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There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.

Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Chemical Structure| 298-83-9 Chemical Structure| 298-83-9
Chemical Structure| 298-83-9

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NBT is a substrate for dehydrogenases and is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures.

Synonyms: NBT; Nitro Blue Tetrazolium (chloride); NSC 27622

4.5 *For Research Use Only !

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Product Citations

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Podbielska, Magdalena ; Kus-Liśkiewicz, Małgorzata ; Płoch, Dariusz ; Szpyrka, Ewa ;

Abstract: Polyethylene microplastics (PE-MPs) and glyphosate (GLY) are widespread aquatic contaminants, but their combined effects on microalgae remain poorly understood. This study assessed the individual and joint toxicity of GLY and PE-MPs to the model microalga Chlorella vulgaris. Acute (3-day) and chronic (7-day) exposures were performed using GLY at 1–40 mg/L, alone or combined with PE-MPs (10 mg/L). A four-parameter log-logistic (4PL) model was applied to estimate median effect concentrations (EC50). After 72 h, the EC50 values were 9.77 mg/L for the GLY single system and 2.31 mg/L for the GLY-PE combined system, confirming enhanced toxicity in combined exposures. Co-exposure reduced pigment levels (chlorophyll a, chlorophyll b, and carotenoids) by up to 65% and significantly increased oxidative stress markers, including reactive oxygen species production and malondialdehyde accumulation, compared with single treatments. Antioxidant enzymes (superoxide dismutase and catalase) showed concentration- and time-dependent responses, indicating activation of cellular defense mechanisms. Scanning Electron Microscopy revealed PE-induced aggregation and structural damage to algal cells, particularly at higher GLY concentrations. These findings demonstrate that PE-MPs can amplify the toxic effects of GLY on microalgae and highlight the need for further studies at environmentally relevant concentrations and with different polymer types.

Keywords: polyethylene microplastics ; glyphosate ; Chlorella vulgaris ; combined toxicity ; oxidative stress

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Podbielska, Magdalena ; Kus-Liśkiewicz, Małgorzata ; Płoch, Dariusz ; Szpyrka, Ewa ;

Abstract: Polyethylene (PE) microplastics and glyphosate (GLY) are emerging ubiquitous contaminants in various aquatic environments. Little is known about their toxicity and combined influence on the alga Chlorella vulgaris. Our study provides reliable data supporting understanding of toxic effects to microalgae C. vulgaris of GLY in a single system and GLY-PE in a combined system after 3 and 7 days of exposure. Microalgae co-exposure to GLY and PE resulted in their greater growth inhibition, which is GLY concentration-dependent. The presence of PE increases toxicity and a synergistic effect was observed. The GLY-PE combined system resulted in a greater reduction in the pigment production. The antioxidant system was activated, indicating that pesticides and microplastics have a negative influence on microalgae. For the GLY-PE combined system, the levels of malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD), and catalase (CAT) were higher than those in the single system. Our results confirm that microplastics can exhibit a synergistic effect with other xenobiotics, like GLY and contribute to a significant increase in aquatic organisms exposure. As microplastics are ubiquitous in environment, more studies are needed to examine combine effects of microplastics - emerging contaminants to living organisms.

Keywords: Microplastics ; Polyethylene ; Algae ; Chlorella vulgaris ; Toxicity

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Alternative Products

Product Details of Nitro blue tetrazolium chloride

CAS No. :298-83-9
Formula : C40H30Cl2N10O6
M.W : 817.64
SMILES Code : COC1=C(C=CC(C2=CC(OC)=C([N+]3=NC(C4=CC=CC=C4)=NN3C5=CC=C([N+]([O-])=O)C=C5)C=C2)=C1)[N+]6=NC(C7=CC=CC=C7)=NN6C8=CC=C([N+]([O-])=O)C=C8.[Cl-].[Cl-]
Synonyms :
NBT; Nitro Blue Tetrazolium (chloride); NSC 27622
MDL No. :MFCD00012159
InChI Key :FSVCQIDHPKZJSO-UHFFFAOYSA-L
Pubchem ID :9281

Safety of Nitro blue tetrazolium chloride

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H318
Precautionary Statements:P280-P305+P351+P338
Class:8
UN#:1759
Packing Group:
 

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