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Chemical Structure| 3934-89-2 Chemical Structure| 3934-89-2

Structure of 3934-89-2

Chemical Structure| 3934-89-2

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Product Details of [ 3934-89-2 ]

CAS No. :3934-89-2
Formula : C9H10O4
M.W : 182.18
SMILES Code : CC(C1=CC(OC)=C(O)C(O)=C1)=O

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Application In Synthesis of [ 3934-89-2 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Downstream synthetic route of [ 3934-89-2 ]

[ 3934-89-2 ] Synthesis Path-Downstream   1~2

  • 1
  • [ 3934-89-2 ]
  • S-adenosyl-L-methionine [ No CAS ]
  • [ 2478-38-8 ]
YieldReaction ConditionsOperation in experiment
With crude enzymatic extracts from TX1 tobacco (Nicotiana tabacum L. cv. Xanthi) cells collected 72 h after MeJa treatment; In aq. buffer; at 30℃; for 0.5h;pH 8.5;Enzymatic reaction;Kinetics; 5-HAV-OMT activity was measured by incubating a mixture containing 150mul protein extract, 335mul 0.1M Tris-HCl pH 8.5, 5mul 20mM SAM and 10mul 5-hydroxyacetovanillone 10mM at 30C in an Eppendorf tube. After 30min, the reaction was stopped with 50mul acetic acid. After precipitation of the proteins at 4C and centrifugation, a 10mul aliquot was analysed by HPLC and the amount of acetosyringone formed during the reaction was quantified. When more than 30% of the substrate was consumed, a shorter reaction time was used. This assay was used to determine the optimum pH, using KPi, Tris-HCl and NaHCO3-Na2CO3 buffers. The effect of cations on the activity was tested by adding MgCl2 or CaCl2 (1mM final concentration) to the incubation medium. Inhibition of 5-HAV-OMT activity by anti-COMT antibodies was tested by mixing protein extracts with 10% (v/v) serum at room temperature for 10min before assaying residual activity as described above.
  • 2
  • [ 2478-38-8 ]
  • [ 3934-89-2 ]
  • [ 33709-29-4 ]
 

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