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Chemical Structure| 6358-69-6 Chemical Structure| 6358-69-6
Chemical Structure| 6358-69-6

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Synonyms: HPTS; Solvent Green 7; NSC 97285

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Pandey, Mayank ; Acharya, Vidhyalakshmi ; Shin, Ji-Yeon ; Seo, Sang-Woo ;

Abstract: The controlled uptake and release of biochemical molecules at material interfaces is critical for applications in drug delivery, biosensing, and neurostimulation. While molecularly imprinted polymers (MIPs) offer selective molecular recognition, real-time visualization of electrically controlled molecular trafficking remains underexplored. This study investigates a voltage-gated molecular interface using molecularly imprinted polypyrrole (MIP-PPy) films, designed to enable reversible uptake and release of target molecules with direct optical monitoring. Fluorescein sodium salt, a model for anionic neurotransmitters, was incorporated during electrochemical polymerization to create specific recognition sites within the polymer matrix. We employed real-time fluorescence microscopy to directly track molecule transport dynamics in response to applied voltages, utilizing a custom 3D-printed flow cell for simultaneous electrical control and in situ imaging. The MIP-PPy films demonstrated self-regenerating uptake behavior from the surrounding solution, overcoming the limitations of unidirectional release in conventional microfluidic systems. This bidirectional control introduces new opportunities for reservoir-free, adaptive chemical modulation. Biocompatibility assessments using SH-SY5Y neuroblastoma and Huh7 hepatoma cells demonstrate high levels of viability and adhesion, confirming the films’ suitability for biological integration. Overall, this study provides a visual and quantitative platform for investigating electrically induced molecular transport, which will assist in the development of closed-loop drug delivery and neurostimulation systems inspired by synaptic signaling.

Keywords: Molecule imprinted polymer ; Polypyrrole ; Targeted drug delivery ; Controlled uptake and release ; Real-time monitoring

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Larbi, Peter A ;

Abstract: Fluorescent tracer dyes are popularly used in agricultural spray application studies to evaluate on-target canopy deposition and off-target drift, and the methods/instruments used for evalu ating dye deposit must assure reliability of the data generated. A laboratory setup was con figured to adapt a submersible fluorometer for controlled and reliable measurement of pyranine dye fluorescence in liquid samples. The system setup was blanked using a clean sam ple of de-ionized water and calibrated using standard dye solutions of known concentration (100 to 1,000,000 parts per trillion [ppt]) made from two dyes of different levels of purity (pyranine 98 % and pyranine 85 %). Pyranine 85 % fluorescence readings were consistently lower with reduction factor averaging ∼0.62 times that of pyranine 98 % within a range from 500to1,000,000 ppt. The results were verified using a standard laboratory fluorometer. Spray deposition assessment of string, plastic card, cotton ribbon, and artificial foliage samplers was accomplished using the configured fluorometer system setup. The results showed no signifi cant difference among dye purity levels (P=0.430), no statistically significant interaction be tween dye purity and concentration (P=0.484), and no statistically significant interaction between dye purity and sampler type (P=0.173). Consequently, the configured setup can pro duce measurements with similar quality as the standard laboratory fluorometer and both dyes tested may be equally used for spray deposition and drift assessments, or for similar applications.

Keywords: calibration ; deposition ; detection ; linearity ; standard solution

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Product Details of Pyranine

CAS No. :6358-69-6
Formula : C16H7Na3O10S3
M.W : 524.39
SMILES Code : O=S(C1=C(C2=C34)C=CC4=C(O)C=C(S(=O)([O-])=O)C3=CC=C2C(S(=O)([O-])=O)=C1)([O-])=O.[Na+].[Na+].[Na+]
Synonyms :
HPTS; Solvent Green 7; NSC 97285
MDL No. :MFCD00037575
InChI Key :KXXXUIKPSVVSAW-UHFFFAOYSA-K
Pubchem ID :61388

Safety of Pyranine

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Application In Synthesis of Pyranine

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Upstream synthesis route of [ 6358-69-6 ]

[ 6358-69-6 ] Synthesis Path-Upstream   1~2

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  • [ 59572-10-0 ]
  • [ 6358-69-6 ]
YieldReaction ConditionsOperation in experiment
245 g With sodium hydroxide In water for 5 h; In 2000 ml three-necked flask into 1200 grams of concentrated sulfuric acid, stirring into the 120 grams of flowers, a little warming to promoteAfter all the dissolved, slowly warming to 90 ° C for 1 hour, three bottles with a water bath to cool below 40 ° C, drop the liquid sulfur black 480 grams, 3 hours drop finished. And then to maintain the temperature of about 100 ° C reaction for 5 hours, down to room temperature, the three bottles of material into the 720 grams of ice water, into a sticky material, filter dry filter cake, get pyrene tetrasulfonic acid tetrasodium salt. And then the pyrene tetrasulfonic acid tetrasodium salt dissolved in 1000 ml of water into the 2000 ml three-necked flask, stirring with a plate of sodium hydroxide to neutral, then add 300 grams of flaky sodium hydroxide, slowly warming To micro-boiling state for 5 hours. After cooling, the first with hydrochloric acid and PH value of 7 or so, then add 100 grams of sodium chloride stirring dissolved, slowly precipitated light yellow solid, filtered and dried to get 8-hydroxy-1,3, 6-pyrene three Sodium sulfonate salt about 245 grams.
References: [1] Patent: CN105272886, 2016, A, . Location in patent: Paragraph 0013; 0026; 0027.
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  • [ 6358-69-6 ]
References: [1] Journal of the American Chemical Society, 1984, vol. 106, # 26, p. 8086 - 8093.
 

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