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Chemical Structure| 54827-17-7 Chemical Structure| 54827-17-7
Chemical Structure| 54827-17-7

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TMB, a chromogenic substrate, is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

Synonyms: BM blue; Sure Blue TMB; 3,3′,5,5′-Tetramethylbenzidine

4.5 *For Research Use Only! Not for Human Use. We Do Not Sell to Patients.

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Product Citations

Product Citations

Karataş, Büşra ; Kara, Hayriye Eda Şatana ;

Abstract: Arsenic is a highly toxic metal with harmful properties for the environment, humans, and other living things. Nanomaterials that exhibit enzyme-like catalytic activity are called nanozymes. In this study, BSA-stabilized Pt nanozymes were synthesized and characterized using TEM, XPS, and Uv-vis spectroscopic methods. A peroxidase mimetic nanozymes were used for the determination of arsenic in water, urine, and serum samples based on nanoparticle-catalyzed oxidation of colorless (TMB) to blue oxTMB in the presence of H2O2. Arsenic selectively inhibited this oxidation process, causing a decrease in UV–Vis absorbance at 652 nm and solution discoloration. The average diameter of Pt nanoparticles was measured as 1–2 nm and possesses highly peroxidase-like activity with the Km values of 0.17 mM and 104 mM towards and H2O2, respectively. Under optimized conditions, arsenic was detected with a detection limit of 0.75 nM with a linear response range of 10–100 nM, which means the developed system is sensitive enough to detect As in drinking water, which has the maximum allowable limits of 10 μg/L (133 nM) defined by the World Health Organization (WHO) and the EPA. Recovery values were obtained 99 %–100.4 %–105.6 % for water, urine, and serum samples, respectively. AGREE calculator assessment (0.72), BAGI (72.5), and RAPI (90.0) confirmed the method's greenness, practical applicability, and offering advantages of minimal organic solvent consumption compared to existing techniques.

Keywords: Nanozyme ; Nanoparticle ; Platinum ; Arsenic ; Colorimetric detection

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Product Details of TMB

CAS No. :54827-17-7
Formula : C16H20N2
M.W : 240.34
SMILES Code : NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C
Synonyms :
BM blue; Sure Blue TMB; 3,3′,5,5′-Tetramethylbenzidine
English Name :3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine
MDL No. :MFCD00007748
InChI Key :UAIUNKRWKOVEES-UHFFFAOYSA-N
Pubchem ID :41206

Safety of TMB

Application In Synthesis of TMB

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Downstream synthetic route of [ 54827-17-7 ]

[ 54827-17-7 ] Synthesis Path-Downstream   1~19

  • 1
  • [ 54827-17-7 ]
  • [ 102368-13-8 ]
  • [ 322424-42-0 ]
YieldReaction ConditionsOperation in experiment
88% In dichloromethane at 20℃;
  • 2
  • [ 54827-17-7 ]
  • [ 376368-24-0 ]
  • [ CAS Unavailable ]
YieldReaction ConditionsOperation in experiment
92% With lithium chloride In 1-methyl-pyrrolidin-2-one; N,N,N,N,N,N-hexamethylphosphoric triamide at 20℃; for 24h;
  • 3
  • [ 24463-19-2 ]
  • [ 54827-17-7 ]
  • [ 448208-87-5 ]
YieldReaction ConditionsOperation in experiment
55% With triethylamine In toluene Heating;
  • 4
  • [ 54827-17-7 ]
  • [ 870284-60-9 ]
YieldReaction ConditionsOperation in experiment
82% With water-d2 at 180℃; for 24h; 11 Reference Example 11 Synthesis of Deuterated 3,3',5,5'-tetramethylbenzidine 3,3',5,5'-Tetramethylbenzidine of 10 g and 5% Pt/C of 2 g were added to deuterated water (D2O) of 340 mL and subjected to reaction at 180° C. for 24 hours. After termination of the reaction, the reaction product was purified similarly as in Reference Example 1 to obtain deuterated 3,3',5,5'-tetramethylbenzidine of 6.5 g (yield: 82%). The obtained deuterated 3,3',5,5'-tetramethylbenzidine was subjected to structural analysis by measuring its 1H-NMR and 2H-NMR spectra to show an average deuteration ratio of 65%.
54% With hydrogen; water-d2 In water-d2 at 180℃; for 24h;
54% With water-d2 at 180℃; for 24h; 3 Reference Example 3 Synthesis of Deuterated 3,3',5,5'-tetramethylbenzidine 3,3',5,5'-Tetramethylbenzidine of 10 g and a mixed catalyst of 3 g composed of 10% Pd/C of 1 g and 5% Pt/C of 2 g were added to deuterated water (D2O) of 340 mL and subjected to reaction at 180° C. for 24 hours. After termination of the reaction, the reaction solution was extracted with ethyl acetate, followed by filtering off the mixed catalyst. The obtained filtrate was dried using magnesium sulfate, concentrated under reduced pressure and then purified by column chromatography to obtain deuterated 3,3',5,5'-tetramethylbenzidine of 5.4 g (yield: 54%). The obtained deuterated 3,3',5,5'-tetramethylbenzidine was subjected to structural analysis by measuring its 1H-NMR and 2H-NMR spectra to show an average deuteration ratio of 98%
With hydrogen; water-d2 at 180℃; for 24h;

  • 5
  • [ 54827-17-7 ]
  • [ CAS Unavailable ]
YieldReaction ConditionsOperation in experiment
With dihydrogen peroxide; sodium dodecyl-sulfate; peroxidase In phosphate buffer
With dihydrogen peroxide; Microperoxidase-11 at 20℃; Enzymatic reaction;
With peroxidase In water Enzymatic reaction;
With copper hydroxide; dihydrogen peroxide In aq. acetate buffer at 25℃;
With zinc ferrite; dihydrogen peroxide In methanol
With dihydrogen peroxide In ethanol at 20℃;
With copper hydroxide; dihydrogen peroxide In aq. acetate buffer at 25℃;
In aq. acetate buffer at 30℃; for 0.166667h;
With oxygen In aq. buffer at 55℃; for 0.0833333h;
With dihydrogen peroxide; horseradish peroxidase In aq. phosphate buffer at 24.8 - 25.2℃; Enzymatic reaction; 4.4. Bioassay General procedure: The following experiments were measured under the sameconditions of 250.2 C and pH = 7.4. UV-vis spectra wereperformed by monitoring the absorbance at a specific time whenthe reaction occurs 5 min. Time-dependent curves were carried outin time course mode by monitoring the absorbance change at652 nm. The concentration of HRP, TMB and H2O2 is 0.05 mg/mL,200 mmol/L and 2 mmol/L respectively for all catalytic experimentunless otherwise stated. Firstly, 0.05 mg/mL HRP solutionsincubated for 1 h in different reaction mediums (pH = 7.4,250.2 C), then added 8 mL TMB (0.2 mol/L) stock solutionprepared in DMSO-d6 and 2 mL H2O2 (8.0 mmol/L) into theincubation mediums to obtain 8 mL reaction solutions, took3 mL sample for absorbance measurements.The kinetic assays of HRP-TMB-H2O2 were further conducted inthe mediums containing SDS (2.5 mmol/L) and SDS/[Emim][BF4](2.5 mmol/L/2.5 mmol/L) combination, kinetic parameters wereobtained by the enzyme kinetics theory and methods. The typicalMichaelis-Menten curve was obtained byfitting the Mentenequation. Kinetic assays were carried onfixing HRP concentrationat 0.05 mg/mL and varying concentrations of H2O2 and TMB.The activity assay of HRP was realized as follows. Firstly, 24 mLTMB stock solution (0.2 mol/L), 2 mL H2O2 (8.0 mol/L) were addedinto the mediums containing SDS/[Emim][BF4] (2.5 mmol/L/2.5 mmol/L) combinations, then added HRP solutions into the mixtures to obtain reaction solutions (8 mL) with different massconcentrations of HRP (10-50 mg/mL)and mixed quickly. Finally,took 3 mL sample for recording the absorbance at 652 nm within1 min.H2O2 detection was measured by the following steps. Firstly,8 mL TMB stock solution (0.2 mol/L), different volumes of H2O2were added into the mediums containing SDS/[Emim][BF4](2.5 mmol/L/2.5 mmol/L) combinations to obtain mixtures withdifferent H2O2 concentration (1 mmol/L-100 mmol/L). Then added4 mL HRP solution (0.1 mg/mL) into the mixtures to obtain reactionsolutions (8 mL) and mixed quickly. Finally, took 3 mL sample forrecording the absorbance at 652 nm at the specific time when thereaction occurred 3 min.
With C71H87N35O38P6*0.66C34H32ClFeN4O4; dihydrogen peroxide; ammonium chloride In dimethyl sulfoxide at 55℃;
With dihydrogen peroxide In aq. buffer at 20℃; for 0.25h;
With dihydrogen peroxide In water
With dihydrogen peroxide In aq. acetate buffer at 25℃; for 0.5h;
With gold; mercury ion
With manganese(IV) oxide
With cerium(IV) oxide; dihydrogen peroxide; adenosine triphosphate disodium In aq. phosphate buffer at 20℃;
With cerium(IV) sulphate In aq. acetate buffer at 25℃;
With oxygen In aq. acetate buffer at 25℃; UV-irradiation;
With dihydrogen peroxide In aq. acetate buffer
With copper cobaltite; dihydrogen peroxide In aq. phosphate buffer at 40℃;
With ultrasound triggered polyethylene glycol coated stanene based nanosheets In aq. acetate buffer at 21℃;
With cerium(IV) oxide; dihydrogen peroxide
With nickel-silver-graphene quantum dot-graphene hybrid In aq. acetate buffer at 37℃; for 0.283333h;
With carbon dot-coated magnetite supported on porphyrin-conjugated confeito-like gold nanoparticles In aq. acetate buffer Irradiation; Evaluation of Photodynamic Ability The oxygen photosensitizationperformance was determined from the quantificationof ROS using TMB as a probe.18 Colorless TMB was oxidizedby ROS generated from photosensitization and resultedin a blue-colored product, which was examined by an absorbanceat 655 nm. A mixture of TMB (200 g¢mL1) in a citricacid-sodium acetate buffer (0.1 M, pH 4) and test material(2 g¢mL1) was diluted with water (2 mL). Then, the mixturewas irradiated under an LED light (450 nm, 0.08Wcm2,SkyFire (LumiTorch), Taiwan), and the oxidation of TMBmolecules was quantified by monitoring an absorbance (at 655nm) of the irradiated mixture with a UVvisibleabsorptionspectrophotometer.
With molybdenum(IV) disulfide; dihydrogen peroxide In ethanol at 40℃; for 0.666667h;
With dihydrogen peroxide In water
With dihydrogen peroxide In aq. acetate buffer
With dihydrogen peroxide In aq. acetate buffer at 20℃; Flow reactor;
With dihydrogen peroxide In aq. acetate buffer at 35℃; for 0.25h;
With dihydrogen peroxide
With dihydrogen peroxide In aq. acetate buffer at 45℃; for 0.166667h;
With dihydrogen peroxide In aq. phosphate buffer; dimethyl sulfoxide at 20℃; for 0.0833333h; UV-irradiation;
With dihydrogen peroxide In aq. acetate buffer at 45℃; for 0.833333h; Colorimetric detection of H2O2 1.0 mL of HAc-NaAc buffer solutions (0.2 mol/L, pH 4.0) containing 0.2 mmol/L TMB, 0.4 μg/mL FeS NSs and various concentrations of H2O2(0, 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, 90, 100, 125, 150, 200, 250, 300, 400, 500, 600, 800, 1000 μmol/L) was prepared in order to explore the linearity of detecting H2O2. The mixed solutions were respectively incubated at 45oC for 50 min and then their absorbances at 652 nm were recorded for quantitative detection of H2O2. The limit of detection(LOD)was calculated from the linear calibration plots for the detection of H2O2in the range from 0 to 150 μmol/L with the formula (3σ/slope) at the signal to noise ratio (S/N = 3).
With platinum(IV) coordinate carbon dot-titanium dioxide hetrojunction Sonication; (2) Pt(IV)-CDTiO2-x prepared in Example 4 of the present invention. The sonosensitizer can generate a large amount of hydroxyl radicals(OH) under low-intensity ultrasound, and the detection of Pt(IV)-CDTiO2-x·OH by using 3,3,5,5-tetramethylbenzidine (TMB)generation efficiency of sonosensitizers under ultrasonic irradiation.Pt(IV)-CDTiO2-x prepared in Example 4 of the present application of the heterojunction. The detection results of the generation efficiency of 1O2 and OH are shown in Figure 2.It can be seen from Figure 2 that Pt(IV)-CDTiO2-x of the heterojunction1O2and OH production rates are better than those of TiO2-xNanosheets and TiO2Nanosheets.
With cerium coordinated 2,6-pyridinedicarboxylic acid with guanosine monophosphate polymer for 0.0166667h; 2.4. Steady-State kinetic analysis of DPA-Ce-GMP CPNs as oxidasemimetics General procedure: Kinetic experiments were implemented according to a report (Ding,Wang, Sun, & Lin, 2018). 0.1 mg/mL of DPA-Ce-GMP and 0.6 mM ofTMB were dropped into Tris-HCl (pH = 6.5). The mixture solutions wereincubated for 1 min and the absorbance change at 652 nm was monitored.The Michaelis-Menten constant was calculated by using aLineweaver-Burk plot: 1/V = Km/Vmax(1/[S] + 1/Km), where V is theinitial velocity, Vmax is the maximal reaction velocity, [S] is the substrateconcentration, and Km is the Michaelis-Menten constant.
With dihydrogen peroxide
With dihydrogen peroxide In ethanol at 45℃; Evaluation of the catalytic activity of Cu-MOF The peroxidase-like activity of Cu-MOF was carried out bymonitoring the oxidation of TMB (peroxidase substrate) inthe presence of H2O2at 652 nm using a UV-Vis spectrophotometer.In an assay reaction (500 μL), 250 mM sodiumacetate (NaOAC) buffer (pH 5.5) (390 μL), 1.875 mM TMB(40 μL, ethanol solution), and 0.154 mM H2O2(20 μL) wereadded into 50 μL of 0.1 mg/mL Cu-MOF aqueous solution.After incubation at 45 °C for 15 min, the color change andthe absorption spectra at 652 nm were measured. Somecontrol samples, including the solution of H2O2and Cu-MOF, the solution of TMB in the presence of H2O2,andthe solution of TMB in the presence of Cu-MOF, were alsoincubated at the same condition. After 15 min of incubation,the absorption spectra of these samples were obtainedat 652 nm [47].
With dihydrogen peroxide In aq. acetate buffer
With dihydrogen peroxide In aq. acetate buffer at 25℃;
With Fe-P/N-C single-atom nanoenzyme In aq. acetate buffer at 35℃; 2.3. Oxidase-like activity of the Fe-P/N-C The oxidase-like activity of Fe-P/N-C was evaluated through catalyzing oxidation of TMB, ABTS and OPD as a chromogenic substrate. Specifically, 50 μL of Fe-P/N-C (0.16 mg/L), 50 μL of the substrate (4 mm) and 100 μL of NaAC-HAC buffer (0.2 M, pH 4.0) were mixed andt he absorption spectra were measured by UV-vis spectroscopy after reaction at 37°C for 20 min.
With dihydrogen peroxide In aq. phosphate buffer Mimetic Peroxidase Activity he mimetic peroxidase activitiesof the Fe,N-UHCF were measured in 3 mL of 200 mMdisodium hydrogen phosphate-100 mM citric acid buffer (pH=5.0). H2O2(10 mM) and TMB (0.5 mM) were used as the substrate of the reaction, and Fe,N-UHCF (1mgml-1) was used as the catalysts of the reaction. The change of the absorbance under time-drive mode of the reaction system at 652 nm was monitored by a Cary 300 UV-Vis spectrophotometer (Varian, USA).
With cobalt(III) oxide; dihydrogen peroxide In water
With Co(benzene-1,4-dicarboxylate)(1,4-diazabicyclo[2.2.2]octane)0.5; oxone In dimethyl sulfoxide at 20℃;
With dihydrogen peroxide In aq. acetate buffer at 20℃;
With dihydrogen peroxide In aq. acetate buffer
With dihydrogen peroxide In water UV-irradiation;
With 1,3-diphenylisobenzofuran; dihydrogen peroxide In ethanol
With dihydrogen peroxide at 20℃; Microbiological reaction;
With dihydrogen peroxide In aq. acetate buffer at 55℃;

References: [1]Kireyko; Veselova; Shekhovtsova [Russian Journal of Bioorganic Chemistry, 2006, vol. 32, # 1, p. 71 - 77].
[2]Li, Runqing; Liu, Jingquan; Li, Luhua; Wang, Hongbin; Weng, Ziqing; Lam, Simon K.H.; Du, Aijun; Chen, Ying; Barrow, Colin J.; Yang, Wenrong [Chemical Communications, 2014, vol. 50, # 2, p. 225 - 227].
[3]Current Patent Assignee: UNIVERSITY OF SHEFFIELD - WO2015/33120, 2015, A1 Location in patent: Sheet 1/11.
[4]Cai, Ren; Yang, Dan; Peng, Shengjie; Chen, Xigao; Huang, Yun; Liu, Yuan; Hou, Weijia; Yang, Shengyuan; Liu, Zhenbao; Tan, Weihong [Journal of the American Chemical Society, 2015, vol. 137, # 43, p. 13957 - 13963].
[5]Sahoo, Ramkrishna; Santra, Sumita; Ray, Chaiti; Pal, Anjali; Negishi, Yuichi; Ray, Samit Kumar; Pal, Tarasankar [New Journal of Chemistry, 2016, vol. 40, # 2, p. 1861 - 1871].
[6]Roy, Anindita; Sahoo, Ramkrishna; Ray, Chaiti; Dutta, Soumen; Pal, Tarasankar [RSC Advances, 2016, vol. 6, # 38, p. 32308 - 32318].
[7]Cai, Ren; Yang, Dan; Chen, Xigao; Huang, Yun; Lyu, Yifan; He, Jinglin; Shi, Muling; Teng, I-Ting; Wan, Shuo; Hou, Weijia; Tan, Weihong [Journal of Materials Chemistry B, 2016, vol. 4, # 27, p. 4657 - 4661].
[8]Pal, Jaya; Pal, Tarasankar [RSC Advances, 2016, vol. 6, # 87, p. 83738 - 83747].
[9]Yang, Hankun; Xiao, Jingyu; Su, Lei; Feng, Ting; Lv, Qingye; Zhang, Xueji [Chemical Communications, 2017, vol. 53, # 27, p. 3882 - 3885].
[10]Li, Meng; Huang, Xiang-Rong; Guo, Yi; Shang, Ya-Zhuo; Liu, Hong-Lai [Chinese Chemical Letters, 2017, vol. 28, # 7, p. 1453 - 1459].
[11]Guo, Yuehua; Chen, Jielin; Cheng, Mingpan; Monchaud, David; Zhou, Jun; Ju, Huangxian [Angewandte Chemie - International Edition, 2017, vol. 56, # 52, p. 16636 - 16640][Angew. Chem., 2017, vol. 129, # 52, p. 16863 - 16867,5].
[12]Lach, Marcel; Künzle, Matthias; Beck, Tobias [Chemistry - A European Journal, 2017, vol. 23, # 69, p. 17482 - 17486].
[13]Gao, Yan; Jin, Chunqiao; Chen, Miaomiao; Zhu, Xixi; Fu, Min; Liu, Zhenxue; Gao, Linna; Liu, Qingyun [Journal of Porphyrins and Phthalocyanines, 2018, vol. 22, # 9-10, p. 935 - 943].
[14]Borthakur, Priyakshree; Boruah, Purna K.; Das, Manash R.; Szunerits, Sabine; Boukherroub, Rabah [New Journal of Chemistry, 2019, vol. 43, # 3, p. 1404 - 1414].
[15]Wang, Xin; Lv, Wenxin; Wu, Jiahui; Li, Haiyin; Li, Feng [Chemical Communications, 2020, vol. 56, # 33, p. 4571 - 4574].
[16]Sheng, Enze; Lu, Yuxiao; Tan, Yuting; Xiao, Yue; Li, Zhenxi; Dai, Zhihui [Food Chemistry, 2020, vol. 331].
[17]Chishti, Benazir; Fouad; Seo; Alothman, Othman Y.; Ansari [New Journal of Chemistry, 2020, vol. 44, # 26, p. 11291 - 11303].
[18]Sun, Jing; Wang, Rui; Xia, Meng; Zhu, Shuyun; Zhao, Xian-En [New Journal of Chemistry, 2020, vol. 44, # 30, p. 12962 - 12966].
[19]Bhattacharyya, Soumalya; Ali, Sk Rajab; Venkateswarulu, Mangili; Howlader, Prodip; Zangrando, Ennio; De, Mrinmoy; Mukherjee, Partha Sarathi [Journal of the American Chemical Society, 2020, vol. 142, # 44, p. 18981 - 18989].
[20]Tian, Xue; Qi, Wenjing; Zhao, Maoyu; Lai, Jianping; Wu, Di; Hu, Lianzhe; Zhang, Yan [New Journal of Chemistry, 2020, vol. 44, # 48, p. 21176 - 21182].
[21]Han, Shuai; Chen, Xiaohan; Fan, Yifei; Zhang, Yuexing; Yang, Zhongdong; Kong, Xia; Liu, Zhenxue; Liu, Qingyun; Zhang, Xianxi [New Journal of Chemistry, 2021, vol. 45, # 4, p. 2030 - 2037].
[22]Chen, Wei; Liu, Chuang; Ji, Xiaoyuan; Joseph, John; Tang, Zhongmin; Ouyang, Jiang; Xiao, Yufen; Kong, Na; Joshi, Nitin; Farokhzad, Omid C.; Tao, Wei; Xie, Tian [Angewandte Chemie - International Edition, 2021, vol. 60, # 13, p. 7155 - 7164][Angew. Chem., 2021, vol. 133, # 13, p. 7231 - 7240,10].
[23]Ma, Shenqian; Zhao, Weixin; Zhou, Jun; Wang, Jiaou; Chu, Shengqi; Liu, Zigeng; Xiang, Guolei [Chemical Science, 2021, vol. 12, # 12, p. 4411 - 4417].
[24]Dan, Xu; Ruiyi, Li; Qinsheng, Wang; Yongqiang, Yang; Haiyan, Zhu; Zaijun, Li [New Journal of Chemistry, 2021, vol. 45, # 16, p. 7129 - 7137].
[25]Do, Thu Thi Anh; Imae, Toyoko [Bulletin of the Chemical Society of Japan, 2021, vol. 94, # 8, p. 2079 - 2088].
[26]Shi, Rui; He, Qiaoling; Cheng, Shiqi; Chen, Bolin; Wang, Yilin [New Journal of Chemistry, 2021, vol. 45, # 38, p. 18048 - 18053].
[27]Niu, Jingsheng; Zhao, Chuanqi; Liu, Chun; Ren, Jinsong; Qu, Xiaogang [Chemistry of Materials, 2021, vol. 33, # 20, p. 8052 - 8058].
[28]Yang, Zhaopu; Fu, Xinliang; Ma, Daichuan; Wang, Yulin; Peng, Liming; Shi, Jiacheng; Sun, Jiyu; Gan, Xueqi; Deng, Yi; Yang, Weizhong [Small, 2021, vol. 17, # 50].
[29]Lin, Geyu; Cai, Jiandong; Sun, Yan; Cui, Yan; Liu, Qiuwen; Manners, Ian; Qiu, Huibin [Angewandte Chemie - International Edition, 2021, vol. 60, # 46, p. 24637 - 24643][Angew. Chem., 2021, vol. 133, # 46, p. 24842 - 24848].
[30]Yang, Liting; Jin, Ziqi; Zheng, Jing; Zhang, Baishun; Xu, Jingli; Yin, Xue-Bo; Zhang, Min [Inorganic Chemistry, 2022, vol. 61, # 1, p. 542 - 553].
[31]Xiao, Jiayu; Hai, Luo; Li, Yaoyao; Li, Huan; Gong, Minhui; Wang, Zefeng; Tang, Zifeng; Deng, Le; He, Dinggeng [Small, 2022, vol. 18, # 9].
[32]Feng, Xiaoyang; Fu, Hao; Bai, Zhenyu; Li, Ping; Song, Xingliang; Hu, Xueping [New Journal of Chemistry, 2022, vol. 46, # 1, p. 239 - 249].
[33]Chen, Lijuan; Li, Xiang; Li, Zezhi; Liu, Kejian; Xie, Jianping [RSC Advances, 2021, vol. 12, # 1, p. 595 - 601].
[34]Duan, Yefan; Li, Qi; He, Panpan; Li, Yan; Song, Jingrun; Wang, Jing; Liu, Junjie; Zhou, Jiang; Chen, Fei; Huang, Zhusheng; Sun, Jianfei; Zhang, Ying; Luo, Zhimin [Chinese Chemical Letters, 2022, vol. 33, # 6, p. 3217 - 3220].
[35]Current Patent Assignee: SHANGHAI SIXTH PEOPLE S HOSPITAL - CN113648414, 2021, A Location in patent: Paragraph 0028; 0054; 0055.
[36]Wang, Jianan; Wang, Xueyang; Wang, Min; Bian, Qinghua; Zhong, Jiangchun [Food Chemistry, 2022, vol. 397].
[37]Hou, Linqian; Gong, Fei; Han, Zhihui; Wang, Yuanjie; Yang, Yuqi; Cheng, Shuning; Yang, Nailin; Liu, Zhuang; Cheng, Liang [Angewandte Chemie - International Edition, 2022, vol. 61, # 39][Angew. Chem., 2022, vol. 134, # 39].
[38]Shahba, Arezoo; Karami, Zahra; Mirzaiebadizi, Amin; Badoei-dalfard, Arastoo [Journal of the Iranian Chemical Society, 2023, vol. 20, # 3, p. 563 - 576].
[39]Chang, Mengyu; Hou, Zhiyao; Wang, Man; Wen, Ding; Li, Chunxia; Liu, Yuhui; Zhao, Yanli; Lin, Jun [Angewandte Chemie - International Edition, 2022, vol. 61, # 50][Angew. Chem., 2022, vol. 134, # 50].
[40]Li, Huiqin; Wu, Hongjiao; Chen, Jiaqi; Su, Yiqian; Lin, Pengcheng; Xiao, Wei; Cao, Donglin [Langmuir, 2022, vol. 38, # 50, p. 15559 - 15569].
[41]Li, Ying; Javed, Rida; Li, Rui; Zhang, Yuyang; Lang, Ziyue; Zhao, Hongbin; Liu, Xing; Cao, Hongmei; Ye, Daixin [Food Chemistry, 2023, vol. 406].
[42]Hao, Jinyu; Zhang, Cui; Feng, Chenxi; Wang, Qian; Liu, Zhong-Yi; Li, Yan; Mu, Jianshuai; Yang, En-Cui; Wang, Yan [Chinese Chemical Letters, 2023, vol. 34, # 3].
[43]Wang, Zuochao; Liu, Jiao; Zhao, Huan; Xu, Wenxia; Liu, Jiaxin; Liu, Ziyi; Lai, Jianping; Wang, Lei [Chemical Science, 2023, vol. 14, # 7, p. 1878 - 1884].
[44]Deng, Zehui; Xiao, Qingling; Fu, Heyun; Zheng, Shourong; Alvarez, Pedro J.J.; Zhu, Dongqiang; Xu, Zhaoyi; Qu, Xiaolei [Chemical Communications, 2023, vol. 59, # 22, p. 3277 - 3280].
[45]Chen, Xiaofang; Wang, Ya; Feng, Min; Deng, Die; Xie, Xiaoyi; Deng, Caixia; Khattak, Kashif Nawaz; Yang, Xiupei [Chinese Chemical Letters, 2023, vol. 34, # 6].
[46]Wang, Longwei; Zhang, Xiaodi; You, Zhen; Yang, Zhongwei; Guo, Mengyu; Guo, Jiawei; Liu, He; Zhang, Xiaoyu; Wang, Zhuo; Wang, Aizhu; Lv, Yawei; Zhang, Jian; Yu, Xin; Liu, Jing; Chen, Chunying [Angewandte Chemie - International Edition, 2023, vol. 62, # 11][Angew. Chem., 2023, vol. 135, # 11].
[47]Duan, Yanqiu; Yu, Yang; Liu, Peilai; Gao, Yao; Dai, Xinyue; Zhang, Liang; Chen, Liang; Chen, Yu [Angewandte Chemie - International Edition, 2023, vol. 62, # 20][Angew. Chem., 2023, vol. 135, # 20].
[48]Wang, Hao; Bao, Wenxin; Sarwar, Muhammad Tariq; Tian, Luyuan; Tang, Aidong; Yang, Huaming [Inorganic Chemistry, 2023, vol. 62, # 21, p. 8418 - 8427].
[49]Xing, Gaowa; Shang, Yuting; Ai, Jiebing; Lin, Haifeng; Wu, Zengnan; Zhang, Qiang; Lin, Jin-Ming; Pu, Qiaosheng; Lin, Ling [Analytical Chemistry, 2023, vol. 95, # 35, p. 13391 - 13399].
[50]Shen, Ying; Wu, Hongyuan; Luo, Xia; Zhang, Haizhi; Cheng, Liming [RSC Advances, 2023, vol. 13, # 39, p. 27283 - 27291].
  • 6
  • [ 540-80-7 ]
  • [ 54827-17-7 ]
  • [ 144653-01-0 ]
YieldReaction ConditionsOperation in experiment
43% In Bromoform; ethyl acetate 4.a a) a) Preparation of 4,4'-dibromo-3,3',5,5'-tetramethyl biphenyl 32 g (312 mmoles) of t-BuONO and 75 ml of bromoform are placed in a 500 ml two-neck flask fitted with a mechanical stirrer, condenser, dropping funnel and heating bath. 25 g (104 mmoles) of 3,3',5,5'-tetramethylbenzidine dissolved in 175 ml of bromoform are added to the mixture heated in an inert atmosphere to 65° C. under stirring. The resultant mixture is left stirring at 65° C. for 2 hours and is then filtered. The precipitate is washed with ethyl ether, the used wash liquid together with the filtrate then being evaporated to dryness. The residue is then dissolved in 70 ml of ethyl acetate and chromatographed in a silica gel column of 2.5 litres volume, eluding with petroleum ether. A single fraction having a volume of 6 liters is recovered, from which 31 g of still crude product are recovered after evaporation. Repetition of the purification procedure under the stated conditions leads to the recovery of 16.5 g of the desired product, with a molar yield of 43%. Analysis: TLC-Silica F (petroleum ether/ethyl acetate 75:25). 1 H-NMR (DMSO-D6): δ 2.42 (s, 12H, CH3); 7.51 (s, 2H, aromatic) IR and MS in accordance with the structure.
43% In Bromoform; ethyl acetate 4.a a) a) Preparation of 4,4'-dibromo-3,3',5,5'-tetramethyl biphenyl 32 g (312 mmoles) of t-BuONO and 75 ml of bromoform are placed in a 500 ml two-neck flask fitted with a mechanical stirrer, condenser, dropping funnel and heating bath. 25 g (104 mmoles) of 3,3',5,5'-tetramethylbenzidine dissolved in 175 ml of bromoform are added to the mixture heated in an inert atmosphere to 65°C under stirring. The resultant mixture is left stirring at 65°C for 2 hours and is then filtered. The precipitate is washed with ethyl ether, the used wash liquid together with the filtrate then being evaporated to dryness. The residue is then dissolved in 70 ml of ethyl acetate and chromatographed in a silica gel column of 2.5 litres volume, eluding with petroleum ether. A single fraction having a volume of 6 litres is recovered, from which 31 g of still crude product are recovered after evaporation. Repetition of the purification procedure under the stated conditions leads to the recovery of 16.5 g of the desired product, with a molar yield of 43%.
  • 7
  • [ 24596-19-8 ]
  • [ 54827-17-7 ]
  • [ 87-62-7 ]
YieldReaction ConditionsOperation in experiment
7.7 parts (63.3%) With sodium formate; cetyltrimethylammonim bromide In water 8 EXAMPLE 8 EXAMPLE 8 4-Bromo-2,6-dimethylaniline (24.0 parts), 3% palladium on charcoal (50% paste; 2.0 parts), cetyltrimethylammonium bromide (4.0 parts), sodium hydroxide liquor (32%; 13.5 parts), sodium formate (6.8 parts) in water (60 parts) are stirred at the boil under reflux for 3 hours. Sodium formate (6.8 parts is then added and the mixture boiled for 3 hours when a further charge of sodium formate (6.8 parts) is made. The reaction mixture is then held at the boil under reflux for a further 23 hours. The mixture is then steam distilled and from the distillate 2.2 parts (18.2%) of 2,6-dimethylaniline is recovered by extraction with ether. The reaction mixture after steam distillation is cooled to room temperature and then filtered. The residue is extracted continuously with methanol (60 parts) for 5 hours and then the extract cooled. The colourless crystalline precipitate is filtered off and dried to give 7.7 parts (63.3%) of 3,3',5,5'-tetramethyl benzidine.
  • 8
  • [ 54827-17-7 ]
  • [ 134296-07-4 ]
  • [ CAS Unavailable ]
YieldReaction ConditionsOperation in experiment
34% In diethyl ether for 48h; Heating / reflux; 24 To a suspension of 2,2'-bipyridinyl-6-carbaldehyde (0.39 g, 2.10 mmol) in diethyl ether (5 ml) was added la (0.13 g, 0.53 mmol, 0.25 eq. ) and one drop of formic acid. The yellow reaction mixture was heated to reflux for 48 hours. On cooling to room temperature the suspension was filtered, washed with cold diethyl ether and dried under reduced pressure to give 15 as a yellow solid (0.10 g, 34%). Compound 15: ES mass spectrum, m/z 573 [M+H]+. ¹H NMR (CDCl3), δ 2.18 (s, 12H, (Ar-CH3)), 7.29 (s, 4H, Ar-H), 7.41 (dd, 2H, 3J(HH) 6.7, 4J(HH) , Py-H), 7.75 (td, 2H, 3J(HH) 7.8, 4J(HH) 1.8, Py-H), 7.90 (t, 2H, 3J(HH) 7.8, Py-H), 8.27 (dd, 2H, 3J(HH) 7.8, 4J (HH) 1.6, Py-H), 8.40 (s, 2H, HC=N), 8.44 (dd, 2H, 3J(HH) 7.5, 4J(HH) 1.2, Py- H), 8.46 (dd, 2H, 3J(HH) 7.4, 4J(HH) 0.9, Py-H), 8.62 (dd, 2H, 3J(HH) 4.3, 4J(HH) 0.7, Py-H).
  • 9
  • [ 1121-60-4 ]
  • [ 54827-17-7 ]
  • [ CAS Unavailable ]
YieldReaction ConditionsOperation in experiment
62% In ethanol Heating / reflux; 20 To a suspension of 1a (0.48 g, 2.00 mmol) in absolute ethanol (10 ml) was added 2-pyridinecarboxaldehyde (0.67 ml, 7.00 mmol, 3.5 eq. ). The mixture was stirred and heated to reflux overnight: On cooling to room temperature, the suspension was filtered, washed with cold ethanol and dried under reduced pressure to give 13a in good yield as a yellow solid (0.51 g, 62%). Compound 13a: ES mass spectrum, m/z 419 [M+H] (at); IR (cm-1) 1648,1584, 1566 (C=N) ; ¹H NMR (CDCl3), δ 2.20 (s, 12H, (Ar-CH3)), 7.25 (s, 4H, Ar-H), 7.3-7.4 (m, 2H, Py-H), 7.7-7.9 (m, 2H, Py-H), 8.2-8.4 (m, 2H, Py-H), 8.35 (s, 2H, CH=N), 8.7-8.8 (m, 2H, Py-H); ¹3C NMR (CDCl3, ¹H gated decoupled), δ 18.9 (s, Me), 121.7 (s, Ar), 125.8 (s, Ar), 127,1 (s, Ar), 127.8 (s, Ar), 137.2 (s, Ar), 137.3 (s, Ar), 149.7 (s, Ar), 150.1 (s, Ar), 157.3 (s, Ar), 164.0 (s, C=N). Anal. (C2sH26N4) calcd: C, 80.34; H, 6.27; N, 13.38. Found: C, 80.15; H, 6.35; N, 13.32%. In addition, a single crystal X-ray diffraction study of 13a has confirmed the structural type.
  • 10
  • [ 1122-62-9 ]
  • [ 54827-17-7 ]
  • [ CAS Unavailable ]
YieldReaction ConditionsOperation in experiment
41% In ethanol Heating / reflux; 21 To a suspension of 1a (0.50 g, 2.10 mmol) in absolute ethanol (10 ml) was added 2-acetylpyridine (0.80 ml, 7.10 mmol, 3.4 eq. ) and two drops of formic acid. The suspension was heated to reflux overnight. On cooling to room temperature the suspension was filtered, washed with cold ethanol and dried under reduced pressure to give 13b as a yellow solid (0.51 g, 41%). Compound 13b: ES mass spectrum, m/z 447 [M+H] +; ¹H NMR (CDCl3), δ 2.04 (s, 12H, Ar-CH3), 2.19 (s, 6H, (CH3)C=N), 7.26 (s, 4H, Ar-H), 7.3-7.4 (m, 2H, Py-H), 7.7- 7.8 (m, 2H, Py-H), 8.3-8.4 (m, 2H, Py-H), 8.6-8.7 (m, 2H, Py-H) ; ¹3C NMR (CDCl3, ¹H gated decoupled), δ 17.2(s, Ar-CH3), 18.5 (s, (CH3) C=N), 121.8 (s, Ar), 125.3 (s, Ar), 126.2 (s, Ar), 126.8 (s, Ar), 136.4 (s, Ar), 136.9 (s, Ar), 148.0 (s, Ar), 149.0 (s, Ar), 156.9 (s, Ar), 167.9 (s, C=N).
  • 11
  • [ 868961-34-6 ]
  • [ 54827-17-7 ]
  • [ 1071505-64-0 ]
YieldReaction ConditionsOperation in experiment
96% In tetrahydrofuran
  • 12
  • [ 54827-17-7 ]
  • [ 1140509-33-6 ]
YieldReaction ConditionsOperation in experiment
43% Stage #1: 3,3',5,5'-tetramethylbenzidine With hydrogenchloride; sodium nitrite In water at 0℃; for 0.75h; Stage #2: With potassium iodide In dichloromethane; water at 0 - 20℃; for 4h;
24% Stage #1: 3,3',5,5'-tetramethylbenzidine With sulfuric acid In water; acetone; toluene Inert atmosphere; Stage #2: With sodium nitrite In water; acetone; toluene at 0℃; for 3h; Inert atmosphere; Stage #3: With potassium iodide In water; acetone; toluene at 40 - 80℃; Inert atmosphere;
Stage #1: 3,3',5,5'-tetramethylbenzidine With hydrogenchloride; sodium nitrite In water at 0℃; for 0.25h; Stage #2: With potassium iodide In water at 0 - 20℃; I.1.d.1 1st Step:; 10.2 g of tetramethylbenzidine (98%, Alfa Aesar) are suspended in 39 ml of concentrated hydrochloric acid (37%, sold by the company Aldrich) at 0° C. The diazotization was performed by adding a sodium nitrite solution (6 g in 50 ml of water). After stirring for 15 minutes at 0° C., a potassium iodide solution (70 g in 200 ml of water) was added slowly to the resulting violet solution. Once the addition is complete, the mixture is stirred for 2 hours at room temperature. The resulting black suspension is filtered to recover a black precipitate, which is washed with water. The solid is suspended in dichloromethane (DCM, 98%, sold by the company SDS) and a saturated sodium thiosulfate solution is added, causing decolorization. After stirring for 1 hour, the organic phase is separated out by settling and the aqueous phase is extracted with DCM. The organic phase is dried over sodium sulfate and then evaporated to give the diiodo intermediate in the form of a grayish solid. Elution with pure pentane on a column of silica (sold by the company SDS) gives a mixture of the monoiodo and diiodo compounds. The mixture of these compounds was used directly in the following step.
Stage #1: 3,3',5,5'-tetramethylbenzidine With hydrogenchloride; sodium nitrite In water at 0℃; for 0.75h; Stage #2: With potassium iodide In dichloromethane; water at 20℃; for 4h; Cooling with ice; Synthesis of F: A solution of NaNO2 (2.32 g) in 20 mL water was added to a cloudy mixture of E (6.6 g, 27.8 mmol) in 30 mL2M hydrochloric acid at 0 °C. After stirred at 0 °C for 45 minutes, an ice-cold KI aqueous solution was added. Thenmixture changed to dark red and sticky. After 100 mL CH2Cl2 was added, the mixture was allowed to stir at RT for 4hours. The aqueous phase was washed with CH2Cl2 three times. The combined organic phases were dried with MgSO4.After the solvent was removed, the crude product was purified by column chromatography with Ethyl acetate: Hexane= 4: 1 as the eluent. 1H NMR (CDCl3): δ = 2.538 (s, 12H), 7.261 (s, 4H).
Stage #1: 3,3',5,5'-tetramethylbenzidine With hydrogenchloride; sodium nitrite In water at 0℃; for 0.75h; Stage #2: With potassium iodide In dichloromethane; water at 0 - 20℃; for 4h; Synthesis ofF: A solution of NaN02 (2.32 g) in 20 mL water was added to a cloudy mixture of E (6.6 g, 27.8 mmol) in 30 mL 2M hydrochloric acid at o °C. After stirred at 0 °C for 45 minutes, an ice-cold KI aqueous solution was added. Then mixture changed to dark red and sticky. After 100 mL CH2C12 was added, the mixture was allowed to stir at RT for 4 hours. The aqueous phase was washed with CH2C12 three times. The combined organic phases were dried with MgS04. After the solvent was removed, the crude product was purified by column chromatography with Ethyl acetate: Hexans = 4: 1 as the eluent. NMR (CDC13): δ = 2.538 (s, 12H), 7.261 (s, 4H).
Stage #1: 3,3',5,5'-tetramethylbenzidine With hydrogenchloride; sodium nitrite In water at 0℃; for 0.75h; Stage #2: With potassium iodide In water at 0 - 20℃; for 4h; Synthesis of F: A solution of NaNO2 (2.32 g) in 20 mE water was added to a cloudy mixture of E (6.6 g, 27.8 mmol) in 30 mE 2M hydrochloric acid at 0° C. After stirred at 0° C. for 45 minutes, an ice-cold KI aqueous solution was added. Then mixture changed to dark red and sticky. After 100 mE CH2C12 was added, the mixture was allowed to stir at RT for 4 hours. The aqueous phase was washed with CH2C12 three times. The combined organic phases were dried with Mg504. Afier the solvent was removed, the crude product was purified by column chromatography with Ethyl acetate:Hexane=4: 1 as the eluent. ‘H NMR (CDC13): ö=2.538 (s, 12H), 7.261 (s, 4H).

  • 13
  • [ 24596-18-7 ]
  • [ 54827-17-7 ]
YieldReaction ConditionsOperation in experiment
93% With carbon dioxide; aluminium; 1-butyl-3-methylimidazolium trifluoromethanesulfonimide at 45℃; for 10h;
91% With 4-(3'-butyl-1'-imidazolio)-1-butanesulfonic acid hydrogen sulfate; aluminium In carbon dioxide at 45℃; for 10h; Supercritical conditions;
  • 14
  • [ 54827-17-7 ]
  • [ 541-41-3 ]
  • [ 1189123-39-4 ]
YieldReaction ConditionsOperation in experiment
Stage #1: 3,3',5,5'-tetramethylbenzidine With hydrogenchloride; sodium nitrite In water at 0℃; for 0.25h; Stage #2: With potassium iodide In water at 20℃; Stage #3: chloroformic acid ethyl ester 2.b.1+2 Step 1: 10.2 g of tetramethylbenzidine (98%, Alfa Aesar) are suspended in 39 ml of concentrated hydrochloric acid (37%, marketed by Aldrich) at 0° C. The diazotization was effected by addition of a solution of sodium nitrite (6 g in 50 ml of water). After stirring for 15 minutes at 0° C., a solution of potassium iodide (70 g in 200 ml of water) was slowly added to the resulting violet solution. On completion of the addition, the mixture is stirred for 2 hours at ambient temperature. The resulting black suspension is filtered to recover a black precipitate, which is washed in water. The solid is suspended in dichloromethane (DCM, 98%, marketed by SDS) and a saturated solution of sodium thiosulfate is added, causing decolorization. After stirring for 1 hour, the organic phase is decanted and the aqueous phase is extracted with DCM. The organic phase is dried over sodium sulfate, then evaporated to give the diiodo intermediate in the form of a grayish solid. Elution with pure pentane on a silica column (marketed by SDS) yields the mixture of monoiodo and diiodo compounds. The mixture of these was used directly in the following step.2. Step 2: 7.2 g of the crude iodo compound is dissolved in 100 ml of tetrahydrofuran (THF, distilled over sodium). After cooling to -78° C., 35 ml of n-butyllithium in cyclohexane (2.5 M, marketed by Aldrich) is added. The solution is allowed to return to ambient temperature, a white suspension appearing after 2 hours. It is cooled again to -78° C. and 12 ml of ethyl chloroformate are added. The mixture is allowed to stand at ambient temperature, a clear yellow solution being obtained after 1 hour. Partition between water and dichloromethane, followed by extraction with dichloromethane gives the crude diester. This is purified by chromatography on silica gel, with a 1/9 Et2O/pentane mixture, (front retardation: Rf=0.3) as eluent. 6.3 g of diester are obtained in the form of a colorless solid (42% yield from benzidine).Characterization of diester obtained: 1H NMR (300 MHz, CDCl3): δ (ppm): 1.29 (t, J=7.2 Hz, 6H), 2.29 (s, 13H), 4.31 (q, J=7.2 Hz, 4H), 7.12 (s, 4H). 13C NMR (75 MHz, CDCl3): δ (ppm): 14.3 (CH3), 19.9 (CH3), 61.0 (CH2), 126.5 (CH), 133.2 (quadratic coupling), 135.5 (Quadratic coupling), 141.4 (Quadratic coupling), 169.8 (Quadratic coupling).
  • 15
  • [ 54827-17-7 ]
  • [ 130535-78-3 ]
YieldReaction ConditionsOperation in experiment
With dihydrogen peroxide; copper(II) oxide In dimethyl sulfoxide at 25℃;
With iron(III) protoporphyrin IX chloride; dihydrogen peroxide In aq. buffer
With 4Na(1+)*12C3H4N2*2C3H3N2(1-)*7Cu(2+)*31H2O*2H(1+)*2Bi(3+)*2W9O33(12-); dihydrogen peroxide In aq. acetate buffer at 55℃; for 0.166667h;
With dihydrogen peroxide; horseradish peroxidase In dimethyl sulfoxide at 25℃; Enzymatic reaction;
With dihydrogen peroxide; peroxidase Enzymatic reaction;
With dihydrogen peroxide In aq. acetate buffer at 40℃; for 0.666667h;
With dihydrogen peroxide In water at 30℃; for 0.0833333h;
With dihydrogen peroxide In aq. phosphate buffer for 0.166667h; Irradiation;
With Sodium orthovanadate; dihydrogen peroxide; iodine; Curvularia inaequalis vanadium dependent chloroperoxidase In aq. phosphate buffer at 25℃; for 0.5h; Enzymatic reaction;
With dihydrogen peroxide; AuPtPd In aq. acetate buffer at 37℃; for 0.25h;
With dihydrogen peroxide In aq. acetate buffer at 30℃;
With dihydrogen peroxide at 30℃;
Stage #1: 3,3',5,5'-tetramethylbenzidine With copper(II) ion; dihydrogen peroxide In aq. acetate buffer at 60℃; Stage #2: With sulfuric acid In aq. acetate buffer at 20℃; 2.6.2.2. Microplate protocol A mixture containing 40 μL of 75 mM xanthine, 65 μL of PBS (pH 7.8; v/v), and 20 μL of XO enzyme sample solution was added to each well of a 96 well plate in that order. Next, the plate was incubated at 37 °C for 30 min in a water bath. Then, 40 μL of 250 mM HA/SA buffer (pH 2,6), 20 μL of Cu2+, and 40 μL of TMB were added, in that order. The plate was mixed and incubated at 60 °C for 7min before being allowed to cool to room temperature. Finally, 25 μL of sulfuric acid (2 M) was added to each well, followed by thorough mixing. The absorbance was recorded at 450 nm.
Stage #1: 3,3',5,5'-tetramethylbenzidine With copper(II) ion; dihydrogen peroxide In aq. acetate buffer at 60℃; Stage #2: With sulfuric acid In aq. acetate buffer at 20℃; 2.6.2.2. Microplate protocol A mixture containing 40 μL of 75 mM xanthine, 65 μL of PBS (pH 7.8; v/v), and 20 μL of XO enzyme sample solution was added to each well of a 96 well plate in that order. Next, the plate was incubated at 37 °C for 30 min in a water bath. Then, 40 μL of 250 mM HA/SA buffer (pH 2,6), 20 μL of Cu2+, and 40 μL of TMB were added, in that order. The plate was mixed and incubated at 60 °C for 7min before being allowed to cool to room temperature. Finally, 25 μL of sulfuric acid (2 M) was added to each well, followed by thorough mixing. The absorbance was recorded at 450 nm.
With phosphotungstic acid covered with CeO2 hollow nanospheres In aq. acetate buffer at 20℃; 2.4. Colorimetric measurements The oxidase-like activity of CeO2PTA HNSs was first evaluated byusing TMB as a chromogenic substrate in acetate buffer. In a typicalexperiment, CeO2PTA HNSs (100 μL, 1 g/L) and TMB (50 μL, 10 mM)were injected into 850 μL HAc-NaOAc buffer (0.2 mM, pH 4.0) at roomtemperature for 30 min. Then, a UV-vis spectrophotometer was utilizedto record the absorption spectra (AbsTMB) at 652 nm.In order to quantificationally determine AA, 100 μL CeO2PTAHNSs were spiked with different concentrations of AA under the optimal condition initially. After incubation with TMB for 30 min, the absorbance(AbsAA) was measured by UV-vis at 652 nm. Afterward, the assayof AA was performed based on the calibration curve of the absorbancedifference (ΔAbs = AbsTMB- AbsAA) with AA concentration.
With dihydrogen peroxide In water; dimethyl sulfoxide
With dihydrogen peroxide In aq. buffer
With dihydrogen peroxide In aq. phosphate buffer at 37℃;
With dihydrogen peroxide
With dihydrogen peroxide In aq. acetate buffer at 35℃;
With dihydrogen peroxide In dimethyl sulfoxide at 20℃;
With dihydrogen peroxide In aq. acetate buffer at 40℃;
With dihydrogen peroxide In water at 25℃;
With Mn-based mesoporous 1,3,5-trimesic acid metal-organic framework at 0℃; Acidic conditions;
With manganese(IV) oxide
With β-cyclodextrin modified MnO2 nanosheets
With dihydrogen peroxide In aq. acetate buffer at 40℃; 3.2. Peroxidase-like and Oxidase-like Activity Measurement The catalytic oxidation of colorless TMB to blue oxTMB in the presence or absence of H2O2 was used to evaluate the peroxidase-like or oxidase-like activities of the FeCoNi-MOFand the other six materials for comparison. Acetate buffer (0.1M) with pH 4.0 was selectedas the matrix in the evaluation process [51]. Each of the seven materials was added tothe acetate buffer (pH 4.0) to form seven suspensions of 500 μg mL-1. Then, 50 μL ofthe suspension was dispersed into 850 μL acetate buffer (0.1 M, pH 4.0), to which 50 μLof H2O2 (10 mM) and 50 μL TMB (10 mM in DMF) were added. The absorbance of the mixture was recorded at 652 nm after it was incubated at 40 C for 30 min to complete athorough conversion of TMB to oxTMB. The material which had the highest peroxidaseactivity was chosen to construct the colorimetric sensor.
With horseradish peroxidase In aq. buffer Enzymatic reaction;
With copper 1,4-benzenedicarboxylate; dihydrogen peroxide In aq. phosphate buffer at 20℃;
With dihydrogen peroxide In aq. acetate buffer at 20℃;
With potassium chloride; dihydrogen peroxide UV-irradiation;
With hydroxyl Irradiation;
With dihydrogen peroxide In aq. phosphate buffer; dimethyl sulfoxide at 20℃; for 0.25h; 3.5. Assessment of Peroxidase-like Activity of β-CD-AuNPs The peroxidase-like activity of β-CD-AuNPs was evaluated by the classical chromogenicreaction of TMB and OPD in the presence of H2O2. In a typical assay, 2364 μLof an acetate buffer solution (0.01 M, pH 5.0), 12.0 μL of TMB (20 mM stock in DMSO),24.0 μL of H2O2, (1 M stock), and 100 μL of 6× concentrated AuNPs were sequentiallyadded directly in the cuvette. The reaction mixture was well mixed several times, and welet it react for fifteen minutes at room temperature. The absorbance spectra or absorbance at652 nm was then recorded using a Cary 50 UV-Vis spectrophotometer. For the kinetic assay,measurements were carried out in time course mode by monitoring the absorbance changeat 652 nm for TMB. The OPD assay also followed the same procedure except that TMB wasreplaced by OPD, the typical reaction time was 60 min, and absorbance was monitored at450 nm.
With Cu-doped-2-propylimidazole-modified nanoceria In aq. buffer at 20℃;
With dihydrogen peroxide In aq. acetate buffer Irradiation;
With dihydrogen peroxide
With sodium acetate; acetic acid In water Irradiation;

References: [1]Liu, Yuanjun; Zhu, Guoxing; Bao, Chunlin; Yuan, Aihua; Shen, Xiaoping [Chinese Journal of Chemistry, 2014, vol. 32, # 2, p. 151 - 156].
[2]Wu, Yuangen; Wang, Faze; Zhan, Shenshan; Liu, Le; Luo, Yanfang; Zhou, Pei [RSC Advances, 2013, vol. 3, # 48, p. 25614 - 25619].
[3]Chai, Dong-Feng; Ma, Zhuo; Yan, Hong; Qiu, YunFeng; Liu, Hong; Guo, Hua-Dong; Gao, Guang-Gang [RSC Advances, 2015, vol. 5, # 96, p. 78771 - 78779].
[4]Masud, Mostafa Kamal; Yadav, Sharda; Islam, Md. Nazmul; Nguyen, Nam-Trung; Salomon, Carlos; Kline, Richard; Alamri, Hatem R.; Alothman, Zeid A.; Yamauchi, Yusuke; Hossain, Md. Shahriar A.; Shiddiky, Muhammad J. A. [Analytical Chemistry, 2017, vol. 89, # 20, p. 11005 - 11013].
[5]Yetisen, Ali K.; Moreddu, Rosalia; Seifi, Sarah; Jiang, Nan; Vega, Katia; Dong, Xingchen; Dong, Jie; Butt, Haider; Jakobi, Martin; Elsner, Martin; Koch, Alexander W. [Angewandte Chemie - International Edition, 2019, vol. 58, # 31, p. 10506 - 10513][Angew. Chem., 2019, vol. 131, p. 10616 - 10623,8].
[6]Song, Chan; Ding, Wei; Liu, Haibo; Zhao, Weiwen; Yao, Yuewei; Yao, Cheng [New Journal of Chemistry, 2019, vol. 43, # 32, p. 12776 - 12784].
[7]Feng, Shiya; Ming, Mei; Wang, Mingzhu; Wang, Xue; He, Daiping; Jiang, Ping; Chen, Yuyun [Chemical Communications, 2020, vol. 56, # 82, p. 12347 - 12350].
[8]Liu, Yanhong; Shi, Qingyang; Zhang, Yan; Jing, Jili; Pei, Jin [New Journal of Chemistry, 2020, vol. 44, # 44, p. 19262 - 19269].
[9]Tang, Qingyun; Grathwol, Christoph W.; Aslan-Üzel, Aşkın S.; Wu, Shuke; Link, Andreas; Pavlidis, Ioannis V.; Badenhorst, Christoffel P. S.; Bornscheuer, Uwe T. [Angewandte Chemie - International Edition, 2021, vol. 60, # 3, p. 1524 - 1527][Angew. Chem., 2021, vol. 133, # 3, p. 1547 - 1551,5].
[10]Liu, Mingwang; Chen, Guojuan; Qin, Ying; Li, Jinli; Hu, Liuyong; Gu, Wenling; Zhu, Chengzhou [Analytical Chemistry, 2021, vol. 93, # 28, p. 9897 - 9903].
[11]Yu, Hao; Wu, Hanliu; Tian, Xuemei; Zhou, Yafen; Ren, Chunguang; Wang, Zhonghua [RSC Advances, 2021, vol. 11, # 43, p. 26963 - 26973].
[12]Xia, Shiyu; Wu, Fengxia; Cheng, Lu; Bao, Haibo; Gao, Wenping; Duan, Jin; Niu, Wenxin; Xu, Guobao [Small, 2023, vol. 19, # 6].
[13]Azeez, Ahlam Majid; Hadwan, Mahmoud Hussain [Analytical Biochemistry, 2023, vol. 673].
[14]Azeez, Ahlam Majid; Hadwan, Mahmoud Hussain [Analytical Biochemistry, 2023, vol. 673].
[15]Zhou, Tao; Zhang, Tonglei; Wang, Yan; Ge, Danhua; Chen, Xiaojun [Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2023, vol. 289].
[16]Chen, Xu; Yang, Yonglan; Ye, Gang; Liu, Shengming; Liu, Jie [Small, 2023, vol. 19, # 28].
[17]Dai, Xiaohui; Liu, Huan; Cai, Bin; Liu, Yang; Song, Kepeng; Chen, Jing; Ni, Shou-Qing; Kong, Lingshuai; Zhan, Jinhua [Small, 2023, vol. 19, # 47].
[18]Zhang, Yu; Cui, Tingting; Yang, Jie; Huang, Ying; Ren, Jinsong; Qu, Xiaogang [Angewandte Chemie - International Edition, 2023, vol. 62, # 34][Angew. Chem., 2023, vol. 135, # 34].
[19]Li, Wen; Qi, Manlin; Zhou, Jing; Sun, Yue; Sun, Jiao; Dong, Biao; Wang, Lin; Song, Shuyan [Small, 2024, vol. 20, # 19].
[20]Wang, Jian; Ye, Bo; Xiao, Shiqi; Liu, Xia [RSC Advances, 2024, vol. 14, # 4, p. 2697 - 2703].
[21]Zhang, Hao; Wang, Pengbo; Zhang, Jingru; Sun, Qingdi; He, Qian; He, Xiaohui; Chen, Hongyu; Ji, Hongbing [Angewandte Chemie - International Edition, 2024, vol. 63, # 9][Angew. Chem., 2024, vol. 136, # 9].
[22]Wang, Haiyan; Liu, Cheng; Zhao, Yue; Luo, Xinyu; Yin, Pengjie; Du, Fuyou; Zeng, Guangsheng [New Journal of Chemistry, 2024, vol. 48, # 11, p. 4886 - 4895].
[23]Bao, Wenxin; Tian, Luyuan; Wang, Hao; Tang, Aidong; Yang, Huaming [Inorganic Chemistry, 2024, vol. 63, # 7, p. 3366 - 3375].
[24]Miao, Yanming; Zhao, Xujuan; Sun, Xiaojie; Lv, Jinzhi [Food Chemistry, 2024, vol. 451].
[25]Huang, Ruiqi; Qin, Yingfeng; Huang, Yanqin; Huang, Zengqiong; Ye, Gao-Jie [Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2024, vol. 318].
[26]Huang, Ruiqi; Qin, Yingfeng; Huang, Yanqin; Huang, Zengqiong; Ye, Gao-Jie [Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2024, vol. 318].
[27]Deng, Zehui; Cao, Jiaqing; Zhao, Lei; Zhang, Zhao; Yuan, Jianwei [Molecules, 2024, vol. 29, # 16].
[28]Chen, Yifei; Wu, Yu; Xu, Weiqing; Tang, Yinjun; Cai, Yujia; Yu, Xin; Li, Jian; Qiu, Yiwei; Hu, Liuyong; Gu, Wenling; Zhu, Chengzhou [Analytical Chemistry, 2024].
[29]Singh, Ajit Kumar; Sharma, Deepika; Singh, Devesh Kumar; Sarraf, Sonu; Basu, Aviru Kumar; Ganesan, Vellaichamy; Saha, Avishek; Indra, Arindam [ChemCatChem, 2025, vol. 17, # 1].
[30]Hao, Jian; Shang, Rui; Shi, Miaotian; Yuan, Jincheng; Tan, Yi; Liu, Jiawei; Cai, Kai [Dalton Transactions, 2024, vol. 53, # 42, p. 17324 - 17332].
[31]Meng, Shuyun; Li, Yuye; Dong, Na; Liu, Shuda; Gong, Qingfa; Liu, Yifan; Zhang, Li; Niu, Qijian; Liu, Dong; You, Tianyan [Analytical Chemistry, 2024, vol. 96, # 43, p. 17217 - 17226].
[32]Xiang, Qinyanqiu; Yang, Xue; Zhang, Zhiqi; Yang, Jie; Li, Yingbo; Du, Jiawei; Wang, Jue; Fan, Kai; Yuan, Jiaxin; Zhang, Jianqiong; Xie, Jinbing; Ju, Shenghong [Advanced Materials, 2025, vol. 37, # 8].
[33]Anderson, Sara; Shepherd, Hamish; Boggavarapu, Kiran; Paudyal, Janak [Molecules, 2025, vol. 30, # 2].
[34]Fu, Zhendong; Qiu, Jiahe; Gong, Ping; Zhang, Danhong; Wang, Liping [RSC Advances, 2025, vol. 15, # 13, p. 9997 - 10004].
[35]Yuan, Simin; Ge, Lianyuan; Li, Yi; Wang, Xiaohong; Liu, Zhongyuan; Cao, Yang; Yang, Linglin [RSC Advances, 2025, vol. 15, # 13, p. 9985 - 9996].
[36]Zhang, Wencan; Shu, Jiangnan; Wang, Manli; Zheng, Keying; Cui, Hua [Analytical Chemistry, 2025, vol. 97, # 23, p. 12116 - 12124].
[37]Zhu, Chao; Gong, Chengtao; Cao, Duojun; Ma, Li-Li; Liu, Dongdong; Zhang, Liyan; Li, Yang; Peng, Yongwu; Yuan, Guozan [Angewandte Chemie - International Edition, 2025, vol. 64, # 24][Angew. Chem., 2025, vol. 64, # 24].
  • 16
  • [ 54827-17-7 ]
  • [ CAS Unavailable ]
YieldReaction ConditionsOperation in experiment
With vanadium dioxide; dihydrogen peroxide In aq. acetate buffer
With dihydrogen peroxide In ethanol at 20 - 70℃; for 0.5h; To evaluate the peroxidase-like catalytic activity of the RGO-INs, the catalytic oxidation of the peroxidase substrate TMB in the presence of H2O2 was tested. The measurements were carried out by monitoring the absorbance change of TMB at 652 nm. In a typical experiment, 80 μL of the RGO-INs dispersion (1 mg mL-1) was mixed in 1600 μL of NaAc buffer solution (pH 3.0), followed by adding 400 μL of TMB solution (1 mM, ethanol solution). Then, 20 μL of H2O2 with various concentrations was added into the mixture. The mixed solution was incubated at 40°C for 30 min. For comparison, the control experiments were also conducted under the same conditions by using bare RGO, INs or their physical mixture as catalysts. In addition, the influences of reaction buffer pH and incubation temperature on the peroxidase-like catalytic activity of the RGO-INs were also investigated.
With dihydrogen peroxide In aq. phosphate buffer at 35℃;
With Eu2O2S; dihydrogen peroxide In water; dimethyl sulfoxide at 40℃;
With dihydrogen peroxide; fluorescein In aq. acetate buffer at 35℃; for 0.5h;
With dihydrogen peroxide In aq. acetate buffer at 45℃; for 0.166667h;
With dihydrogen peroxide
With fluorescein In water for 0.166667h; Irradiation;
With 2-nitrobenzaldehyde/nanoceria hybrid(2-NBA/CeO2) In aq. buffer UV-irradiation;
With C32H34Cl3Co2N4O4S2; dihydrogen peroxide In ethanol Inert atmosphere;
With 2-amino-10-methyl-9-acridanone Irradiation;
With cholesterol oxidase In aq. buffer
With alpha-D-glucopyranose; dihydrogen peroxide In aq. acetate buffer at 50℃; for 0.166667h;
With dihydrogen peroxide In acetonitrile at 25℃; 2.5 Catalytic Activity Evaluation The POD activity was determined by adding 100 μL of TMB solution (10mM in acetonitrile) and 20μL of H2O2 solution(10mM) into 780μL of HAc/NaAc buffer (50mM, pH 4.0).Then, 100 μL of catalyst (1mg/mL) was added to the substrate solution to launch the reaction. The reaction was keptat 25°C, and the production of oxidized TMB (oxTMB) was quantified by monitoring the absorbance at 650nm using a UV-vis spectrophotometer (TU-1900, BPGI, China). The initial reaction rate (V0, μMmin-1) was calculated to represent the catalytic activity of the catalyst according to Eq.(1).where V0 is the initial catalytic rate (μM·min-1), OD650nm isthe absorbance at 650nm, a (39,000M-1·cm-1) is the molar extinction coefficient of oxTMB, b is the length of the optical path (cm), t is the reaction time (min).
With dihydrogen peroxide; (Fe3O)2(13+)*4H2O*CHO2(1-)*2C42H24N3O18P3(6-) In ethanol at 45℃; for 0.166667h;
With dihydrogen peroxide In aq. phosphate buffer at 42℃;
With iron(II,III) oxide; dihydrogen peroxide
With dihydrogen peroxide In aq. acetate buffer at 45℃; Enzymatic reaction; Evaluation of the POD-like activity of PCAZIF-L The POD-like feature of the obtained PCAZIF-L was photometrically studied by applying TMB and H2O2 as substrates. Here were different reaction systems: (a) 10 μL TMB (20 mM) + 990 μL NaAc-HAc buffer (0.2 M, pH 4.0), (b) 10 μL H2O2 (50 mM) + 990 μL NaAc-HAc buffer (0.2 M, pH 4.0), (c) 10 μL H2O2 (50 mM) + 10 μL TMB (20 mM) + 980 μL NaAc-HAc buffer (0.2 M, pH 4.0), (d) 10 μL PCAZIF-L (5 mg/mL) + 10 μL TMB (20 mM) + 980 μL NaAc-HAc buffer (0.2 M, pH 4.0), (e) 10 μL PCAZIF-L (5 mg/mL) + 10 μL H2O2 (50 mM) + 980 μL NaAc-HAc buffer (0.2 M, pH 4.0), (f) 10 μL PCAZIF-L (5 mg/mL) + 10 μL H2O2 (50 mM) + 10 μL TMB (20 mM) + 970 μL NaAc-HAc buffer (0.2 M, pH 4.0). After incubation in a water bath at 45 for 30 min, the UV-vis absorption spectra of the different reaction solutions were measured
With cobalt(II) chloride hexahydrate; oxygen; potassium hexacyanoferrate(III) In aq. phosphate buffer at 30℃;
With vermiculite; dihydrogen peroxide In aq. phosphate buffer at 37℃; Irradiation; Peroxidase-mimic activity evaluation of NSs Peroxidase-mimic activity evaluation of NSs to degrade H2O2 as substrate was performed using TMB as the indicator [60,61]. In brief, TMB solution (100 μg mL-1) was mixed with H2O2 (0, 1, 2.5, 5, 10, and 30 mM). Then 200 μg mL-1 NSs were added and the reaction was performed under 37 C in 15 min for steady-state kinetic analysis. To this end, absorbance peak values were collected and plotted with H2O2 concentration. Furthermore, linear Lineweaver-Burk plotting was performed using ε = 39,000 M-1 cm-1 for oxTMB to determine the Km and Vmax.
With vermiculite; dihydrogen peroxide In aq. phosphate buffer at 37℃; Irradiation; Peroxidase-mimic activity evaluation of NSs Peroxidase-mimic activity evaluation of NSs to degrade H2O2 as substrate was performed using TMB as the indicator [60,61]. In brief, TMB solution (100 μg mL-1) was mixed with H2O2 (0, 1, 2.5, 5, 10, and 30 mM). Then 200 μg mL-1 NSs were added and the reaction was performed under 37 C in 15 min for steady-state kinetic analysis. To this end, absorbance peak values were collected and plotted with H2O2 concentration. Furthermore, linear Lineweaver-Burk plotting was performed using ε = 39,000 M-1 cm-1 for oxTMB to determine the Km and Vmax.
With dihydrogen peroxide; sodium acetate; acetic acid at 300℃;
With dihydrogen peroxide In ethanol; water
With dihydrogen peroxide; ascorbic acid In aq. phosphate buffer
With Mn-based oxidase nanozyme
With dihydrogen peroxide In aq. acetate buffer
With dihydrogen peroxide In aq. acetate buffer at 40℃; 2.4. Colorimetric determination of L-Cys and H2O2 The experimental method for detecting H2O2 is as follows: Thecatalyst (60 g/mL) and TMB (0.4 mM) are added to a NaAc-HAc buffer solution (pH =3.5), and then a certain amount of H2O2 is introduced into this system. Subsequently, the system is incubated at 40C for 5 minutes. After filtration through a microporous membrane, the UV- visible spectrum of the filtrate is recorded. The experimental method for L-Cys detection is similar to that of H2O2 detection, with the only difference being the additional inclusion of a certain amount of L-Cys.
With PAA-Cu2MI
With Ir(III) complex anchored on (Zr63- O)43-OH)4) and 5,5′-dicarboxy-2,2′-bipydine based metal organic framework for 0.166667h; Irradiation;
With dihydrogen peroxide at 50℃; for 1.5h;
With 10% Fe doped MnOx nanostructure In aq. buffer
With dihydrogen peroxide; (69)Os037(30)Ru063 at 20℃; for 0.166667h;
With dihydrogen peroxide; 4CH2N5(1-)*3Cu(2+)*3H2O*2F(1-) at 40℃; for 0.5h; Peroxidase-like activity was evaluated using a colorimetricassay with the following protocol: Cu-MOF (ground powder,100 μg mL1), TMB (1 mM), and H2O2 (0.2 mM) werecombined in a reaction buffer at pH 4.0 with a total volumeof 9 mL. The reaction mixture was then incubated at40C for 30 min. Following incubation, the absorbance ofthe reaction solution was measured at a wavelengthof 652 nm8 using a UV-spectrophotometer. Optimal conditionswere determined through a series of experimentaltests varying catalyst concentration, H2O2 concentration,and TMB concentration.
With dihydrogen peroxide at 25℃; Irradiation;

References: [1]Nie, Guangdi; Zhang, Liang; Lei, Junyu; Yang, Liu; Zhang, Zhen; Lu, Xiaofeng; Wang, Ce [Journal of Materials Chemistry A, 2014, vol. 2, # 9, p. 2910 - 2914].
[2]Li, Lili; Zeng, Chunmei; Ai, Lunhong; Jiang, Jing [Journal of Alloys and Compounds, 2015, vol. 639, p. 470 - 477].
[3]Wang, Nan; Li, Bingchen; Qiao, Fengmin; Sun, Jianchao; Fan, Hai; Ai, Shiyun [Journal of Materials Chemistry B, 2015, vol. 3, # 39, p. 7718 - 7723].
[4]Ghosh, Abhisek Brata; Saha, Namrata; Sarkar, Arpita; Dutta, Amit Kumar; Biswas, Papu; Nag, Kamalaksha; Adhikary, Bibhutosh [New Journal of Chemistry, 2016, vol. 40, # 2, p. 1595 - 1604].
[5]Liu, Li; Shi, Ying; Yang, Yufang; Li, Menglu; Long, Yijuan; Huang, Yuming; Zheng, Huzhi [Chemical Communications, 2016, vol. 52, # 96, p. 13912 - 13915].
[6]Guo, Yali; Liu, Xiaoyu; Yang, Chengduan; Wang, Xudong; Wang, Dan; Iqbal, Anam; Liu, Weisheng; Qin, Wenwu [ChemCatChem, 2015, vol. 7, # 16, p. 2467 - 2474].
[7]Salarizadeh, Navvabeh; Sadri, Minoo; Sajedi, Reza H. [Applied Organometallic Chemistry, 2018, vol. 32, # 2].
[8]Liu, Li; Sun, Chaoqun; Yang, Juan; Shi, Ying; Long, Yijuan; Zheng, Huzhi [Chemistry - A European Journal, 2018, vol. 24, # 23, p. 6148 - 6154].
[9]Wang, Xiaopei; Gong, Ao; Luo, Wenhao; Wang, Haiqing; Lin, Changxu; Liu, Xiang Yang; Lin, Youhui [Chemical Communications, 2018, vol. 54, # 62, p. 8641 - 8644].
[10]Fontana, Liniquer André; Siqueira, Josiéli Demetrio; Ceolin, Joice; Iglesias, Bernardo Almeida; Piquini, Paulo Cesar; Neves, Ademir; Back, Davi Fernando [Applied Organometallic Chemistry, 2019, vol. 33, # 7].
[11]Xia, Yaokun; He, Wenhui; Li, Juan; Zeng, Lupeng; Chen, Tingting; Liao, Yijuan; Sun, Weiming; Lan, Jianming; Zhuo, Shuangmu; Zhang, Jing; Yang, Huanghao; Chen, Jinghua [Analytical Chemistry, 2019, vol. 91, # 13, p. 8406 - 8414].
[12]Gao, Jie; Wang, Caihong; Wang, Jinhong; Tan, Hongliang [Chemistry - A European Journal, 2019, vol. 25, # 41, p. 9629 - 9633].
[13]Ma, Chong-Bo; Zhang, Yu; Liu, Qiong; Du, Yan; Wang, Erkang [Analytical Chemistry, 2020, vol. 92, # 7, p. 5319 - 5328].
[14]Gao, Pengfei; Feng, Yaoyao; Wang, Mengfan; Jiang, Nan; Qi, Wei; Su, Rongxin; He, Zhimin [Catalysis Letters, 2021, vol. 151, # 2, p. 478 - 486].
[15]Wang, Xiao-Ning; Zhao, Yumeng; Li, Jia-Luo; Pang, Jian-Dong; Wang, Qiang; Li, Bao; Zhou, Hong-Cai [Dalton Transactions, 2021, vol. 50, # 11, p. 3854 - 3861].
[16]Chang, Mengyu; Hou, Zhiyao; Wang, Man; Yang, Chunzheng; Wang, Ruifeng; Li, Fang; Liu, Donglian; Peng, Tieli; Li, Chunxia; Lin, Jun [Angewandte Chemie - International Edition, 2021, vol. 60, # 23, p. 12971 - 12979][Angew. Chem., 2021, vol. 133, # 23, p. 13081 - 13089].
[17]Xu, Bolong; Li, Shanshan; Zheng, Lirong; Liu, Yunhang; Han, Along; Zhang, Jin; Huang, Zhijun; Xie, Haijiao; Fan, Kelong; Gao, Lizeng; Liu, Huiyu [Advanced Materials, 2022, vol. 34, # 15].
[18]He, Jiaqi; Zhuo, Tingling; Teng, Yintong; Chen, Guoqin; Zhao, Peng; Ou, Caiwen [Chinese Chemical Letters, 2023, vol. 34, # 2].
[19]Peng, Li-Jing; Yin, Shi-Jun; Chen, Li; Tian, Tao; Zhang, Wei-Yi; Zhou, Hang-Yu; Yang, Feng-Qing [New Journal of Chemistry, 2022, vol. 47, # 3, p. 1156 - 1164].
[20]Yu, Xinghua; Zhang, Ying-Chuan; Yang, Xing; Huang, Ziyang; Zhang, Tianfu; Yang, Liusi; Meng, Wenjing; Liu, Xiaotong; Gong, Ping; Forni, Alessandra; Zheng, Zheng; Liu, Bilu; Zhang, Pengfei; Cai, Lintao; Tang, Ben Zhong [Nano Today, 2022, vol. 44].
[21]Yu, Xinghua; Zhang, Ying-Chuan; Yang, Xing; Huang, Ziyang; Zhang, Tianfu; Yang, Liusi; Meng, Wenjing; Liu, Xiaotong; Gong, Ping; Forni, Alessandra; Zheng, Zheng; Liu, Bilu; Zhang, Pengfei; Cai, Lintao; Tang, Ben Zhong [Nano Today, 2022, vol. 44].
[22]Yuan, Minjia; Li, Qian; Wu, Zihe; Zhu, Huang; Gao, Yang; Zhou, Mi; Luo, Xianglin; Wang, Mao; Cheng, Chong [Small, 2023, vol. 19, # 52].
[23]Liu, Ying; Zhou, Xilong; Zhu, Weiran; Chen, Chen; Fan, Cunhao; Ding, Lijun; Wang, Kun [Inorganic Chemistry, 2023, vol. 62, # 37, p. 15022 - 15030].
[24]Badshah, Amir; Noreen, Sadaf; Shah, Mohibullah; Asad, Muhammad; Ullah, Riaz; Ali, Essam A.; Iqbal, Jibran; Sun, Wei; Nishan, Umar [RSC Advances, 2024, vol. 14, # 27, p. 19539 - 19549].
[25]Fatrekar, Adarsh P.; Morajkar, Rasmi V.; Vernekar, Amit A. [Chemical Science, 2024, vol. 15, # 36, p. 14726 - 14738].
[26]Geng, Xin; Song, Kexu; Hu, Qingying; Yin, Yue; Li, Haisong; Yan, Xiyun; Jiang, Bing [Journal of Materials Chemistry B, 2024, vol. 12, # 35, p. 8647 - 8654].
[27]Liang, Qiao-Ming; Liu, Jian; Liu, Yue-Xiao; Zhang, Hai-Yan; Xiao, Shi-Quan; Wang, Hai-Jun; Bu, Ming; Sun, Jing-Wen [Journal of Molecular Structure, 2025, vol. 1320].
[28]Gao, Ziyi; Cheng, Yujun; Long, Chuan; Tang, Wanli; Liu, Qi; Chen, Xiaoqing [Analytical Chemistry, 2025, vol. 97, # 11, p. 6136 - 6144].
[29]Zhou, Wenshuai; Qin, Haoran; Zhang, Qian; Cai, Jiaqi; Qi, Hetong; Qi, Honglan [Analytical Chemistry, 2025, vol. 97, # 12, p. 6555 - 6562].
[30]Yang, Yi; Sun, Huipeng; Han, Taihe; Hao, Qilong; Shen, Haofei; Jing, Yuanxue; Liu, Xiaoyan; Mu, Shuai; Zhang, Haixia [Analytical Chemistry, 2025, vol. 97, # 19, p. 10474 - 10483].
[31]Duhan, Udisha; Pan, Arnab; Dubey, Ritesh; Layek, Samar; Kumar, Sushil; Goswami, Tapas [Dalton Transactions, 2025, vol. 54, # 25, p. 9992 - 10002].
[32]Zhong, Yingying; Yang, Junsong; Liang, Qian; Yu, Xiao; Lai, Hongyu; Lin, Yu; Mo, Qijie; Wang, Qing; Chen, Zijian; Wang, Hongwu [RSC Advances, 2025, vol. 15, # 37, p. 30446 - 30455].
[33]Kim, Viktoriya; Lee, Dong Woo; Noh, Hye Ran; Kim, Jong-Yun; Pham, Tung Cao-Thanh; Lim, Sang Ho; Kim, Hyun Sung [Bulletin of the Korean Chemical Society, 2025, vol. 46, # 10, p. 957 - 967].
[34]Zhao, Qilin; Luan, Tian; Sun, Jian; Huang, Jianshe; Du, Yan; Yang, Xiurong [Analytical Chemistry, 2025, vol. 97, # 35, p. 19038 - 19046].
  • 17
  • [ 54827-17-7 ]
  • [ 2046947-10-6 ]
YieldReaction ConditionsOperation in experiment
With sulfuric acid; horseradishperoxidase; dihydrogen peroxide Enzymatic reaction;
With oxygen; phloxine B UV-irradiation;
With dihydrogen peroxide; sodium acetate; acetic acid In ethanol at 40℃; for 0.333333h;
With K(1+)*O40PW12(3-)*Fe(2+); dihydrogen peroxide In aq. acetate buffer at 20℃; for 0.0333333h;
With dihydrogen peroxide at 50℃;
With tert.-butylhydroperoxide; 3K(1+)*BO40W12(5-)*14H2O*4C3H3N4O2(1-)*2C3H4N4O2*3Cu(2+)
With dihydrogen peroxide; 3Fe(3+)*O(2-)*3HO(1-)*6Cu(2+)*2O(2-)*12H2O*6C4H2N2O2(2-) In aq. phosphate buffer at 37℃; for 0.166667h;
With manganese(IV) oxide In aq. acetate buffer at 37℃; Detection of RA First, oxTMB was prepared by adding 100 µL of TMB (10 mmol/L) and 10 µL of MnO2 nanosheets (4 mg/L) to 2.89 mL of HAc-NaAc buffer solution (0.2 mol/L, pH 5). After the above mixture liquid was held at 37 °C for 10 min, the MnO2 nanosheets were filtered to obtain blue oxTMB. Next, 1.5 mL of oxTMB and 500 µL of Y:Yb/Er-Cit-CD were mixed into a 4 mL centrifuge tube and incubated at 30 °C for 30 min. Further, different amounts of RA were added to the aforementioned solution, adjusting pH to 7 and mixed adequately. Then, the obtained mixture was incubated for 80 min at 40 °C. Lastly, the corresponding fluorescence emission spectra, UV-vis absorption spectra and temperature change of 808 nm laser irradiation (1.8 W/cm2) for 5 min were recorded.
With dihydrogen peroxide
With cerium-based metal-organic framework nanozyme In water at 30℃; for 0.416667h; 2.3.Dual-enzyme mimetic activities of UIO-66-NH2(Ce) The UIO-66-NH2(Ce) nanozyme solution was prepared by dissolving 6 mg of ground powder in 10 mL deionized water under 10 min ultrasonication, yielding a 0.6 mg•mL1 homogeneous suspension. The oxidase-like activity was evaluated using TMB as a chromogenicstrate. A reaction mixture containing 10 μL UIO-66-NH2(Ce) suspension (0.6 mg•mL1), 10 μL TMB (2 mM), 10 μL HAc-NaAc buffer (0.2 M, pH 4.0), and 70 μL deionized water was incubated at 30 C for 25 min. The UV-Vis spectrum was recorded between 500 and 750 nm.

  • 18
  • [ 54827-17-7 ]
  • [ 142827-59-6 ]
YieldReaction ConditionsOperation in experiment
89% With tert.-butylnitrite; trimethylsilylazide In acetonitrile at 0 - 25℃; for 3.5h; Inert atmosphere;
With tert.-butylnitrite; trimethylsilylazide In acetonitrile at -20 - 20℃;
  • 19
  • [ 54827-17-7 ]
  • [ CAS Unavailable ]
YieldReaction ConditionsOperation in experiment
With cadmium sulfate octahydrate; 1-thioglucose; oxygen; sodium salt of phosphorous acid; glucose oxidase In aq. buffer at 37℃; for 0.166667h; UV-irradiation; The Colorimetric Detection of Glucose Oxidase Activity A certain amount of 1-thio--D-glucose were incubated with different amounts of GOx in citratebuffer (10 mM, pH 4.0) for 60 min at 37 C. After that, 20 L Cd2+/Na3PO4 mixture was added to thesamples. Subsequently, the mixed solution was added with 20 L of 5 mM TMB and then diluted to200 L by acetate buffer (200 mM, pH 4.0) and illuminated under visible light irradiation ( ¥ 400 nm)for 10 min to allow development of the blue color, and the absorbance of the oxidized TMB (oxTMB) at652 nm was measured
With dihydrogen peroxide; sodium dodecyl-sulfate; 1-ethyl-3-methylimidazolium tetrafluoroborate; horseradish peroxidase In aq. phosphate buffer at 24.8 - 25.2℃; Enzymatic reaction; 4.4. Bioassay General procedure: The following experiments were measured under the sameconditions of 250.2 C and pH = 7.4. UV-vis spectra wereperformed by monitoring the absorbance at a specific time whenthe reaction occurs 5 min. Time-dependent curves were carried outin time course mode by monitoring the absorbance change at652 nm. The concentration of HRP, TMB and H2O2 is 0.05 mg/mL,200 mmol/L and 2 mmol/L respectively for all catalytic experimentunless otherwise stated. Firstly, 0.05 mg/mL HRP solutionsincubated for 1 h in different reaction mediums (pH = 7.4,250.2 C), then added 8 mL TMB (0.2 mol/L) stock solutionprepared in DMSO-d6 and 2 mL H2O2 (8.0 mmol/L) into theincubation mediums to obtain 8 mL reaction solutions, took3 mL sample for absorbance measurements.The kinetic assays of HRP-TMB-H2O2 were further conducted inthe mediums containing SDS (2.5 mmol/L) and SDS/[Emim][BF4](2.5 mmol/L/2.5 mmol/L) combination, kinetic parameters wereobtained by the enzyme kinetics theory and methods. The typicalMichaelis-Menten curve was obtained byfitting the Mentenequation. Kinetic assays were carried onfixing HRP concentrationat 0.05 mg/mL and varying concentrations of H2O2 and TMB.The activity assay of HRP was realized as follows. Firstly, 24 mLTMB stock solution (0.2 mol/L), 2 mL H2O2 (8.0 mol/L) were addedinto the mediums containing SDS/[Emim][BF4] (2.5 mmol/L/2.5 mmol/L) combinations, then added HRP solutions into the mixtures to obtain reaction solutions (8 mL) with different massconcentrations of HRP (10-50 mg/mL)and mixed quickly. Finally,took 3 mL sample for recording the absorbance at 652 nm within1 min.H2O2 detection was measured by the following steps. Firstly,8 mL TMB stock solution (0.2 mol/L), different volumes of H2O2were added into the mediums containing SDS/[Emim][BF4](2.5 mmol/L/2.5 mmol/L) combinations to obtain mixtures withdifferent H2O2 concentration (1 mmol/L-100 mmol/L). Then added4 mL HRP solution (0.1 mg/mL) into the mixtures to obtain reactionsolutions (8 mL) and mixed quickly. Finally, took 3 mL sample forrecording the absorbance at 652 nm at the specific time when thereaction occurred 3 min.
With GLUTATHIONE; copper peroxide for 1.5h; Acidic conditions;
 

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