[ CAS No. 22235-25-2 ] {[proInfo.proName]}

Name: 22235-25-2|Methyl 3-amino-5-(trifluoromethyl)benzoate|97%
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SKU: A322851
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Quality Control of [ 22235-25-2 ]

COA NMR CNMR HPLC LC-MS

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SDS Specification

Alternatived Products of [ 22235-25-2 ]

Product Details of [ 22235-25-2 ]

CAS No. :	22235-25-2	MDL No. :	MFCD00625827
Formula :	C₉H₈F₃NO₂	Boiling Point :	-
Linear Structure Formula :	-	InChI Key :	UOVPHHSFTNRQHJ-UHFFFAOYSA-N
M.W :	219.16	Pubchem ID :	4026781
Synonyms :

Calculated chemistry of [ 22235-25-2 ]

Physicochemical Properties

Num. heavy atoms :	15
Num. arom. heavy atoms :	6
Fraction Csp3 :	0.22
Num. rotatable bonds :	3
Num. H-bond acceptors :	5.0
Num. H-bond donors :	1.0
Molar Refractivity :	47.13
TPSA :	52.32 Å²

Pharmacokinetics

GI absorption :	High
BBB permeant :	Yes
P-gp substrate :	No
CYP1A2 inhibitor :	Yes
CYP2C19 inhibitor :	No
CYP2C9 inhibitor :	No
CYP2D6 inhibitor :	No
CYP3A4 inhibitor :	No
Log Kp (skin permeation) :	-6.23 cm/s

Lipophilicity

Log Po/w (iLOGP) :	1.87
Log Po/w (XLOGP3) :	1.98
Log Po/w (WLOGP) :	3.23
Log Po/w (MLOGP) :	2.36
Log Po/w (SILICOS-IT) :	2.05
Consensus Log Po/w :	2.3

Druglikeness

Lipinski :	0.0
Ghose :	None
Veber :	0.0
Egan :	0.0
Muegge :	0.0
Bioavailability Score :	0.55

Water Solubility

Log S (ESOL) :	-2.54
Solubility :	0.626 mg/ml ; 0.00286 mol/l
Class :	Soluble
Log S (Ali) :	-2.7
Solubility :	0.433 mg/ml ; 0.00197 mol/l
Class :	Soluble
Log S (SILICOS-IT) :	-3.01
Solubility :	0.215 mg/ml ; 0.000979 mol/l
Class :	Soluble

Medicinal Chemistry

PAINS :	0.0 alert
Brenk :	1.0 alert
Leadlikeness :	1.0
Synthetic accessibility :	1.74

Safety of [ 22235-25-2 ]

Signal Word:	Warning	Class:	N/A
Precautionary Statements:	P261-P305+P351+P338	UN#:	N/A
Hazard Statements:	H302-H315-H319-H335	Packing Group:	N/A
GHS Pictogram:

Application In Synthesis of [ 22235-25-2 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

Upstream synthesis route of [ 22235-25-2 ]
Downstream synthetic route of [ 22235-25-2 ]

[ 22235-25-2 ] Synthesis Path-Upstream 1~5

1
[ 22227-63-0 ]
[ 22235-25-2 ]

Yield	Reaction Conditions	Operation in experiment
98%	With tin(II) chloride dihdyrate; water In methanol at 70℃; for 2 h;	To a mixture of methyl 3-nitro-5- (trifluoromethyl)benzoate (350 mg, 1.40 mmol) and tin(II) chloride dihydrate (1.58 g, 7.02 mmol) in methanol (20 mL) was added water (1 mL) and the resulting mixture was stirred at 70 °C for 2 h. After cooling to room temperature, the reaction mixture was concentrated and was quenched by the addition of saturated sodium bicarbonate solution. The mixture was extracted with ethyl acetate (3X). The combined organic portion was dried over magnesium sulfate, was filtered and was concentrated to give methyl 3-amino-5- (trifluoromethyl)benzoate (300 mg, 98percent yield) as a colorless solid. ^XH NMR (400 MHz,CDCI₃): δ 7.65 (s, 1H), 7.48 (s, 1H), 7.05 (s, 1H), 3.99 (br s, 2H), 3.91 (s, 3H).
96%	With hydrogen In methanol at 20℃; for 3 h;	To a solution of methyl 3-nitro-5-(trifluoromethyl) benzoate (1.50 g, 6.02 mmol) in methanol (20 . mL) was added palladium carbon (50percent water-containing product,15 mg) , and the mixture was stirred under a hydrogen atmosphere at room temperature for 3 hr. The reaction mixture was filtered through celite. The filtrate was concentrated under reduced pressure and dried to give the title compound(1.27 g, 96percent) as a white solid.¹H-NMR (DMSO-d₆, 300 MHz) δ 3.85 (3H, s) , 5.94 (2H, s) , 7.08 (IH, s), 7.26 (IH, s), 7.42 (IH, s) .
95%	With hydrogen In ethanol at 20℃; for 1 h;	Reference Example 28 Methyl 3-amino-5-(trifluoromethyl)benzoate A solution of methyl 3-nitro-5-(trifluoromethyl)benzoate (Reference Example 27, 24.6 g, 98.9 mmol) in ethanol (1500 mL) was sparged in a Parr bottle with nitrogen for 10 min. After this time, 10 wt percent Pd/C (5.0 g, 5.0 mmol) was added and the reaction mixture was subjected to 40 psi of hydrogen on Parr shaker at room temperature for 1 h. After this time, the reaction mixture was filtered through a pad of celite and concentrated under reduced pressure to provide Methyl 3-amino-5-(trifluoromethyl)benzoate (20.6 g, 95percent) as a light purple oil. ¹H NMR (500 MHz, CDCl₃) δ ppm 3.92 (s, 3H), 4.02 (br s, 2H), 7.05 (s, 1H), 7.48 (s, 1H), 7.64 (s, 1H).

86%	With hydrogen In methanol	Methyl 3-amino-5-(trifluoromethyl)benzoate. Methyl 3-nitro-5-(trifluoromethyl)benzoate (9.0 g, 36.1 mmol) in methanol (30 mL) was flushed with nitrogen, and treated with palladium (10percent on charcoal, 0.90 g). The flask was flushed with hydrogen and allowed to stir under an atmosphere of hydrogen overnight. The reaction was flushed with nitrogen, filtered through celite, and concentrated. Flash chromatography on silica gel (30percent ethyl acetate/hexanes) afforded 6.8 g (86percent). ¹H-NMR (CDCl_3,500 MHz) δ 7.64 (s, H), 7.49 (s, 1H), 7.05 (s, 1H), 3.91 (s, 3H). Mass spec.: 220.05 (MH)⁺.
74%	Stage #1: With tin(ll) chloride In ethanol at 60℃; for 4 h;	A solution of 3-nitro-5-trifluoromethyl-benzoic acid methyl ester (8.6 mmol, 2.15 g) and tin chloride dihydrate (25.8 mmol, 5.82 in ethanol (40 ml) is allowed to warm to 60 ⁰C and stir for 4 hours. The mixture is cooled to room temperature, then concentrated under reduced pressure. The obtained residue is neutralized with saturated aq. NaHCO₃. The mixture is filtered and concentrated under reduced pressure. The obtained residue is purified by silica gel column chromatography (eluent: hexane / EtOAc) to give 3-amino-5- trifluoromethyl-benzoic acid methyl ester (74percent, 1.4 g); ESI-MS m/z: 220 [M+1]\\ Retention time 1.81 min (condition A).
0.85 g	With 10% palladium on activated charcoal; hydrogen In methanol at 20℃; for 3 h;	(Reference Example 28) Synthesis of methyl 3-amino-5-(trifluoromethyl)benzoate: A solution of 3-nitro-5-(trifluoromethyl)benzoic acid (1.0 g, 4.2 mmol) and p-toluenesulfonic acid (0.05 g) in methanol was stirred while heating under reflux for eight hours. The obtained solution was returned to room temperature and then concentrated under vacuum. The obtained residue was dissolved in methanol, palladium (5.0percent by weight) on carbon (containing 50percent water by weight, 0.2 g) was added thereto, and the obtained solution was stirred at room temperature for three hours under hydrogen atmosphere. The reaction mixture was filtered through Celite® and the filtrate was concentrated under vacuum. The obtained crude product was purified by silica gel column chromatography (hexane:ethyl acetate = 2:1, Rf = 0.47) to obtain the title compound (0.85 g, 91percent) (hereinafter referred to as the compound of Reference Example 28) as an oily substance. MS(ESI) [M + H]⁺: 220.

Reference： [1] Patent: WO2012/37132, 2012, A1, . Location in patent: Page/Page column 47
[2] Patent: WO2007/4749, 2007, A1, . Location in patent: Page/Page column 125
[3] Patent: US2012/71489, 2012, A1, . Location in patent: Page/Page column 59
[4] Patent: US2007/249607, 2007, A1, . Location in patent: Page/Page column 72
[5] Patent: WO2008/9435, 2008, A1, . Location in patent: Page/Page column 159; 160
[6] Bioorganic and Medicinal Chemistry, 2006, vol. 14, # 12, p. 4158 - 4181
[7] Journal of Medicinal Chemistry, 2014, vol. 57, # 23, p. 9971 - 9982
[8] Patent: EP3345892, 2018, A1, . Location in patent: Paragraph 0326; 0327

2
[ 328-80-3 ]
[ 22235-25-2 ]

Reference： [1] Bioorganic and Medicinal Chemistry, 2006, vol. 14, # 12, p. 4158 - 4181
[2] Patent: WO2012/37132, 2012, A1,
[3] Patent: US2012/71489, 2012, A1,
[4] Journal of Medicinal Chemistry, 2014, vol. 57, # 23, p. 9971 - 9982
[5] Chemistry - A European Journal, 2017, vol. 23, # 52, p. 12758 - 12762
[6] Patent: EP3345892, 2018, A1,

3
[ 67-56-1 ]
[ 328-80-3 ]
[ 22235-25-2 ]

Yield	Reaction Conditions	Operation in experiment
0.85 g	Stage #1: for 15 h; Reflux Stage #2: With 10% palladium on activated carbon; Degussa type; hydrogen In methanol; water for 1 h;	To a solution of 3-nitro-5-(trifluoromethyl)benzoic acid (1.0 g, 4.25 mmol) in methanol, a catalyst amount of p-toluenesulfonic acid was added, and the obtained solution was heated under reflux for 15 hours. The obtained reaction liquid was returned to room temperature and then concentrated to the half of the original volume. Palladium (10percent by weight) on carbon (containing 50percent water by weight, 0.062 g) was added to the reaction liquid, and the obtained solution was stirred for one hour under hydrogen atmosphere. The reaction liquid was filtered through Celite®, the filtrate was concentrated under vacuum, and the obtained crude product was purified by silica gel column chromatography (eluent; hexane:ethyl acetate = 90:10 → 60:40) to obtain the title compound (0.85 g). MS(ESI) [M + H]⁺: 221.

Reference： [1] Patent: EP3345893, 2018, A1, . Location in patent: Paragraph 0385

4
[ 67-56-1 ]
[ 328-68-7 ]
[ 22235-25-2 ]

Reference： [1] Chemistry - A European Journal, 2017, vol. 23, # 52, p. 12758 - 12762

5
[ 454-92-2 ]
[ 22235-25-2 ]

Reference： [1] Chemistry - A European Journal, 2017, vol. 23, # 52, p. 12758 - 12762

[ 22235-25-2 ] Synthesis Path-Downstream 1~64

1
[ 463-71-8 ]
[ 22235-25-2 ]
[ 955947-12-3 ]

Reference： [1]Bioorganic and Medicinal Chemistry Letters,2007,vol. 17,p. 28 - 33
[2]Journal of the American Chemical Society,2016,vol. 138,p. 13525 - 13528

2
[ 3592-25-4 ]
[ 22235-25-2 ]
[ 334792-87-9 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181
[2]Journal of Medicinal Chemistry,2014,vol. 57,p. 9971 - 9982

3
[ 22227-63-0 ]
[ 22235-25-2 ]

Yield	Reaction Conditions	Operation in experiment
98%	With tin(II) chloride dihdyrate; water; In methanol; at 70℃; for 2.0h;	To a mixture of methyl 3-nitro-5- (trifluoromethyl)benzoate (350 mg, 1.40 mmol) and tin(II) chloride dihydrate (1.58 g, 7.02 mmol) in methanol (20 mL) was added water (1 mL) and the resulting mixture was stirred at 70 °C for 2 h. After cooling to room temperature, the reaction mixture was concentrated and was quenched by the addition of saturated sodium bicarbonate solution. The mixture was extracted with ethyl acetate (3X). The combined organic portion was dried over magnesium sulfate, was filtered and was concentrated to give methyl 3-amino-5- (trifluoromethyl)benzoate (300 mg, 98percent yield) as a colorless solid. XH NMR (400 MHz,CDCI3): delta 7.65 (s, 1H), 7.48 (s, 1H), 7.05 (s, 1H), 3.99 (br s, 2H), 3.91 (s, 3H).
96%	With hydrogen;palladium on activated charcoal; In methanol; at 20℃; for 3.0h;	To a solution of methyl 3-nitro-5-(trifluoromethyl) benzoate (1.50 g, 6.02 mmol) in methanol (20 . mL) was added palladium carbon (50percent water-containing product,15 mg) , and the mixture was stirred under a hydrogen atmosphere at room temperature for 3 hr. The reaction mixture was filtered through celite. The filtrate was concentrated under reduced pressure and dried to give the title compound(1.27 g, 96percent) as a white solid.1H-NMR (DMSO-d6, 300 MHz) delta 3.85 (3H, s) , 5.94 (2H, s) , 7.08 (IH, s), 7.26 (IH, s), 7.42 (IH, s) .
95%	With hydrogen;palladium 10% on activated carbon; In ethanol; at 20℃; under 2068.65 Torr; for 1.0h;	Reference Example 28Methyl 3-amino-5-(trifluoromethyl)benzoateA solution of methyl 3-nitro-5-(trifluoromethyl)benzoate (Reference Example 27, 24.6 g, 98.9 mmol) in ethanol (1500 mL) was sparged in a Parr bottle with nitrogen for 10 min.After this time, 10 wt percent Pd/C (5.0 g, 5.0 mmol) was added and the reaction mixture was subjected to 40 psi of hydrogen on Parr shaker at room temperature for 1 h.After this time, the reaction mixture was filtered through a pad of celite and concentrated under reduced pressure to provide Methyl 3-amino-5-(trifluoromethyl)benzoate (20.6 g, 95percent) as a light purple oil.1H NMR (500 MHz, CDCl3) delta ppm 3.92 (s, 3H), 4.02 (br s, 2H), 7.05 (s, 1H), 7.48 (s, 1H), 7.64 (s, 1H).

86%	With hydrogen;palladium 10% on activated carbon; In methanol;	Methyl 3-amino-5-(trifluoromethyl)benzoate. Methyl 3-nitro-5-(trifluoromethyl)benzoate (9.0 g, 36.1 mmol) in methanol (30 mL) was flushed with nitrogen, and treated with palladium (10percent on charcoal, 0.90 g). The flask was flushed with hydrogen and allowed to stir under an atmosphere of hydrogen overnight. The reaction was flushed with nitrogen, filtered through celite, and concentrated. Flash chromatography on silica gel (30percent ethyl acetate/hexanes) afforded 6.8 g (86percent). 1H-NMR (CDCl3, 500 MHz) delta 7.64 (s, H), 7.49 (s, 1H), 7.05 (s, 1H), 3.91 (s, 3H). Mass spec.: 220.05 (MH)+.
74%		A solution of 3-nitro-5-trifluoromethyl-benzoic acid methyl ester (8.6 mmol, 2.15 g) and tin chloride dihydrate (25.8 mmol, 5.82 in ethanol (40 ml) is allowed to warm to 60 0C and stir for 4 hours. The mixture is cooled to room temperature, then concentrated under reduced pressure. The obtained residue is neutralized with saturated aq. NaHCO3. The mixture is filtered and concentrated under reduced pressure. The obtained residue is purified by silica gel column chromatography (eluent: hexane / EtOAc) to give 3-amino-5- trifluoromethyl-benzoic acid methyl ester (74percent, 1.4 g); ESI-MS m/z: 220 [M+1]\\ Retention time 1.81 min (condition A).
0.85 g	With palladium on activated charcoal; hydrogen; In methanol; at 20℃; for 3.0h;	(Reference Example 28) Synthesis of methyl 3-amino-5-(trifluoromethyl)benzoate: A solution of 3-nitro-5-(trifluoromethyl)benzoic acid (1.0 g, 4.2 mmol) and p-toluenesulfonic acid (0.05 g) in methanol was stirred while heating under reflux for eight hours. The obtained solution was returned to room temperature and then concentrated under vacuum. The obtained residue was dissolved in methanol, palladium (5.0percent by weight) on carbon (containing 50percent water by weight, 0.2 g) was added thereto, and the obtained solution was stirred at room temperature for three hours under hydrogen atmosphere. The reaction mixture was filtered through Celite® and the filtrate was concentrated under vacuum. The obtained crude product was purified by silica gel column chromatography (hexane:ethyl acetate = 2:1, Rf = 0.47) to obtain the title compound (0.85 g, 91percent) (hereinafter referred to as the compound of Reference Example 28) as an oily substance. MS(ESI) [M + H]+: 220.

Reference： [1]Patent: WO2012/37132,2012,A1 .Location in patent: Page/Page column 47
[2]Patent: WO2007/4749,2007,A1 .Location in patent: Page/Page column 125
[3]Patent: US2012/71489,2012,A1 .Location in patent: Page/Page column 59
[4]Patent: US2007/249607,2007,A1 .Location in patent: Page/Page column 72
[5]Patent: WO2008/9435,2008,A1 .Location in patent: Page/Page column 159; 160
[6]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181
[7]Journal of Medicinal Chemistry,2014,vol. 57,p. 9971 - 9982
[8]Patent: EP3345892,2018,A1 .Location in patent: Paragraph 0326; 0327

4
[ 1445-45-0 ]
[ 22235-25-2 ]
methyl 3-(5-methyl-1H-tetrazol-1-yl)-5-(trifluoromethyl)benzoate [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
58%		Methyl 3-(5-methyl-1H-tetrazol-1-yl)-5-(trifluoromethyl)benzoate. Trimethylorthoacetate (0.41 mL, 3.4 mmol) in acetic acid (3 mL) was added dropwise to a solution of <strong>[22235-25-2]methyl 3-amino-5-(trifluoromethyl)benzoate</strong> (0.5 g, 2.28 mmol) in acetic acid (5 mL) at 75 C. After stirring for 45 min at 75 C., the reaction was treated with sodium azide (0.21 g, 3.4 mmol) carefully in portions over 15 min and stirring continued for 3 h. After cooling to room temperature, the reaction was concentrated and the residue dissolved in ethyl acetate. This was washed with water (2×), 1 N hydrochloric acid (2×), brine (2×), dried over sodium sulfate, and concentrated. Flash chromatography on silica gel (20% ethyl acetate/hexanes) gave 0.38 g (58%). LC/MS (HPLC method 3): tR=2.17 min, 287.12(MH)-.

Reference： [1]Patent: US2007/249607,2007,A1 .Location in patent: Page/Page column 72

5
[ 22235-25-2 ]
[ 537039-44-4 ]

Yield	Reaction Conditions	Operation in experiment
100%		To a suspension of lithium aluminum hydride (4 mmol, 152 mg) in THF (5 ml_) which is cooled to 0 0C is dropwise added a solution of <strong>[22235-25-2]3-amino-5-trifluoromethyl-benzoic acid methyl ester</strong> (2 mmol, 438 mg) in THF (1 mL) under nitrogen. The solution is allowed to warm to rt and stirred for 2 hours. To the solution is added diethyl ether (6 mL), then quenched with sodium sulfate decahydrate and brine. After decantation, the solution is dried over Na2SO4, filtrated, and concentrated under reduced pressure to give (3-amino-5- trifluoromethyl-phenyl)-methanol (quantitative yield), which is used in the next step without further purification); ESI-MS m/z: 192 [M+1]+, Retention time 1.30 min (condition A).
92%	With lithium aluminium tetrahydride; In tetrahydrofuran; at 20℃; for 1h;Cooling with ice;	(Reference Example 30) Synthesis of (3-amino-5-(trifluoromethyl)phenyl)methanol: To a solution of the compound of Reference Example 28 (0.4 g, 1.8 mmol) in tetrahydrofuran, lithium aluminium hydride (0.22 g, 5.7 mmol) was added portionwise under cooling on ice. The obtained reaction mixture was returned to room temperature and then stirred for one hour. After adding water to the reaction mixture to stop the reaction, the obtained solution was extracted with ethyl acetate and sequentially washed with a saturated aqueous solution of ammonium chloride and with saturated brine. The organic layer was dried over anhydrous sodium sulfate and then concentrated under vacuum, and the obtained crude product was purified by silica gel column chromatography (hexane:ethyl acetate = 1:1, Rf = 0.32) to obtain the title compound (0.32 g, 92%) (hereinafter referred to as the compound of Reference Example 30) as a white solid. MS(ESI) [M + H]+: 192.
0.32 g	With lithium aluminium tetrahydride; In tetrahydrofuran;Cooling with ice;	To a solution of <strong>[22235-25-2]methyl 3-amino-5-(trifluoromethyl)benzoate</strong> (0.4 g, 1.825 mmol) in THF, lithium aluminium hydride (0.215 g, 5.66 mmol) was slowly added under cooling on ice. The ice bath was removed, and the obtained solution was stirred overnight. Water was slowly added to the reaction solution, and the obtained solution was extracted with ethyl acetate. The organic layer was sequentially washed with a saturated aqueous solution of ammonium chloride and with a saturated aqueous solution of sodium chloride. The obtained organic layer was dried over anhydrous sodium sulfate and then concentrated under vacuum, and the obtained crude product was purified by silica gel column chromatography (eluent; hexane:ethyl acetate = 90:10 ? 60:40) to obtain the title compound (0.32 g). MS(ESI) [M + H]+: 371.

Reference： [1]Patent: WO2008/9435,2008,A1 .Location in patent: Page/Page column 159; 160
[2]Patent: EP3345892,2018,A1 .Location in patent: Paragraph 0330; 0331
[3]Patent: EP3345893,2018,A1 .Location in patent: Paragraph 0387

6
[ 22235-25-2 ]
3-(2-carbamoyl-2-cyano-thioacetylamino)-5-trifluoromethyl-benzoic acid methyl ester [ No CAS ]

Reference： [1]Bioorganic and Medicinal Chemistry Letters,2007,vol. 17,p. 28 - 33

7
[ 22235-25-2 ]
3-(4-cyano-3-hydroxy-isothiazol-5-ylamino)-5-trifluoromethyl-benzoic acid methyl ester [ No CAS ]

Reference： [1]Bioorganic and Medicinal Chemistry Letters,2007,vol. 17,p. 28 - 33

8
[ 328-80-3 ]
[ 22235-25-2 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181
[2]Patent: WO2012/37132,2012,A1
[3]Patent: US2012/71489,2012,A1
[4]Journal of Medicinal Chemistry,2014,vol. 57,p. 9971 - 9982
[5]Chemistry - A European Journal,2017,vol. 23,p. 12758 - 12762
[6]Patent: EP3345892,2018,A1

9
[ 22235-25-2 ]
[ 334792-91-5 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181
[2]Journal of Medicinal Chemistry,2014,vol. 57,p. 9971 - 9982

10
[ 22235-25-2 ]
[ 334792-88-0 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181

11
[ 22235-25-2 ]
[ 334792-90-4 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181

12
[ 22235-25-2 ]
[ 334792-89-1 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181

13
[ 22235-25-2 ]
[ 334792-92-6 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181
[2]Journal of Medicinal Chemistry,2014,vol. 57,p. 9971 - 9982

14
[ 22235-25-2 ]
[ 334792-93-7 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181
[2]Journal of Medicinal Chemistry,2014,vol. 57,p. 9971 - 9982

15
[ 22235-25-2 ]
[ 334792-95-9 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181

16
[ 22235-25-2 ]
[ 334792-94-8 ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181

17
[ 22235-25-2 ]
WA₄₃₆ [ No CAS ]

Reference： [1]Bioorganic and Medicinal Chemistry,2006,vol. 14,p. 4158 - 4181
[2]Journal of Medicinal Chemistry,2014,vol. 57,p. 9971 - 9982

18
[ 22235-25-2 ]
[ 75-03-6 ]
[ 677704-77-7 ]

Yield	Reaction Conditions	Operation in experiment
	With potassium carbonate; In DMF (N,N-dimethyl-formamide); at 65℃; for 14h;	A mixture of [3-AMINO-5-(TRIFLUOROMETHYL)-BENZOIC] acid methyl ester (J. Med. Chem. (1969) 12,299-303 ; 4.23 g, 19.3 [MMOL),] potassium carbonate (8.0 g, 57.9 [MMOL)] and iodoethane (3.12 mL, 38.6 [MMOL)] in 20 mL [N, N-DIMETHYLFORMAMIDE] is stirred at [65C] for 14 hours in a tightly closed vessel. After cooling, the reaction mixture is filtered and the filtrate evaporated to dryness under reduced pressure. The residue is treated with water and extracted three times with ethyl acetate. The combined extracts are dried [(NA2SO4)] and the solvent is evaporated off under reduced pressure. The resulting residue is purified by column chromatography on silica gel, eluent hexane/methylene chloride (1: 1). ['H-NMR] (400 MHz, [DMSO-D6,] 8) : 1.18 (t, 3H); 3.10 (m, 2H); 3.85 (s, 3H); 6.46 (t, 1H) ; 7.02 [(BR. 1 H) ; 7.29 (BR. S, 1 H) ; 7.37 (BR. , 1 H).]

Reference： [1]Patent: WO2004/29038,2004,A1 .Location in patent: Page 43

19
[ 632625-42-4 ]
[ 22235-25-2 ]
3-{2-[5-chloro-2-(benzyloxy)phenyl]-5-methyl-pyrrol-1-yl}-5-trifluoromethylbenzoic acid methyl ester [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	toluene-4-sulfonic acid; In 1-methyl-pyrrolidin-2-one; at 150℃; for 0.166667h;Microwave;	[1- [5-CHLORO-2-BENZYLOXY)-PHENYL]-PENTANE-1,] 4-dione (104mg), 3-amino-5-trifluoromethyl- benzoic acid methyl ester (85mg) and [PTSA] (cat) were heated in NMP (1.5mL) at [150C] in a microwave for 10 minutes. The mixture was diluted with [ET20] and washed sequentially with 2M HCI and saturated [NAHCO3,] dried [(NA2SO4),] filtered and concentrated. The residue was purified on silica gel with iso-hexane containing EtOAc (5%) to give the title compound (70mg) LC/MS t=4.02 min, [MH+] 500,502.

Reference： [1]Patent: WO2003/101959,2003,A1 .Location in patent: Page 238-239

20
[ 632627-07-7 ]
[ 22235-25-2 ]
3-{2-[5-bromo-2-(4-fluorobenzyloxy)phenyl]-5-methyl-pyrrol-1-yl}-5-trifluoromethylbenzoic acid methyl ester [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
34%	toluene-4-sulfonic acid; In 1-methyl-pyrrolidin-2-one; at 180℃; for 0.166667h;Microwave irradiation;	[1- [5-BROMO-2- (4-FLUORO-BENZYLOXY)-PHENYL]-PENTANE-1,] 4-dione (0.10g, 0. [26MOL),] 3- amino-5-trifluoromethyl-benzoic acid methyl ester (0.055g, 0. [26MMOL)] and [P-TSA] (cat. ) in NMP (1 ml) were heated in a sealed vessel at [180C] for 10 minutes using a microwave. Upon cooling the reaction was diluted with EtOAc [(25MOI)] and washed with dil. NaHCO3, dil. [HCI] and brine, dried [(MGS04),] filtered and concentrated. The residue was purified by column chromatography using a Si SPE cartridge (5g) eluting in [1-20%] EtOAc/i-hex. to give the title compound (50mg, 34%). LC/MS t=4.39 min [[MH+]] 562/564.

Reference： [1]Patent: WO2003/101959,2003,A1 .Location in patent: Page 163

21
[ 632627-17-9 ]
[ 22235-25-2 ]
3-{2-[5-fluoro-2-(4-fluorobenzyloxy)phenyl]-5-methyl-pyrrol-1-yl}-5-trifluoromethyl-benzoic acid methyl ester [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
47%	toluene-4-sulfonic acid; In acetonitrile; at 160℃; for 0.166667h;Microwave irradiation;	[1- [5-FLUORO-2- (4-FLUORO-BENZYLOXY)-PHENYL]-PENTANE-1,] 4-dione (0.15g, 0. [47MMOL),] 3-amino- [5-TRIFLUOROMETHYL-BENZOIC] acid methyl ester (0.099g, 0. [47MMOL)] and [P-TSA] (cat. ) in CH3CN [(1ML)] were heated in a sealed vessel at [160C] for 10 minutes using a microwave. Upon cooling the reaction was diluted with [CH2CI2] (5ml) and shaken with dil. [HCI] [(1] ml). The organics were separated using a phase separator column with a NaS04 cartridge attached and concentrated. The residue was purified by column chromatography using [BIOTAGE 25M] cartridge, [WITH ISO-HEXANE/ETOAC] (5-50%) as eluant, to give the title compound [(110MG,] 47%). LC/MS t=4.16 min [MH+] 502.

Reference： [1]Patent: WO2003/101959,2003,A1 .Location in patent: Page 172-173

22
[ 632627-48-6 ]
[ 22235-25-2 ]
3-{2-[5-chloro-2-(4-bromo-2-fluorobenzyloxy)phenyl]-5-methyl-pyrrol-1-yl}-5-trifluoromethylbenzoic acid methyl ester [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
36%	With toluene-4-sulfonic acid; In acetonitrile; at 160℃; for 0.166667h;Microwave irradiation;	[1- [5-CHLORO-2- (4-BROMO-2-FLUORO-BENZYLOXY)-PHENYL]-PENTANE-1,] 4-dione (150mg, 0. [363MMOL),] <strong>[22235-25-2]3-amino-5-trifluoromethylbenzoic acid methyl ester</strong> (80mg, 0. [363MOL)] and [PTSA] (5mg) were heated in [ACETONITRILE (2ML)] at [160C] for 10 minutes in the microwave. Upon cooling, the mixture was diluted with DCM and washed with 2M HCI, dried (Na2SO4), filtered and concentrated. The residue was purified by chromatography, on silica, with cyclohexane containing a gradient of EtOAc (0-5%) to give the title compound (78mg, 36%). [1H] NMR [(400MHZ,] [CDCI3)] 2.18 (3H, s), 3.90 (3H, s), 4.70 (2H, s), 6.16 (1 H, d, J=3. 0Hz), 6.30 (1H, d, J=3.3Hz), 6.60 (1H, d, J=8.8Hz), 6.85-6. 91 (1H, m), 7.10-7. 15 (1H, m), 7.21 (1H, s), 7.23 (1H, s), 7.26-7. 29 (1H, m's excess), 7.39 (1H, s), 7.84 (1H, s), 8.15 (1H, s).

Reference： [1]Patent: WO2003/101959,2003,A1 .Location in patent: Page 198

23
[ 632627-55-5 ]
[ 22235-25-2 ]
3-{2-[5-chloro-2-(2,6-difluorobenzyloxy)phenyl]-5-methyl-pyrrol-1-yl}-5-trifluoromethylbenzoic acid methyl ester [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
32%	With toluene-4-sulfonic acid; In acetonitrile; at 160℃; for 0.166667h;Microwave irradiation;	[1- [5-CHLORO-2- (2, 6-DIFLUORO-BENZYLOXY)-PHENYL]-PENTANE-1,] 4-dione (150mg, 0. [43MMOL),] 3- [AMINO-5-TRIFLUOROMETHYLBENZOIC] acid methyl ester (93mg, 0. 43mmol) and [PTSA] (5mg) were heated in acetonitrile (2ml) at [160C] for 10 minutes in the microwave. Upon cooling, the mixture was diluted with DCM and washed with 2M HCI, dried [(NA2SO4),] filtered and concentrated. The residue was purified by chromatography, on silica, with cyclohexane containing a gradient of EtOAc (0-5%) to give the title compound (73mg, [32%).] 'H NMR [(400MHZ,] [CDCI3)] 2.15 (3H, s), 3.93 (3H, s), 4.78 (2H, s), 6.09 [(1H,] d, J=3.5Hz), 6.25 (1H, d, J=3.5Hz), 6. 81 (1H, d, J=8.5Hz), 6.83-6. 91 (2H, m), 7.12-7. 18 (2H, m), 7.26- 7.34 (1H, m's excess), 7.39 (1H, s), 7.86 (1H, s), 8.15 (1H, s).

Reference： [1]Patent: WO2003/101959,2003,A1 .Location in patent: Page 206

24
[ 632627-62-4 ]
[ 22235-25-2 ]
3-{2-[5-chloro-2-(2,3-difluorobenzyloxy)phenyl]-5-methyl-pyrrol-1-yl}-5-trifluoromethylbenzoic acid methyl ester [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
48%	With toluene-4-sulfonic acid; In acetonitrile; at 160℃; for 0.166667h;Microwave irradiation;	[1- [5-CHLORO-2- (2, 3-DIFLUORO-BENZYLOXY)-PHENYL]-PENTANE-1, 4-DIONE] (150mg, 0. [43MMOL),] 3- amino-5-trifluoromethylbenzoic acid methyl ester (93mg, 0. [43MMOL)] and [PTSA] (5mg) were heated in acetonitrile (2ml) at [160C] for 10 minutes in the microwave. Upon cooling, the mixture was diluted with DCM and washed with 2M HCI, dried [(NA2SO4),] filtered and concentrated. The residue was purified by chromatography, on silica, with cyclohexane containing a gradient of EtOAc (0-5%) to give the title compound [(107MG,] 48%). 'H NMR [(400MHZ,] CDCI3) 2.17 (3H, s), 3.90 (3H, s), 4.77 (2H, s), 6.16 [(1H,] d, J=3. 0Hz), 6.31 (1H, d, J=3.5Hz), 6.62 (1H, d, J=8.8Hz), 6.71-6. 77 (1H, m), 6.96-7. 03 (1H, m), 7.06- 7.16 (2H, m), 7.25-7. 31 (1H, m's excess), 7.39 (1H, s), 7.84 (1H, s), 8.15 (1H, s)

Reference： [1]Patent: WO2003/101959,2003,A1 .Location in patent: Page 213-214

25
[ 632627-76-0 ]
[ 22235-25-2 ]
3-{2-[5-methyl-2-(4-fluorobenzyloxy)phenyl]-5-methyl-pyrrol-1-yl}-5-trifluoromethylbenzoic acid methyl ester [ No CAS ]

Yield	Reaction Conditions	Operation in experiment
	toluene-4-sulfonic acid; In 1-methyl-pyrrolidin-2-one; at 150℃; for 0.166667h;Microwave irradiation;	1- [5-Methyl-2- (4-fluoro-benzyloxy)-phenyl]-pentane-1, 4-dione [(110MG),] 3-amino-5- trifluoromethyl-benzoic acid methyl ester (96mg) and [PTSA] (cat) were heated in NMP at [150C] in a microwave for 10 minutes. The mixture was diluted with EtOAc and washed sequentially with 2M [HCI] and saturated NaHCO3, dried (Na2SO4), filtered and concentrated. The residue was purified on silica gel with iso-hexane containing EtOAc (3- 6%) to give the title compound. LC/MS t=4.28 min, [MH+] 498.

Reference： [1]Patent: WO2003/101959,2003,A1 .Location in patent: Page 231

Yield	Reaction Conditions	Operation in experiment
100%		Intermediate 47: Methyl 3-amino-5-(trifluoromethyl)benzoate Methanol (20 ml) was added to intermediate 46 (5.0 g, 20 mmol) to prepare a solution, and 3.0 g of 10% palladium-carbon was added to the solution. The mixturewas stirred in a hydrogen atmosphere at room temperature for 23.5 hr. The insolubles were filtered and were concentrated under the reduced pressure to give the title compound (4.3 g, 100%). Physicochemical properties of intermediate 47

27
[ 326-90-9 ]
[ 22235-25-2 ]
[ 1202635-15-1 ]

Reference： [1]Bioorganic and Medicinal Chemistry Letters,2009,vol. 19,p. 6725 - 6732

28
[ 22235-25-2 ]
[ 1214361-12-2 ]

Yield	Reaction Conditions	Operation in experiment
		Reference Example 29 Methyl 3-chloro-5-(trifluoromethyl)benzoate To a stirred solution of <strong>[22235-25-2]methyl 3-amino-5-(trifluoromethyl)benzoate</strong> (Reference Example 28, 2.15 g, 9.81 mmol) in acetonitrile (50.0 mL) was added tert-butyl nitrite (3.04 g, 29.48 mmol) at room temperature. After 15 min copper(II) chloride (2.38 g, 17.70 mmol) was added and the reaction mixture was stirred overnight. After this time, the mixture was diluted with ethyl acetate, washed with water, saturated ammonium hydrochloride, and saturated sodium chloride, dried (Na2SO4), filtered and concentrated under reduced pressure to provide methyl 3-chloro-5-(trifluoromethyl)benzoate (2.20 g, 94%) as a brown oil, which was used without further purification or characterization.

Reference： [1]Patent: US2012/71489,2012,A1 .Location in patent: Page/Page column 59

29
[ 22235-25-2 ]
[ 886496-87-3 ]

Reference： [1]Patent: US2012/71489,2012,A1

30
[ 22235-25-2 ]
[ 477535-43-6 ]

Reference： [1]Patent: US2012/71489,2012,A1

31
[ 22235-25-2 ]
[ 1252657-92-3 ]

Reference： [1]Patent: US2012/71489,2012,A1

32
[ 24424-99-5 ]
[ 22235-25-2 ]
[ 1395416-25-7 ]

Yield	Reaction Conditions	Operation in experiment
76%	With dmap; In dichloromethane; at 20℃; for 2h;	To a solution of <strong>[22235-25-2]3-amino-5-trifluoromethyl-benzoic acid methyl ester</strong> (2 g, 9.13 mmol, CAS RN 22235-25-2) in CH2Cl2 (30 mL) was added di-tert-butyl dicarbonate (1.99 g, 9.13 mmol, CAS RN 24424-99-5) and DMAP (1.11 g, 9.13 mmol). The reaction mixture was stirred at room temperature for 2 hours. The reaction mixture was treated with silica gel and concentrated under vacuum and purified by silica gel chromatography using a MPLC system (CombiFlash Companion, Isco Inc.) eluting with a gradient of n-heptane:EtOAc (100:0 to 70:30). Colorless solid (76%). MS (GC_MS (EI)): 319.1 (M+).
76%	With dmap; In dichloromethane; at 20℃; for 2h;	To a solution of <strong>[22235-25-2]3-amino-5-trifluoromethyl-benzoic acid methyl ester</strong> (2 g, 9.13 mmol, CAS RN 22235-25-2) in CH2C12 (30 mL) was added di-ie/ -butyl dicarbonate (1.99 g, 9.13 mmol, CAS RN 24424-99-5) and DMAP (1.11 g, 9.13 mmol). The reaction mixture was stirred at room temperature for 2 hours. The reaction mixture was treated with silica gel andconcentrated under vacuum and purified by silica gel chromatography using a MPLC system (CombiFlash Companion, Isco Inc.) eluting with a gradient of n-heptane : EtOAc (100 : 0 to 70 : 30). Colorless solid (76%). MS (GC_MS (EI)): 319.1 (M+).

Reference： [1]Patent: US2012/232051,2012,A1 .Location in patent: Page/Page column 97
[2]Patent: WO2012/117000,2012,A1 .Location in patent: Page/Page column 210

33
[ 22235-25-2 ]
(S)-methyl 2-(4-((2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethyl)carbamoyl)phenylsulfonamido)-3-(3-(4-(pyrimidin-2-ylamino)piperidin-1-yl)-5-(trifluoromethyl)benzamido)propanoate [ No CAS ]