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CAS No. : | 624-75-9 | MDL No. : | MFCD00001886 |
Formula : | C2H2IN | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | VODKOOOHHCAWFR-UHFFFAOYSA-N |
M.W : | 166.95 | Pubchem ID : | 69356 |
Synonyms : |
|
Num. heavy atoms : | 4 |
Num. arom. heavy atoms : | 0 |
Fraction Csp3 : | 0.5 |
Num. rotatable bonds : | 0 |
Num. H-bond acceptors : | 1.0 |
Num. H-bond donors : | 0.0 |
Molar Refractivity : | 24.44 |
TPSA : | 23.79 Ų |
GI absorption : | High |
BBB permeant : | Yes |
P-gp substrate : | No |
CYP1A2 inhibitor : | No |
CYP2C19 inhibitor : | No |
CYP2C9 inhibitor : | No |
CYP2D6 inhibitor : | No |
CYP3A4 inhibitor : | No |
Log Kp (skin permeation) : | -6.85 cm/s |
Log Po/w (iLOGP) : | 0.99 |
Log Po/w (XLOGP3) : | 0.66 |
Log Po/w (WLOGP) : | 0.94 |
Log Po/w (MLOGP) : | 0.6 |
Log Po/w (SILICOS-IT) : | 0.94 |
Consensus Log Po/w : | 0.83 |
Lipinski : | 0.0 |
Ghose : | None |
Veber : | 0.0 |
Egan : | 0.0 |
Muegge : | 3.0 |
Bioavailability Score : | 0.55 |
Log S (ESOL) : | -1.29 |
Solubility : | 8.54 mg/ml ; 0.0512 mol/l |
Class : | Very soluble |
Log S (Ali) : | -0.74 |
Solubility : | 30.7 mg/ml ; 0.184 mol/l |
Class : | Very soluble |
Log S (SILICOS-IT) : | -1.31 |
Solubility : | 8.12 mg/ml ; 0.0486 mol/l |
Class : | Soluble |
PAINS : | 0.0 alert |
Brenk : | 2.0 alert |
Leadlikeness : | 1.0 |
Synthetic accessibility : | 4.11 |
Signal Word: | Danger | Class: | 8,6.1 |
Precautionary Statements: | P210-P264-P270-P280-P301+P310+P330-P301+P330+P331-P303+P361+P353-P304+P340+P310-P305+P351+P338+P310-P363-P370+P378-P403+P235-P405-P501 | UN#: | 2922 |
Hazard Statements: | H227-H301-H314 | Packing Group: | Ⅱ |
GHS Pictogram: |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
100% | With sodium iodide In acetone | Step 2. The method follows that of S5; 2, 6-piperidinedimethanol (3.80 g, 26.21 mmol), iodoacetonitrile (5.24 g, 31.45 mmol), Et3N (4. 38 mL, 31.45 mmol) and dry DMF (20 mL). Purification by flash chromatography using CH2CI2/CH30H (9: 1) as eluent afforded the title compound as a straw-coloured oil (2.5 g, 53 percent). FAB MS, m/z (M+H) + 185. The iodoacetonitrile was prepared by the use of the Finkelstein reaction. BrCH2CN (3.77 g, 0.0314 mmol) was added dropwise to a stirred solution of Nal (4.71 g, 0.031 mmol) in acetone. Precipitation of NaBr occurred within a few minutes and indicated that exchange of the halides had taken place. Sodium bromide was filtered off, and the acetone was removed in vacuo. Crude yield (5.24 g, 100 percent). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
70% | In tetrahydrofuran; toluene; at 0 - 20℃; for 51h;Inert atmosphere; | A. A solution of trimethylphosphine in toluene (1 M, 60 mL, 60 mmol) at 0 C under nitrogen was diluted with toluene(30 mL) and tetrahydrofuran (30 mL). <strong>[624-75-9]Iodoacetonitrile</strong> (4.2 mL, 9.69 g, 58 mmol) was then added dropwise whilestirring vigorously, whereby a colorless solid precipitated. When the addition was finished, the ice-bath was removedand stirring was continued at ambient temperature for 51 h. The mixture was filtered, and the solid was washed withtoluene and dried under reduced pressure. Recrystallization from acetonitrile (37.5 mL) to give the compound ascolorless crystals (9.89 g, yield: 70%). |
68.3% | In tetrahydrofuran; toluene; at 20℃;Cooling with ice; | Cyanomethyl)trimethylphosphonium iodide was prepared according to the general method described in Zaragoza, F., et al., J. Org. Chem.2001, 66, 2518-2521. In a 1 L round bottom flask, trimethylphosphine in toluene (100 mL, 100 mmol) was diluted with THF (50.0 mL) and toluene (50.0 mL), and cooled on an ice bath. The reaction mixture was stirred vigorously while <strong>[624-75-9]iodoacetonitrile</strong> (7 mL, 16.7 g, 68.3 mmol) was added dropwise to produce a tan precipitate. The cooling bath was removed and the reaction mixture was stirred overnight at room temperature. The flask was placed in a sonicator to break up any clumped solids. The reaction mixture was stirred an additional 4 hours. The solids were collected by filtration and dried under vacuum to give (1380) (cyanomethyl)trimethylphosphonium iodide (16.6 g, 68.3 mmol, 68.3 % yield). 1H NMR (400 MHz, DMSO-d6) 4.03 (d, J=16.4 Hz, 2H), 2.05 (d, J=15.4 Hz, 9H). |
66% | In toluene; at 0 - 20℃; for 40h; | Cyanomethyl-trimethyl-phosphonium iodide (TJA01110); C5H11INP MW 243.03 Trimethylphosphine in THF (1M, 20.0 mL, 20.0 mmol) at 0 C under N2 (g) was diluted with anhydrous toluene (40 mL). <strong>[624-75-9]Iodoacetonitrile</strong> (1.40 mL, 19.4 mmol) was added dropwise with vigorous stirring forming a white ppt. The mixture was allowed to warm to r. t. and left to stir for 40 h. The mixture was filtered and washed with toluene to give a white solid which was dried under vacuum. Recrystallisation (acetonitrile) provided the title compound as a white crystalline solid (3.23 g, 66 %), ¹H NMR (300 MHz, DMSO-d6) No. 2.01-2.06 (9H, d, J= 15.3 Hz, P (CH3)3), (2H, d, J= 16.4 Hz, PCH2CN); 3¹p NMR (121.5 MHz, DMSO-d6) No. 32.9. |
66% | In toluene; at 0 - 20℃; for 40h; | Cyanomethyl-trimethyl-phosphonium iodide (TJA01110) C5H11INP MW 243.03. Trimethylphosphine in THF (IM, 20.0 mL, 20.0 mmol) at 0 0C under N2 (g) was diluted with anhydrous toluene (40 mL). <strong>[624-75-9]Iodoacetonitrile</strong> (1.40 mL, 19.4 mmol) was added dropwise with vigorous stirring forming a white ppt. The mixture was allowed to warm to r.t. and left to stir for 40 h. The mixture was filtered and washed with toluene to give a white solid which was dried under vacuum. Recrystallisation (acetonitrile) provided the title compound as a white crystalline solid (3.23 g, 66 %), 1H NMR (300 MHz, DMSO-J6) delta 2.01-2.06 (9H, d, J= 15.3 Hz, P(CHs)3), 4.01-4.07 (2H, d, J= 16.4 Hz, PCH2CN);31P NMR (121.5 MHz, DMSO-J6) delta 32.9. |
The synthesis of (cyanomethyl)trimethylphosphonium iodide is carried out in very much the same way as the procedure described in the literature [J. Org. Chem. 2001, 66, 2518-2521] from trimethylphosphine and <strong>[624-75-9]iodoacetonitrile</strong>. 2.42 g of (cyanomethyl)trimethylphosphonium iodide is added to a solution of 1 g of alcohol 8 in 18 ml of propionitrile, and then 2.1 ml of diisopropyl ethylamine is added in drops. The reaction mixture is stirred for 14 hours at 97 C. After cooling to room temperature, the batch is mixed with 10 ml of water and 1 ml of concentrated hydrochloric acid. The phase separation is carried out between ethyl acetate/water. The organic phase is washed with water and saturated sodium chloride solution, dried on magnesium sulfate and concentrated by evaporation. Column-chromatographic purification (cyclohexane/ethyl acetate) yields 480 mg of cyanide 11b as a colorless foam (GC-MS: m/z theor.: 535, pract.: 535). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
77% | With potassium carbonate; In acetonitrile; at 0 - 20℃; for 16h; | A 50 mL round-bottomed flask equipped with a magnetic stirrer was charged with 1-(2-fluorobenzenesulfonyl)-4-hydroxy-1H-indole (0.600 g, 0.002 mol), 25 mL of anhydrous acetonitrile and potassium carbonate (1.104 g, 0.008 mol). The reaction mixture was cooled to 0 C. and <strong>[624-75-9]iodoacetonitrile</strong> (0.500 g, 0.003 mol) was added dropwise over five minutes. The reaction mixture was allowed to warm to ambient temperature and stirred for eighteen hours. The mixture was taken in ether (200 mL), washed with water (2×30 mL) and brine (1×30 mL), dried over MgSO4 and concentrated in vacuo. The resulting oily brown residue was purified by flash chromatography (8:2 ethyl acetate/hexanes) to give [1-(2-fluoro-benzenesulfonyl)-1H-indol-4-yloxy]acetonitrile as a crystalline solid (0.507 g, 77%). 1H NMR (300 MHz, CDCl3) delta: 4.87 (s, 2H), 6.76 (d, 1H. J=13.18), 6.77 (d, 1H, J=1.51), 7.11 (dt, 1H, J=8.29, J=1.13), 7.23 (t, 1H, J=8.29), 7.29 (dt, 1H, J=7.73, J=1.13), 7.58 (m, 4H), 8.03 (m, 1H). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
52% | With potassium carbonate; In acetonitrile; at 20℃; for 408h; | Compound 6 (prepared according to B2. b) (30 mg) was dissolved in 20 ml of MECN, and iodo acetonitrile (1.0 eq. ) and K2CO3 (3 eq, 29 mg) were added. Stirring for 17 days at 20C. The MECN was evaporated and the residue was purified by preparative TLC using CHCL2/MEOH (9/1) as the eluent. Yield: compound 60 (52 %). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
38% | With caesium carbonate; In DMF (N,N-dimethyl-formamide); at 65℃; for 0.5h; | A mixture of [4- (4-HYDROXYPHENYL) butyl] carbamic acid tert-butyl ester 31 (0.365 g, 1.37 mmol) and Cs2CO3 (0.672 g, 2.06 mmol) in anhydrous DMF (8 mL) was heated at 65 C for 30 min. IODOACETONITRILE (0.276 g, 1.651 mmol) was then added to the mixture in one portion. The mixture was stirred at 65 C overnight, and then cooled to room temperature. The precipitated solid was filtered, and the filtrate was partitioned between water and dichloromethane (each 50 mL). The organic layer was separated, washed with brine (3 x 50 mL), dried over anhydrous NA2S04 and concentrated under vacuum. The residue was chromatographed on silica gel, eluting with a mixture of diethyl ether/dichloromethane (6: 94, v/v), to afford the desired product 40 (0.109 g, 38% yield) as a colorless viscous OIL. 1H NMR (300 MHz, CDC13) : 8 1.43 (s, 9H), 1.57 (M, 4H), 2.60 (t, 2H), 3.15 (M, 2H), 4.49 (br, 1H), 4.75 (s, 2H), 6.91 (d, 2H), 7.13 (d, 2H). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
63% | With potassium carbonate; In DMF (N,N-dimethyl-formamide); at 60℃; for 0.5h; | To a mixture of 5-bromo-2-isopropyl-4-methoxy-phenol from step 4 (0.831 g, 3.4 mmol) and K2CO3 (0.562 g, 4.1 mmol) in 17 mL dimethyl formamide (DMF) was added <strong>[624-75-9]iodoacetonitrile</strong> (0.594 g, 3.6 mmol). The mixture was warmed to 60 C. for 30 minutes and then allowed to cool to room temperature. After cooling to room temperature the mixture was taken up in 50 mL of H2O and extracted with 1:1 toluene/ethyl acetate, washed with H2O and then with brine. The combined organic layers were dried over Na2SO4, filtered and conectrated in vacuo to give a crude solid. Purification via flash chromatography (1:1 hexane/CH2Cl2) afforded (5-bromo-2-isopropyl-4-methoxy-phenoxy)-acetonitrile (0.611 g, 63%) as a while solid. |
63% | With potassium carbonate; In N,N-dimethyl-formamide; at 60℃; for 0.5h; | To a mixture of 5-bromo-2-isopropyl-4-methoxy-phenol from step 4 (0.831 g, 3.4 mmol) and K2CO3 (0.562 g, 4.1 mmol) in 17 mL dimethyl formamide (DMF) was added <strong>[624-75-9]iodoacetonitrile</strong> (0.594 g, 3.6 mmol). The mixture was warmed to 60 C. for 30 minutes and then allowed to cool to room temperature. After cooling to room temperature the mixture was taken up in 50 mL of H2O and extracted with 1:1 toluene/ethyl acetate, washed with H2O and then with brine. The combined organic layers were dried over Na2SO4, filtered and concentrated in vacuo to give a crude solid. Purification via flash chromatography (1:1 hexane/CH2Cl2) afforded (5-bromo-2-isopropyl-4-methoxy-phenoxy)-acetonitrile (0.611 g, 63%) as a while solid. |
63% | With potassium carbonate; In N,N-dimethyl-formamide; at 60℃; for 0.5h; | To a mixture of 5-bromo-2-isopropyl-4-methoxy- phenol from step 4(0.831 g, 34 mmol) and K2C03 (0.562 g, 4.1 mmol) in 17 mE dimethyl formamide (DMF) was added <strong>[624-75-9]iodoacetonitrile</strong> (0.594 g, 3.6 mmol). The mixture was warmed to 60 C. for 30 minutes and then allowed to cool to room temperature. After cooling to room temperature the mixture was taken up in SOmE of H20 and extracted with 1:1 toluene/ethyl acetate, washed with H20 and then with brine. The combined organic layers were dried over Na2504, filtered and concentrated in vacuo to give a crude solid. Purification via flash chromatography (1:1 hexane/CH2C12) afforded (5-bromo-2-isopropyl-4-methoxy-phenoxy)-aceto- nitrile (0.611 g, 63%) as a while solid. |
63% | With potassium carbonate; In N,N-dimethyl-formamide; at 60℃; for 0.5h; | j181j To a mixture of 5-bromo-2-isopropyl-4-methoxy-phenol from step 4 (0.831 g, 3.4 mmol) and K2C03 (0.562 g, 4.1 mmol) in 17 mL dimethyl formamide (DMF) was added <strong>[624-75-9]iodoacetonitrile</strong> (0.594 g, 3.6 mmol). The mixture was warmed to 60 C for 30 minutes and then allowed to cool to room temperature. After cooling to room temperature the mixture was taken up in 50 mL of H20 and extracted with 1:1 toluene/ethyl acetate, washed with H20 and then with brine. The combined organic layers were dried over Na2SO4, filtered and concentrated in vacuo to give a crude solid. Purification via flash chromatography (1:1 hexane/CH2C12) afforded (5-bromo-2-isopropyl-4-methoxy-phenoxy)-acetonitrile (0.611 g, 63%) as a while solid. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
In tetrahydrofuran; ethyl acetate; | e) To a solution of 7-benzyloxymethyl-8-(4-hydroxyphenyl)-1,3-dipropyl-1,3,7-trihydropurine-2,6-dione (1 g, 2.2 mmol) in tetrahydrofuran (20 ml) was added potassium t-butoxide (0.28 g, 2.4 mmol), and the mixture stirred for 30 minutes at room temperature. <strong>[624-75-9]Iodoacetonitrile</strong> (0.38 g, 2.23 mmol) was then added, and the mixture stirred for 16 hours at room temperature. The solvent was removed under reduced pressure, and the residue was dissolved in ethyl acetate and passed through a silica gel plug, to provide 7-benzyloxymethyl-8-(4-cyanomethoxyphenyl)-1,3-dipropyl-1,3,7-trihydropurine-2,6-dione, a compound of formula (7) | |
In tetrahydrofuran; ethyl acetate; | e) To a solution of 7-benzyloxymethyl-8-(4-hydroxyphenyl)-1,3-dipropyl-1,3,7-trihydropurine-2,6-dione (1 g, 2.2 mmol) in tetrahydrofuran (20 ml) was added potassium t-butoxide (0.28 g, 2.4 mmol), and the mixture stirred for 30 minutes at room temperature. <strong>[624-75-9]Iodoacetonitrile</strong> (0.38 g, 2.23 mmol) was then added, and the mixture stirred for 16 hours at room temperature. The solvent was removed under reduced pressure, and the residue was dissolved in ethyl acetate and passed through a silica gel plug, to provide 7-benzyloxymethyl-8-(4-cyanomethoxyphenyl)-1,3-dipropyl-1,3,7-trihydropurine-2,6-dione, a compound of formula (7) | |
e) To a solution of 7-benzyloxymethyl-8-(4-hydroxyphenyl)-1,3-dipropyl-1,3,7-trihydropurine-2,6-dione (1 g, 2.2 mmol) in tetrahydrofuran (20 ml) was added potassium t-butoxide (0.28 g, 2.4 mmol), and the mixture stirred for 30 minutes at room temperature.<strong>[624-75-9]iodoacetonitrile</strong> (0.38 g, 2.23 mmol) was then added, and the mixture stirred for 16 hours at room temperature.The solvent was removed under reduced pressure, and the residue was dissolved in ethyl acetate and passed through a silica gel plug, to provide 7-benzyloxymethyl-8-(4-cyanomethoxyphenyl)-1,3-dipropyl-1,3,7-trihydropurine-2,6-dione, a compound of formula (7) |
In tetrahydrofuran; ethyl acetate; | e) To a solution of 7-benzyloxymethyl-8-(4-hydroxyphenyl)-1,3-dipropyl-1,3,7-trihydropurine-2,6-dione (1 g, 2.2 mmol) in tetrahydrofuran (20 ml) was added potassium t-butoxide (0.28 g, 2.4 mmol), and the mixture stirred for 30 minutes at room temperature. <strong>[624-75-9]Iodoacetonitrile</strong> (0.38 g, 2.23 mmol) was then added, and the mixture stirred for 16 hours at room temperature. The solvent was removed under reduced pressure, and the residue was dissolved in ethyl acetate and passed through a silica gel plug, to provide 7-benzyloxymethyl-8-(4-cyanomethoxyphenyl)-1,3-dipropyl-1,3,7-trihydropurine-2,6-dione, a compound of formula (7) | |
To a solution of 7-BENZYLOXYMETHYL-8- (4-HYDROXYPHENYL)-1, 3-DIPROPYL-1, 3,7- trihydropurine-2,6-dione (LG, 2. 2MMOL) in tetrahydrofuran (20ML) was added potassium t- butoxide (0.28g, 2. 4MMOL), and the mixture stirred for 30 minutes at room temperature. IODOACETONITRILE (0. 38G, 2. 23MMOL) was then added, and the mixture stirred for 16 hours at room temperature. The solvent was removed under reduced pressure, and the residue was dissolved in ethyl acetate and passed through a silica gel plug, to provide 7-BENZYLOXYMETHYL-8- (4- cyanomethoxyphenyl)-1, 3-dipropyl-1, 3,7-trihydropurine-2, 6-dione, a compound of formula (7) |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With potassium hydroxide; acetic acid; In ethanol; water; | a (2-Amino-3,5-difluoro-phenylsulfanyl)acetonitrile A suspension of <strong>[119256-40-5]2-amino-4,6-difluoro-benzothiazole</strong> (7.00 g) was stirred for 6 h at 160 C. under nitrogen in a solution of potassium hydroxide (12.6 g) in water (20 ml). The resulting mixture was cooled to 0-5 C. and a solution of acetic acid (8.85 ml) in water (10 ml) and subsequently ethanol (40 ml) and iodoacetonitrile (2.75 ml) were added. After 30 min the mixture was neutralized with acetic acid (2.25 ml) and filtered. The filtrate was evaporated to approx. 30 ml and diluted with water (150 ml). A yellow precipitate was filtered off and dried. The crude product was purified by flash chromatography on silica gel eluted with ethyl acetate-heptane (3:7) yielding the title compound, Rf=0.26, as orange crystals, mp 66-67 C.; 1H-NMR (DMSO-d6), delta (ppm): 7.30-7.07 (m, 2H, Ar-H), 5.33 (br, 2H, NH2), 4.05 (s, 2H, CH2). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With potassium hydroxide; acetic acid; In ethanol; water; | a (2-Amino-4-fluoro-phenylsulfanyl)acetonitrile A suspension of <strong>[20358-07-0]2-amino-5-fluoro-benzothiazole</strong> (3.45 g) was stirred for 7 h at 165-170 C. under nitrogen in a solution of potassium hydroxide (6.9 g) in water (13.5 ml). The resulting mixture was cooled on an ice bath to 10 C., supplemented with water (5 ml) and then a solution of acetic acid (3.5 ml) in water (4 ml). The mixture was diluted with 96% ethanol (20 ml) and subsequently a solution of iodoacetonitrile (3.42 g) in 96% ethanol (5 ml) was added. After stirring for 15 min the mixture was adjusted to pH ~7 by adding acetic acid and approx. half of the solvent was removed in vacuo. The residue was diluted with water (100 ml) and extracted with ethyl acetate (50+3*25 ml). The combined extracts were dried over sodium sulfate, filtered and evaporated to give the title compound as a practically pure yellow oil. Yield 3.60 g (96%); 1H-NMR (DMSO-d6), delta (ppm): 7.32-7.43 (dd, 1H, Ar-H), 6.49-6.60 (dd, 1H, Ar-H), 6.29-6.43 (ddd, 1H, Ar-H), 5.88 (br, 2H, NH2), 3.82 (s, 2H, CH2). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
97% | To a solution of 5-chloro-2-isopropyl-4-methoxy-phenol (10.36 g, 51.6 mmol) in 40 mL DMF was added K2CO3 (8.55 g, 62.0 mmol) and the mixture was heated to 65 C. After 15 minutes <strong>[624-75-9]iodoacetonitrile</strong> (9.05 g, 54.2 mmol) was added and the mixture was heated to 80 C. for 1 hour. The mixture was cooled, poured into an ice/H2O mixture and extracted with 1:1 toluene/hexane. The combined organics were washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The crude product was purified by passing through a short plug of silica to afford (5-chloro-2-isopropyl-4-methoxy-phenoxy)-acetonitrile (11.97 g, 97%) as a white solid. | |
97% | To a solution of 5-chloro-2-isopropyl-4-methoxy-phenol (10.36 g, 51.6 mmol) in 40 mL DMF was added K2CO3 (8.55 g, 62.0 mmol) and the mixture was heated to 65 C. After 15 minutes <strong>[624-75-9]iodoacetonitrile</strong> (9.05 g, 54.2 mmol) was added and the mixture was heated to 80 C. for 1 hour. The mixture was cooled, poured into an ice/H2O mixture and extracted with 1:1 toluene/hexane. The combined organics were washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The crude product was purified by passing through a short plug of silica to afford (5-chloro-2-isopropyl-4-methoxy-phenoxy)-acetonitrile (11.97 g, 97%) as a white solid. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
74% | Example 346-{(SR)-1-[(3RS,4SR)-1-(1-Cyanomethyl-piperidine-4-carbonyl)-4-(3,4-dichloro-phenyl)-pyrrolidin-3-yl]-ethoxy}-nicotinonitrile; To a stirred solution of 6-{(SR)-1-[(3RS,4SR)-4-(3,4-dichloro-phenyl)-1-(piperidine-4-carbonyl)-pyrrolidin-3-yl]-ethoxy}-nicotinonitrile (VIII-B-1) (25 mg, 0.053 mmol) in THF (2 mL) was added NaH (2.4 mg, 55% purity, 0.056 mmol). After 10 min. 2-iodo acetonitrile (13 mg, 0.079 mmol) was added and stirring was continued at RT overnight.The reaction was quenched with H2O, and the product extracted with EtOAc. The combined organic phases were dried over Na2SO4, concentrated under vacuo and the residue was purified by column chromatography (SiO2, CH2Cl2/MeOH 9/1) to yield 20 mg (74%) of the title compound as a light brown foam. ES-MS m/e: 512.0 (M+H+). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
88% | EXAMPLE 163 4-Chloro-N-[(3RS,4SR)-4-(4-chloro-phenyl)-1-(1-cyanomethyl-piperidine-4-carbonyl)-pyrrolidin-3-yl]-N-methyl-3-trifluoromethyl-benzamide To a solution of 4-chloro-N-[(3RS,4SR)-4-(4-chloro-phenyl)-1-(piperidine-4-carbonyl)-pyrrolidin-3-yl]-N-methyl-3-trifluoromethyl-benzamide (216 mg, 0.382 mmol) in DMF (1 mL) was added sodium hydride (55% dispersion in mineral oil, 6 mg, 0.14 mmol). After stirring for 30 min at ambient temperature <strong>[624-75-9]iodoacetonitrile</strong> (9 mul, 0.12 mmol) was added And the suspension was stirred for 18 h at this temperature. After the addition of further <strong>[624-75-9]iodoacetonitrile</strong> (9 mul, 0.12 mmol) the resulting dark brown solution was stirred for further 5 h at ambient temperature. It was diluted with ethyl acetate (10 mL) and was washed with aqueous sodium carbonate (1 M, 10 mL) and brine (10 mL). The aqueous layers were extracted with ethyl acetate (10 mL) and the combined organic phases were dried over sodium sulfate. Purification by chromatography (SiO<SUB>2</SUB>, (ethyl acetate:triethylamine=95:5):methanol=100:0 to 70:30) afforded the title compound (45 mg, 88%) as a white foam. MS m/e: 567.1 [M]<SUP>+</SUP>. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
41.4% | EXAMPLE 7 {4-[3-(4-Chlorophenyl)-lH-pyrazolo[4,3-c]pyridin-l-yl]piperidin-l-yl}acetonitrileExample 5 (185 mg, 0.59 mmol) was dissolved in DMF (5 niL) and DIPEA (91.6 mg, 123 muL, 0.71 mmol) and <strong>[624-75-9]iodoacetonitrile</strong> (51.0 muL, 0.71 mmol) were added. The reaction mixture was heated using a Biotage microwave (100 0C, absorption high, pre-stirring 30 sec) for 10 min, and then concentrated in vacuo. The residue was dissolved in MeOH (5 mL), poured into 1 M aq Na2CO3 solution (50 mL) and extracted with EtOAc (3 x 50 mL).The combined organic layers were dried (MgSO4) and concentrated in vacuo. The residue was purified by reverse phase HPLC (YMC ODS-A 100 x 20 mm, 5 mum, 25 mL/min, gradient 60% to 100% (over 7 min) then 100% (3 min) MeOH in 10% MeOH/water) to give (4-[3-(4-chlorophenyl)- lH-pyrazolo[4,3-c]pyridin- 1 -yljpiperidin- 1 -yl} acetonitrile (86 mg, 41.4%) as a cream solid.Analytical HPLC: purity 97.9% (System B, Rtau = 4.57 min); Analytical LCMS: purity 100% (System B, Rtau = 4.61 min), ES+: 352.5 [35ClMH]+ and 354.5 [37ClMH]+; HRMS calcd for Ci9Hi8ClN5: 351.1251, found 351.1246 |
Yield | Reaction Conditions | Operation in experiment |
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The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (320 mg, 0.95 mmol), 2-acetylphenylboronic acid (0.19 g, 1.13 mmol), dichlorobis(triphenylphosphine)palladium(II) (33 mg, 0.05 mmol) and potassium carbonate (131 mg, 0.95 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (152 mg, 61%); m.p. 63-64 C.; 1H NMR (CDCl3) delta 2.19 (s, 3H), 2.28 (s, 3H), 3.70 (s, 3H), 5.16 (s, 2H), 6.75 (s, 1H), 6.79 (s, 1H), 6.90 (s, 1H), 7.11 (d, J=4.1 Hz, 1H), 7.40-7.55 (m, 4H), 7.65 (dd, J=4.8, 7.2 Hz, 1H), 7.81 (s, 1H), 8.48 (d, J=5.1 Hz, 1H). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (320 mg, 0.95 mmol), 2-acetylphenyl boronic acid (0.19 g, 1.13 mmol), dichlorobis(triphenylphosphine)palladium (II) (33 mg, 0.05 mmol) and potassium carbonate (131 mg, 0.95 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (102 mg, 82%); m.p. 58-59 C.; 1H NMR (CDCl3) delta 2.19 (s, 3H), 2.39 (s, 3H), 3.79 (s, 3H), 4.95 (s, 2H), 6.72 (s, 1H), 6.79 (s, 1H), 6.92 (s, 1H), 7.06 (d, J=4.3 Hz, 1H), 7.34 (d, J=7.5 Hz, 1H), 7.39-7.48 (m, 4H), 7.98 (s, 1H), 8.43 (d, J=5.1 Hz, 1H); 13C NMR (CDCl3) delta 21.59, 30.48, 37.80, 55.47, 112.20, 114.17, 116.92, 119.23, 119.92, 119.96, 122.67, 127.60, 128.68, 128.93, 128.98, 130.23, 138.65, 139.60, 140.59, 141.46, 141.54, 141.95, 149.48, 157.79, 160.44, 204.11. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 64 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and the residue was treated in water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of regioisomers as yellow oil. These two regioisomers (4.16 g, 80%) were used in the next reaction step without separation.Example 65-2Preparation of 4-(2-chloropyridin-5-yl)-5-(3-methoxy-5-methylphenyl)-pyrazol-1-yl)acetonitrile1H NMR (CDCl3) delta 2.28 (s, 3H), 3.66 (s, 3H), 5.16 (s, 2H), 6.71 (s, 2H), 6.84 (s, 1H), 7.24 (d, J=8.3 Hz, 1H), 7.49 (dd, J=2.3, 5.9 Hz, 1H), 7.70 (s, 1H, 8.32 (s, 1H); 13C NMR (CDCl3) delta 21.57, 39.89, 55.23, 110.80, 113.76, 115.28, 117.76, 121.47, 124.02, 127.24, 129.92, 132.57, 138.58, 140.12, 148.71, 149.92, 151.17, 159.69, 162.33.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 65 (400 mg, 1.18 mmol), 2-acetylphenyl boronic acid (023 g, 1.42 mmol), dichlorobis(triphenylphosphine)palladium (II) (41 mg, 0.06 mmol) and potassium carbonate (0.16 g, 1.18 mmol). The reaction system was purged with nitrogen gas for 10 min, and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (155 mg); m.p. 176-177 C.; 1H NMR (CDCl3) delta 2.25 (s, 3H), 2.28 (s, 3H), 3.70 (s, 3H), 5.14 (s, 2H), 6.72 (s, 1H), 6.76 (s, 1H), 6.89 (s, 1H), 7.44-7.65 (m, 6H), 7.70 (s, 1H), 8.54 (s, 1H); 13C NMR (CDCl3) delta 21.51, 30.58, 39.79, 55.19, 110.80, 113.82, 115.30, 118.66, 121.54, 121.89, 127.06, 127.63, 128.80, 128.96, 130.02, 130.36, 132.92, 136.56, 138.15, 139.93, 141.68, 148.55, 151.23, 155.76, 159.71, 204.39. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 64 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and the residue was treated in water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of regioisomers as yellow oil. These two regioisomers (4.16 g, 80%) were used in the next reaction step without separation.Example 65-2Preparation of 4-(2-chloropyridin-5-yl)-5-(3-methoxy-5-methylphenyl)-pyrazol-1-yl)acetonitrile1H NMR (CDCl3) delta 2.28 (s, 3H), 3.66 (s, 3H), 5.16 (s, 2H), 6.71 (s, 2H), 6.84 (s, 1H), 7.24 (d, J=8.3 Hz, 1H), 7.49 (dd, J=2.3, 5.9 Hz, 1H), 7.70 (s, 1H, 8.32 (s, 1H); 13C NMR (CDCl3) delta 21.57, 39.89, 55.23, 110.80, 113.76, 115.28, 117.76, 121.47, 124.02, 127.24, 129.92, 132.57, 138.58, 140.12, 148.71, 149.92, 151.17, 159.69, 162.33.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 65 (0.3 g, 0.89 mmol), 2-acetylphenylboronic acid (0.18 g, 1.07 mmol), dichlorobis(triphenylphosphine)palladium (II) (32 mg, 0.05 mmol) and potassium carbonate(0.13 g, 0.89 mmol). The reaction system was purged with nitrogen gas for 10 min, and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled to room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (72 mg); m.p. 72-73 C.; 1H NMR (CDCl3) delta 2.18 (s, 3H), 2.36 (s, 3H), 3.78 (s, 3H), 4.95 (s, 2H), 6.68 (s, 1H), 6.75 (s, 1H), 6.86 (s, 1H), 7.43-7.59 (m, 6H), 7.91 (s, 1H), 8.49 (s, 1H); 13C NMR (CDCl3) delta 21.55, 30.49, 37.81, 55.38, 112.37, 114.78, 116.72, 118.33, 122.13, 122.67, 126.78, 127.62, 128.64, 128.92, 128.99, 130.26, 134.89, 138.22, 139.49, 141.23, 141.33, 141.58, 147.50, 155.33, 160.40, 204.23. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (320 mg, 0.95 mmol), 3-acetylphenyl boronic acid (0.19 g, 1.13 mmol), thchlorobis(triphenylphosphine)palladium (II) (33 mg, 0.05 mmol) and potassium carbonate (131 mg, 0.95 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled to room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (197 mg, 79%); m.p. 73-74 C.; 1H NMR (CDCl3) delta 2.27 (s, 3H), 2.60 (s, 3H), 3.67 (s, 3H), 5.19 (s, 2H), 6.76 (s, 1H), 6.80 (s, 1H), 6.91 (s, 1H), 7.13 (s, 1H), 7.49 (bs, 1H), 7.64 (s, 1H), 7.85 (s, 1H), 7.95 (d, J=5.4 Hz, 1H), 8.04 (d, J=5.7 Hz, 1H), 8.39 (s, 1H), 8.56 (s, 1H); 13C NMR (CDCl3) delta 21.51, 26.78, 39.89, 55.23, 111.03, 113.93, 115.51, 119.76, 119.90, 121.63, 121.77, 126.71, 128.60, 128.76, 129.11, 130.54, 131.45, 132.06, 132.92, 137.52, 139.61, 140.00, 141.09, 149.91, 151.38, 156.47, 159.69, 198.16. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (320 mg, 0.95 mmol), 3-acetylphenylboronic acid (0.19 g, 1.13 mmol), dichlorobis(triphenylphosphine)palladium (II) (33 mg, 0.05 mmol) and potassium carbonate (131 mg, 0.95 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled to room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (96 mg, 77%); m.p. 65-66 C.; 1H NMR (CDCl3) delta 2.39 (s, 3H), 2.62 (s, 3H), 3.79 (s, 3H), 4.96 (s, 2H), 6.74 (s, 1H), 6.80 (s, 1H), 6.95 (s, 1H), 7.10 (bs, 1H), 7.50 (t, J=7.5 Hz, 1H), 7.56 (s, 1H), 7.94-8.00 (m, 3H), 8.29 (s, 1H), 8.52 (d, J=4.2 Hz, 1H); 13C NMR (CDCl3) delta 21.59, 26.78, 37.77, 55.78, 112.33, 114.14, 116.93, 118.18, 119.32, 120.14, 122.64, 126.60, 128.68, 129.01, 131.39, 137.53, 139.59, 139.72, 140.66, 141.54, 141.93, 150.05, 156.54, 160.45, 197.89. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 64 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and the residue was treated in water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of regioisomers as yellow oil. These two regioisomers (4.16 g, 80%) were used in the next reaction step without separation.Example 65-2Preparation of 4-(2-chloropyridin-5-yl)-5-(3-methoxy-5-methylphenyl)-pyrazol-1-yl)acetonitrile1H NMR (CDCl3) delta 2.28 (s, 3H), 3.66 (s, 3H), 5.16 (s, 2H), 6.71 (s, 2H), 6.84 (s, 1H), 7.24 (d, J=8.3 Hz, 1H), 7.49 (dd, J=2.3, 5.9 Hz, 1H), 7.70 (s, 1H, 8.32 (s, 1H); 13C NMR (CDCl3) delta 21.57, 39.89, 55.23, 110.80, 113.76, 115.28, 117.76, 121.47, 124.02, 127.24, 129.92, 132.57, 138.58, 140.12, 148.71, 149.92, 151.17, 159.69, 162.33.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 65 (320 mg, 0.95 mmol), 3-acetylphenylboronic acid (0.19 g, 1.13 mmol), dichlorobis(triphenylphosphine)palladium (II) (33 mg, 0.04 mmol) and potassium carbonate (0.13 g, 0.95 mmol). The reaction system was purged with nitrogen gas for 10 min, and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (202 mg); m.p. 73-74 C.; 1H NMR (CDCl3) delta 2.27 (s, 3H), 2.65 (s, 3H), 3.66 (s, 3H), 5.16 (s, 2H), 6.71 (s, 1H), 6.77 (s, 1H), 6.91 (s, 1H), 7.54 (t, J=7.7 Hz, 1H), 7.60-7.72 (m, 3H), 8.97 (d, J=7.5 Hz, 1H), 8.19 (d, J=7.5 Hz, 1H), 8.58 (s, 1H), 8.63 (s, 1H); 13C NMR (CDCl3) delta 21.55, 26.83, 39.84, 55.19, 110.91, 113.92, 115.23, 118.77, 120.03, 121.58, 126.53, 127.18, 128.77, 129.14, 129.93, 131.22, 133.02, 136.57, 137.63, 139.31, 139.97, 149.01, 151.22, 154.56, 159.68, 198.12. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 64 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and the residue was treated in water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of regioisomers as yellow oil. These two regioisomers (4.16 g, 80%) were used in the next reaction step without separation.Example 65-2Preparation of 4-(2-chloropyridin-5-yl)-5-(3-methoxy-5-methylphenyl)-pyrazol-1-yl)acetonitrile1H NMR (CDCl3) delta 2.28 (s, 3H), 3.66 (s, 3H), 5.16 (s, 2H), 6.71 (s, 2H), 6.84 (s, 1H), 7.24 (d, J=8.3 Hz, 1H), 7.49 (dd, J=2.3, 5.9 Hz, 1H), 7.70 (s, 1H, 8.32 (s, 1H); 13C NMR (CDCl3) delta 21.57, 39.89, 55.23, 110.80, 113.76, 115.28, 117.76, 121.47, 124.02, 127.24, 129.92, 132.57, 138.58, 140.12, 148.71, 149.92, 151.17, 159.69, 162.33.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 65 (0.32 g, 0.95 mmol), 3-acetylphenylboronic acid (0.19 g, 1.13 mmol), dichlorobis(triphenylphosphine)palladium (II) (33 mg, 0.04 mmol) and potassium carbonate (0.13 g, 0.95 mmol). The reaction system was purged with nitrogen gas for 10 min, and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (106 mg); m.p. 149-150 C.; 1H NMR (CDCl3) delta 2.37 (s, 3H), 2.67 (s, 3H), 3.78 (s, 3H), 4.95 (s, 2H), 6.71 (s, 1H), 6.77 (s, 1H), 6.87 (s, 1H), 7.51-7.70 (m, 3H), 7.92 (s, 1H), 7.97 (d, J=7.6 Hz, 1H), 8.16 (d, J=7.8 Hz, 1H), 8.53 (s, 1H), 8.57 (d, J=1.2 Hz, 1H); 13C NMR (CDCl3) delta 21.61, 26.82, 37.80, 55.41, 112.41, 114.22, 116.66, 118.39, 120.33, 122.71, 126.53, 126.96, 128.67, 129.04, 129.09, 131.18, 135.01, 137.58, 139.36, 139.48, 141.15, 141.36, 147.96, 154.21, 160.37, 198.06. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (320 mg, 0.95 mmol), 4-acetylphenyl boronic acid (0.19 g, 1.13 mmol), dichlorobis(triphenylphosphine)palladium (II) (33 mg, 0.05 mmol) and potassium carbonate (131 mg, 0.95 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (170 mg, 68%); m.p. 99-100 C.; 1H NMR (CDCl3) delta 2.32 (s, 3H), 2.65 (s, 3H), 3.72 (s, 3H), 5.20 (s, 2H), 6.79 (s, 1H), 6.82 (s, 1H), 6.94 (s, 1H), 7.20 (d, J=5.1 Hz, 1H), 7.69 (s, 1H), 7.88 (s, 1H), 7.95 (d, J=8.4 Hz, 2H), 8.02 (d, J=8.4 Hz, 2H), 8.63 (d, J=5.1 Hz, 1H); 13C NMR (CDCl3) delta 21.54, 26.80, 39.95, 55.27, 110.97, 113.53, 115.58, 120.20, 121.74, 121.88, 127.00, 128.82, 130.02, 132.77, 137.19, 140.06, 140.95, 143.39, 150.15, 151.54, 156.35, 159.77, 197.91. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (320 mg, 0.95 mmol), 4-acetylphenyl boronic acid (0.19 g, 1.13 mmol), dichlorobis(triphenylphosphine)palladium (II) (33 mg, 0.05 mmol) and potassium carbonate (131 mg, 0.95 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (93 mg, 75%); m.p. 156-157 C.; 1H NMR (CDCl3) delta 2.42 (s, 3H), 2.65 (s, 3H), 3.82 (s, 3H), 4.97 (s, 2H), 6.75 (s, 1H), 6.82 (s, 1H), 6.96 (s, 1H), 7.12 (d, J=4.9 Hz, 1H), 7.60 (s, 1H), 7.87 (d, J=8.1 Hz, 2H), 7.99-8.02 (m, 3H), 8.56 (d, J=5.1 Hz, 1H); 13C NMR (CDCl3) delta 21.60, 26.78, 37.78, 55.52, 112.34, 114.02, 116.86, 118.62, 119.33, 120.38, 122.67, 126.90, 128.76, 129.01, 137.12, 139.56, 140.61, 141.58, 141.96, 143.49, 150.20, 156.29, 160.54, 197.88. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of acetonitrile and water (4:1, 10 mL) were added the mixture prepared in Example 22 (100 mg, 0.30 mmol), 3-pyridineboronic acid (40.5 mg, 0.33 mmol), dichlorobis(triphenylphosphine)palladium (II) (10 mg, 0.015 mmol) and potassium carbonate (41 mg, 0.295 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 2:1, v/v): (180 mg, 80%); m.p. 72-73 C.; 1H NMR (CDCl3) delta 2.28 (s, 3H), 3.69 (s, 3H), 5.17 (s, 2H), 6.76 (s, 1H), 6.80 (s, 1H), 6.91 (s, 1H), 7.17 (dd, J=1.2, 3.9 Hz, 1H), 7.35 (dd, J=2.7, 4.8 Hz, 1H), 7.62 (s, 1H), 7.84 (s, 1H), 8.18 (d, J =8.1 Hz, 1H), 8.56-8.60 (m, 2H), 9.00 (d, J=1.3 Hz, 1H) 13C NMR (CDCl3) delta 21.50, 39.91, 55.25, 111.12, 113.66, 115.49, 119.72, 119.98, 121.72, 121.80, 123.61, 130.20, 132.77, 134.33, 134.72, 140.03, 141.06, 148.11, 149.94, 150.25, 151.48, 155.00, 159.75. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (300 mg, 0.89 mmol), 3-pyridineboronic acid (0.13 g, 1.06 mmol), dichlorobis(triphenylphosphine)palladium (II) (31 mg, 0.04 mmol) and potassium carbonate (130 mg, 0.89 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 2:1, v/v): (62 mg, 55%); m.p. 81-82 C.; 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.80 (s, 3H), 4.95 (s, 2H), 6.73 (s, 1H), 6.80 (s, 1H), 6.94 (s, 1H), 7.11 (d, J=5.0 Hz, 1H), 7.34 (dd, J=3.0, 4.8 Hz, 1H), 7.54 (s, 1H), 8.00 (s, 1H), 8.15 (d, J=7.8 Hz, 1H), 8.54 (d, J=5.1 Hz, 1H), 8.60 (d, J=4.4 Hz, 1H), 8.89 (s, 1H); 13C NMR (CDCl3) delta 21.62, 37.79, 55.50, 112.38, 114.08, 116.81, 118.15, 119.21, 120.35, 122.59, 123.59, 128.90, 134.30, 134.78, 139.57, 140.71, 141.57, 141.97, 148.02, 149.92, 150.34, 154.99, 160.48. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 64 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and the residue was treated in water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of regioisomers as yellow oil. These two regioisomers (4.16 g, 80%) were used in the next reaction step without separation.Example 65-2Preparation of 4-(2-chloropyridin-5-yl)-5-(3-methoxy-5-methylphenyl)-pyrazol-1-yl)acetonitrile1H NMR (CDCl3) delta 2.28 (s, 3H), 3.66 (s, 3H), 5.16 (s, 2H), 6.71 (s, 2H), 6.84 (s, 1H), 7.24 (d, J=8.3 Hz, 1H), 7.49 (dd, J=2.3, 5.9 Hz, 1H), 7.70 (s, 1H, 8.32 (s, 1H); 13C NMR (CDCl3) delta 21.57, 39.89, 55.23, 110.80, 113.76, 115.28, 117.76, 121.47, 124.02, 127.24, 129.92, 132.57, 138.58, 140.12, 148.71, 149.92, 151.17, 159.69, 162.33.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 65 (0.3 g, 0.89 mmol), 3-pyridineboronic acid (0.13 g, 1.07 mmol), dichlorobis(triphenylphosphine)palladium (II) (32 mg, 0.05 mmol) and potassium carbonate (0.13 g, 0.89 mmol). The reaction system was purged with nitrogen gas for 10 min, and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:3, v/v): (176 mg); m.p. 66-67 C.; 1H NMR (CDCl3) delta 2.27 (s, 3H), 3.67 (s, 3H), 5.16 (s, 2H), 6.71 (s, 1H), 6.76 (s, 1H), 6.90 (s, 1H), 7.36-7.40 (m, 1H), 7.62-7.69 (m, 2H), 7.73 (s, 1H), 8.29 (d, J=7.7 Hz, 1H), 8.62-8.65 (m, 2H), 9.19 (s, 1H); 13C NMR (CDCl3) delta 21.51, 39.85, 55.18, 110.91, 113.73, 115.30, 118.78, 119.96, 121.57, 123.62, 127.41, 129.76, 132.92, 134.10, 134.35, 136.58, 139.97, 148.09, 149.28, 149.93, 151.30,153.13, 159.71. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 64 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and the residue was treated in water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of regioisomers as yellow oil. These two regioisomers (4.16 g, 80%) were used in the next reaction step without separation.Example 65-2Preparation of 4-(2-chloropyridin-5-yl)-5-(3-methoxy-5-methylphenyl)-pyrazol-1-yl)acetonitrile1H NMR (CDCl3) delta 2.28 (s, 3H), 3.66 (s, 3H), 5.16 (s, 2H), 6.71 (s, 2H), 6.84 (s, 1H), 7.24 (d, J=8.3 Hz, 1H), 7.49 (dd, J=2.3, 5.9 Hz, 1H), 7.70 (s, 1H, 8.32 (s, 1H); 13C NMR (CDCl3) delta 21.57, 39.89, 55.23, 110.80, 113.76, 115.28, 117.76, 121.47, 124.02, 127.24, 129.92, 132.57, 138.58, 140.12, 148.71, 149.92, 151.17, 159.69, 162.33.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 65 (0.3 g, 0.89 mmol), 3-pyridineboronic acid (0.13 g, 1.07 mmol), dichlorobis(triphenylphosphine)palladium (II) (32 mg, 0.05 mmol) and potassium carbonate (0.13 g, 0.89 mmol). The reaction system was purged with nitrogen gas for 10 min, and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled to room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:3, v/v): (87 mg); m.p. 64-65 C.; in NMR (CDCl3) delta 2.37 (s, 3H), 3.79 (s, 3H), 4.94 (s, 2H), 6.71 (s, 1H), 6.77 (s, 1H), 6.88 (s, 1H), 7.36-7.40 (m, 1H), 7.60-7.67 (m, 2H), 7.92 (s, 1H), 8.27 (d, J=7.7 Hz, 1H), 8.60-8.62 (m, 2H), 9.15 (s, 1H); 13C NMR (CDCl3) delta 21.60, 37.81, 55.41, 112.42, 114.14, 116.71, 118.30, 120.26, 122.69, 123.62, 127.25, 128.99, 134.09, 134.43, 135.01, 139.48, 141.21, 141.39, 147.98, 148.25, 149.80, 152.64, 160.40. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (300 mg, 0.89 mmol), phenylboronic acid (0.12 g, 0.974 mmol), dichlorobis(triphenylphosphine)palladium (II) (31 mg, 0.044 mmol) and potassium carbonate (130 mg, 0.89 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:3, v/v): (166 mg, 74%); m.p. 55-56 C; 1H NMR (CDCl3) delta 2.32 (s, 3H), 3.71 (s, 3H), 5.14 (s, 2H), 6.79 (s, 1H), 6.84 (s, 1H), 6.96 (s, 1H), 7.13 (d, J=4.9 Hz, 1H), 7.41-7.48 (m, 3H), 7.65 (s, 1H), 7.79 (s, 1H), 7.88 (d, J=6.9 Hz, 2H), 8.61 (d, J=5.1 Hz, 1H); 13C NMR (CDCl3) delta21.51, 39.86, 55.25, 110.90, 113.61, 115.61, 119.78, 120.42, 121.24, 121.76, 126.89, 128.78, 129.11, 130.05, 132.90, 139.19, 139.98, 140.77, 149.89, 151.46, 157.75, 159.75. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (100 mg, 0.30 mmol), phenylboronic acid (43.9 mg, 0.36 mmol), dichlorobis(triphenylphosphine)palladium (II) (11 mg, 0.02 mmol) and potassium carbonate (42 mg, 0.30 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled at room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:3, v/v): (70 mg, 62%); m.p. 51-52 C.; 1H NMR (CDCl3) delta 2.41 (s, 3H), 3.81 (s, 3H), 4.95 (s, 2H), 6.76 (s, 1H), 6.82 (s, 1H), 6.95 (s, 1H), 7.06 (d, J=4.5 Hz, 1H), 7.41-7.44 (m, 3H), 7.58 (s, 1H), 7.80 (d, J=6.3 Hz, 2H), 8.01 (s, 1H), 8.53 (d, J=4.8 Hz, 1H) 13C NMR (CDCl3) delta 21.57, 37.76, 55.49, 112.31, 114.07, 116.90, 118.29, 119.60, 119.70, 122.75, 126.83, 128.71, 129.03, 129.11, 139.21, 139.61, 140.45, 141.47, 141.89, 149.88, 157.70, 160.51. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
The compound obtained in Example 21 (4.6 g, 15.35 mmol) and potassium carbonate (10.6 g, 76.73 mmol) were added to acetone (100 mL) and refluxed for 2 hours by heating. To this reaction mixture was dropwise added <strong>[624-75-9]iodoacetonitrile</strong> (1.34 mL, 18.42 mmol), and refluxed over 2 hours. The acetone was removed by vacuum distillation, and to the residue were added water (200 mL) and ethyl acetate (200 mL). The organic layer was dried over anhydrous magnesium sulfate and distillated in a vacuum. The concentrate was subjected to column chromatography (silica gel, ethyl acetate-hexane 2:3 v/v) to afford a mixture of 2:1 of [4-(2-chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile and [4-(2-chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile as yellow oil. These two regioisomers (4.78 g, 92%) were used in the next reaction step without separation.[4-(2-Chloropyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.29 (s, 3H), 3.71 (s, 3H), 5.19 (s, 2H), 6.73 (s, 1H), 6.75 (s, 1H), 6.85 (s, 1H), 7.06 (d, J=4.4 Hz, 1H), 7.26 (s, 1H), 7.79 (s, 1H), 8.24 (d, J=4.8 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.97, 55.29, 110.91, 111.12, 113.65, 115.67, 118.75, 121.59, 121.65, 122.78, 130.58, 132.37, 140.16, 143.28, 149.68, 151.45, 151.79, 159.71, 162.33.[4-(2-Chloropyridin-4-yl)-5-(3-methoxy-5-methylphenyl)-1H-pyrazol-1-yl]acetonitrile: 1H NMR (CDCl3) delta 2.39 (s, 3H), 3.83 (s, 3H), 4.94 (s, 2H), 6.63 (s, 1H), 6.74 (s, 1H), 6.92-6.96 (m, 2H), 7.19 (s, 1H), 7.93 (s, 1H), 8.17 (d, J=5.2 Hz, 1H); 13C NMR (CDCl3) delta 21.56, 37.78, 55.48, 112.15, 113.91, 117.08, 118.16, 119.88, 121.36, 122.48, 128.46, 139.57, 141.60, 142.19, 142.85, 149.78, 151.95, 160.48.To a solvent mixture of THF and water (4:1, 10 mL) were added the mixture prepared in Example 22 (320 mg, 0.95 mmol), 4-(dimethylaniino)phenylboronic acid (0.19 g, 1.13 mmol), dichlorobis(triphenylphosphine)palladium (II) (34 mg, 0.05 mmol) and potassium carbonate (131 mg, 0.95 mmol), and stirred at 70 C. for 12 hours under nitrogen atmosphere. The reaction mixture was cooled to room temperature, washed with ice water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic extract was dried over anhydrous magnesium sulfate and distilled under vacuum. The residue was subjected to prep-TLC using a solvent mixture of ethyl acetate/hexane to purify the desired products.Purification yield by prep-TLC (silica gel, ethyl acetate-hexane, 1:2, v/v): (197 mg, 79%); m.p. 98-99 C.; 1H NMR (CDCl3) delta 2.31 (s, 3H), 3.01 (s, 6H), 3.70 (s, 3H), 5.10 (s, 2H), 6.75-6.78 (m, 3H), 6.84 (s, 1H), 6.97-7.00 (m, 2H), 7.56 (s, 1H), 7.72 (s, 1H), 7.80 (d, J=8.8 Hz, 2H), 8.52 (d, J=5.0 Hz, 1H); 13C NMR (CDCl3) delta 21.53, 39.79, 40.34, 55.25, 110.68, 112.16, 113.70, 115.67, 118.33, 119.88, 120.69, 121.74, 126.77, 127.72, 130.10, 132.99, 139.89, 140.46, 149.55, 151.13, 151.35, 157.78, 159.68. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
37.4% | With potassium carbonate; In N,N-dimethyl-formamide; at 50℃; for 16h; | In a two-necked round-bottom flask, [3-(3-methoxy-5-methylphenyl)-4-(2-methylthiopyrimidine-4-yl]-1H-pyrazol (5 g, 16.0 mmol) was dissolved in dimethylformamide (65 mL) to which potassium carbonate (5.52 g, 40 mmol) and <strong>[624-75-9]iodoacetonitrile</strong> (2.9 mL, 40 mmol) were added, and heated at 50 C. for 16 hours with stirring. After completion of the reaction, the reaction mixture was washed with brine and extracted with ethyl acetate. The organic layer was washed with brine and dried over anhydrous magnesium sulfate. The solvent was removed by vacuum distillation and the residue was purified through column chromatography to recover the title compound (2.1 g, 37.4%).m.p. 117 C.; 1H NMR (400 MHz, CDCl3) delta 2.36 (s, 3H), 2.54 (s, 3H), 3.78 (s, 3H), 5.15 (s, 2H), 6.79 (s, 1H), 6.80 (s, 1H), 6.82 (d, J=4.79 Hz, 1H), 6.92 (s, 1H), 8.26 (s, 1H), 8.30 (d, J=5.22 Hz, 1H); IR (KBr) 3429, 2926, 1571, 1460, 1324, 1157 cm-1. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
67% | With caesium carbonate; In N,N-dimethyl-formamide; at 110℃; for 42h; | As shown in step 5-ii of Scheme 5, 5-bromo-2-(lH-pyrazol-4-yl)isoindolin-l-one (1.2 g, 4.32 mmol) was combined with cesium carbonate (1.69 g, 5.18 mmol) in DMF (10 mL) in a sealable tube and nitrogen gas was bubbled through the solution for 5 minutes. 2- <strong>[624-75-9]Iodoacetonitrile</strong> (1.08 g, 468 mu, 6.47 mmol) was added and the tube was sealed and heated to 110 C in an oil bath for 18 hours. Additional <strong>[624-75-9]iodoacetonitrile</strong> added (0.5 mL) and the reaction mixture was heated for an additional 24 hours. The reaction mixture was poured into H20/EtOAc and the resulting dark brown solid was collected by filtration. The solid was washed with MeOH and then diethyl ether to provide 2-[4-(5-bromo-l-oxo-isoindolin-2- yl)pyrazol-l-yl]acetonitrile (Compound 2020, 920 mg, 2.9 mmol, 67% yield): ESMS (M+H) 319.04; 1H NMR (DMSO-d6) delta 8.35 (s, 1H), 7.92 (m, 2H), 7.70 (m, 2H), 5.53 (s, 2H), 4.88 (s, 2H). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
78% | With ferrous(II) sulfate heptahydrate; dihydrogen peroxide; at 20 - 35℃; | A 500-mL three-neck round-bottomed flask equipped with an addition funnel, thermometer and charged with 118a (6.70 g, 37.4 mmol), <strong>[624-75-9]Iodoacetonitrile</strong> (12.5 g, 74.9 mmol), iron (II) sulfate heptahydrate (5.20 g, 18.7 mmol) and dimethyl sulfoxide (250 mL). Hydrogen peroxide (35%, 18.2 g, 187 mmol) was added dropwise to the mixture in 1 h through a syringe pump at room temperature using a water bath. Iron (II) sulfateheptahydrate (2 to 3 equivalent) was added to the reaction mixture in portions to keep the temperature between 25 C to 35 C, until the color of the reaction mixture is deep red. If TLC show the reaction not completed, then more hydrogen peroxide (2-3 equivalent) and more iron (II) sulfate heptahydrate (1-2 equivalent) were added in the same manner until the reaction is completed. After that time, the reaction mixture was partitioned between saturated sodium bicarbonate solution (200 mL) and ethyl acetate (400 mL). The organic layer was separated, and the aqueous layer was extracted with ethyl acetate (2 x 100 mL). The combined organic layers were washed with saturated Sodium thiosulfate solution (50 mL), dried over sodium sulfate and concentrated under reduced pressure. The residue was purified by column chromatography to afford a 78% yield (6.40 g) of 118b as a yellow oil: ]H NMR (500 MHz, CDC13) delta 6.23 (s, 1H), 4.23 (s, 2H), 3.94 (t, 2H, J = 6.5 Hz), 3.81 (s, 3H), 2.74 (t, 2H, J = 6.5 Hz), 2.00 (m, 2H), 1.83 (m, 2H); (APCI+) m/z 219.3 (M+H) |
78% | With ferrous(II) sulfate heptahydrate; dihydrogen peroxide; at 20 - 35℃; | Example 101e Methyl 3-(Cyanomethyl)-5,6,7,8-tetrahydroindolizine-2-carboxylate 101e A 500-mL three-neck round-bottomed flask equipped with an addition funnel, thermometer and charged with methyl 5,6,7,8-tetrahydroindolizine-2-carboxylate 101d (6.70 g, 37.4 mmol), <strong>[624-75-9]iodoacetonitrile</strong> (12.5 g, 74.9 mmol), iron (II) sulfate heptahydrate (5.20 g, 18.7 mmol) and dimethyl sulfoxide (250 mL). Hydrogen peroxide (35%, 18.2 g, 187 mmol) was added dropwise to the mixture in 1 h through a syringe pump at room temperature using a water bath. Iron (II) sulfate heptahydrate (2 to 3 equivalent) was added to the reaction mixture in portions to keep the temperature between 25 C. to 35 C., until the color of the reaction mixture is deep red. If TLCs show the reaction not completed, then more hydrogen peroxide (2-3 equivalent) and more iron (II) sulfate heptahydrate (1-2 equivalent) were added in the same manner until the reaction is completed. After that time, the reaction mixture was partitioned between aqueous saturated sodium bicarbonate solution (200 mL) and ethyl acetate (400 mL). The organic layer was separated, and the aqueous layer was extracted with ethyl acetate (2*100 mL). The combined organic layers were washed with saturated Sodium thiosulfate solution (50 mL), dried over sodium sulfate and concentrated under reduced pressure. The residue was purified by column chromatography to afford a 78% yield (6.40 g) of 101e as a yellow oil: 1H NMR (500 MHz, CDCl3) delta 6.23 (s, 1H), 4.23 (s, 2H), 3.94 (t, 2H, J=6.5 Hz), 3.81 (s, 3H), 2.74 (t, 2H, J=6.5 Hz), 2.00 (m, 2H), 1.83 (m, 2H); (APCI+) m/z 219.3 (M+H) |
78% | With ferrous(II) sulfate heptahydrate; dihydrogen peroxide; In dimethyl sulfoxide; at 20 - 35℃; | A 500-mL three-neck round-bottomed flask equipped with an addition funnel, thermometer and charged with 120a (6.70 g, 37.4 mmol), <strong>[624-75-9]iodoacetonitrile</strong> (12.5 g, 74.9 mmol), iron (II) sulfate heptahydrate (5.20 g, 18.7 mmol) and dimethyl sulfoxide (250 mL). Hydrogen peroxide (35%, 18.2 g, 187 mmol) was added dropwise to the mixture over the period of 1 h through a syringe pump at room temperature using a water bath. Iron (II) sulfate heptahydrate (2 to 3 equivalent) was added to the reaction mixture in portions to keep the temperature between 25 C. to 35 C., until the color of the reaction mixture was deep red. When TLCs showed the reaction was not complete, more hydrogen peroxide (2-3 equivalent) and more iron (II) sulfate heptahydrate (1-2 equivalents) were added in the same manner until the reaction was complete. After that time, the reaction mixture was partitioned between saturated sodium bicarbonate solution (200 mL) and ethyl acetate (400 mL). The organic layer was separated, and the aqueous layer was extracted with ethyl acetate (2*100 mL). The combined organic layers were washed with saturated sodium thiosulfate solution (50 mL), dried over sodium sulfate and concentrated under reduced pressure. The residue was purified by column chromatography to afford a 78% yield (6.40 g) of 120b as a yellow oil: 1H NMR (500 MHz, CDCl3) delta 6.23 (s, 1H), 4.23 (s, 2H), 3.94 (t, 2H, J=6.5 Hz), 3.81 (s, 3H), 2.74 (t, 2H, J=6.5 Hz), 2.00 (m, 2H), 1.83 (m, 2H); (APCI+) m/z 219.3 (M+H) |
78% | With ferrous(II) sulfate heptahydrate; dihydrogen peroxide; In dimethyl sulfoxide; at 25 - 35℃; | Example 112b Methyl 3-(Cyanomethyl)-5,6,7,8-tetrahydroindolizine-2-carboxylate 112b A 500-mL three-neck round-bottomed flask equipped with an addition funnel, thermometer and charged with 112a (6.70 g, 37.4 mmol), <strong>[624-75-9]Iodoacetonitrile</strong> (12.5 g, 74.9 mmol), iron (II) sulfate heptahydrate (5.20 g, 18.7 mmol) and dimethyl sulfoxide (250 mL). Hydrogen peroxide (35%, 18.2 g, 187 mmol) was added dropwise to the mixture in 1 h through a syringe pump at room temperature using a water bath. Iron (II) sulfate heptahydrate (2 to 3 equivalent) was added to the reaction mixture in portions to keep the temperature between 25 C to 35 C, until the color of the reaction mixture is deep red. If TLC shows the reaction not completed, then more hydrogen peroxide (2-3 equivalent) and more iron (II) sulfate heptahydrate (1-2 equivalent) are added in the same manner until the reaction is completed. After that time, the reaction mixture was partitioned between saturated sodium bicarbonate solution (200 mL) and ethyl acetate (400 mL). The organic layer was separated, and the aqueous layer was extracted with ethyl acetate (2 * 100 mL). The combined organic layers were washed with saturated Sodium thiosulfate solution (50 mL), dried over sodium sulfate and concentrated under reduced pressure. The residue was purified by column chromatography to afford a 78% yield (6.40 g) of 112b as a yellow oil: 1H NMR (500 MHz, CDCl3) delta 6.23 (s, 1H), 4.23 (s, 2H), 3.94 (t, 2H, J = 6.5 Hz), 3.81 (s, 3H), 2.74 (t, 2H, J = 6.5 Hz), 2.00 (m, 2H), 1.83 (m, 2H); (APCI+) m/z 219.3 (M+H) |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
78% | With 18-crown-6 ether; potassium carbonate; In N,N-dimethyl-formamide; at 20℃; for 12h; | Example 41 Benzyl {(7R)-6-cyano-7-(4-cyanophenyl)-5-methyl-4-[3-(trifluoromethyl)phenyl]-4,7-dihydro-[1,2,4]triazolo[1,5-a]pyrimidin-2-yl}(cyanomethyl)carbamate Potassium carbonate (15.7 mg, 113 mmol, 2.1 eq.), 18-crown-6 (30 mg, 113 mmol, 2.1 eq.) and <strong>[624-75-9]iodoacetonitrile</strong> (19 mg, 113 mmol, 2.1 eq.) were added to a solution of benzyl {(7R)-6-cyano-7-(4-cyanophenyl)-5-methyl-4-[3-(trifluoromethyl)phenyl]-4,7-dihydro[1,2,4]triazolo[1,5-a]pyrimidin-2-yl}carbamate (30 mg, 54 mmol) in DMF (2 ml). The reaction mixture was stirred at RT for 12 h and then concentrated under reduced pressure. The residue was acidified with acetic acid and then purified by preparative HPLC (Kromasil C18 column, 5 mum, 50*20 mm; mobile phase: acetonitrile-water-0.1% TFA). After lyophilization, the product was obtained as a solid (25 mg, 78% of theory). LC-MS (Method 5): Rt=1.40 min; MS (ESIpos): m/z (%)=434.0 (100), 550.9 (20), 595.0 (30) [M+H]+; MS (ESIneg): m/z (%)=593.8 (100) [M-H]-. 1H-NMR (400 MHz, DMSO-d6): delta=2.00 (s, 3H), 4.55 (d, 2H), 5.10 (d, 2H), 6.55 (s, 1H), 7.15-7.35 (m, 5H), 7.70-7.95 (m, 7H), 8.15 (br. s, 1H). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
78% | With potassium carbonate; In N,N-dimethyl-formamide; acetone; for 6h;Reflux; | Thymidine (10 g, 41.3 mmol) and potassium carbonate (17 g, 123 mmol) were dissolved in acetone/ DMF (100 mL, 1/1, v/v) and iodoacetonitile (6.5 mL, 90 mmol) was added. The reaction mixture was refluxed for 6 h, then added to water (750 mL), and extracted with ethyl acetate (250 mL × 4). The organic layer was separated, dried using anhydrous magnesium sulphate (100 g), evaporated under reduced pressure, and purified by silica gel column chromatography using dichloromethane and methanol as the solvent system. The compound was eluted at 4% methanol. Rf = 0.70 (10% methanol in dichloromethane), yield = 9.1 g, 78% as yellow solid, (m.p. 52 C); 1H NMR (400 MHz, CD3OD, delta ppm): 7.86 (s, 1H, H-6), 6.25 (t, J = 6.6 Hz, 1H, 1?H), 4.80 (s, 2H, CH2-CN), 4.33-4.41 (m, 1H, 3?H), 3.87-3.95 (m, 1H, 4?H), 3.78 (dd, J = 2.8 and 12.0 Hz, 2H, 5?H), 3.70 (dd, J = 3.1 and 12.0 Hz, 2H, 5?H), 2.23-2.33 (m, 1H, 2?H), 2.12-2.22 (m, 1H, 2?H), 1.89 (s, 3H, 5-CH3). 13C NMR (CD3OD, 100 MHz, delta ppm): 163.29 (C-4 C=O), 150.62 (C-2 C=O), 136.77 (C-6), 115.46 (CN), 110.30 (C-5), 88.27 (C-1?), 86.79 (C-4?), 71.40 (C-3?), 62.16 (C-5?), 41.09 (C-2?), 28.92 (N3-CH2-CN), 13.05 (5-CH3); HR-MS (ESI-MS) for C12H15N3NaO5. Calcd: 304.0909. Found: m/z 304.0888 (M + Na)+. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
89% | With N-ethyl-N,N-diisopropylamine; In chloroform; at 20 - 25℃; for 23h; | <strong>[624-75-9]Iodoacetonitrile</strong> (4.30 mul, 0.059 mmol) was added to a solution of 8-chloro-3-(5-chloro-2-ethanesulfonyl-benzyl)-7-piperazin-1-ylmethyl-6-trifluoromethyl-1H-quinazoline-2,4-dione (Compound F-1, 30.8 mg, 0.053 mmol) and DIPEA (10.2 mul, 0.059 mmol) in chloroform (0.265 ml), and the mixture was stirred at room temperature for 8.5 hours. DIPEA (8.33 mul, 0.048 mmol), <strong>[624-75-9]iodoacetonitrile</strong> (3.46 mul, 0.048 mmol), and chloroform (0.265 ml) were further added to this reaction solution, and the mixture was stirred at room temperature for 14.5 hours. Ethyl acetate (15 ml) and THF (15 ml) were added to the reaction mixture, and the organic layer was washed with a saturated aqueous ammonium chloride solution and brine, and dried over anhydrous sodium sulfate. The drying agent was removed by filtration, and the residue obtained by concentration under reduced pressure was purified by amino silica gel column chromatography (MeOH/DCM) to give the title compound (29.3 mg, 89%) as a colorless amorphous. LCMS: m/z 618 [M+H]+ HPLC retention time: 1.77 min (analysis condition C) |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
59% | General procedure: A 1.5 mL (1.5 mmol) ofPhBCl2 (1.0 mol/L in CH2Cl2) was added to CH2Cl2 solution(4 mL) of the nitrile (5a) (173 mg, 1.2 mmol) at room temperatureunder argon. The mixture was stirred for 15 min. Theindole (1a) (131 mg, 1.0 mmol) was added dropwise to this solutionat room temperature. The resulting solution was stirredfor 3 h, and then 0.5 mol/L Na2CO3 was added to quench thereaction. The resulting mixture was extracted with CH2Cl2.The organic layer was washed with saturated brine, dried(MgSO4), and evaporated. The crude product was purifiedusing silica gel chromatography to provide 3aa (226 mg, 82%yield). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
95% | With potassium carbonate; In dimethyl sulfoxide; acetonitrile; at 60℃; for 3h; | Step 2: Synthesis of 2-(2-chloro-6-morpholino-9H-purin-9-yl)acetonitrile (14c)To a solution of 13a (1.18 g, 4.94 mmol) in acetonitrile/DMSO (19:1) was added 2- <strong>[624-75-9]iodoacetonitrile</strong> (0.71 mL, 9.87 mmol) and K2C03 (1.36 g, 9.87 mmol). The resulting mixture was heated at 60CC for 3 h. Then the solvents were removed in vacuo and water was added. The aqueous layer was extracted with DCM (x2) and the combined organic layers was washed with brine (xl), dried over MgSO4 and evaporated in vacuo. The crude oil was purified by flash chromatography(silica, 50% ethyl acetate in hexanes) to afford 14c (1.31 g, 95%) as pale brown solid. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
44% | With rongalite; In water; N,N-dimethyl-formamide; at 80℃; for 6h; | General procedure: 1 (0.5mmol), 2 (1.0mmol), HOCH2SO2Na·2H2O (231mg, 1.5mmol) and a stir bar were added to 25mL tube and then DMF (3mL) and H2O (3mL) were added. The mixture was allowed to stir at 80C for 6h under air (monitored by TLC). The solution was then diluted by ethyl acetate (20mL) and washed by a solution of K2CO3 and water, then dried by anhydrous Na2SO4. The crude mixture was purified by column chromatography on silica gel (petroleum ether/ethyl acetate=15:1-5:1) to give the product 3. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
93% | General procedure: A solution of Et3B (0.5 mL, 1.0 M in EtOH, 0.5 mmol) was added over 2 h by syringe pump to a stirred mixture of the iodide 2, 4,or 5 (2.0 mmol) and olefin 1 (1 mmol) in EtOH-H2O (1:1, 10 mL) in the dark and open to air. After complete addition, the brown mixture was allowed to stir for 1 h at r.t. DBU (457 mg, 3 mmol) was added at 0 C, and the mixture was stirred at r.t. overnight. After addition of a sat. aq solution of NH4Cl (50 mL), the mixture was extracted with Et2O (20 and 10 mL), and the organic phases were washed with brine (10 mL). The combined organic layers were dried over Na2SO4 and concentrated. The crude product was purified by column chromatography. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
95.7% | With calcium fluoride; In pentan-3-one; at 100℃; for 2.5h; | Add 0.95 Kg of <strong>[624-75-9]iodoacetonitrile</strong> and 500 g of anhydrous calcium fluoride to a 3-liter reaction flask at room temperature, add 600 ml of 3-pentanone, turn on the electric stirrer and start Heating, the temperature rose to 100 C, reaction 2.5h.2. After the above reaction, cool to 52 C, install the distillation unit, raise the temperature to 110 C, distillation, and the liquid bottle is cooled with dry ice until no liquid flows out. The product yield was 95.7% and the purity reached 96.6%.3. The above product was placed in a 500 ml single-necked flask and distilled at 120 C under normal pressure to collect 78-80 C fractions. The vial was cooled with an acetone bath to give a mixture of fluoroacetonitrile having a purity of 99.8% |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
81% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-methyl-N-benzylamines (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 10 - 1: 20) to yield corresponding alpha-aminonitriles 2422 (81%), 2522 (77%) and 26 (70%). 24 (liq): 1H NMR 2.42 (s, 3H), 3.43 (s, 2H), 3.59 (s, 2H), 7.30-7.33 (m, 5H). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
77% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-methyl-N-benzylamines (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-iodoacetonitrile (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 10 - 1: 20) to yield corresponding alpha-aminonitriles 2422 (81%), 2522 (77%) and 26 (70%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
70% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-methyl-N-benzylamines (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 10 - 1: 20) to yield corresponding alpha-aminonitriles 2422 (81%), 2522 (77%) and 26 (70%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
91% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). 11a (liq): 1H NMR 0.12 (s, 9H), 2.18 (s, 2H), 3.40 (s, 2H), 3.62 (s, 2H), 7.27-7.34 (m, 5H). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
74% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
78% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
85% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
75% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
75% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
74% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
75% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
78% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
78% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
66% | With potassium carbonate; In acetonitrile; at 20℃; for 20h; | General procedure: Individual solutions of secondary N-alpha-trimethylsilylmethyl-N-benzylamines19 (5mmol) in acetonitrile (100mL) containing K2CO3 (10mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (4.5mmol) were stirred for 12hat room temperature and concentrated in vacuo to give residues that were partitioned between water and CH2Cl2. The CH2Cl2 layers were dried and concentrated in vacuo to afford residues that were subjected to silica gel column chromatography (EtOAc/hexane=1: 15 - 1: 30) to yield corresponding alpha-aminonitiriles 11a18 (91%), 11b (74%), 11c (78%), 11d (85%), 11e (75%), 11f (75%), 11g (74%), 11h (75%), 11i (78%), 11j (78%) and 11k (66%). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
64% | With N-ethyl-N,N-diisopropylamine; In methanol; at 65℃; for 4h;Inert atmosphere; | A mixture of (2S,2'S)-3,3'-((((2,2'-dimethyl-[1,1'-biphenyl]-3,3'- diyl)bis(oxy))bis(propane-3,1-diyl))bis(azanediyl))bis(propane-1,2-diol) (Example 2079, 20 mg, 0.042 mmol) and 2-<strong>[624-75-9]iodoacetonitrile</strong> (20 mul, 0.276 mmol) in methanol (1 mL) and N,N-diisopropylethylamine (50 muL, 0.286 mmol) was heated at 65 C for 4 h. The crude material was purified via preparative LC/MS with the following conditions: Column: XBridge C18, 19 x 200 mm, 5-mum particles;Mobile Phase A: 5:95 acetonitrile: water with 10-mM ammonium acetate; Mobile Phase B: 95:5 acetonitrile: water with 10-mM ammonium acetate; Gradient: 25-65% B over 15 minutes, then a 5-minute hold at 100% B; Flow: 20 mL/min to give the pure title compound: (16.1 mg, 64%). LC/MS Condition E: ret time 1.65 min; m/e = 555 (M+H)+. LC/MS Condition F: ret time 1.45 min; m/e = 555 (M+H)+. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
92% | In dichloromethane; at 20℃; for 24h;Schlenk technique; Inert atmosphere; | General procedure: A Schlenk flask was charged with 2 (100.0 mg, 0.142mmol) andICH3 (100.8 mg, 44.2 mL, 0.710mmol), CH2Cl2 (20mL) was added.The resulting solution was stirred at room temperature for 24 h,then the solvent was reduced to 2mL under vacuum. To the residue,20mL of n-pentane was added. The yellow precipitate thusformed was filtered, washed with 2 10mL of cold n-pentane anddried under vacuum to give the product [Cp(dppe)RuN3(CH3)C2HCO2Et][I] (3a) (104.6mg, 0.124mmol) in 87% yield. Spectroscopicdata for 3a are as follows: 1H NMR (CDCl3): delta 7.95 (s, 1H,CH), 7.46-7.04 (m, 20H, Ph), 4.73 (Cp), 4.29 (q, 2H, OCH2,JHH = 7.1 Hz), 3.27 (s, 3H, NCH3), 2.95, 2.65 (2m, 2H, PCH2CH2P),1.36 (t, 3H, CH3, JHH= 7.1 Hz). 31P NMR (CDCl3): delta 84.53.13C NMR(CDCl3): delta 156.2 (CO2), 145.1 (CH), 139.0-127.8 (Ph and CCO2), 81.8(Cp), 62.7 (OCH2), 37.6 (NCH3), 28.9 (t, PCH2, JC-P = 22.7 Hz), 14.3(CH3). MS (m/z, Ru102): 720.2 (M+-I), 565.1 (MItriazolatoring). Anal. Calcd. for C37H38N3P2O2RuI: C, 52.49; H, 4.52; N, 4.96.Found: C, 53.78; H, 4.86; N, 4.69. Complex [Cp(dppe)RuN3(CH2Ph)C2HCO2Et][Br] (3b) (116.6mg, 0.121mmol, 85% yield from100.1mg of 2), [Cp(dppe)RuN3(CH2C6F5)C2HCO2Et][Br] (3c)(106.9 mg, 0.111mmol, 78% yield from 100.0 mg of 2), [Cp(dppe)RuN3(CH2CN)C2HCO2Et][I] (3d) (114.1mg, 0.131mmol, 92% yieldfrom 100.2mg of 2), [Cp(dppe)RuN3(CH2CO2CH3)C2HCO2Et)][Br](3e) (111.0 mg, 0.130mmol, 91% yield from 100.2 mg of 2) wereprepared using the similar procedure as that of 3a. Spectroscopicdata for 3b are as follows: 1H NMR (CDCl3): delta 8.30 (s, 1H, CH),7.52e6.41 (m, 25H, Ph), 4.86 (s, 2H, NCH2), 4.79 (Cp), 4.33 (q, 2H,OCH2, JHH = 7.1 Hz), 2.80, 2.60 (2m, 2H, PCH2CH2P), 1.40 (t, 3H,CH3, JHH 7.1 Hz). 31P NMR (CDCl3): delta 85.80.13C NMR (CDCl3):delta 156.5 (CO2), 145.8 (CH), 139.3-127.8 (Ph and CCO2), 82.2 (Cp),62.8 (OCH2), 53.6 (NCH2), 28.7 (t, PCH2, JC-P = 22.5 Hz), 13.9 (CH3).MS (m/z, Ru102): 796.1 (M+-Br), 565.1(MBrCH2PhN3C2HCO2Et). Anal. Calcd. for C43H42N3P2O2RuBr:C, 58.98; H, 4.83; N, 4.80. Found: C, 59.35; H, 4.98; N, 4.65.Spectroscopic data for 3c are as follows: 1HNMR (CDCl3): delta 8.43 (s,1H, CH), 7.36-7.06 (m, 20H, Ph), 5.01 (s, 2H, NCH2), 4.76 (Cp), 4.43(q, 2H, OCH2, JHH = 7.1 Hz), 2.77, 2.72 (2 m, 2H, PCH2CH2P), 1.48 (t,3H, CH3, JHH = 7.1 Hz). 31P NMR (CDCl3): delta 86.12.13C NMR (CDCl3):delta 156.4 (CO2), 145.4 (CH), 139.9-128.2 (Ph and CCO2), 82.4 (Cp),63.2 (OCH2), 39.6 (NCH2), 28.8 (t, PCH2, JC-P = 22.5 Hz), 13.7 (CH3).MS (m/z, Ru102): 886.1 (M+-Br), 565.1(MBrCH2C6F5N3C2HCO2Et). Anal. Calcd. forC43H37N3P2RuF5Br: C, 53.48; H, 3.86; N, 4.35. Found: C, 54.72; H,4.01; N, 4.13. Spectroscopic data for 3d are as follows: 1H NMR(CDCl3): delta 7.88 (s, 1H, CH), 7.56-7.21 (m, 20H, Ph), 5.05 (s, 2H,NCH2), 4.79 (Cp), 4.34 (q, 2H, OCH2, JHH = 7.1 Hz), 3.01, 2.60 (2m,2H, PCH2CH2P), 1.38 (t, 3H, CH3, JHH = 7.1 Hz). 31P NMR (CDCl3):delta 84.67.13C NMR (CDCl3): delta 155.7 (CO2), 145.8 (CH), 139.1-127.7 (Phand CCO2), 111.3 (CN), 82.0 (Cp), 63.6 (OCH2), 39.2 (NCH2), 29.0 (t,PCH2, JC-P = 22.7 Hz), 14.2 (CH3). MS (m/z, Ru102): 745.2 (M+-I),565.1 (MItriazolato ring). Anal. Calcd. for C38H37N4P2O2RuI: C,52.36; H, 4.28; N, 6.43. Found: C, 51.98; H, 4.53; N, 6.21. Spectroscopicdata for 3e are as follows: 1H NMR (CDCl3): delta 7.86 (s, 1H,CH), 7.39-7.10 (m, 20H, Ph), 4.66 (Cp), 4.42 (s, 2H, NCH2), 4.17 (q,2H, OCH2, JHH = 7.1 Hz), 3.48 (s, 3H, OCH3), 2.84, 2.59 (2m, 2H,PCH2CH2P), 1.23 (t, 3H, CH3, JHH = 7.1 Hz). 31P NMR (CDCl3):delta 84.26.13C NMR (CDCl3): delta 164.7, 156.1 (CO2), 145.0 (CH),139.1-127.7 (Ph), 137.4 (CCO2), 81.9 (Cp), 65.7 (NCH2), 62.8 (OCH2),52.8 (OCH3), 28.7 (t, PCH2, JC-P = 22.6 Hz), 15.1 (CH3). MS (m/z,Ru102): 778.1 (M+-Br), 565.0 (MBrCH2CO2CH3N3C2HCO2Et).Anal. Calcd. for C39H40N3P2O2RuBr: C, 54.62; H, 4.70; N, 4.90.Found: C, 54.93; H, 4.95; N, 4.74. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
91% | In diethyl ether; at 20℃;Cooling; | General procedure: Starting amine (5.0 mmol) was dissolved in dry diethyl ether (17 mL) and <strong>[624-75-9]iodoacetonitrile</strong> (5.5 mmol, 919 mg) was added dropwise under cooling. The mixture was stirred at room temperature for 3-14 days, then precipitate was filtered off, washed with diethyl ether and dried in vacuo to give the corresponding quaternary ammonium salt 2. If the solid precipitate was not formed after 7 days, the mixture was evaporated under reduced pressure. The crude product was washed with diethyl ether (3 × 15mL) and dried in vacuo. The quaternary ammonium salt 2 was used in the next step without additional purification. The corresponding quaternary ammonium salt (3.0 mmol) was heated at 150 C on oil bath in freshly distilled DMF (7 mL) in a 25 mL round-bottom flask fitted with reflux condenser and CaCl2-tube for 15 minutes. The mixture was cooled to room temperature, diluted with H2O (20 mL) and extracted with PhMe (2 × 15mL). The organic phase then was washed with H2O (2 × 15mL) and brine (15 mL), dried over Na2SO4 and evaporated in vacuo to give the desired aminoacetonitrile 4. The latter, if necessary, was purified by column chromatography (it is convenient to visualize aminoacetonitriles on TLC-plate by treating with the diluted solution of ninhydrin in EtOH). Note, that aminoacetonitriles are moisture sensitive. Products after column chromatography were additionally dried by the addition of dry PhMe (5 mL) and evaporation in vacuo. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
86% | In diethyl ether; at 20℃;Cooling; | General procedure: Starting amine (5.0 mmol) was dissolved in dry diethyl ether (17 mL) and <strong>[624-75-9]iodoacetonitrile</strong> (5.5 mmol, 919 mg) was added dropwise under cooling. The mixture was stirred at room temperature for 3-14 days, then precipitate was filtered off, washed with diethyl ether and dried in vacuo to give the corresponding quaternary ammonium salt 2. If the solid precipitate was not formed after 7 days, the mixture was evaporated under reduced pressure. The crude product was washed with diethyl ether (3 × 15mL) and dried in vacuo. The quaternary ammonium salt 2 was used in the next step without additional purification. The corresponding quaternary ammonium salt (3.0 mmol) was heated at 150 C on oil bath in freshly distilled DMF (7 mL) in a 25 mL round-bottom flask fitted with reflux condenser and CaCl2-tube for 15 minutes. The mixture was cooled to room temperature, diluted with H2O (20 mL) and extracted with PhMe (2 × 15mL). The organic phase then was washed with H2O (2 × 15mL) and brine (15 mL), dried over Na2SO4 and evaporated in vacuo to give the desired aminoacetonitrile 4. The latter, if necessary, was purified by column chromatography (it is convenient to visualize aminoacetonitriles on TLC-plate by treating with the diluted solution of ninhydrin in EtOH). Note, that aminoacetonitriles are moisture sensitive. Products after column chromatography were additionally dried by the addition of dry PhMe (5 mL) and evaporation in vacuo. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
94% | In diethyl ether; at 20℃;Cooling; | General procedure: Starting amine (5.0 mmol) was dissolved in dry diethyl ether (17 mL) and <strong>[624-75-9]iodoacetonitrile</strong> (5.5 mmol, 919 mg) was added dropwise under cooling. The mixture was stirred at room temperature for 3-14 days, then precipitate was filtered off, washed with diethyl ether and dried in vacuo to give the corresponding quaternary ammonium salt 2. If the solid precipitate was not formed after 7 days, the mixture was evaporated under reduced pressure. The crude product was washed with diethyl ether (3 × 15mL) and dried in vacuo. The quaternary ammonium salt 2 was used in the next step without additional purification. The corresponding quaternary ammonium salt (3.0 mmol) was heated at 150 C on oil bath in freshly distilled DMF (7 mL) in a 25 mL round-bottom flask fitted with reflux condenser and CaCl2-tube for 15 minutes. The mixture was cooled to room temperature, diluted with H2O (20 mL) and extracted with PhMe (2 × 15mL). The organic phase then was washed with H2O (2 × 15mL) and brine (15 mL), dried over Na2SO4 and evaporated in vacuo to give the desired aminoacetonitrile 4. The latter, if necessary, was purified by column chromatography (it is convenient to visualize aminoacetonitriles on TLC-plate by treating with the diluted solution of ninhydrin in EtOH). Note, that aminoacetonitriles are moisture sensitive. Products after column chromatography were additionally dried by the addition of dry PhMe (5 mL) and evaporation in vacuo. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
94% | In diethyl ether; at 20℃;Cooling; | General procedure: Starting amine (5.0 mmol) was dissolved in dry diethyl ether (17 mL) and <strong>[624-75-9]iodoacetonitrile</strong> (5.5 mmol, 919 mg) was added dropwise under cooling. The mixture was stirred at room temperature for 3-14 days, then precipitate was filtered off, washed with diethyl ether and dried in vacuo to give the corresponding quaternary ammonium salt 2. If the solid precipitate was not formed after 7 days, the mixture was evaporated under reduced pressure. The crude product was washed with diethyl ether (3 × 15mL) and dried in vacuo. The quaternary ammonium salt 2 was used in the next step without additional purification. The corresponding quaternary ammonium salt (3.0 mmol) was heated at 150 C on oil bath in freshly distilled DMF (7 mL) in a 25 mL round-bottom flask fitted with reflux condenser and CaCl2-tube for 15 minutes. The mixture was cooled to room temperature, diluted with H2O (20 mL) and extracted with PhMe (2 × 15mL). The organic phase then was washed with H2O (2 × 15mL) and brine (15 mL), dried over Na2SO4 and evaporated in vacuo to give the desired aminoacetonitrile 4. The latter, if necessary, was purified by column chromatography (it is convenient to visualize aminoacetonitriles on TLC-plate by treating with the diluted solution of ninhydrin in EtOH). Note, that aminoacetonitriles are moisture sensitive. Products after column chromatography were additionally dried by the addition of dry PhMe (5 mL) and evaporation in vacuo. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
87% | In diethyl ether; at 20℃;Cooling; | General procedure: Starting amine (5.0 mmol) was dissolved in dry diethyl ether (17 mL) and <strong>[624-75-9]iodoacetonitrile</strong> (5.5 mmol, 919 mg) was added dropwise under cooling. The mixture was stirred at room temperature for 3-14 days, then precipitate was filtered off, washed with diethyl ether and dried in vacuo to give the corresponding quaternary ammonium salt 2. If the solid precipitate was not formed after 7 days, the mixture was evaporated under reduced pressure. The crude product was washed with diethyl ether (3 × 15mL) and dried in vacuo. The quaternary ammonium salt 2 was used in the next step without additional purification. The corresponding quaternary ammonium salt (3.0 mmol) was heated at 150 C on oil bath in freshly distilled DMF (7 mL) in a 25 mL round-bottom flask fitted with reflux condenser and CaCl2-tube for 15 minutes. The mixture was cooled to room temperature, diluted with H2O (20 mL) and extracted with PhMe (2 × 15mL). The organic phase then was washed with H2O (2 × 15mL) and brine (15 mL), dried over Na2SO4 and evaporated in vacuo to give the desired aminoacetonitrile 4. The latter, if necessary, was purified by column chromatography (it is convenient to visualize aminoacetonitriles on TLC-plate by treating with the diluted solution of ninhydrin in EtOH). Note, that aminoacetonitriles are moisture sensitive. Products after column chromatography were additionally dried by the addition of dry PhMe (5 mL) and evaporation in vacuo. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
94% | In diethyl ether; at 20℃;Cooling; | General procedure: Starting amine (5.0 mmol) was dissolved in dry diethyl ether (17 mL) and <strong>[624-75-9]iodoacetonitrile</strong> (5.5 mmol, 919 mg) was added dropwise under cooling. The mixture was stirred at room temperature for 3-14 days, then precipitate was filtered off, washed with diethyl ether and dried in vacuo to give the corresponding quaternary ammonium salt 2. If the solid precipitate was not formed after 7 days, the mixture was evaporated under reduced pressure. The crude product was washed with diethyl ether (3 × 15mL) and dried in vacuo. The quaternary ammonium salt 2 was used in the next step without additional purification. The corresponding quaternary ammonium salt (3.0 mmol) was heated at 150 C on oil bath in freshly distilled DMF (7 mL) in a 25 mL round-bottom flask fitted with reflux condenser and CaCl2-tube for 15 minutes. The mixture was cooled to room temperature, diluted with H2O (20 mL) and extracted with PhMe (2 × 15mL). The organic phase then was washed with H2O (2 × 15mL) and brine (15 mL), dried over Na2SO4 and evaporated in vacuo to give the desired aminoacetonitrile 4. The latter, if necessary, was purified by column chromatography (it is convenient to visualize aminoacetonitriles on TLC-plate by treating with the diluted solution of ninhydrin in EtOH). Note, that aminoacetonitriles are moisture sensitive. Products after column chromatography were additionally dried by the addition of dry PhMe (5 mL) and evaporation in vacuo. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
96% | In diethyl ether; at 20℃;Cooling; | General procedure: Starting amine (5.0 mmol) was dissolved in dry diethyl ether (17 mL) and <strong>[624-75-9]iodoacetonitrile</strong> (5.5 mmol, 919 mg) was added dropwise under cooling. The mixture was stirred at room temperature for 3-14 days, then precipitate was filtered off, washed with diethyl ether and dried in vacuo to give the corresponding quaternary ammonium salt 2. If the solid precipitate was not formed after 7 days, the mixture was evaporated under reduced pressure. The crude product was washed with diethyl ether (3 × 15mL) and dried in vacuo. The quaternary ammonium salt 2 was used in the next step without additional purification. The corresponding quaternary ammonium salt (3.0 mmol) was heated at 150 C on oil bath in freshly distilled DMF (7 mL) in a 25 mL round-bottom flask fitted with reflux condenser and CaCl2-tube for 15 minutes. The mixture was cooled to room temperature, diluted with H2O (20 mL) and extracted with PhMe (2 × 15mL). The organic phase then was washed with H2O (2 × 15mL) and brine (15 mL), dried over Na2SO4 and evaporated in vacuo to give the desired aminoacetonitrile 4. The latter, if necessary, was purified by column chromatography (it is convenient to visualize aminoacetonitriles on TLC-plate by treating with the diluted solution of ninhydrin in EtOH). Note, that aminoacetonitriles are moisture sensitive. Products after column chromatography were additionally dried by the addition of dry PhMe (5 mL) and evaporation in vacuo. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
90% | benzoic acid (0.25 mmol, 30.6 mg), PhCH2CH2CH(Bpin) 2 (0.375 mmol, 139.8 mg) was added to a sealed tube.The reaction tube was replaced with a nitrogen atmosphere, and then 2 mL of tetrahydrofuran was added.The reaction tube was placed at -30 C under a nitrogen atmosphere.Add methyl lithium (0.625mmol, 1.6mol/L in Et2O), stir for 5min, then reverseIt should be placed in a pot at 100 C for 8 h. Subsequently, p-methoxybenzyl chloride (0.5 mmol, 78.3 mg) was added to the reaction tube under a nitrogen atmosphere, and the reaction was continued at 100 C for 6 h. At the end of the reaction, water and ethyl acetate were added for extraction, and the product was obtained by column chromatography to give a yield of 90%. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
80% | 1-Methylimidazole and 12 <strong>[624-75-9]iodoacetonitrile</strong> in 13 ethanol was refluxed and stirred at 50C for 12h, the mole ratio was 1:1. A faint yellow powder forms in the reaction. After evaporation of the solvent, the crude product was washed with ethyl acetate and diethyl ether three times, respectively, and dried under vacuum at 50C for 24h to provide pure [MCNIm][I]. Anion exchange with LiTFSI afforded the liquid-state [MCNIm][TFSI] in high yield over 80%. The chemical structure was further confirmed by 1H NMR (DMSO-d6, 400MHz): 9.26 (s, 1H), 7.90 (t, 1H), 7.81 (t, 1H), 5.60 (s, 2H), 3.89 (s, 3H). Using a similar procedure, [RCNIm][I] and [RCNIm][TFSI] from 14 1-ethylimidazole and 1-buthylimidazole can also be obtained and purified. 1HNMR (DMSO-d6, 400MHz) for [ECNIm][I]: 9.34 (s, 1H), 7.92 (t, 2H), 5.59 (s, 2H), 4.26 (m, 2H), 1.43 (s, 3H). 1HNMR (DMSO-d6, 400MHz) for [BCNIm][I]: 9.34 (s, 1H), 7.93 (t, 1H), 7.91 (t, 1H), 5.59 (s, 2H), 4.23 (m, 2H), 1.78 (m, 2H), 1.26 (m, 2H), 0.91 (t, 3H). |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
88% | With triethylamine; In N,N-dimethyl-formamide; at 0℃; for 12h; | To a stirred solution of (S)-2-((tert-butoxycarbonyl)amino)-3-(3,5-difluoro-4-hydroxyphenyl)propanoic acid (39) (740 mg, 2.33 mmol) in DMF (7.5 mL) at 0C, triethylamine (0.49 mL, 3.50 mmol) and <strong>[624-75-9]iodoacetonitrile</strong> (0.25 mL, 3.50 mmol) was added dropwise and continued stirring at room temperature for 12h. The reaction mixture was quenched with water (20 mL) and extracted with EtOAc (3 x 20 mL). The combined organic layer was washed with water (30 mL) and brine solution (30 mL), dried over Na2SO4, filtered and concentrated under reduced pressure to get the crude compound. The crude compound was first purified by ISCO combi-flash chromatogram (using 12 gm red-sep silica column, eluted with 35-45% ethylacetate in pet-ether) to afford cyanomethyl (S)-2-((tert-butoxycarbonyl)amino)-3-(3,5-difluoro-4-hydroxyphenyl)propanoate (1s) (700 mg, 1.96 mmol, 84% yield) as an off-white solid. 1H NMR (400 MHz, DMSO-d6): delta 9.97 (s, 1H), 7.42 (d, J = 7.6 Hz, 1H), 6.92 (d, J = 8.4 Hz, 2H), 5.00 (s, 2H), 4.30-4.20 (m, 1H), 2.97-2.90 (m, 1H), 2.82-2.75 (m, 1H), 1.33 (m, 9H). 19F NMR (376 MHz, DMSO-d6): delta -128.18, 132.91 (d). MS (ESI): 355.0 as [M-1] in -Ve mode. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
90% | With triethylamine; In N,N-dimethyl-formamide; at 20℃; for 12h;Cooling with ice; | To a ice cooled solution of <strong>[126727-04-6](((9H-fluoren-9-yl)methoxy)carbonyl)phenylalanine</strong> (18) (0.5 g, 1.290 mmol) and TEA (0.181 mL, 1.290 mmol) in DMF (5 mL) was added 2-iodoacetonitrile (0.093 mL, 1.290 mmol) drop-wise and the resulting solution was stirred at room temperature for 12 h. The reaction mixture was diluted with water (30 mL) and extracted with ethyl acetate (2 x 25 mL). The combined organic layer was washed with water (50 mL), saturated brine solution (50 mL), dried over anhydrous sodium sulphate, filtered and concentrated under reduced pressure to afford brown gummy liquid crude product. The crude compound was purified by silica-gel flash column chromatography (eluted with ethyl-acetate/pet-ether = 2/10) to afford Cyanomethyl (((9H-fluoren-9-yl)methoxy)carbonyl) phenylalaninate (1e) (0.5 g, 90%) as off-white solid. 1H NMR (400 MHz, DMSO-d6): delta 7.87 (d, 2H), 7.61 (d, 2H), 7.44-7.21 (m, 9H), 5.01 (s, 2H), 4.34 (d, 2H), 4.30 (t, 2H), 4.25 (t, 1H), 3.06-2.89 (m, 2H). 13C NMR (100 MHz, DMSO-d6): delta 171.4, 156.3,144.1, 141.1, 137.4, 129.6, 128.7, 128.1, 127.5, 127.0, 125.6, 120.5, 116.1, 66.1, 55.6, 49.9, 47.0, 36.5. MS (ESI): 444.2 as [M+H2O] in +Ve mode. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
88% | With triethylamine; In N,N-dimethyl-formamide; at 20℃; for 12h;Cooling with ice; | To a ice cooled stirred solution of ((benzyloxy)carbonyl)-phenylalanine (19) (0.5 g, 1.670 mmol) and TEA (0.233 mL, 1.670 mmol) in DMF (5 mL) was added 2-<strong>[624-75-9]iodoacetonitrile</strong> (0.121 mL, 1.670 mmol) drop-wise and the resulting solution was stirred at room temperature for 12 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (2 x 25 mL). Combined organic layer was washed with water (50 mL), saturated brine solution (50 mL) and dried over anhydrous sodium sulphate, filtered and concentrated under reduced pressure to afford brown gummy liquid of the crude product. The crude compound was purified by silica-gel flash column chromatography (eluted with ethyl-acetate/pet-ether = 2/10) to afford Cyanomethyl ((benzyloxy)carbonyl)phenylalaninate (1f) (0.5 g, 88%) as off-white solid. 1H NMR (300 MHz, DMSO-d6): delta 7.19-7.38 (m, 11H), 5.02 (s, 2H), 4.96 (d, 2H), 4.33-4.41 (m, 1H), 2.87-3.10 (m, 2H). 13C NMR (75 MHz, DMSO-d6): delta 171.4, 156.3, 136.4, 137.3, 137.2, 129.6, 128.8, 128.7, 128.2, 128.0, 127.1, 116.1, 65.9, 55.6, 49.9, 36.5 MS (ESI): 356.2 as [M+H2O] in +Ve mode and as 338.2 [M-1] in -Ve mode. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
88% | With isopropylmagnesium bromide; In tetrahydrofuran; at -78℃; for 2h;Inert atmosphere; | To a solution of <strong>[624-75-9]iodoacetonitrile</strong> 11 (0.16?mL, 2.10?mmol) and N-Boc piperidinone 9 (398?mg, 2.0?mmol) in THF was added i-PrMgBr (3.0?N in THF, 0.7?mL, 2.10?mmol) at -78?C under an Argon atmosphere. After being stirred for 2?h, the reaction was quenched with saturated aqueous NH4Cl solution (5.0?mL) and extracted with ethyl acetate (3?*?5.0?mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The residue was purified on silica gel (eluent: EtOAc/n-hexane?=?1:2) to give the ketoamide 12 in 88% yield as a light yellow wax. IR (film) numax: 3362, 2977, 2921, 2260, 1729, 1698, 1519, 1454, 1392, 1366, 1273, 1251, 1170, 1058, 1009?cm-1; 1H NMR (400?MHz, CDCl3) delta 1.44 (s, 9H), 1.47-1.57 (m, 2H), 1.57-1.73 (m, 2H), 2.65 (t, J?=?7.1?Hz, 2H), 3.02-3.21 (m, 2H), 3.49 (s, 2H), 4.63 (s br, 1H); 13C NMR (100?MHz, CDCl3) delta 20.2, 28.3, 29.2, 31.9, 39.8, 41.5, 79.2, 113.7, 156.0, 197.3; HRMS calcd for [C12H20N2NaO3]+ (M + Na+): 263.1366; found: 263.1365. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
75% | General procedure: To a solution of Lawsone (174 mg, 1 mmol, 1 equiv) in DMF (20 mL) was added potassium carbonate (138 mg, 1 mmol, 1 equiv).After 20 min of stirring the misture reaction at 50 C, was added <strong>[624-75-9]iodoacetonitrile</strong> (180 mL, 2.5 mmol, 2.5 equiv). The mixture reactionwas stirred at 50 C for more 4 h. After cooling, the mixture reaction was concentrated to dryness and the residue was taken up in ethylacetate (50 mL) and washed with solution of sodium bicarbonate10% in water (3 50 mL). The organic layer was dried over MgSO4, and concentrated under reduced pressure. The crude material was purified by column chromatography on silica gel with hexane/ethylacetate (9:1) to afford the desired product as yellow solid (198 mg, 93%); |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
93% | To a solution of Lawsone (174 mg, 1 mmol, 1 equiv) in DMF (20 mL) was added potassium carbonate (138 mg, 1 mmol, 1 equiv). After 20 min of stirring the misture reaction at 50 C, was added <strong>[624-75-9]iodoacetonitrile</strong> (180 mL, 2.5 mmol, 2.5 equiv). The mixture reactionwas stirred at 50 C for more 4 h. After cooling, the mixture reaction was concentrated to dryness and the residue was taken up in ethylacetate (50 mL) and washed with solution of sodium bicarbonate10% in water (3 Chi 50 mL). The organic layer was dried over MgSO4, and concentrated under reduced pressure. The crude material was purified by column chromatography on silica gel with hexane/ethylacetate (9:1) to afford the desired product as yellow solid (198 mg, 93%); mp 131-133 C; 1H NMR (300 MHz, CDCl3) delta 8.19-8.04 (m,2H), 7.82-7.73 (m, 2H), 6.33 (s, 1H), 4.89 (s, 2H); 13C NMR (75 MHz, CDCl3) delta 184.0, 178.9, 157.1, 134.9, 134.0, 131.7, 130.9, 127.0, 126.6,112.8, 112.1, 53.42; HRMS (ESI) m/z: [M +H]+ calcd for C12H8NO3:214.0499; Found: 214.0511. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
85% | With copper(l) iodide; 1,10-Phenanthroline; 1,8-diazabicyclo[5.4.0]undec-7-ene; In acetonitrile; at 110℃; for 1.5h;Sealed tube; Green chemistry; | First, a stirrer was placed in a 35 mL sealed tube, and 35 muL of styrene (0.3 mmol), 1.0 ml of acetonitrile, and 44 muL of <strong>[624-75-9]iodoacetonitrile</strong> (0.60 mmol) were added thereto, and 5·4 mg of Phen was added to the mixed solution. (0·03mmol), 5.7mgCul (0.03mmol) and 91yL DBU (0.60mmol), tightly seal the nozzle with a cock, heat to 110 C, stir for 1.5 hours, after the reaction is finished, the system is cooled to room temperature, into the reaction system 2 ml of distilled water was added, and the mixture was extracted with ethyl acetate. The organic phases were combined, and the solvent of the organic phase was evaporated under reduced pressure.36.5 mg of a colorless liquid product 3a was obtained by silica gel column chromatography, yield 85%. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
52% | With C29H29F6N2O(1+)*Br(1-); sodium hydroxide; In water; chlorobenzene; at 0℃; | In a three-necked flask, add 40 g of raw material 2 and 12 g of catalyst 3 (R = 3,5-trifluoromethylbenzyl, R1 = vinyl, R2 = hydrogen) and 1.5 L of chlorobenzene, cool to 0 C with stirring, and add 300 ml 50% aqueous sodium hydroxide solution. Subsequently, 32 g of a solution of <strong>[624-75-9]iodoacetonitrile</strong> (ICH2CN) in 500 ml of chlorobenzene was added. After the addition, the solution was stirred at 0 C until the TLC detection reaction was completed. 40 g of crude product, chiral purity (HPLC) = 72: 28, yield 80%. The crude product was recrystallized from 300 ml of ethyl acetate to obtain 25.0 g of white solid 4 with a yield of 52%, a specific rotation of + 102 (c = 1, chloroform), and a chiral HPLC ?99.92%. |
Tags: 624-75-9 synthesis path| 624-75-9 SDS| 624-75-9 COA| 624-75-9 purity| 624-75-9 application| 624-75-9 NMR| 624-75-9 COA| 624-75-9 structure
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H261 | In contact with water releases flammable gas |
H270 | May cause or intensify fire; oxidizer |
H271 | May cause fire or explosion; strong oxidizer |
H272 | May intensify fire; oxidizer |
H280 | Contains gas under pressure; may explode if heated |
H281 | Contains refrigerated gas; may cause cryogenic burns or injury |
H290 | May be corrosive to metals |
Health hazards | |
Code | Phrase |
H300 | Fatal if swallowed |
H301 | Toxic if swallowed |
H302 | Harmful if swallowed |
H303 | May be harmful if swallowed |
H304 | May be fatal if swallowed and enters airways |
H305 | May be harmful if swallowed and enters airways |
H310 | Fatal in contact with skin |
H311 | Toxic in contact with skin |
H312 | Harmful in contact with skin |
H313 | May be harmful in contact with skin |
H314 | Causes severe skin burns and eye damage |
H315 | Causes skin irritation |
H316 | Causes mild skin irritation |
H317 | May cause an allergic skin reaction |
H318 | Causes serious eye damage |
H319 | Causes serious eye irritation |
H320 | Causes eye irritation |
H330 | Fatal if inhaled |
H331 | Toxic if inhaled |
H332 | Harmful if inhaled |
H333 | May be harmful if inhaled |
H334 | May cause allergy or asthma symptoms or breathing difficulties if inhaled |
H335 | May cause respiratory irritation |
H336 | May cause drowsiness or dizziness |
H340 | May cause genetic defects |
H341 | Suspected of causing genetic defects |
H350 | May cause cancer |
H351 | Suspected of causing cancer |
H360 | May damage fertility or the unborn child |
H361 | Suspected of damaging fertility or the unborn child |
H361d | Suspected of damaging the unborn child |
H362 | May cause harm to breast-fed children |
H370 | Causes damage to organs |
H371 | May cause damage to organs |
H372 | Causes damage to organs through prolonged or repeated exposure |
H373 | May cause damage to organs through prolonged or repeated exposure |
Environmental hazards | |
Code | Phrase |
H400 | Very toxic to aquatic life |
H401 | Toxic to aquatic life |
H402 | Harmful to aquatic life |
H410 | Very toxic to aquatic life with long-lasting effects |
H411 | Toxic to aquatic life with long-lasting effects |
H412 | Harmful to aquatic life with long-lasting effects |
H413 | May cause long-lasting harmful effects to aquatic life |
H420 | Harms public health and the environment by destroying ozone in the upper atmosphere |
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