Home Cart Sign in  
Chemical Structure| 60-00-4 Chemical Structure| 60-00-4
Chemical Structure| 60-00-4

*Storage: {[sel_prStorage]}

*Shipping: {[sel_prShipping]}

,{[proInfo.pro_purity]}

EDTA is used as a hexadentate ligand and chelating agent.

Synonyms: Ethylenediaminetetraacetic acid

4.5 *For Research Use Only !

{[proInfo.pro_purity]}
Cat. No.: {[proInfo.prAm]} Purity: {[proInfo.pro_purity]}

Change View

Size Price VIP Price

US Stock

Global Stock

In Stock
{[ item.pr_size ]} Inquiry {[ getRatePrice(item.pr_usd,item.pr_rate,item.mem_rate,item.pr_is_large_size_no_price, item.vip_usd) ]}

US Stock: ship in 0-1 business day
Global Stock: ship in 5-7 days

  • {[ item.pr_size ]}

In Stock

- +

Please Login or Create an Account to: See VIP prices and availability

US Stock: ship in 0-1 business day
Global Stock: ship in 2 weeks

  • 1-2 Day Shipping
  • High Quality
  • Technical Support
Product Citations

Product Citations      Show More

Lawson, Harrison Douglas ; Nguyen, Huy Hoang ; Lee, Keng‐Jung ; Wongsuwan, Nattarat ; Tupe, Ayesha ; Lu, Mengrou , et al.

Abstract: Metal-organic frameworks (MOFs) have evolved from uses in catalysis and gas storage to exciting applications in biomedicine, particularly in drug delivery. Initially, MOFs are primarily used to deliver small molecules, recent innovations have shifted focus toward more complex nucleic acids like DNA, short guide RNA (sgRNA), and short interfering RNA (siRNA). Remarkably, no studies to date have demonstrated the encapsulation and delivery of messenger RNA (mRNA) via MOFs in vitro and in vivo. This study addresses that gap by identifying synthetic conditions to encapsulate and deliver mRNA using zeolitic imidazole framework-8 (ZIF-8). Early attempts show mRNA loading in ZIF-8 but loss of mRNA in biological media. To overcome this challenge, polyethyleneimine (PEI) is incorporated into the formulation, forming a robust polymer complex core-MOF shell particle. This system stabilizes mRNA complexes and delays their release, resulting in effective protein expression in multiple cell lines and mice, performing on par with commercial lipid-based systems. Here, the first investigation into thermally stable mRNA storage using ZIF-8 demonstrates successful protein expression after three months of room-temperature storage in vitro and one month in vivo. These findings broaden the scope of MOF-based therapeutic delivery and open new avenues for long-term mRNA storage and transport.

Keywords: drug delivery ; in vivo ; metal-organic frameworks ; mRNA ; nucleic acid ; stability

Purchased from AmBeed:

Kaustav Khatua ; Yugendar R. Alugubelli ; Kai S. Yang ; Veerabhadra R. Vulupala ; Lauren R. Blankenship ; Demonta Coleman , et al.

Abstract: The main (MPro) of SARS-CoV-2, the causative agent of COVID-19, is a pivotal nonstructural critical for viral replication and pathogenesis. Its function relies on three active site pockets for substrate recognition and a catalytic for enzymatic activity. To develop potential SARS-CoV-2 , we successfully synthesized a diverse range of azapeptide inhibitors with various covalent warheads to target MPro's catalytic . Our characterization identified potent MPro inhibitors, including MPI89 that features an aza-2,2-dichloroacetyl warhead with a remarkable EC50 value of 10 nM against SARS-CoV-2 infection in ACE2+ A549 cells and a selective index of 875. MPI89 is also remarkably selective and shows no potency against SARS-CoV-2 papain-like and several human proteases. Crystallography analyses demonstrated that these inhibitors covalently engaged the catalytic and used the aza-amide carbonyl oxygen to bind to the oxyanion hole. MPI89 stands as one of the most potent MPro inhibitors, suggesting the potential for further exploration of azapeptides and the aza-2,2-dichloroacetyl warhead for developing effective therapeutics against COVID-19.

Keywords: COVID-19 ; SARS-CoV-2 ; Main protease ; Azapeptide ; Covalent inhibitor

Purchased from AmBeed: ; ; ;

Harrison D. Lawson ; Huy H. Nguyen ; Ayesha Y. Tupe ; Mariah L. Arral ; Keng J.Lee ; Mengrou Lu , et al.

Abstract: Over the last two decades, the utility of metal-organic frameworks (MOFs) has expanded from catalysis and gas storage to biomedical applications such as drug delivery. At first, MOF-based delivery platforms focused on delivering small molecules, with current work focusing on nucleic acids, such as DNA, short guide RNA (sgRNA), and short interfering RNA (siRNA). No studies to this date have specifically shown the encapsulation and delivery of mRNA with MOFs, possibly due to the fragile nature of messenger RNA (mRNA). This study explores and identifies suitable synthetic conditions for encapsulating and delivering mRNA with zeolitic imidazole framework-8 (ZIF-8). Initial ZIF-8 encapsulation attempts, although capable of mRNA loading, could not retain mRNA longer than 1 hour in biological media. To address this issue, we added polyethyleneimine (PEI) to the matrix, enabling the retention of mRNA with 4 hours of stability. Polyethyleneimine incorporation resolves the leakage of mRNA from ZIF-8, enabling delivery and resultant protein expression in multiple cell lines comparable to commercial lipid transfection reagents. Furthermore, we report the first application exploring thermally stable mRNA storage with ZIF-8 with successful protein expression achieved after 3 months of room temperature storage. Together, this work expands the catalog of therapeutics MOFs can deliver.

Keywords: Metal-Organic Framework ; Biomolecules ; Drug Delivery ; mRNA ; Nucleic Acid ; Stability

Purchased from AmBeed:

Alternative Products

Product Details of EDTA

CAS No. :60-00-4
Formula : C10H16N2O8
M.W : 292.24
SMILES Code : OC(CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O)=O
Synonyms :
Ethylenediaminetetraacetic acid
MDL No. :MFCD00003541
InChI Key :KCXVZYZYPLLWCC-UHFFFAOYSA-N
Pubchem ID :6049

Safety of EDTA

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H319-H413
Precautionary Statements:P273-P301+P312+P330-P305+P351+P338

Application In Synthesis of EDTA

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Upstream synthesis route of [ 60-00-4 ]

[ 60-00-4 ] Synthesis Path-Upstream   1~1

  • 1
  • [ 60-00-4 ]
  • [ 50-00-0 ]
  • [ 24123-14-6 ]
  • [ 124-38-9 ]
References: [1] Acta Chimica Hungarica, 1987, vol. 124, # 4, p. 557 - 566.
 

Historical Records

Technical Information

Categories