CAS No. :	154447-36-6
Formula :	C₁₉H₁₇NO₃
M.W :	307.34
SMILES Code :	O=C1C=C(N2CCOCC2)OC3=C1C=CC=C3C4=CC=CC=C4
Synonyms :	SF 1101; NSC 697286
MDL No. :	MFCD00270881
InChI Key :	CZQHHVNHHHRRDU-UHFFFAOYSA-N
Pubchem ID :	3973

GHS Pictogram:
Signal Word:	Warning
Hazard Statements:	H302-H315-H319-H332-H335
Precautionary Statements:	P261-P280-P305+P351+P338

BMSCs	20 µM	0, 15, 30, 60, 120 min	LY294002 significantly inhibited the higher expression of osteogenic genes induced by Ginsenoside Rb1.	Int J Oral Sci. 2022 Feb 14;14(1):10.
Astrocytes	10 µM	1 hour	AMPK inhibition reduces collective cell migration and invasion efficiency.	Nat Commun. 2022 Aug 11;13(1):4528.
TNBC cell lines	2 µM	10 days	To investigate the effect of MAPK4 knockdown on cell growth and sensitivity to PI3K inhibitors	Nat Commun. 2022 Jan 11;13(1):245.
HWJ-MSCs	40 µM	2 hours	Estimate the effects of the AKT inhibitor on the proliferation of FP2-cultured hWJ-MSCs.	J Adv Res. 2024 Aug;62:119-141.
SKMEL28 melanoma cells	20 µM	2 hours	The study involved the inhibition of AKT signaling to determine the effects on oxidative DNA damage repair; results showed reduced expression of OGG.	Redox Biol. 2024 Jun;72:103135.
U-251 and Ca9-22 cells	20 µM	2 hours	To assess the impact of PI-3 kinase inhibition on cell behavior.	Adv Sci (Weinh). 2023 Mar;10(7):e2207368.
ADSCs	50 µM	24 hours	To investigate the role of PI3K signaling on rGO/PCL induced neurotrophic phenotype changes and myelin protein secretion.	Bioact Mater. 2022 Jun 13;20:319-338.
MLE-12 cells	50 μg/mL	24 hours	LY294002 was used as a positive control to inhibit up-regulated expression of fibrosis-related genes.	J Nanobiotechnology. 2024 Oct 26;22(1):662.
RAW 264.7	10 µM	24 hours	To evaluate the effect of LY294002 on macrophage polarization.	J Nanobiotechnology. 2024 Jun 8;22(1):322.
HESCs	10 µM	24 hours	To investigate the role of LY294002 in influencing differentiation to endoderm.	Nat Commun. 2023 Jan 25;14(1):405.
Primary chondrocytes	10 µM	24 hours	LY294002 administration significantly reduced SNP-induced apoptosis activities and alleviated aberrant force-induced chondrocyte degeneration.	Int J Oral Sci. 2022 Jul 14;14(1):34.
NHA/HRasV12/TRIM24 cells	0.01 to 100 µM	24, 48, and 72 hours	Cell proliferation was evaluated and the half-maximal inhibitory concentration (IC50) values were determined from fitted concentration-response curves.	Adv Sci (Weinh). 2024 Aug;11(29):e2400023.
Chondrocytes	20 µM	3 days	The study aims to investigate the effects of blood on chondrocyte viability and apoptosis.	Signal Transduct Target Ther. 2024 May 8;9(1):109.
H9C2 cells	25 µM	30 min	To explore the mechanisms of NGR1's protective effect on H9C2 cells.	Redox Biol. 2022 Aug;54:102384.
HAEC and HSMEC	50 nM	30 min	To evaluate the effect of LY294002 on insulin uptake.	Nat Commun. 2023 Aug 17;14(1):4989.
Vascular Smooth Muscle Cells (VSMC)	50 µM	30 min	To inhibit the activation of Akt and study the effects on related signaling pathways.	Redox Biol. 2022 Jun;52:102296.
Human fetal RPE cells	2 µM	4 days	To investigate the effect of glucose concentration on inflammatory responses, where inflammatory markers were evaluated.	Nat Commun. 2022 Oct 13;13(1):6045.
CTLL-2 cells	50 µM	48 hours	The purpose was to analyze the effect of LY294002 on the CTLL-2 cells in the presence of IL-7.	Bioact Mater. 2023 Sep 26;32:12-27.
RF24 cells	50 µM	6 hours	The treatment resulted in reduced angiogenesis as inferred from subsequent experiments.	Nat Commun. 2023 Apr 26;14(1):2407.
HUVECs	5 μg/mL	6, 24, 48, and 72 hours	To observe the effects of the PI3K inhibitor as a negative control	Biomaterials. 2022 Sep;288:121729.
Human embryonic stem cells	5 µM	8 days	Induction of neuroectoderm differentiation.	Nucleic Acids Res. 2024 May 22;52(9):4935-4949.
Astrocytes	40 µM		To inhibit PI3K signaling pathway and evaluate its effects on glycophagy.	Redox Biol. 2024 Aug;74:103234.
RAW264.7 cells	25 µM	1 h	Inhibit PI3K signaling pathway and reduce M1 macrophage polarization	J Transl Med. 2024 Aug 2;22(1):717.
BMDMs	25 µM	1 h	Inhibit PI3K signaling pathway and reduce M1 macrophage polarization	J Transl Med. 2024 Aug 2;22(1):717.
neonatal rat cardiomyocytes (NRCMs)	10 μM	2 h	Inhibited PI3K α/AKT signaling, reversed FGF7-mediated promotion of Nrf2 nuclear translocation, and increased ROS levels and apoptotic cells	Redox Biol. 2022 Oct;56:102468.
human dental pulp stem cells	10 µM	7 days	LY294002 attenuated the responsiveness of 10−6 M insulin to IIS/PI3K/AKT/mTOR pathway axis, suppressing the promoting effect of insulin on cell proliferation, osteogenic differentiation and bone formation.	Stem Cell Res Ther. 2024 Jul 29;15(1):227.
PC-9R cells	25 μM		Inhibited the PI3K/AKT pathway and restored sensitivity to gefitinib in PC-9R cells	J Cancer. 2022 Mar 21;13(6):1837-1847.
BMDMs	0.5 μM	2 h	Inhibited PI3K/Akt/Nrf2 signaling pathway and increased NLRP3/Caspase-1/GSDMD protein expression	Phytomedicine. 2023 Jun;114:154753.
A549 cells	10μM	1 h	Inhibited PI3K/AKT signaling pathway, alleviated TGF-β1-induced epithelial-mesenchymal transition (EMT)	Front Pharmacol. 2022 Mar 9;13:829673.
Huh7-SR cells	5 µM	1 h	Inhibited PI3K/Akt pathway, reduced Nrf2 nuclear translocation, and increased ferroptosis level	Front Oncol. 2022 Mar 22;12:852095.
HepG2-SR cells	5 µM	1 h	Inhibited PI3K/Akt pathway, reduced Nrf2 nuclear translocation, and increased ferroptosis level	Front Oncol. 2022 Mar 22;12:852095.
Mouse primary hepatocytes	10 μM	1.5 h	To evaluate the relationship between PI3K/AKT pathway and ferroptosis, LY294002 pretreatment exacerbated ferroptosis	Transplantation. 2024 Nov 1;108(11):e357-e369.

Mice	Experimental autoimmune hepatitis model	Intraperitoneal injection	30 mg/kg	Pretreatment 30 minutes before	Inhibit PI3K signaling pathway and reduce FGF4's regulatory effect on M1 macrophages	J Transl Med. 2024 Aug 2;22(1):717.
C57BL/6 mice	Myocardial infarction model (LAD ligation)	Intraperitoneal injection	2.5 mg/kg	From LAD operation to 7 days post-operation	Inhibited PI3K α/AKT signaling, abolished FGF7-mediated regulation of Nrf2 and HXK2, and exacerbated myocardial oxidative stress and apoptosis	Redox Biol. 2022 Oct;56:102468.
BALB/c nude mice	PC-9R cell xenograft model	Intraperitoneal injection	2.5 mg/kg	Every three days for 12 days	LY294002 significantly reduced tumor volume and enhanced the anti-tumor effect of gefitinib	J Cancer. 2022 Mar 21;13(6):1837-1847.
C57BL/6 mice	Acute lung injury model	Injection	20 mg/kg	24 hours	Inhibited PI3K/Akt/Nrf2 signaling pathway and increased NLRP3/Caspase-1/GSDMD protein expression	Phytomedicine. 2023 Jun;114:154753.
Mice	Bleomycin-induced pulmonary fibrosis model	Intraperitoneal injection	25 mg/kg	Every other day for 21 days	Inhibited PI3K/AKT signaling pathway, alleviated bleomycin-induced pulmonary fibrosis and EMT	Front Pharmacol. 2022 Mar 9;13:829673.
NOD mice	Diabetes model	Intraperitoneal injection	75 mg/kg	Daily for 3 days	Inhibition of PI3K signaling pathway to observe its effect on CD4+ T cell migration	J Immunol. 2016 Nov 1;197(9):3504-3511
NOD-SCID mice	Subcutaneous tumor model	Intraperitoneal injection	25 mg/kg	Twice a week for 4 weeks	Inhibited PI3K/Akt pathway, slowed growth of FNDC5-overexpressing HCC cells, and increased ferroptosis level	Front Oncol. 2022 Mar 22;12:852095.
C57 mice	Liver warm ischemia/reperfusion model	Intraperitoneal injection	10 mg/kg	Single dose 36 h before liver ischemia followed by 6 h reperfusion	To assess the role of PI3K/AKT pathway in hepatic I/R injury, LY294002 pretreatment abolished the protective effect of rmGas6 and exacerbated ferroptosis	Transplantation. 2024 Nov 1;108(11):e357-e369.

Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
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Code	Phrase
P201	Obtain special instructions before use.
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P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
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P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
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P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
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P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
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P320
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P321
P322
P330	Rinse mouth.
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P337	If eye irritation persists:
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P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
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P378
P380	Evacuate area.
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P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
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P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
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P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
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P422
P402 + P404	Store in a dry place. Store in a closed container.
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P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling

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CAS No.: 154447-36-6

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CAS No.: 154447-36-6

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Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
H224	Extremely flammable liquid and vapour
H225	Highly flammable liquid and vapour
H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed