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Chemical Structure| 298-93-1 Chemical Structure| 298-93-1
Chemical Structure| 298-93-1

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MTT (thiazolyl blue tetrazolium bromide), a yellow dye, is used in measurement of cell proliferation. Succinate dehydrogenase in living cell mitochondria can reduce exogenous MTT to water-insoluble blue-purple crystalline formamidine.

Synonyms: MTT; Thiazolyl Blue Tetrazolium bromide; NSC 60102

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Marta Stolarek ; Kamil Kaminski ; Marta Kaczor-Kamińska ; Magdalena Obłoza ; Piotr Bonarek ; Anna Czaja , et al.

Abstract: A photoactive analogue of was synthesized with two arylazopyrazole ligands, able to undergo trans−cis/cis−trans photoisomerizations. The cis photoisomer showed a dark half-life of 9 days. The cytotoxicities of both photoisomers of the complex were determined in several cancer and normal cell lines and compared to that of . The trans photoisomer of the complex was much more cytotoxic than both the cis photoisomer and , and was more toxic for cancer (4T1) than for normal (NMuMG) murine breast cells. 4T1 cell death occurred through necrosis. Photoisomerization of the trans and cis photoisomers internalized by the 4T1 cells increased and decreased their viability, respectively. The cellular uptake of the trans photoisomer was stronger than that of both the cis photoisomer and . Both photoisomers interacted with DNA faster than . The trans photoisomer was bound stronger by bovine serum albumin and induced a greater decrease in cellular levels than the cis photoisomer.

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Ameerah A. Radhi ; Wedad K. Ali ; Fitua Al-Saedi ;

Abstract: Tamoxifen Citrate (TC) is the standard endocrine therapy for estrogen receptor (ER) positive breast cancer. TC is a selective estrogen receptor modulator (SERM) whose estrogenic properties in uterus have been linked to increased side effects like blood clots, endometrial polyps and cancer. Therefore, significant amount of research has been carried out to develop tamoxifen loaded nano-formulations with a preferential accumulation in tumor tissue rather than healthy tissues. Synthetic high-density lipoproteins (sHDL) are novel nanocarriers with inherent active-targeting ability towards tumor cells through the ligand–receptor interaction between apolipoprotein A-I (Apo A-I) and scavenger receptor class B type I (SR-BI) overexpressed in various malignant cells. The current study was carried out to investigate whether encapsulation of TC in sHDL could improve the cytotoxic effect of TC against malignant cells. For this purpose, the cytotoxicity of TC-sHDL was evaluated in MCF-7 cell line in vitro. MTT assay demonstrated the increased cytotoxicity of TC-sHDL against cancer cells as compared with the cytotoxic effect of the free drug.

Keywords: Tamoxifen Citrate ; breast cancer ; in vitro cytotoxicity

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Product Details of Thiazolyl Blue

CAS No. :298-93-1
Formula : C18H16BrN5S
M.W : 414.32
SMILES Code : CC1=C(C)N=C(N2[N+](C3=CC=CC=C3)=NC(C4=CC=CC=C4)=N2)S1.[Br-]
Synonyms :
MTT; Thiazolyl Blue Tetrazolium bromide; NSC 60102
MDL No. :MFCD00011964
InChI Key :AZKSAVLVSZKNRD-UHFFFAOYSA-M
Pubchem ID :64965

Safety of Thiazolyl Blue

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H315-H319-H335-H341
Precautionary Statements:P201-P202-P261-P302+P352-P305+P351+P338-P308+P313
 

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