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Chemical Structure| 581065-95-4 Chemical Structure| 581065-95-4
Chemical Structure| 581065-95-4

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Amino-PEG4-Boc is a 4-unit PEG derivative with an amino group and a Boc-protecting group. It is used in peptide conjugation, PROTAC synthesis, and bioconjugation.

4.5 *For Research Use Only !

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Product Details of Amino-PEG4-Boc

CAS No. :581065-95-4
Formula : C15H31NO6
M.W : 321.41
SMILES Code : O=C(OC(C)(C)C)CCOCCOCCOCCOCCN
MDL No. :MFCD11041116
InChI Key :PKESARRNSGIDRD-UHFFFAOYSA-N
Pubchem ID :51340928

Safety of Amino-PEG4-Boc

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Application In Synthesis of Amino-PEG4-Boc

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Downstream synthetic route of [ 581065-95-4 ]

[ 581065-95-4 ] Synthesis Path-Downstream   1~1

  • 1
  • [ 581065-95-4 ]
  • [ 72040-64-3 ]
  • C31H56N4O9S [ No CAS ]
YieldReaction ConditionsOperation in experiment
87% The commercial product 6-Biotinylamino-hexanoic Acid (Biotin-Ahx-OH, 247 mg, 0.69 mmol) was dissolved in DMF (10 ml, light suspension). The carboxylic acid of substrate was activated by HCTU (347 mg, 0.84 mmol) and DIEA (298muIota 1.7 mmol) for 10 min. at room temperature. The activated solution was then added to commercial compound tBuO-Peg3- NH2 (98.6 mg, 0.76 mmol) solution (1 ml dry DMF). Then, others DIEA (92 muIota, 0.5 mmol) was added and reaction mixture was stirred at room temperature for 2h. The organic solvent was evaporated under high vacuum and the crude product obtained was dissolved in with CH2CI2 (20 ml), washed with NaHC03 5percent, (7 ml) and with brine (7 ml), dried over anhydrous Na2S04, filtered, concentrated and purified by silica gel chromatograph (CH2CI2/MeOH 9:1 ) to give a white semi solid compound (401 mg, yield 87 percent) and was used without further purification. The structure was confirmed by 1H-NMR spectroscopy analysis (500 MHz): delta [ppm, D20 (d2)], 4.61 (dd, 1 H biot); 4.42 (dd, 1 H, biot); 3.78 (4H, t, -OCH2CH2CO-); 3.69 (12H, s, - CH2CH20-) 3.33 (1 H, Biot.), 3.21 ( t, 2H, -OCH2CH2NH2x TFA); 3.18 (t, 4H , - CH2CH2NHCO-); 3.01 (1 H, dd, biot); 2.759 (1 H, d, biot.) 2.5 (2H, t, -CH2C02NH-); 2.25 (2H, t, -CH2C02NH-); 1 .79-1 .31 (12H, m, -CH2-, biot .); and Mass spectroscopy Q-Tof (m/z) [M+H+]= 355.16; [M+Na+]= 377.16; [M+K+] =393.13.
 

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