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Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Chemical Structure| 111072-31-2 Chemical Structure| 111072-31-2

Structure of XTT sodium
CAS No.: 111072-31-2

Chemical Structure| 111072-31-2

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Product Citations

Product Citations

Tan, Junjun ; Liu, Qiong ; Liu, Zhiping ; Cao, Yanli ; Yu, Xiaomin ; Zhao, Qianjun , et al.

Abstract: Small heat shock proteins are evolutionarily conserved molecular chaperones with diverse cellular roles. However, our understanding of their functions in pathogenic microorganisms remains limited. In this study, we identified and characterized Fmp28, a novel small heat shock protein that enhances virulence in Candida albicans, and explored its regulatory mechanism. The expression of Fmp28 was significantly upregulated at both transcriptional and translational levels when C. albicans was subjected to physiological temperature (37°C), osmotic stress, or cell wall stress. Deletion of Fmp28 significantly impaired growth, adhesion, biofilm formation, invasive growth, and virulence at physiological temperature (37°C), respectively. Transcriptome analysis identified Als3 as a key downstream target of Fmp28. Notably, Als3 overexpression substantially rescued the impairments in adhesion, biofilm formation, invasion, and virulence caused by FMP28 deletion. Deletion of FMP28 also led to downregulated expression of genes in the cAMP-PKA pathway, as well as reduced intracellular and levels. By adding exogenous analogs, the adhesion and biofilm formation in the fmp28Δ/Δ mutant could be partially restored. In conclusion, the findings in this study demonstrated that Fmp28 maintained C. albicans virulence at physiological temperature by regulating Als3 expression through the cAMP-PKA signaling pathway, establishing Fmp28 as a potential therapeutic target for treating C. albicans infections.

Keywords: small heat shock protein ; Fmp28 ; Candida albicans ; Als3 ; virulence

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Product Details of XTT sodium

CAS No. :111072-31-2
Formula : C22H16N7NaO13S2
M.W : 673.52
SMILES Code : O=C(C1=NN(C2=C(OC)C=C([N+]([O-])=O)C(S(=O)([O-])=O)=C2)[N+](C3=C(OC)C=C([N+]([O-])=O)C(S(=O)([O-])=O)=C3)=N1)NC4=CC=CC=C4.[Na+]
MDL No. :MFCD00083517
InChI Key :JACYMBNQPPWQML-UHFFFAOYSA-M
Pubchem ID :14195569

Safety of XTT sodium

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H315-H319-H228
Precautionary Statements:P240-P210-P241-P264-P280-P302+P352-P370+P378-P337+P313-P305+P351+P338-P362+P364-P332+P313
Class:4.1
UN#:1325
Packing Group:

Isoform Comparison

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

1.48mL

0.30mL

0.15mL

7.42mL

1.48mL

0.74mL

14.85mL

2.97mL

1.48mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
 

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