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Chemical Structure| 298-93-1 Chemical Structure| 298-93-1
Chemical Structure| 298-93-1

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MTT (thiazolyl blue tetrazolium bromide), a yellow dye, is used in measurement of cell proliferation. Succinate dehydrogenase in living cell mitochondria can reduce exogenous MTT to water-insoluble blue-purple crystalline formamidine.

Synonyms: MTT; Thiazolyl Blue Tetrazolium bromide; NSC 60102

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Marta Stolarek ; Kamil Kaminski ; Marta Kaczor-Kamińska ; Magdalena Obłoza ; Piotr Bonarek ; Anna Czaja , et al.

Abstract: A photoactive analogue of was synthesized with two arylazopyrazole ligands, able to undergo trans−cis/cis−trans photoisomerizations. The cis photoisomer showed a dark half-life of 9 days. The cytotoxicities of both photoisomers of the complex were determined in several cancer and normal cell lines and compared to that of . The trans photoisomer of the complex was much more cytotoxic than both the cis photoisomer and , and was more toxic for cancer (4T1) than for normal (NMuMG) murine breast cells. 4T1 cell death occurred through necrosis. Photoisomerization of the trans and cis photoisomers internalized by the 4T1 cells increased and decreased their viability, respectively. The cellular uptake of the trans photoisomer was stronger than that of both the cis photoisomer and . Both photoisomers interacted with DNA faster than . The trans photoisomer was bound stronger by bovine serum albumin and induced a greater decrease in cellular levels than the cis photoisomer.

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Wang, Hongzheng ; Huang, Min ; Zhu, Mengyuan ; Su, Chi ; Zhang, Yijian ; Chen, Hongyu , et al.

Abstract: Background: Immune checkpoint inhibitors, which have attracted much attention in recent years, have achieved good efficacy, but their use is limited by the high incidence of acquired drug resistance. Therefore, there is an urgent need to develop new immunotherapy drugs. Compound taxus chinensis capsule (CTC) is an oral paclitaxel compound drug, clinical results showed it can change the number of regulatory T cells and T helper cell 17 in peripheral blood. Regulating the balance between regulatory T cells and T helper cell 17 is considered to be an effective anticancer strategy. Paclitaxel and ginsenoside metabolite compound K are the main immunomodulatory components, it is not clear that paclitaxel combined with compound K can inhibit tumor development by regulating the balance between regulatory T cell and T helper cell 17. Methods: MTT, EdU proliferation and plate colony formation assay were used to determine the concentration of paclitaxel and compound K. AnnexinV-FITC/PI staining, ELISA, Western Blot assay, Flow Cytometry and Immunofluorescence were used to investigate the effect of paclitaxel combined with compound K on Lewis cell cultured alone or co-cultured with splenic lymphocyte. Finally, transplanted tumor C57BL/6 mice model was constructed to investigate the anti-cancer effect in vivo. Results: According to the results of MTT, EdU proliferation and plate colony formation assay, paclitaxel (10 nM) and compound K (60 μM) was used to explore the mechanism. The results of Flow Cytometry demonstrated that paclitaxel combined with compound K increased the number of T helper cell 17 and decreased the number of regulatory T cells, which induced pyroptosis of cancer cells. The balance was mediated by the JAK–STAT pathway according to the results of Western Blot and Immunofluorescence. Finally, the in vivo results showed that paclitaxel combined with compound K significantly inhibit the progression of lung cancer. Conclusions: In this study, we found that paclitaxel combined with compound K can activate CD8+ T cells and induce pyroptosis of tumor cells by regulating the balance between regulatory T cells and T helper cell 17. These results demonstrated that this is a feasible treatment strategy for lung cancer.

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Ameerah A. Radhi ; Wedad K. Ali ; Fitua Al-Saedi ;

Abstract: Tamoxifen Citrate (TC) is the standard endocrine therapy for estrogen receptor (ER) positive breast cancer. TC is a selective estrogen receptor modulator (SERM) whose estrogenic properties in uterus have been linked to increased side effects like blood clots, endometrial polyps and cancer. Therefore, significant amount of research has been carried out to develop tamoxifen loaded nano-formulations with a preferential accumulation in tumor tissue rather than healthy tissues. Synthetic high-density lipoproteins (sHDL) are novel nanocarriers with inherent active-targeting ability towards tumor cells through the ligand–receptor interaction between apolipoprotein A-I (Apo A-I) and scavenger receptor class B type I (SR-BI) overexpressed in various malignant cells. The current study was carried out to investigate whether encapsulation of TC in sHDL could improve the cytotoxic effect of TC against malignant cells. For this purpose, the cytotoxicity of TC-sHDL was evaluated in MCF-7 cell line in vitro. MTT assay demonstrated the increased cytotoxicity of TC-sHDL against cancer cells as compared with the cytotoxic effect of the free drug.

Keywords: Tamoxifen Citrate ; breast cancer ; in vitro cytotoxicity

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Product Details of Thiazolyl Blue

CAS No. :298-93-1
Formula : C18H16BrN5S
M.W : 414.32
SMILES Code : CC1=C(C)N=C(N2[N+](C3=CC=CC=C3)=NC(C4=CC=CC=C4)=N2)S1.[Br-]
Synonyms :
MTT; Thiazolyl Blue Tetrazolium bromide; NSC 60102
MDL No. :MFCD00011964
InChI Key :AZKSAVLVSZKNRD-UHFFFAOYSA-M
Pubchem ID :64965

Safety of Thiazolyl Blue

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H315-H319-H335-H341
Precautionary Statements:P201-P202-P261-P302+P352-P305+P351+P338-P308+P313
 

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