming and how altered metabolism in turn enhances CRC tumorigenicity are poorly understood. Protein post-translational modifications (PTMs) are crucial for regulating protein function, activity, and interactions. Here, the study reports that arginine levels are elevated in CRC, accompanied by the high expression of arginase-1 (ARG1) but low levels of ARG1 β-hydroxybutyrylation (Kbhb) and its oncogenic role in CRC in a catalytic-activity-independent manner. Mechanistically, low-level ARG1-Kbhb-induced arginine metabolic reprogramming by decreasing the interaction of ARG1 with SLC3A2 in CRC cells inhibits the efflux of arginine, thereby increasing intracellular arginine levels to promote tumorigenicity. P300 is identified as the “writer” of Kbhb. Inducing ARG1-Kbhb at the Lys313 residue by β-hydroxybutyrate (BHB) promotes the interaction of ARG1 with SLC3A2, resulting in the efflux of arginine in CRC cells. Together, these findings reveal valuable insights into arginine metabolism reprogramming involving the ARG1-Kbhb/P300/SLC3A2 signaling axis, thereby bridging the connection between metabolic reprogramming and PTMs, which may shed light on the therapeutic potential of combining BHB with ARG1 inhibitor through the conventional enzymatic role and nonenzymatic metabolic function of ARG1 for CRC.

Keywords: β-hydroxybutyrylation ; ARG1 ; arginine metabolism reprogramming ; colorectal cancer

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Product Details of D-Luciferin potassium

CAS No. :115144-35-9
Formula : C11H7KN2O3S2
M.W : 318.41
SMILES Code : O=C([C@@H]1N=C(C2=NC3=CC=C(O)C=C3S2)SC1)[O-].[K+]
Synonyms :
Firefly luciferin potassium; Beetle Luciferin potassium; D-Luciferin (potassium salt)
MDL No. :MFCD00044928

Safety of D-Luciferin potassium

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Isoform Comparison

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.14mL

0.63mL

0.31mL

15.70mL

3.14mL

1.57mL

31.41mL

6.28mL

3.14mL

Dissolving Methods
The prepared working fluid is recommended to be prepared now and used up as soon as possible in a short period of time. The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1

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