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Chemical Structure| 76931-93-6 Chemical Structure| 76931-93-6
Chemical Structure| 76931-93-6

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SATA Protein Modifier is a protein modification agent used to protect free amine residues in proteins and peptides.

Synonyms: SATA

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Product Citations

Product Citations

Shah, Shrey ; Ranasinghe, Meenakshi ; Decker, John ; Fraser, Keith ; Friedman, Adam ; Wang, Ying , et al.

Abstract: In vivo genetic engineering of T cells could overcome the logistical, biological, and safety challenges of ex vivo modification, but effective and safe delivery systems remain limited by a lack of cellular specificity. Here, we developed aptamer-functionalized lipid nanoparticles (LNPs) for targeted mRNA delivery to CD4+ T cells, employing both a validated CD4-binding aptamer (Apt62) and novel aptamers generated using our proprietary transformer-based AI language model, AptaBLE. LNPs formulated with ionizable lipid SM102 or MC3 and conjugated with aptamers at controlled densities were physiochemically characterized and assessed for binding, in vitro transfection, in vivo biodistribution, and safety evaluation. Aptamer-functionalized LNPs demonstrated selective nanomolar binding to recombinant CD4, achieved enhanced transfection of CD4+ versus CD4- T cells in vitro, and significantly enriched mRNA delivery to immune-rich tissues in vivo, achieving up to 70-fold spleen signal enhancement with SM102 formulations compared to non-targeted controls, while maintaining suitable safety profiles. Overall, these findings demonstrate aptamer-functionalized LNPs, augmented by AI-guided aptamer design, as a tunable, non-immunogenic platform for in vivo T cell engineering.

Keywords: Aptamers ; AI-guided design ; Immune targeting ; Active targeting

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Product Details of N-Succinimidyl-S-acetylthioacetate

CAS No. :76931-93-6
Formula : C8H9NO5S
M.W : 231.23
SMILES Code : O=C(ON1C(CCC1=O)=O)CSC(C)=O
Synonyms :
SATA
MDL No. :MFCD00036891
InChI Key :FLCQLSRLQIPNLM-UHFFFAOYSA-N
Pubchem ID :127532

Safety of N-Succinimidyl-S-acetylthioacetate

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338
 

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