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Chemical Structure| 162401-16-3 Chemical Structure| 162401-16-3

Structure of 162401-16-3

Chemical Structure| 162401-16-3

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Product Citations

Product Citations

Kofoed, Christian ; Erkalo, Girum ; Tay, Nicholas ES ; Ye, Xuanjia ; Lin, Yutong ; Muir, Tom W

Abstract: The surface landscapes of cells difer as a function of cell type and are frequently altered in disease contexts1–3. Exploiting such diferences is key to many therapeutic strategies and is the basis for developing diagnostic and basic-science tools. State-of-the-art strategies typically target single surface antigens, but each individual receptor rarely defnes the specifc cell type4,5. The development of programmable molecular systems that integrate multiple cell-surface features to convert on-target inputs to user-defned outputs is therefore highly desirable. Here we describe an autonomous decision-making device driven by proximity-gated protein trans-splicing that allows local generation of an active protein from two otherwise inactive polypeptide fragments. We show that this protein-actuator platform can perform convergent protein ligation on designated cell surfaces, allowing highly selective generation of active proteins, which can either remain physically associated with the cell surface on which they were manufactured or be released into the surrounding milieu. Because of its intrinsic modularity and tunability, we demonstrate that the technology is compatible with diferent types of input, targeting modality and functional output, allowing for the localized interrogation or manipulation of cellular systems.

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Product Details of [ 162401-16-3 ]

CAS No. :162401-16-3
Formula : C10H7N7O
M.W : 241.21
SMILES Code : NC1=NC(NN=C2)=C2C3=NC(C4=CC=CO4)=NN13
MDL No. :MFCD18914402
InChI Key :YKWJNVFPKLFRRE-UHFFFAOYSA-N
Pubchem ID :135485985

Safety of [ 162401-16-3 ]

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Application In Synthesis of [ 162401-16-3 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Downstream synthetic route of [ 162401-16-3 ]

[ 162401-16-3 ] Synthesis Path-Downstream   1~1

  • 1
  • [ 1200828-74-5 ]
  • [ 162401-16-3 ]
  • ethyl 2-(5-amino-2-(furan-2-yl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-7-yl)-2-cyclopropylacetate [ No CAS ]
  • ethyl 2-(5-amino-2-(furan-2-yl)-8H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-8-yl)-2-cyclopropylacetate [ No CAS ]
YieldReaction ConditionsOperation in experiment
51.4%; 64.7% With potassium carbonate; In N,N-dimethyl-formamide; at 60℃; To a stirred solution of 2- (furan-2-yl) -7H-pyrazolo [4, 3-e] [1, 2, 4] triazolo [1, 5-c] pyrimidin-5-amine (1.0 g, 4.2 mmol) in DMF (50 mL) was added K2CO3(1.3 g, 9.4 mmol) and <strong>[1200828-74-5]ethyl 2-bromo-2-cyclopropylacetate</strong> (1.2 g, 5.8 mmol) . After the addition, the reaction mixture was stirred overnight at 60 C. The reaction mixture was poured into H2O (50 mL) and extracted with EtOAc (100 mL x 3) . The combined organic layers were washed with brine, dried over Na2SO4, concentrated and purified by column chromatography (petroleum ether/EtOAc = 2: 1 1: 2) to give ethyl 2- (5-amino-2- (furan-2-yl) -7H-pyrazolo [4, 3-e] [1, 2, 4] triazolo [1, 5-c] pyrimidin-7-yl) -2-cyclopropylacetate (400 mg, 51.4%) and ethyl 2- (5-amino-2- (furan-2-yl) -8H-pyrazolo [4, 3-e] [1, 2, 4] triazolo [1, 5-c] pyrimidin-8-yl) -2-cyclopropylacetate (500 mg, 64.7%) as white solids. MS: M/e 368 (M+1)+.
 

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