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Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Chemical Structure| 193149-74-5 Chemical Structure| 193149-74-5

Structure of WST-8
CAS No.: 193149-74-5

Chemical Structure| 193149-74-5

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WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

Synonyms: Water-Soluble Tetrazolium 8

4.5 *For Research Use Only !

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Product Citations

Product Citations

Shin-nosuke Nishimura ; Tomoya Yoshida ; Nobuyuki Higashi ; Tomoyuki Koga ;

Abstract: The elastic modulus of hydrogels is one of the most important factors forcontrolling cell fate. In this study, hydrogels composed ofpoly(N-acryloylglycinamide) (PNAGAm) grafted with arginine (R)–glycine(G)–aspartic acid (D)–serine (S) is designed without a chemicalcrosslinker. The hydrogels are prepared by the conventional radicalcopolymerization of N-acryloylglycinamide with a polymerizable RGDSpeptide. The grafting ratio is easily controlled by adjusting the feedcomposition for polymerization. The hydrogels exhibit thermo-responsivenessof the upper critical solution temperature type based on the PNAGAm moiety.This characteristic reflects the shape fix/memory properties of hydrogelsbecause of the reversible formation of multiple hydrogen bonds. The celladhesiveness of the hydrogels drastically improves in the presence of a smallamount of the graft. The hydrogels exhibit good biocompatibility; theadhered cells proliferate on the hydrogels, and macrophages do not showactivation or inflammation. Surfaces with different elastic modulus regionsare successfully constructed on the same hydrogel by using shapefix/memory properties, allowing the regulation of cell adhesion. This hydrogelsystem offers promising opportunities for the design and application offunctional cell scaffolds.

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Product Details of WST-8

CAS No. :193149-74-5
Formula : C20H13N6NaO11S2
M.W : 600.47
SMILES Code : [O-]S(C1=CC(S([O-])(=O)=O)=CC=C1C2=NN(C(C(OC)=C3)=CC=C3[N+]([O-])=O)[N+](C(C=C4)=CC=C4[N+]([O-])=O)=N2)(=O)=O.[Na+]
Synonyms :
Water-Soluble Tetrazolium 8
MDL No. :MFCD09264687
InChI Key :VSIVTUIKYVGDCX-UHFFFAOYSA-M
Pubchem ID :9894947

Safety of WST-8

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H228-H315-H319
Precautionary Statements:P240-P210-P241-P264-P280-P302+P352-P370+P378-P337+P313-P305+P351+P338-P362+P364-P332+P313
Class:4.1
UN#:1325
Packing Group:

Isoform Comparison

Biological Activity

Description
WST-8, a water-soluble tetrazolium dye, is favored for its heightened sensitivity in assays compared to MTS-based assays.

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
Balb/c mice Colon cancer xenograft model Oral 50 mg/kg Once daily for 3 weeks To evaluate the enhancement of the antitumor effect of L-OHP by simvastatin. Results showed that simvastatin significantly enhanced the antitumor effect of L-OHP. PMC10108455

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

1.67mL

0.33mL

0.17mL

8.33mL

1.67mL

0.83mL

16.65mL

3.33mL

1.67mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2
The prepared working fluid is recommended to be prepared now and used up as soon as possible in a short period of time. The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1

References

 

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