Structure of 5131-60-2
*Storage: {[sel_prStorage]}
*Shipping: {[sel_prShipping]}
The BI-3802 was designed by Boehringer Ingelheim and could be obtained free of charge through the Boehringer Ingelheim open innovation portal opnMe.com, associated with its negative control.
4.5
*For Research Use Only !
Change View
| Size | Price | VIP Price |
DE Stock US Stock |
Asia Stock Global Stock |
In Stock |
| {[ item.pr_size ]} |
Inquiry
{[ getRatePrice(item.pr_usd, 1,1,item.pr_is_large_size_no_price, item.pr_usd) ]} {[ getRatePrice(item.pr_usd,item.pr_rate,1,item.pr_is_large_size_no_price, item.discount_usd) ]} {[ getRatePrice(item.pr_usd, 1,1,item.pr_is_large_size_no_price, item.pr_usd) ]} |
Inquiry {[ getRatePrice(item.pr_usd,item.pr_rate,item.mem_rate,item.pr_is_large_size_no_price, item.vip_usd) ]} | {[ item.p_spot_brand_remark ]} 1-2 weeks {[ item.pr_usastock ]} In Stock Inquiry - | {[ item.p_spot_brand_remark ]} 1-2 weeks {[ item.pr_chinastock ]} {[ item.pr_remark ]} In Stock Inquiry - | Login - + |
Please Login or Create an Account to: See VIP prices and availability
Asia Stock: Ship in 3-5 business days
EU Stock: ship in 0-1 business day
Global Stock: ship in 5-7 days
US Stock: ship in 0-1 business day
Global Stock: ship in 5-7 days
{[ item.p_spot_brand_remark ]}
1-2weeks
Inquiry
Inquiry
{[ getRatePrice(item.pr_usd,item.pr_rate,item.mem_rate,item.pr_is_large_size_no_price, item.vip_usd) ]}
{[ getRatePrice(item.pr_usd, 1,1,item.pr_is_large_size_no_price, item.pr_usd) ]}
{[ getRatePrice(item.pr_usd,1,item.mem_rate,item.pr_is_large_size_no_price, item.pr_usd) ]}
{[ item.p_spot_brand_remark ]}
1-2weeks
Inquiry
Inquiry
{[ getRatePrice(item.pr_usd,item.pr_rate,1,item.pr_is_large_size_no_price, item.vip_usd) ]}
{[ getRatePrice(item.pr_usd, 1,1,item.pr_is_large_size_no_price, item.pr_usd) ]}
{[ getRatePrice(item.pr_usd, 1,1,item.pr_is_large_size_no_price, item.pr_usd) ]}
In Stock
- +
Please Login or Create an Account to: See VIP prices and availability
Asia Stock: Ship in 3-5 business days
EU Stock: ship in 0-1 business day
Global Stock: ship in 5-7 days
US Stock: ship in 0-1 business day
Global Stock: ship in 5-7 days
Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
Search for reports by entering the product batch number.
Batch number can be found on the product's label following the word 'Batch'.
Systematic analysis of gut bacterial carcinogen metabolism and its functional consequences
Boyao Zhang ; George-Eugen Maftei ; Bartosz Bartmanski ; Michael Zimmermann ;
Abstract: Organic carcinogens, in particular DNA-reactive compounds, contribute to the irreversible initiation step of tumorigenesis through introduction of genomic instability. Although carcinogen bioactivation and detoxification by human enzymes has been extensively studied, carcinogen biotransformation by human-associated bacteria, the microbiota, has not yet been systematically investigated. We tested the biotransformation of 68 mutagenic carcinogens by 34 bacterial species representative for the upper and lower human gastrointestinal tract and found that the majority (41) of the tested carcinogens undergo bacterial biotransformation. To assess the functional consequences of microbial carcinogen metabolism, we developed a pipeline to couple gut bacterial carcinogen biotransformation assays with Ames mutagenicity testing and liver biotransformation experiments. This revealed a bidirectional crosstalk between gut microbiota and host carcinogen metabolism, which we validated in gnotobiotic mouse models. Overall, the systematic assessment of gut microbiota carcinogen biotransformation and its interplay with host metabolism highlights the gut microbiome as an important modulator of exposome-induced tumorigenesis.
Show More >
Purchased from AmBeed: 446-86-6 ; 121-66-4 ; 607-35-2 ; 67-20-9 ; 105650-23-5 ; 59-87-0 ; 117-39-5 ; 57-97-6 ; 5131-60-2 ; 512-56-1 ; 62-44-2 ; 6959-48-4 ; 84-65-1 ; 137-17-7 ; 117-39-5 ; 153-78-6 ; 1614-12-6 ; 298-81-7 ; 404-86-4 ; 320-67-2 ; 99-55-8 ; 94-52-0 ; 2832-40-8 ; 101-61-1 ; 103-33-3 ; 114-83-0 ; 64091-91-4 ; 53-96-3 ; 3817-11-6 ; 90-94-8 ; 613-13-8 ; 56-57-5 ; 91-64-5 ; 26148-68-5 ; 101-80-4 ; 139-65-1 ; 366-70-1 ; 389-08-2 ; 99-59-2 ; 132-32-1 ; 394-69-4 ; 3544-23-8 ; 389-08-2 ; 320-67-2 ; 82-28-0 ; 2475-45-8 ; 129-15-7
Show More >
| CAS No. : | 5131-60-2 |
| Formula : | C6H7ClN2 |
| M.W : | 142.59 |
| SMILES Code : | NC1=CC=C(Cl)C(N)=C1 |
| MDL No. : | MFCD00025284 |
| InChI Key : | ZWUBBMDHSZDNTA-UHFFFAOYSA-N |
| Pubchem ID : | 21209 |
| GHS Pictogram: |
|
| Signal Word: | Warning |
| Hazard Statements: | H315-H319-H335 |
| Precautionary Statements: | P261-P305+P351+P338 |
| Num. heavy atoms | 9 |
| Num. arom. heavy atoms | 6 |
| Fraction Csp3 | 0.0 |
| Num. rotatable bonds | 0 |
| Num. H-bond acceptors | 0.0 |
| Num. H-bond donors | 2.0 |
| Molar Refractivity | 40.26 |
| TPSA ? Topological Polar Surface Area: Calculated from |
52.04 Ų |
| Log Po/w (iLOGP)? iLOGP: in-house physics-based method implemented from |
1.17 |
| Log Po/w (XLOGP3)? XLOGP3: Atomistic and knowledge-based method calculated by |
0.85 |
| Log Po/w (WLOGP)? WLOGP: Atomistic method implemented from |
1.52 |
| Log Po/w (MLOGP)? MLOGP: Topological method implemented from |
1.41 |
| Log Po/w (SILICOS-IT)? SILICOS-IT: Hybrid fragmental/topological method calculated by |
1.07 |
| Consensus Log Po/w? Consensus Log Po/w: Average of all five predictions |
1.2 |
| Log S (ESOL):? ESOL: Topological method implemented from |
-1.75 |
| Solubility | 2.52 mg/ml ; 0.0177 mol/l |
| Class? Solubility class: Log S scale |
Very soluble |
| Log S (Ali)? Ali: Topological method implemented from |
-1.53 |
| Solubility | 4.25 mg/ml ; 0.0298 mol/l |
| Class? Solubility class: Log S scale |
Very soluble |
| Log S (SILICOS-IT)? SILICOS-IT: Fragmental method calculated by |
-2.27 |
| Solubility | 0.766 mg/ml ; 0.00537 mol/l |
| Class? Solubility class: Log S scale |
Soluble |
| GI absorption? Gatrointestinal absorption: according to the white of the BOILED-Egg |
High |
| BBB permeant? BBB permeation: according to the yolk of the BOILED-Egg |
Yes |
| P-gp substrate? P-glycoprotein substrate: SVM model built on 1033 molecules (training set) |
No |
| CYP1A2 inhibitor? Cytochrome P450 1A2 inhibitor: SVM model built on 9145 molecules (training set) |
No |
| CYP2C19 inhibitor? Cytochrome P450 2C19 inhibitor: SVM model built on 9272 molecules (training set) |
No |
| CYP2C9 inhibitor? Cytochrome P450 2C9 inhibitor: SVM model built on 5940 molecules (training set) |
No |
| CYP2D6 inhibitor? Cytochrome P450 2D6 inhibitor: SVM model built on 3664 molecules (training set) |
No |
| CYP3A4 inhibitor? Cytochrome P450 3A4 inhibitor: SVM model built on 7518 molecules (training set) |
No |
| Log Kp (skin permeation)? Skin permeation: QSPR model implemented from |
-6.57 cm/s |
| Lipinski? Lipinski (Pfizer) filter: implemented from |
0.0 |
| Ghose? Ghose filter: implemented from |
None |
| Veber? Veber (GSK) filter: implemented from |
0.0 |
| Egan? Egan (Pharmacia) filter: implemented from |
0.0 |
| Muegge? Muegge (Bayer) filter: implemented from |
1.0 |
| Bioavailability Score? Abbott Bioavailability Score: Probability of F > 10% in rat |
0.55 |
| PAINS? Pan Assay Interference Structures: implemented from |
0.0 alert |
| Brenk? Structural Alert: implemented from |
1.0 alert: heavy_metal |
| Leadlikeness? Leadlikeness: implemented from |
No; 1 violation:MW<1.0 |
| Synthetic accessibility? Synthetic accessibility score: from 1 (very easy) to 10 (very difficult) |
1.37 |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

[ 5131-60-2 ]
[ 3964-52-1 ]
[ 5131-60-2 ]
[ 138-89-6 ]
[ 5131-60-2 ]
[ 98-59-9 ]
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| 100% | With tin(II) chloride dihdyrate; In ethanol; at 20℃; | A mixture of 1-chloro-2,4-dinitro-benzene (100 mg, 0.5 mmol) and SnCl2.2H2O (1.12 g, 5 mmol) in ethanol (2.5 mL) was stirred at room temperature overnight. Water was added and then the mixture was basified to pH 7-8 with saturated NaHCO3 solution. The solution was extracted with ethyl acetate. The combined organic layers were washed with brine, dried over Na2SO4, filtered and concentrated to yield 4-chloro-benzene-1,3-diamine (D-1) (79 mg, quant.). HPLC ret. time 0.38 min, 10-99% CH3CN, 5 min run; ESI-MS 143.1 m/z (MH+). |
| With platinum on activated charcoal; In methanol; at 30℃; under 3750.38 Torr; for 1.5h;Autoclave;Catalytic behavior; | General procedure: PtL1/L2/C (25.0 mg, 1.282 mol of Pt) and the desired amountof substrate were placed in a Teflon-coated stainless steel auto-clave (80.0 mL), equipped with magnetic stirrer, temperature andpressure controller. The autoclave was then sealed and evacu-ated, followed by charging it with deaerated MeOH (10.0 mL)by suction. Afterwards, the autoclave was placed in an oil pathwhich was heated to 30.0C. Once the latter temperature wasreached, the autoclave was pressurized with hydrogen (5.0 bar) and stirred at 800 rpm. After the desired reaction time, the auto-clave was removed from the oil bath and cooled to 20.0C, followedby venting the excess hydrogen. The suspension was centrifugedand the catalytic solution separated from catalyst by decantationin air atmosphere. To the latter solutions n-dodecane (100.0 L,0.44 mmol) was added as external standard and analyzed by GC.Recycling experiments were conducted by using recovered PtL1/2,applying the above descript protocol. The Ptsurface-atom related TOF(h-1) values were calculated according to the following equation:mmol(converted substrate) × [mmol(Ptsurface) × h]-1.The mmolof Ptsurface(i.e. amount of Pt atoms exposed to the substrate)using 25.0 mg of catalyst for the hydrogenation reactions were0.7179 mol for PtL1and 0.7948 mol for PtL2and PtC,according to the percentage of Pt-atoms localized on the NPs? sur-face (i.e. 56% (PtL1) and 62% for PtL2/C), using a HRTEM-basedcalculation [35]. |
[ 5131-60-2 ]
[ 56-81-5 ]
[ 40505-09-7 ]
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| (b) Amino compounds of the formula (XV) and (XV a)1,3-Diamino-benzene, 4-chloro-1,3-diamino-benzene, 2,4-diaminotoluene, 2,6-diamino-toluene, 1,4-diamino-naphthalene, 2,7-diamino-naphthalene, 1,5-diamino-naphthalene-3,7-disulphonic acid, 1,6-diamino-naphthalene-4,8-disulphonic acid, 1,4-diamino-naphthalene-6-sulphonic acid, ... | ||
| methylamine, ...2-ethyl-n-butylenediamine, 2-hydroxy-n-propylenediamine, 1,3-diaminobenzene, 1,4-diaminobenzene, 1,3-diamino-4-chlorobenzene, 1,3-diamino-4-methylbenzene, 1,3-diamino-4-ethylbenzene, 1,3-diamino-4-methoxybenzene, ... | ||
| ...he aromatic nucleus, for example alkyl groups and halogen atoms. ...1,4-phenylenediamine2,4-tolylenediamine2,4-diaminoanisole2,4-diaminochlorobenzene2,5-diaminoanisole1,2-phenylenediamine2,6-tolylenediamine1,4-diaminonaphthalene... |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| With benzoyl chloride; triethylamine; In dichloromethane; at 0℃; for 16h; | benzoyl chloride (5.2 ml) was added to a stirred mixture of 2,4-diaminochlorobenzene (6.42 g), triethylamine (12.5 ml) and methylene chloride (100 ml) which had been cooled to 0°C. The mixture was allowed to warm to ambient temperature and was stirred for 16 hours.. The mixture was evaporated and the residue was triturated under a saturated aqueous sodium bicarbonate solution.. The resultant solid was isolated, washed in turn with water and isohexane and dried under vacuum at 55°C. There was thus obtained N-(3-amino-4-chlorophenyl)benzamide as a solid (10.38 g); NMR Spectrum: (DMSOd6) 5.32 (s, 2H), 6.9 (m, 1H), 7.1 (d, 1H), 7.37 (d, 1H), 7.52 (m, 3H), 7.9 (d, 2H), 10.05 (s, 1H). |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| With aluminum (III) chloride; In toluene; at 20℃; | 206 g of N,N'-dicyclohexylcarbodiimide and 71 g of 2,4-diaminodichlorobenzene were mixed with 500 ml of toluene, and to this was added 3 g of aluminum chloride in several portions at room temperature. Upon completion of the heat generation, to this was added 1 liter of methanol and 20 g of activated carbon, and the mixture was stirred for 1 hour while the inner temperature was kept at 50 C. The reaction solution was filtered to remove solids, about 0.3 liters of methanol was distilled off under reduced pressure, and the residue was cooled until the inner temperature reached 10 C. The precipitated crystals were filtered and dried to obtain 183 g of crystals of the guanidine compound of Specific example (2)-4. Identification of the guanidine compound of Specific Example (2) -4 was conducted using 1H-NMR (solvent: DMSO-d6, 300 MHz). Data are shown below. 1H-NMR (delta, ppm): 0.98 to 1.30 (m, 20 H), 1.56 (m, 4 H), 1.63 (m, 8 H), 1.86 (m, 8 H), 3.39 (m, 4 H), 4.74 (m, 4 H), 6.05 (d, 1 H), 6.16 (dd, 1 H), 7.06 (d, 1 H) |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| 88% | 15.0 ml of pyridine are added to a solution of 173.0 g (0.9 mol) of ETHYLBENZOYL acetate in 3.2 litres of xylene, and the mixture is heated to 130-135°C. At that temperature, 107.0 g (0.75 mol) of 4-chloro-1, 3-phenylenediamine are added in portions to the solution and the ethynol that is liberated is distilled off continuously. After 5 hours, the reddish-brown solution is cooled to 25°C and 14.3 g (75 mol) of p-toluenesulfonic acid are added thereto. The resulting green suspension is then boiled for 6 hours under reflux, the water of reaction being distilled off. The suspension, which is now yellow, is allowed to cool to room tempera- ture. Filtration is then carried out, the precipitate is washed with a total of 500 mi of ethanol in three portions, and the crude product is dried for 20 hours at 80°C under a laboratory vacuum. There are obtained 179 G (0. 66 mol, yield 88 percent of theory) of A beige-brown powder of formula which, after recrystallisation from acetic acid, is in the form of white crystals having a melting point of 327°C. |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| With triethylamine; In dichloromethane; | The N-(3-amino-4-chlorophenyl)-2-nitrobenzamide used as a starting material was prepared as follows: 2-Nitrobenzoyl chloride (4.64 ml) was added to a stirred mixture of 3-amino-4-chloroaniline (5 g), triethylamine (9.78 ml) and methylene chloride (300 ml) and the reaction mixture was stirred at ambient temperature for 16 hours. The mixture was partitioned between methylene chloride and a saturated aqueous sodium bicarbonate solution. The organic phase was evaporated and the residue was purified by column chromatography on silica to give the required starting material (3.02 g); NMR Spectrum: (DMSOd6) 5.38 (s, 2H), 6.74 (d, 1H), 7.11 (d, 1H), 7.27 (s, 1H), 7.7-7.75 (m, 2H), 7.84 (t, 1H), 8.1 (d, 1H), 10.5 (s, 1H); Mass Spectrum: M+H+292 and 294. |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| With triethylamine; In dichloromethane; water; | The N-(5-benzamido-2-chlorophenyl)-4-methoxy-2-nitrobenzamide used as a starting material was prepared as follows: Benzoyl chloride (5.2 ml) was added to a stirred mixture of 2,4-diaminochlorobenzene (6.42 g), triethylamine (12.5 ml) and methylene chloride (100 ml) which had been cooled to 0° C. The mixture was allowed to warm to ambient temperature and was stirred for 16 hours. The mixture was evaporated and the residue was triturated under a saturated aqueous sodium bicarbonate solution. The resultant solid was isolated, washed in turn with water and isohexane and dried under vacuum at 55° C. There was thus obtained N-(3-amino-4-chlorophenyl)benzamide as a solid (10.38 g); NMR Spectrum: (DMSOd6) 5.32 (s, 2H), 6.9 (m, 1H), 7.1 (d, 1H), 7.37 (d, 1H), 7.52 (m, 3H), 7.9 (d, 2H), 10.05 (s, 1H). |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| With triethylamine; In dichloromethane; water; | The N-(3-amino-4-chlorophenyl)benzamide used as a starting material was prepared as follows: Benzoyl chloride (5.2 ml) was added to a stirred mixture of 2,4-diaminochlorobenzene (6.42 g), triethylamine (12.5 ml) and methylene chloride (100 ml) which had been cooled to 0° C. The mixture was allowed to warm to ambient temperature and was stirred for 16 hours. The mixture was evaporated and the residue was triturated under a saturated aqueous sodium bicarbonate solution. The resultant solid was isolated, washed in turn with water and isohexane and dried under vacuum at 55° C. to yield the required compound (10.38 g); NMR 5.32 (s, 2H), 6.9 (m, 1H), 7.1 (d, 1H), 7.37 (d, 1H), 7.52 (m, 3H), 7.9 (d, 2H), 10.05 (s, 1H). |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| With triethylamine; In dichloromethane; | The N-(3-amino-4-chlorophenyl)-3-dimethylaminobenzamide used as a starting material was prepared as follows: A solution of 3-dimethylaminobenzoyl chloride hydrochloride (20 g) in methylene chloride (100 ml) was added dropwise over 2 hours to a solution of 4-chloro-3-aminoaniline (14.25 g) and triethylamine (38 ml) in methylene chloride (500 ml), and the reaction stirred at ambient temperature for 18 hours. The precipitated solid was collected to give the title compound (29.7 g, quantitative): NMR: 2.94 (s, 3H), 5.28 (s, 2H), 6.88 (m, 2H), 7.09 (d, 1H), 7.16 (m, 2H), 7.28 (dd, 1H), 7.33 (m, 1H), 9.91 (s, 1H); m/s 290, 292. |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| 72% | In n-heptane; water; ethyl acetate; toluene; | EXAMPLE 1 A room temperature solution of 1-chloro-2,4-diaminobenzene (1.43 g, 10 mmol), 1-hexadecanesulfonyl chloride (3.24 g, 10 mmol), imidazole (0.68 g, 10 mmol) and toluene (20 mL) was stirred for 1 hr. Isopropyl alcohol (20 mL) was added and the reaction mixture was heated at 60° C. for 2 hours. Analysis by thin layer chromatography (tlc) (40percent ethyl acetate and 60percent heptane) indicated incomplete acylation and additional 1-hexadecanesulfonyl chloride (0.7 g, 2 mmol) was added and the reaction mixture was stirred at 60°-62° C. for an additional hour, at which time the reaction appeared to be complete by tlc analysis. The reaction solution was treated with hot water (50 mL) and the layers separated. The toluene layer was further washed twice with 50 mL of hot water. Heptane (20 mL) was added to the recovered toluene layer to precipitate the product, N-(3-amino-4-chlorophenyl)-1-hexadecanesulfonamide, which was isolated by cooling the mixture to about 0° C. and collecting the product by vacuum filtration. After being washed with cold heptane, the product was dried (yield=3.12 g, 72percent of the theoretical yield) and it melted at 103°-104° C. No impurities were observed by H1 NMR analysis. |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| 98% | With N-ethyl-N,N-diisopropylamine; HATU; In N,N-dimethyl-formamide; at 25℃; for 10h; | Method 63; N-(3 - Amino-4-clilorophenyl)-3 -( 1 -cyano- 1 -methylethvDbenzamide; To a stirring solution of 3-(l-cyano-l-methylethyl)-benzoic acid (Method 73; 268 mg,1.41 mmol) in DMF (10 ml) were added 4-chlorobenzene-l,3-diamine (Method 61; 337 mg, 2.36 mmol), HATU (808 mg, 2.13 mmol) and DIEA (0.74 ml, 4.25 mmol) and the reaction mixture was stirred for 10 hours at 25 °C. The reaction mixture was partitioned between EtOAc and H2O. The organics were washed with H2O, brine and dried (MgSO4 (s)). The EPO <DP n="72"/>solvent was removed under reduced pressure affording 330 mg (98percent) of the title compound; m/z 314. |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| 95% | With 5%-palladium/activated carbon; hydrazine hydrate; In methanol; at 20℃; for 0.0833333h; | General procedure: To a mixture ofhalogenated nitroarene (1 mmol), Pd/C(5percent), and MeOH (5mL) was added NH2NH2·H2O (10 mmol), and the resultingsolution was heated at 80 °C reflux condition for 5 min. Thenthe mixture was filtered and concentrated in vacuo. Thecrude material was purified by flash column |
| 41% | With iron; ammonium chloride; In water; at 78℃; for 1h; | Method 61; 4-Chlorobenzene- 1 ,3-diamine; To a solution of ammonium chloride (1.57g, 29mmol) in H2O (10ml) were added 2- chloro-5-nitroaniline (1.Og, 5.8mmol) and iron powder (1.62 g, 29 mmol). The solution was stirred at 78 °C for one hour then filtered at 50 °C. The filtrate was collected and the solvent was removed under reduced pressure. The residue was taken up in DCM and filtered. The filtrate was collected and the solvent was removed under reduced pressure affording 337 mg of the title compound (41 percent); m/z 143. |
| 98%Chromat. | With hydrazine hydrate; In ethanol; at 80℃; for 1h;Inert atmosphere; | General procedure: Hydrazine hydrate was chosen as the hydrogen donor for the low emission of pollutants. In a typical procedure, hydrazine hydrate (4 equiv) was added into the reactor which containing fresh prepared catalyst as described above. Then the reactor was put into a preheated oil bath with a stirring speed of 500 rpm, and the substrate (1 mmol)dissolved in 1 mL ethanol was added drop-wisely under argon. The reactions were monitored by TLC. After the reaction, the reaction mixture was vacuum filtered through a pad of silica on a glass-fritted funnel and an additional 15 mL of ethyl acetate (5 mL portions) was used to rinse the product from the silica, the filtrate was concentrated in vacuum and analyzed by GC. Products were purified by column chromatography and identified by 1H NMR and 13C NMR. |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| With bromine; acetic acid; In methanol; | EXAMPLE 4 To a solution of 14.2 g of 4(5)-chloro-o-phenylene diamine and 38 g of ammonium rhodanide in 120 ml of methyl alcohol 5 ml of acetic acid are added, whereafter a solution of 7.2 ml of bromine in 20 ml of methyl alcohol is added at a temperature of 15°-17° C. in 30-60 minutes. The mixture is diluted with water and after adjusting the pH value to 8, 1,2-diamino-4-chloro-5-thiocyanatobenzene is obtained, melting at 108°-110° C. |
| Yield | Reaction Conditions | Operation in experiment |
|---|---|---|
| 31% | With 1H-imidazole; In dichloromethane; at 20℃; for 1h; | A mixture of4-chloro-1 ,3-phenylenediamine (0.500 g, 3.51 mmol), isopropylsulfonyl chloride (0.39 mL, 3.49 mmol), imidazole (0.240 g, 3.53 mmol) and dichloromethane (2 mL) was stirred 1 h at room temperature, then the mixture chromatographed(silica gel, ethyl acetate/hexane) to give the title compound (0.269 g, 31percent) as an oil. 1 H NMR (400 MHz, CHLOROFORM-d) delta ppm 1.38 (d, J=6.82 Hz, 6 H) 3.34 (septet, J=6.82 Hz1 1 H) 4.22 (s, 2 H) 6.54 (dd, J=8.59, 2.53 Hz, 1 H) 6.78 (d, J=2.53 Hz, 1 H) 7.14 (d, J=8.59 Hz, 1 H) 7.34 (s, 1 H) |