Products Cited in Publications Worldwide Identification of key metabolites in fermented quinoa and their α-glucosidase inhibitory mechanisms using widely targeted metabolomics and molecular simulation

Identification of key metabolites in fermented quinoa and their α-glucosidase inhibitory mechanisms using widely targeted metabolomics and molecular simulation

Zhao, Li; Liao, Juan; Zhao, Haijiao; Feng, Cuiping

DOI: PMID:

Abstract

This study systematically analyzed metabolite changes in quinoa after lactic acid bacteria (LAB) fermentation and the hypoglycemic mechanism of key metabolites inhibiting α-glucosidase. Mixed LAB fermentation significantly altered the metabolic profile of quinoa, and a total of 627 differential metabolites were identified, among which 471 were significantly up-regulated. After fermentation, the levels of flavonoids, phenolic acids, and amino acid derivatives increased significantly, and five new flavonoid metabolites were detected. The polyphenol extract from fermented quinoa (FQP) significantly inhibited α-glucosidase activity, with an IC₅₀ value of 0.17 mg/mL. Mechanistic studies indicated that FQP exerted mixed-type reversible inhibition, forming hydrogen bonds, van der Waals forces, and hydrophobic interactions with amino acid residues in the active center of α-glucosidase. These interactions induced conformational changes in the enzyme, thereby reducing its catalytic efficiency. This research provides a theoretical basis for the functional processing of quinoa and the development of novel hypoglycemic functional factors.

Keywords

Fermented quinoa ; Widely targeted metabolomics ; α-Glucosidase inhibition ; Hypoglycemic mechanisms ; Polyphenols

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Quercetin

A1453902117-39-595%

Quercetin

43.0/100g

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Norartocarpetin

A1669343520-30-998%

Norartocarpetin

1389.0/50mg

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Rhamnocitrin

A239568569-92-695%

Rhamnocitrin

146.0/5mg

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Gallic Acid

A311911149-91-798%

Gallic Acid

23.0/25g

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Acarbose

A35760356180-94-098%

Acarbose

10.0/1mg

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Morin

A361330480-16-098%

Morin

37.0/5g

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(+)-Catechin

A499582154-23-497%

(+)-Catechin

634.0/25g

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